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Cystathionine-β-synthase gene transfer into rostral ventrolateral medulla exacerbates hypertension via nitric oxide in spontaneously hypertensive rats

Cystathionine-β-synthase gene transfer into rostral ventrol...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：武宇明段肖翠刘尚雨郭蓉肖琳薛红梅郭琪河北医科大学生理研究室河北医科大学附属第三医院教务处

Hydrogen sulfide（H2S）is known as a novel signaling molecule endogenously produced to influence cardiovascular regulation in the central nervous ***-β-synthase（CBS）is a major H 2S-generating enzyme that has been identified in the *** ventrolateral medulla（RVLM）plays crucial roles in central cardiovascular *** expression is decreased in the RVLM inspontaneously hypertensive rat（SHR）compared with Wistar-Kyoto rat（WKY）.However,the chronic role of H2S on cardiovascular regulation in the RVLM in SHR remains *** evidences suggest that H2S interacts with nitric oxide（NO）to regulate cardiovascular ***,we hypothesize that decreased CBS in the RVLM may be involved in the disorder L-arginine/NO pathway and subsequently affects blood pressure in *** of CBS in the RVLM by recombinantadenovirus vectors encoding CBS（Ad CBS）caused significant increases in systolic blood pressure and heart rate in SHR but not in WKY *** experiments were carried out at 7 day after gene *** the RVLM,NOx level was decreased whereas urinary norepinephrine level was increased in SHR,which were aggravated after CBS gene ***,pressor responses to microinjection of N G-monomethyl-L-arginine into RVLM was blunt in SHR by CBS overexpression,but not in WKY *** together,these findings suggest that pressor responses to overexpression CBS were mediated by the *** CBS in the RVLM of SHR may be an important compensatory mechanism in hypertension which contributes to inhibition of further blood pressure *** findings provide new perspectives on the mechanisms of blood pressure regulation in hypertension.

关键词： Hypertension Cystathionine β-synthase rostral ventrolateral medulla Nitric oxide

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Exercise training normalizes the imbalance between AngⅡand Ang 1-7 in the rostral ventrolateral medulla of hypertensive rats

Exercise training normalizes the imbalance between AngⅡand ...

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2012国际生理学学术大会

作者： Yang-kai Wang Wen-jun Yuan Wei-zhong Wang Department of physiology Second militarymedical university

＜正＞Imbalance between angiotensinⅡ（AngⅡ） and Ang 1-7 in the brain has been reported to be associated with *** training（ExT） is capable of reducing blood pressure in *** study is aimed to determine if exercise training（ExT） improves hypertension via adjusting central rennin angiotensin *** hypertensive rats（SHRs） were submitted to training or kept sedentary for 3 *** pressure elevation coupled with increase in ages was significantly decreased in SHR received *** results from in vivo study showed that ExT significantly reduced or increased the cardiovascular response to central application of AngⅡor Ang 1-7,*** data from western blot suggested that protein expression of the AngⅡacting receptor AT1 and the Angl-7 acting receptor Mas in the RVLM,a key region for control of sympathetic outflow,was significantly reduced and elevated in SHR following ExT,*** also found that ExT decreased and increased the concentration of AngⅡand Ang 1-7 in the RVLM of *** work has confirmed that ExT improves hypertension via resetting the balance ofAngⅡand Ang 1-7 in the RVLM.

关键词： AngⅡ/Ang 1-7 training hypertension rostral ventrolateral medulla

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Effects of electroacupuncture on supraoptic nucleus neurons and release of amino acids from rostral ventrolateral medulla in surgical trauma rats

Effects of electroacupuncture on supraoptic nucleus neurons ...

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中国神经科学学会第十届全国学术会议

作者： Huan-Huan ZHANG Mei-Yan JIANG Jin WANG Fen XIAO Jun CHEN Chun-Mei XIA Meng-Ya WANG Da-Nian ZHU Department of Physiology and Pathophysiology Shanghai Medical College of Fudan University Cell Electrophysiology Laboratory Wannan Medical College

Objective To investigate the effect of electroacupuncture（EA） on supraoptic nucleus（SON） and the release of amino acids from the rostral ventrolateral medulla（RVLM） in surgical trauma stressed rats,and explore the central mechanisms underlying electroacupuncture *** Animals were randomly divided into non-stressed group（Control）,surgical trauma stressed group（Trauma）,Neiguan EA applied on surgical trauma stressed group（Trauma+EA PC 6）.The immunohistochemistry for c-Fos expression was made in SON neurons,and microdialysis combining HPLC-fluorescence detection（HPLC-FD） was used to test the amino acids in *** At 60 min after surgical trauma,the expression of c-fos increased significantly in SON of trauma rats as compared with Control（P＜ 0.05,n = 4）,which was dramatically prevented when EA was applied on trauma rats（P ＜ 0.05,n = 4）.In Trauma group,glutamate（Glu） and taurine（Tau） in the dialysate from the RVLM simultaneously increased in comparison with *** decreased Glu and Tau in Trauma+EA PC 6 *** These results indicate that the SON neurons and the amino acid neurotransmitters in RVLM may be involved in regulation of cardiac function when Neiguan EA was applied on surgical trauma rats.

关键词： electroacupuncture surgical trauma supraoptic nucleus rostral ventrolateral medulla

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Possible functional connection between supraoptic nucleus and cardiovascular-related neurons in rostral ventrolateral medulla of rats

Possible functional connection between supraoptic nucleus an...

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中国神经科学学会第十二届全国学术会议

作者： Yi-Nan TAO Chao ZHENG Wen QING Huan-Huan ZHANG Meng-Ya WANG Cell Electrophysiology Laboratory Wannan Medical College

Objective To explore the possible functional connection between supraoptic nucleus（SON） and cardiovascular-related neurons in rostral ventrolateral medulla（RVLM） of rats. Methods By using the technology of extracellular recording in RVLM and simultaneous recording of physiological functions, the effects of SON stimulation were observed on discharges of cardiovascular related neurons in the RVLM and physiological functions of anesthetized adult male Sprague-Dawley rats. The VP receptor（V1 a） blocker SR49059 was microinjected into RVLM nucleus to test the antagonist action. Results Nineteen spontaneous unit discharges were recorded from 18 rats. After electrical stimulation（200μA, 0.2 ms, 100 Hz, 0.2 s） of SON, the discharge frequency of 10 RVLM neuron units decreased significantly（P＜0.05）, and corresponding mean arterial pressure increased（P＜0.05）, but heart rate and respiratory rate only had a decreasing trend. However, discharge frequency in 9 RVLM neuron units increased（P＜0.05）. In 9 RVLM neuron units with the decreases in the discharge frequency（P＜0.05） induced by electrical stimulation of SON, the SON stimulation effect was not significant after microinjection of SR49059 into RVLM for 10 min. Conclusions These preliminary results indicate that direct or indirect functional connection may exist between SON and RVLM, and VP may be involved in the functional connection.

关键词： supraoptic nucleus rostral ventrolateral medulla extracellular recording technique polygraph cardiovascular functions vasopressin

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Superoxide Depression in the rostral ventrolateral medulla Contributes to Centrally Antihyper-tensive Action of Moxonidine

Superoxide Depression in the Rostral Ventrolateral Medulla C...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者： Yangkai Wang Weizhong Wang Department of Physiology Second Military Medical University

BACKGROUND AND PURPOSE:It has been demonstrated that centrally antihypertensive action of moxonidine is resulted from activation of Imidazoline-1 receptor（I1R）in the rostral ventrolateral medulla（RVLM）.Increased superoxide in the RVLM contributes to high level of blood pressure and sympathetic outflow in *** objective of the present study was to determine the effect of moxonidine onproduction of superoxide in the RVLM of spontaneously hypertensive rat（SHR）.EXPERIMENTAL APPROACH Sixteen-week old SHR that received intracerebroventricular（icv,2 weeks）infusion of the tested agents was subjected to subsequent *** cardiovascular parameters including blood pressure,heart rate,and renal sympathetic nerve activity were monitored by Powerlab system（ADInstrument,Inc）.RVLM tissue was collected for assessment of protein and m RNA expression by western blotting and *** production of superoxide in the RVLM was measured using dihydroethidium fluorescence *** for moxonidine’s effect on superoxide was investigated by lentivirus infection in the *** RESULTS ICV infusion of moxonidine（20 nmol/day,2 weeks）produced a significant decrease in BP（143±3 vs 186±5 mm Hg）in SHR,which can be prevented by the I1R antagonist efaroxan but not the alpha 2 receptor antagonist *** production of ROS in the RVLM was significantly reduced by an average of 37%in the SHR with moxonidine infusion compared with vehicle *** significantly blunted the decrease and increase in BP,HR,and RSNA evoked by injection of the SOD mimic Tempol（5 nmol）and Angiotensin Ⅱ（20 pmol）into the RVLM,*** expression of NADPHase（NOX4）and angiotensin type I receptor（AT1R）in the RVLM was significantly decreased in SHR with infusion of moxonidine.B ***?Moxonidine also significantly inactivated the PI3K-Akt-NF of lentivirus containing PI3K sh RNA in the RVLM effectively prevented effects of *** AND?moxonidine on BP

关键词： Centrally antihypertensive mechanism superoxide PI3K rostral ventrolateral medulla

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延髓头端腹外侧区在电针预处理减轻心肌缺血再灌注损伤中的作用

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World Journal of Acupuncture-Moxibustion 2023年第2期33卷 142-149页

作者：余情吴立斌张帆魏小桐乙春梅蔡荣林胡玲安徽中医药大学针灸推拿学院安徽合肥230012 安徽省中医药科学院针灸经络研究所安徽合肥230038 新安医学教育部重点实验室安徽合肥230038

ObjectiveTo observe the effects of pretreatment with electroacupuncture (EA) on neuron activity in the rostral ventrolateral medulla (RVLM) of rats with myocardial ischemia-reperfusion injury (MIRI) and explore the central regulatory mechanism of EA in attenuating *** 72 SD rats, 12 were randomly allocated into the group of EA pretreatment + RVLM nucleus damage (EA + RVLM lesion group). The other 60 rats were randomized (20 rats each) into a sham-operation group, a model group, and an EA pretreatment group (EA group). Except for the rats in the sham-operation group, the models of MIRI were prepared by ligating the left anterior descending coronary artery in the model, EA, and EA + RVLM lesion groups. The rats of the EA group were intervened with EA at “Shénmén (神门HT7)” and “Tōnglĭ (通里HT5)”, 1 mA in current intensity and 2 Hz in frequency, for 20 min each time per day. Before modeling, the intervention was given for seven consecutive days. In the EA + RVLM lesion group, 3 weeks after microinjection with the neuronal apoptotic virus at bilateral RVLM, the same EA intervention as the EA group was provided. Afterward, the MIRI models were prepared. In the model group, no EA intervention was given. Using Powerlab electrophysiolograph, ST segment displacement value and arrhythmia score were recorded and analyzed before modeling, 30 min after ligation, and 120 min after reperfusion in each group. The concentration of cardiac troponin (cTnl) was detected with an ELISA assay kit. Using immunofluorescence staining, the expression level of c-fos protein of RVLM was detected in the sham-operation, model, and EA groups separately. Plexon multichannel acquisition processor was adopted to record the neuronal firing and field potential of RVLM in the sham-operation, model, and EA *** segment displacement value, arrhythmia score, and cTnl concentration 30 min after ligation and 120 min after reperfusion were all elevated in the model group compared to the sha

关键词： Electroacupuncture Myocardial ischemia-reperfusion injury rostral ventrolateral medulla Neural mechanism

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维普期刊数据库

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Circuit-Specific Control of Blood Pressure by PNMT‑Expressing Nucleus Tractus Solitarii Neurons

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神经科学通报:英文版 2023年第8期39卷 1193-1209页

作者： Shirui Jun Xianhong Ou Luo Shi Hongxiao Yu Tianjiao Deng Jinting Chen Xiaojun Nie Yinchao Hao Yishuo Shi Wei Liu Yanming Tian Sheng Wang Fang Yuan Department of Neurobiology Hebei Medical UniversityShijiazhuang 050017China Institute of Cardiovascular Research Southwest Medical UniversityLuzhou 646000China Core Facilities and Centers Institute of Medicine and HealthHebei Medical UniversityShijiazhuang 050017China Hebei Key Laboratory of Neurophysiology Shijiazhuang 050017China

The nucleus tractus solitarii(NTS)is one of the morphologically and functionally defined centers that engage in the autonomic regulation of cardiovascular ***-characterized NTS neurons have been implicated in the differential regulation of blood pressure(BP).Here,we investigated whether phenylethanolamine N-methyltransferase(PNMT)-expressing NTS(NTS^(PNMT))neurons contribute to the control of *** demonstrate that photostimulation of NTS^(PNMT)neurons has variable effects on BP.A depressor response was produced during optogenetic stimulation of NTS^(PNMT)neurons projecting to the paraventricular nucleus of the hypothalamus,lateral parabrachial nucleus,and caudal ventrolateral ***,photostimulation of NTS^(PNMT)neurons projecting to the rostral ventrolateral medulla produced a robust pressor response and *** addition,genetic ablation of both NTS^(PNMT)neurons and those projecting to the rostral ventrolateral medulla impaired the arterial ***,we revealed the neuronal phenotype-and circuit-specific mechanisms underlying the contribution of NTS^(PNMT)neurons to the regulation of BP.

关键词： Nucleus tractus solitarii Blood pressure rostral ventrolateral medulla Optogenetics Neural circuit

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Effects of electroacupuncture on cardiac function and c-fos expression in the central nervous system in surgical trauma rats

Effects of electroacupuncture on cardiac function and c-fos ...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Huan-Huan ZHANG~(1,2),Jun CHEN~1,Chun-Mei XIA~1,Jin WANG~1,Yin-Xiang Cao~1,Li LI~1,Lin-Lin SHEN~1,Da-Nian ZHU~(1,*) 1 Department of Physiology and Pathophysiology,Shanghai Medical College of Fudan University,Shanghai 200032, China 2 Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,China

＜正＞Objective The present study was carried out to investigate the effect of electroacupuncture（EA） on cardiac function in surgical trauma stressed rats,and further study the mechanisms underlying the *** Animals were randomly divided into non-stressed group（Control）,surgical trauma stressed group（Trauma）,EA applied on surgical trauma stressed group（Trauma+EA）.The effect of EA on cardiac function of thoracotomy trauma rats was investigated by detecting blood pressure and intraventricular pressure,as well as immunohistochemistry for c-fos protein expression in *** It was shown that surgical trauma stress dramatically attenuated cardiac *** 60 min after surgical trauma,as compared with those before thoracotomy trauma,almost all parameters of the cardiac function decreased significantly,including heart rate（HR）（P＜0.01,n=7）,mean arterial pressure（MAP）（P＜0.05,n=7）,maximal rate of intraventricular pressure rising（+dP/dtmax）（P＜0.05,n=7） and declining（-dP/dtmax）（P＜0.05,n=7）,and the total area of cardiac loop（L0）（P＜0.05,n=7）.Significant decreases in the HR（P＜0.01,n=1） and -dP/dtmax（P＜0.05,n=7） at least remained to 120 min after surgical ***,EA（with frequency of 5 Hz,duration of 0.5 ms and the intensity of 4 mA,just strong enough to elicit slight twitches of the hindlimb） at bilateral Neiguan acupoints（Pe 6） suppressed the decreases in HR,MAP and other parameters of cardiac *** the other hand,surgical trauma has a significant effect on neuronal responses in the paraventricular nucleus（PVN） and the rostral ventrolateral medulla（RVLM）,which is accompanied by those cardiac responses,*** 60 min after surgical trauma,it was found a significant increase in the expression of c-fos in the PVN and the RVLM,respectively,in the trauma rats as compared with that in the control animals（P＜0.05,n=6）,however,the number of Fos- immunoreactive neurons of the PVN and the RVLM dramatically decreased when EA was applied on tra

关键词： electroacupuncture surgical trauma cardiac function c-fos paraventricular nucleus rostral ventrolateral medulla

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Gene transfer of cystathionine β-synthase into RVLM increases hydrogen sulfide-mediatedsuppression of sympathetic outflow via KATP channel in normotensive rats

Gene transfer of cystathionine β-synthase into RVLM increas...

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中国生理学会张锡钧基金会第十三届全国青年优秀生理学学术论文交流及评奖会议、中国生理学会第十一届全国青年生理学工作者学术会议

作者： Xiao-cui Duan Rong Guo Shang-yu Liu Lin Xiao Hong-mei Xue Qi Guo Sheng Jin Yu-ming Wu Department of Physiology Institute of Basic MedicineHebei Medical University Hebei Key Lab of Laboratory Animal Science Department of Laboratory Animal ScienceHebei Medical University Dean’s Office of the Third Hospital of Hebei Medical University

Hydrogen sulfide（H S） has been shown to have a sympathoinhibitory effect in the rostral ventrolateral medulla（RVLM）.The present study examined the function of cystathionine-P-synthase（CBS）/H S system in the RVLM which plays a crucial role in the control of blood pressure and sympathetic nerve *** vectors encoding CBS（AdCBS） or enhanced green fluorescent protein（AdEGFP） were transfected into the RVLM in normotensive *** microinjection of AdCBS into the RVLM had no effect on systolic blood pressure and heart rate（HR） in conscious *** experiments were performed at day 7 after gene transfer in anesthetized *** of the CBS inhibitors,hydroxylamine（HA） or amino-oxyacetate,into the RVLM produced an increase in the renal sympathetic nerve activity（RSNA）,mean arterial pressure（MAP） and *** was a potentiation of the increases in RSNA,MAP,and HR due to the CBS inhibitors in AdCBS injected rats compared to AdEGFP injected *** with pinacidil,a K channel activator,abolished the effects of HA in two *** of glibenclamide,a K channel blocker,produced increases in RSNA,MAP,and HR in AdCBS injected *** changes in behavior were observed in AdEGFP injected ***,Western blot analysis indicated an increase in the expression of SUR2 and Kir6.1 in AdCBS injected *** results suggest that the increase in K channels in the RVLM may be responsible for the greater sympathetic outflow and pressor effect of HA in AdCBS injected rats compared to AdEGFP injected rats.

关键词： cystathionine β-synthase rostral ventrolateral medulla renal sympathetic nerve activity gene transfer blood pressure

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