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Genetic Variation in Picea mariana ×P. rubens Hybrid Populations Assessed with ISSR and RAPD Markers

American Journal of Plant Sciences 2012年第6期3卷 731-737页

作者： Ramya Narendrula Kabwe Nkongolo Department of Biology Laurentian University Sudbury Canada

Interspecific hybridization can result in significant shifts in allele frequencies. The objective of the present study was to assess the level of genetic variation in populations of P. mariana × P. rubens hybrids derived from artificial crosses. Progenies from backcross populations created through a series of controlled pollinations among P. mariana and P. rubens trees across the hybridization index were analyzed. Several Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) primers were used to amplify genomic DNA samples from each population. ISSR primers produced from 30% to 52% polymorphic loci. The level of polymorphism was higher with RAPD markers, ranging from 57% to 76%. Overall, the two marker systems generated similar levels of polymorphic loci for P. mariana and P. rubens populations. No significant differences were found among the P. mariana × P. rubens populations analyzed and between the hybrids and the parental populations regardless of the molecular marker used. This confirms the genetic closeness of P. mariana and P. rubens species.

关键词： Picea mariana Picea rubens Interspecific Hybrids Inter Simple Sequence Repeat Random Amplified Polymorphic DNA

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Chemical Constituents of the Root Essential Oils of <i>Zingiber rubens</i>Roxb., and <i>Zingiber zerumbet</i>(L.) Smith

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American Journal of Plant Sciences 2013年第1期4卷 7-10页

作者： Do N. Dai Tran D. Thang Le T. M. Chau Isiaka A. Ogunwande Faculty of Biology Vinh University Vinh City Vietnam Faculty of Chemistry Vinh University Vinh City Vietnam Natural Products Research Unit Department of Chemistry Faculty of Science Lagos State University Lagos Nigeria

Zingiber rubens Roxb., and Zingiber zerumbet (L.) Smith were collected respectively from Nghe An, Province and Ha Tinh, Province,Vietnam. The root essential oils were obtained by water distillation and analyzed by Gas chromatography (GC) and Gas chromatography coupled with Mass spectrometry (GC-MS). The most abundant components of Z. rubens were (Z)-citral (30.1%), camphene (9.7%), β-phellandrene (7.5%) and 1,8-cineole (7.0%) and zingiberene (5.3%). The main oil constituents of Z. zerumbet were (Z)-citral (26.1%), camphene (16.3%), sabinene (14.6%), zingiberene (7.2%) and lavandulyl acetate (6.7%). This species has low zerumbone (1.2%) content.

关键词： Zingiber rubens Zingiber zerumbet Root Essential Oil (Z)-Citral Camphene Sabinene Zerumbone

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The Recent Letter from Professor rubens Belfort Jr. President, the World Ophthalmology Congress （WOC） 2006

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国际眼科杂志 2005年第3期5卷 427-427页

作者： rubensBelfort

Dear Prof. Xiu-Wen Hu,We are very pleased for your participation in the World Ophthalmology Congress. We already look for the great one China will organize for 2006! We are pleased to inform you that Prof. Harley Bicas, coordinator of the symposium and probably President of the Brazilian Council of Ophthalmology for next year has included your presentation “Development of Ophthalmological Academic Periodicals in China” at the World Forum of Editors in Ophthalmology.

关键词： rubens Belfort Jr 行长眼肌麻痹 WOC 2006年杂志

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