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Integrative analysis of the transcriptome and metabolome reveals the mechanism of saline-alkali stress tolerance in Astragalus membranaceus(Fisch)Bge.var.mongholicus(Bge.)Hsiao

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Food Quality and Safety 2022年第2期6卷 201-213页

作者：刘杰张雄杰盛晋华 Agricultural College Inner Mongolia Agricultural UniversityHohhotChina Inner Mongolia Horizon Oasis Research Center of Characteristic Biologic Resource HohhotChina

saline-alkali stress is a major abiotic stress affecting the quality and yield of crops.Astragalus membranaceus(Fisch)Bge.***.mongholicus(Bge.)Hsiao(Astragalus mongholicus(A.mongholicus))is a well-known medicine food homology species with various pharmacological effects and health benefits that can grow well in saline-alkali soil.However,the molecular mechanisms underlying the adaptation of A.mongholicus plants to saline-alkali stress have not yet been clarified.Here,A.mongholicus plants were exposed to long-term saline-alkali stress(200 mmol·L^(-1) mixed saline-alkali solution),which limited the growth of A.mongholicus.The roots of A.mongholicus could resist long-term saline-alkali stress by increasing the activity of antioxidant enzymes and the content of osmolytes.Transcriptome analysis(via the llumina platform)and metabolome analysis(via the Nexera UPLC Series QE Liquid Mass Coupling System)revealed that saline-alkali stress altered the activity of various metabolic pathways(e.***.amino acid metabolism,carbohydrate metabolism,lipid metabolism,and biosynthesis of other secondary metabolites).A total of 3690 differentially expressed genes(DEGs)and 997 differentially accumulated metabolites(DAMs)were identified in A..mongholicus roots under saline-alkali stress,and flavonoid-related DEGs and DAMs were significantly upregulated.Pearson correlation analysis revealed significant correlations between DEGs and DAMs related to flavonoid metabolism.MYB transcription factors might also contribute to the regulation of flavonoid biosynthesis.Overall,the results indicate that A.mongholicus plants adapt to saline-alkali stress by upregulating the biosynthesis of flavonoids,which enhances the medicinal value of A.mongholicus.

关键词： Astragalus mongholicus,transcriptome metabolome saline-alkali stress flavonoids

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Synergistic effect of Si and K in improving the growth,ion distribution and partitioning of Lolium perenne L.under saline-alkali stress

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Journal of Integrative Agriculture 2021年第6期20卷 1660-1673页

作者： FAN Yuan SHEN Wu-yan Pino VANESSA CHENG Fang-qin Institute of Resources and Environmental Engineering Shanxi University/State Environmental Protection Key Laboratory of Efficient Utilization Technology of Coal Waste ResourcesTaiyuan 030006P.R.China Sydney Institute of Agriculture School of Life and Environmental SciencesThe University of SydneyNSW 2006Australia

The application of Si or K has proven to be beneficial for the growth of plants under saline-alkali stress. However, the synergistic effect of Si and K in improving the growth, ion distribution, and partitioning in Lolium perenne L. under saline-alkali stress remains unclear. In this study, the growth characteristics and ion-selective absorption of ryegrass(Lolium perenne L.) exposed to different levels of saline-alkali stress were evaluated. The growth parameters of ryegrass were significantly improved when Si was applied by itself or coupled with K under low saline-alkali stress. Under a high saline-alkali level, only simultaneous application of Si and K could significantly improve the growth of ryegrass. When Si and K were applied together, the K^(+)/Na^(+) and Ca^(2+)/Na^(+) ratios in root, stem, and leaf of ryegrass were maximally improved as compared to the individual treatments and control. The K^(+) and Ca^(2+) concentrations in the vacuole, cell wall, and organelle of leaf were increased dramatically. This improvement was due to the ability of applied ions to compete with Na^(+), allowing the plant to maintain osmotic potential and leaf water content. The concentration of Na^(+) was significantly reduced when Si and K were applied and mainly concentrated in the soluble fraction and cell wall. The Si concentration in ryegrass increased markedly by the combined application of Si and K, and most of it was accumulated in the cell wall and soluble fraction, which could help in chlorophyll synthesis, reduce membrane injury, and increase water absorption under saline-alkali stress. This study emphasized the advantage of Si and/or K on the growth of plants under different saline-alkaline levels and provided a guide for the production of Si-K fertilizer and its application in saline-alkali soil.

关键词： saline-alkali stress Si K ryegrass plants K+/Na+ compartmentation

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Osmotic Regulation of Betaine Content in Leymus chinensis Under saline-alkali stress and Cloning and Expression of Betaine Aldehyde Dehydrogenase(BADH) Gene

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Chemical Research in Chinese Universities 2008年第2期24卷 204-209页

作者： CUI Xi-yan WANG Yong GUO Ji-xun Key Laboratory for Vegetation Ecology of Ministry of Education Institute of Grassland Science Northeast Normal University Changchun 130024 P. R. China School of Life Sciences Jilin Agricultural University Changchun 130118 P. R. China

The potted Leymus chinensis seedlings were treated with saline-alkali solution of six different（from Ⅰ to Ⅵ） concentrations. The results demonstrate that the betaine content and Betaine-aldehyde dehydrogenase（BADH： EC 1.***.1.8） activities have a direct relation with increased stressing time in the same treatment; both exhibit a single peak with increasing the concentration of saline-alkali solution, and number V shows the highest value. The BADH gene of Leyrnus chinensis was cloned by RT-PCR and RACE technology and was designated as LcBADH. The cDNA sequence of LcBADH was 1774bp including the open reading frame（ORF） of 1521bp（coding 506 amino acids）. The vector of prokaryotic expression was constructed by inserting the LcBADH gene fragmcnt into pET30a（＋） and transformed into E. coli BL21（DE3）. The result of SDS-PAGE shows that the idio-protein with a molecular mass of 56.78 kDa was effectively expressed in the recombinant bacteria induced by isopropyl fl-D-thiogalactoside（IPTG）.

关键词： Leymus chinensis saline-alkali stress BADH RACE Cloning and expression

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Effect of saline-alkali stress on Seed Germination and Seedling Growth of Oat

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Plant Diseases and Pests 2015年第2期6卷 26-29,41页

作者： Wang Guijun Wang Lei Meng Xuan Xu Zhenwen College of Urban and Environmental Sciences Changchun Normal University College of Life Sciences Northeast Normal University

Neutral black soil was mixed with strong saline-alkali soil in different weight ratios, then physiological indexes during seed germination and seedling growth in soil of different ratios and the corresponding leaching liquor treatments were observed to explore effect of saline-alkali stress on oat seed germination and seedling growth, and analyze tolerance of oat to saline-alkali soil. The results showed that germination ability of oat seeds reduced with the increasing saline-alkali stress, salt injury index increased and seedling growth was inhibited. Effect of different saline-alkali stresses on germination ability of oat seeds showed difference, and effect of leaching liquor treatment on oat seed germination was stronger than that of soil treatment.

关键词： saline-alkali stress Oat Seed germination Growth index Inhibition

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Cloning and Expression Analysis of THWRKY12 Gene from Tamarix hispida

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Agricultural Biotechnology 2013年第5期2卷 55-57页

作者： Jingxue WANG Yuekun TANG Qi LIU Chunqiang LIANG Yucheng WANG State Key Laboratory of Forest Genetics and Tree Breeding Northeast Forestry University

[ Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaC1 solutlon, 20% PEG and 100 μmol/L ABA, respectively. The expression of THIVRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaC1 and PEG, the expression of THWRKY12 gene in different tissues of T. h/sp/da seedlings showed an overall upregulated trend, suggesting that THWRKYI2 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-al- kali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [ Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida.

关键词： Tamarix hispida WRKY transcription factor RT-PCR saline-alkali stress Drought stress

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Research Progress on the Mechanism of Crop saline-alkali Tolerance and Mitigation Measures

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Agricultural Biotechnology 2021年第5期10卷 74-78页

作者： Ting XU Yantao LIU Agricultural College of Shihezi University Shihezi 832000China

The wide distribution of saline-alkali land in China is a restrictive factor for the sustainable development of agriculture.saline-alkaline soil inhibits the growth and development of crops,reducing its yield and quality.In this article,we summarized the germination status,physiological characteristics,response mechanisms and mitigation measures of different crops under saline-alkali stress in recent years,aiming to provide important reference for the study of saline-alkali tolerance mechanism in crops,cultivation of crop varieties tolerant to salts and alkalis and improvement of the utilization rate of saline-alkali land,and put forward suggestions for future development trend of saline-alkali land crops and mitigation measures.

关键词： saline-alkali stress Crop Response mechanism Mitigation measures Research progress

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