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A survey on Hibiscus rosa-sinensis,Alcea rosea L.and Malva neglecta Wallr as antibacterial agents

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Asian Pacific Journal of Tropical Medicine 2010年第5期3卷 351-355页

作者： Seyyed Mansour Seyyednejad Haniyeh Koochak Esmaeil Darabpour Hossein Motamedi Department of Biology Faculty of ScienceShahid Chamran University

Objective:To guide for selection of plants with antibacterial activity for further phytochemical works on the isolation and identification of the active compounds.Methods:Ethanolic extracts of 3 species from Malvaceae family were evaluated by agar disc diffusion method for antibacterial activity against some gram-positive and gram-negative bacteria(Pseudomonas aeruginosa,Listeria monocytogenes,Staphylococcus epidermidis.Staphylococcus aureus, Klebsiella pneumoniae.Salmonella typhi,Bacillus cereus.Bacillus anthracis,Escherichia coli and Streptococcus pyogenes).The extracts were obtained from aerial parts of Hibiscus rosa(H. rosa)-sinensis(leaf and flower),Alcea rosea(A.rosea) L.(leaf and flower) and Malva neglecta(M. neglecta) Wallr(flower).Results:These extracts had inhibitory effects at different concentrations (0.05,0.10,0.20 and 0.40 g/mL) against above mentioned bacteria.Escherichia coli was the most resistant strain.The highest inhibitory zone was showed by ethanolic extract of M.neglecta against Staphylococcus epidermidis(22 mm) and followed by ethanolic extract from flower of H.rosa against Staphylococcus epidermidis and Staphylococcus aureus(20 mm).The Minimum Inhibitory Concentration(MIC) and Minimum Bactericidal Concentration(MBC) values against Staphylococcus epidermidis were equal(M1C=MBC=5 mg/mL for M.neglecta extract and for H. rosa extract MIC=MBC=20 mg/mL).Conclusions:These findings suggest that these native plants have good antibacterial properties that can be used for infection control and treatment and could also be as new source for antibiotics discovery and infection treatment.

关键词： Alcea rosea Antibacterial activity Ethanolic extract Hibiscus rosa-sinensis Malva neglecta

Antileishmanial assessment of leaf extracts from Pluchea carolinensis,Pluchea odorata and Pluchea rosea

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Asian Pacific Journal of Tropical Medicine 2011年第10期4卷 836-840页

作者： Marley Garcia Wilmer H Perera Ramon Scull Lianet Monzote Parasitology Department Institute of Medicine Tropical "Pedro Kourl"Havana CityCuba Institute of Ecology and Systematic Havana CityCuba Institute of Pharmacy and Food Havana CityCuba Parasitology Department Institute of Medicine Tropical "Pedro Kouri"Havana CityCuba

Objective:To evaluate the antileishmanial activity of different extracts from three Cuban Pluchea species.Methods:In in vitro assays the IC50was calculated in the promastigotes and amastigotes forms as cytotoxicity in murine macrophages.In leishmaniasis cutanea experiment,mortality, weight loss,lesion size and burden parasite were measured.Results:Extracts evaluated showed inhibitive effect on growing of promastigote form;however,active extracts caused a high toxicity. Ethanol and n-hexane extracts demonstrated specific antileishmanial activity.Ethanol and n-hexane extracts from Pluchea carolinensis（P.carolinensis） caused similar inhibition against amastigote form.The intraperitoneal administration of the ethanol extract of P.carolinensis at 100 mg/kg prevented lesion development compared with control groups.Conclusions:The antileishmanial experiment suggests that ethanol extracts from P.carolinensis is the most promising.Further studies are still needed to evaluate the polential of this plant as a source of new antileishmanial agents.

关键词： Pluchea carolinensis Pluchea odorata Pluchea rosea Medicinal plants Leishmania amazonensis

In silico analysis and experimental validation of active compounds from Rhodiola rosea L. ameliorating pulmonary fibrosis

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中国药理学通报 2015年第B11期31卷 143-143页

作者： ZHANGKe HUANGJian LIGuo-yu WANGHang-yu WANGJin-hui SchoolofTraditionalChineseMateriaMedica ShenyangPharmaceuticalUniversitySheyangLiaoning110016 SchoolofPharmacy ShiheziUniversityShiheziXijiang832002

Aim Rhodiola rosea L. possesses a wide range of pharmacological properties including lung-protective, and it has been implemented in folk medicine for several 100 years. However, the accurate mechanisms of its lung- protective activity remain unclear. This study aimed at investigating the possible mechanisms of lung-protective activity of Rhodiola rosea L. in pulmonary fibrosis model. Methods Pathological observation, ROS detection and measure- ments of biochemical indexes on rat models proved lung-protective effect of Rhodiola rosea L. Identification of active compounds in Rhodiola rosea L. was executed through several methods including UPLC-TOF-MS. SEA docking, too- lecular modeling, molecular docking, and molecular dynamics （MD） simulation were applied in this study to explore possible mechanisms of the lung-protective potential of Rhodiola rosea L. Furthermore, in vitro cytological examina- tion and Western blot were used for validating the efficacy of the selected compounds. Results Experiments on rat models showed a potent lung-protective effect of Rhodiola rosea L. Then we analyzed the chemical composition of Rhodiola rosea L. and found out their key targets. Moreover, in silico analysis results testified good interaction be- tween selected compound 13 and key targets Akt-1/Caspase-1, and compound 10 also interacts well with Akt-1. Fur- ther Western blot analysis proved changed expression levels of those target proteins, indicating that selected small compounds indeed acted on those targets. Conclusion In silico analysis and experimental validation together demon- strated that selected compound 10 in Rhodiola rosea L. targeted Akt-1 in hepatocytes. Besides, compound 13 targeted both Caspase-1 and Akt-1. These small compounds may ameliorate pulmonary fibrosis by acting on their targets which are related to apoptosis or autophagy. The conclusions above may shed light on the complex molecular mechanisms of Rhodiola rosea L. acting on pneumonocyte and ameliorating pulmonary fibrosis.

关键词： Rhodiola rosea L. in silico analysis pulmonary fibrosis target Akt-1 Caspase-1.

Protective effect of thodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury

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Neural Regeneration Research 2020年第4期15卷 690-696页

作者： Xiao-Qin Ha Bo Yang Huai-Jing Hou Xiao-Ling Cai Wan-Yuan Xiong Xu-Pan Wei Lanzhou University Second Hospital LanzhouGansu ProvinceChina Department of Clinical Laboratory Lanzhou General Hospital of Lanzhou Military Area CommandLanzhouGansu ProvinceChina School of Clinical Medicine Gansu University of Traditional Chinese MedicineLanzhouGansu ProvinceChina School of Life Science and Engineering Lanzhou University of TechnologyLanzhouGansu ProvinceChina

Rhodioloside has been shown to protect cells from hypoxia injury,and bone marrow mesenchymal stem cells have a good effect on tissue repair.To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury,a rat model of spinal cord injury was established using the Infinite Horizons method.After establishing the model,the rats were randomly divided into five groups.Rats in the control group were intragastrically injected with phosphate buffered saline(PBS)(5μL).PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm.Rats in the rhodioloside group were intragastrically injected with rhodioloside(5 g/kg)and intramuscularly injected with PBS.Rats in the mesenchymal stem cell(MSC)group were intramuscularly injected with PBS and intramuscularly with MSCs(8×10^6/mL in a 50-μL cell suspension).Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs.Rats in the rhodioloside+Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside.One week after treatment,exercise recovery was evaluated with a modified combined behavioral score scale.Hematoxylin-eosin staining and Pischingert’s methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue.Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord.Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord.The results showed that:(1)compared with the other groups,the rhodioloside+Ad-HIF-MSC group exhibited the highest combined behavioral score(P<0.05),the most recovered tissue,and the greatest number of neurons,as indicated by Pischingert’s methylene blue staining.(2)Compared with the PBS group,HIF-1 protein ex

关键词： acute spinal cord injury adenovirus adenovirus gene IX bone marrow mesenchymal stem cells combined behavioral score scale HIF-1α nerve regeneration nerve repair Rhodiola rosea Sry

渤海红海葵的生物医学研究——Ⅲ、Sagartia rosea活性蛋白特性的初步分析

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中国海洋药物 1995年第4期14卷 10-14页

作者：陆章铿张亚雄范振环交通部石油部海洋水下工程科学研究院上海200231

据世界有毒海洋动物志记载,曾有2篇关于 Sagartia rosea(elegans)Gosse伤人的典型报道,分别发生在法兰西的冰岛至大西洋海岸,以及地中海与非州海岸(White(1934)与Southcott(1963))。

关键词：渤海红海葵 Sagartia rosea 蛋白质

Enhancement of defense responses by <i>Clonostachys rosea</i>against Botrytis <i>cinerea</i>in tomatoes

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Agricultural Sciences 2013年第12期4卷 709-714页

作者： Liana Dalcantara Ongouya Mouekouba Aijie Wang Zhenzhu Zhang Kehinde O. Erinle Xiuling Chen Aoxue Wang Colleges of Life Sciences Northeast Agricultural University Harbin China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin China Colleges of Life Sciences Northeast Agricultural University Harbin China

Clonostachys rosea (C. rosea) is a biocontrol agent that is used to combat and prevent phytopathogenic fungi attacks because of its ability to involve many factors and diverse modes of action. The reactions of C. rosea on the control of gray mold disease in tomato leaves were investigated in this study. To investigate the reactions of C. rosea in inducing resistance to tomato plants, three treatments, including Botrytis cinerea treatment (treatment B), C. rosea treatment (treatment C), C. rosea and B. cinerea treatment (treatment C + B) and water (control), to be applied on tomato leaves were set up. Disease severity was subsequently evaluated and compared with the control. The treatment of tomato leaves with C. rosea (15 μg/ml) significantly reduced the disease index after inoculation and severity of gray mold caused by Botrytis cinerea. The results indicated that the C. rosea treatment stimulated the activity of the defense related enzymes: Peroxidases (POX), lipoxygenases (LOX) and glutathione S-transferases (GST), and the treatment C + B reduced the incidence and severity of the gray mold. Furthermore, C. rosea treatment increased the activity of pathogenesis related proteins PR1. Therefore, our results suggest that C. rosea could enhance the resistance of tomato plants to gray mold through the activation of defense genes and via the enhancement of defense-related enzymatic activities.

关键词： Botrytis cinerea Clonostachys rosea Defense Enzymes Glutathione S-Transferases Li-poxygenases Peroxidases Pathogenesis Related

拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估(英文)

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南京林业大学学报（自然科学版） 2009年第1期33卷 1-10页

作者：李德伟 John C.Sutton 加拿大圭尔夫大学环境生物系

以仙客来叶圆片方法对灰霉菌Botrytis cinerea的生防效果进行了评估,结果发现Clonostachys rosea和Trichoderma koningii抑制B.cinerea的效果达94%～100%,其效果优于所测试的从仙客来上分离的真菌和细菌的多数菌株。用整株仙客来测试生... 详细信息

以仙客来叶圆片方法对灰霉菌Botrytis cinerea的生防效果进行了评估,结果发现Clonostachys rosea和Trichoderma koningii抑制B.cinerea的效果达94%～100%,其效果优于所测试的从仙客来上分离的真菌和细菌的多数菌株。用整株仙客来测试生防菌效果表明,C.rosea在叶片、花瓣、叶柄和花梗上抑制B.cinerea效果分别达96%、58%、53%、77%;而Trichoderma koningii的效果分别达100%、42%、100%、90%。以析因试验设计对B.cinerea和C.rosea或T.koningii施用浓度在生防效果上的交互效应进行了测定,结果表明:当C.rosea的接种浓度是病原菌浓度的10倍或10倍以上时,其在叶片、花瓣、叶柄和花梗上抑制B.cinerea的效果分别达73%～100%、52%～86%、100%、54%～68%;当T.koningii的接种浓度为106个/mL或以上时,其抑制B.cinerea在仙客来(接种浓度为103～106个/mL时)各部分发生的效果分别为:叶片100%、叶柄62%～100%、花瓣38%～96%、花梗56%～100%。C.rosea在叶龄为2、3、5、6周的叶片上的生防效果更好,而在叶龄为4周的叶片上效果差些,在叶龄为1周的叶片上无效果。T.koningii在叶龄为1～6周的叶片上均可有效地抑制B.cinerea的发生,抑制效果达89%～100%。C.rosea和T.koningii均是在温室内防治仙客来灰霉菌B.cinerea的具潜力的有效生防菌,但T.koningii的防治效果显著地优于C.rosea的效果。

关键词：拮抗微生物生物防治 Botrytis cinerea Clonostachys rosea 仙客来 Trichoderma koningii

Acute and Long-Term Treatments with an Herbal Formula V-Vital Capsule Increase Exercise Endurance Capacity in Weight-Loaded Swimming Mice

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Chinese Medicine 2014年第3期5卷 153-164页

作者： Pou Kuan Leong Hoi Yan Leung Wing Man Chan Ji Hang Chen Hoi Shan Wong Chung Wah Ma Shi Yu Zou Kam Ming Ko Division of Life Science The Hong Kong University of Science & Technology Hong Kong SAR China Infinitus (China) Company Ltd. Guangzhou China

Fatigue is a self-limiting response arising from physical and/or mental weariness, with a consequent personal and economic morbidity on work performance and social relationships. Anti-fatigue intervention is therefore urgently sought. “Qi-invigorating” Chinese tonic herbs, which can improve the energy status in the body according to the theory of traditional Chinese medicine, may produce beneficial effects in fatigue individuals. The herbal formula V-Vital capsule (VVC), which comprises 3 “Qi-invigorating” herbs, namely the root of Rhodiola rosea, Eleutherococcus senticosus and Panax quinquefolium, may produce anti-fatigue effect. In the present study, we investigated the effect of acute/long-term VVC treatment (acute: 0.75, 0.2 and 3.75 kg/day × 1 dose;long-term: 0.075 and 0.25 g/kg/day × 14 doses) on weight-loaded swimming female ICR mice. The weight-loaded swimming time until exhaustion, indicative of exercise endurance capacity, was recorded. Plasma levels of glucose, non-esterified fatty acid (NEFA), lactate and reactive oxygen metabolites (ROM) were measured in the exhausted mice. Glycogen levels in skeletal muscle and liver tissues were also measured. Mitochondrial function status [such as adenine nucleotide translocase (ANT) activity and coupling efficiency] was assayed. Results showed that acute VVC treatment increased the exercise endurance capacity in weight-loaded swimming mice. The ability of acute VVC treatment to enhance the exercise endurance was associated with increases in plasma glucose levels as well as glycogen levels in skeletal muscles and liver tissues, presumably due to the utilization of plasma lactate for gluconeogenesis and/or glycogen synthesis in the liver. While acute VVC treatment reduced the plasma ROM level in weight-loaded swimming mice, it increased the ANT activity. In this regard, the enhancement in exercise endurance afforded by acute VVC treatment might be due to an increase in the glucose supply to the skeletal muscle, the amelioration of systemic oxidative stress and the

关键词： Fatigue Exercise Endurance Rhodiola rosea Eleutherococcus senticosus Panax quinquefolium

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海参细菌的分离鉴定和生长特性研究

中国食品学报 2006年第1期6卷 25-29页

作者：向怡卉苏秀榕董明敏张聪林叶宁波大学生命科学与生物工程学院宁波315211

对大连产海参(刺参)不同部位的细菌进行了分离鉴定,得到3种菌株,分别为Kocuriaroseastacke-brandt、棒状杆菌(Corynebacteriumsp.)和有毒威克斯菌(WeeksellaviroseHolmes),其中Kocuriaroseastacke-brandt在国内尚未见报道,国内的菌种库中也没有。有毒威克斯菌是一种致病菌,其余两种为优势菌。对这3种菌进行了胞外酶活性研究,同时研究了温度、盐浓度和pH值对菌生长的影响。实验结果表明:3种菌对酯均无分解能力,对蛋白有一定的分解能力。棒状杆菌、Kocuriaroseastackebrandt和有毒威克斯菌最佳生长条件分别为:20℃,pH5.5,盐质量分数3%;25℃,pH5.5,盐质量分数4%;20℃,pH6.5,盐质量分数3.5%。

关键词：海参(刺参)Kocuria rosea stackebrandt 棒状杆菌有毒威克斯菌