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The RALF1-FERONIA Complex Phosphorylates eIF4E1 to Promote Protein Synthesis and Polar root Hair Growth

Molecular Plant 2020年第5期13卷 698-716页

作者： Sirui Zhu Jose Manuel Estevez Hongdong Liao Yonghua Zhu Tao Yang Chiyu Li Yichuan Wang Lan Li Xuanming Liu Javier Martinez Pacheco Hongwei Guo Feng Yu State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biologyand Hunan Key Laboratory of Plant Functional Genomics and Developmental RegulationHunan UniversityChangsha 410082P.R.China Fundacion Instituto Leloir Buenos Aires C1405BWEArgentina and IIBBA-CONICETBuenos Aires C1405BWEArgentina Centro de Biotecnologfa Vegetal Facultad de Ciencias de la VidaUniversidad Andres BelloSantiago RM 8370146Chile National Engineering Laboratory for Rice and Byproduct Deep Processing Central South University of Forestry and TechnologyChangsha 410004P.R.China Institute of Plant and Food Science Department of BiologySouthern University of Science and TechnologyShenzhenGuangdong 518055P.R.China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterChangsha 410125P.R.China

The molecular links between extracellular signals and the regulation of localized protein synthesis in plant cells are poorly understood.Here,we show that in Arabidopsis thaliana,the extracellular peptide RALF1 and its receptor,the FERONIA receptor kinase,promote root hair(RH)tip growth by modulating protein synthesis.We found that RALF1 promotes FERONIA-mediated phosphorylation of elF4E1,a eukaryotic translation initiation factor that plays a crucial role in the control of mRNA translation rate.Phosphorylated elF4E1 increases mRNA affinity and modulates mRNA translation and,thus,protein synthesis.The mRNAs targeted by the RALF1-FERONIA-elF4E1 module include ROP2 and RSL4,which are important regulators of RH cell polarity and growth.RALF1 and FERONIA are expressed in a polar manner in RHs,which facilitate elF4E1 polar丨ocalization and thus may control local f?OP2 translation.Moreover,we demonstrated that high-level accumulation of RSL4 exerts negative-feedback regulation of RALF1 expression by directly binding the RALF1 gene promoter,determining the final RH size.Our study reveals that the link between RALF1-FERONIA signaling and protein synthesis constitutes a novel component regulating cell expansion in these polar growing cells.

关键词： elF4E1 FER polar cell growth RALF1 root hairs RSL4

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The lncRNA APOLO interacts with the transcription factor WRKY42 to trigger root hair cell expansion in response to cold

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Molecular Plant 2021年第6期14卷 937-948页

作者： Michael Moison Javier Martinez Pacheco Leandro Lucero Camille Fonouni-Farde Johan Rodriguez-Melo Natanael Mansilla Aurelie Christ Jeremie Bazin Moussa Benhamed Fernando Ibanez Martin Crespi Jose M.Estevez Federico Ariel Instituto de Agrobiotecnología del Litoral Universidad Nacional del LitoralCONICETFBCB/FHUCCentro Científico Tecnológico CONICET Santa FeColectora Ruta Nacional No 168 km.0Paraje El PozoSanta Fe 3000Argentina Fundación Instituto Leloir and IIBBA-CONICET Av.Patricias Argentinas 435Buenos Aires CP C1405BWEArgentina Instituto de Investigaciones Agrobiotecnológicas CONICETUniversidad Nacional de Río CuartoRío Cuarto 5800Argentina Institute of Plant Sciences Paris-Saclay(IPS2) CNRSINRAUniversity Paris-Saclay and University of Paris Bâtiment 63091192 Gif sur YvetteFrance Centro de Biotecnología Vegetal(CBV) Facultad de Ciencias de la Vida(FCsV)Universidad Andres BelloSantiagoChile and Millennium Institute for Integrative Biology(iBio)SantiagoChile

Plant long noncoding RNAs(lncRNAs)have emerged as important regulators of chromatin dynamics,impacting on transcriptional programs leading to different developmental outputs.The lncRNA AUXIN-REGULATED PROMOTER LOOP(APOLO)directly recognizes multiple independent loci across the Arabidopsis genome and modulates their three-dimensional chromatin conformation,leading to transcriptional shifts.Here,we show that APOLO recognizes the locus encoding the root hair(RH)master regulator root HAIR DEFECTIVE 6(RHD6)and controls RHD6 transcriptional activity,leading to cold-enhanced RH elongation through the consequent activation of the transcription factor gene RHD6-like RSL4.Furthermore,we demonstrate that APOLO interacts with the transcription factor WRKY42 and modulates its binding to the RHD6 promoter.WRKY42 is required for the activation of RHD6 by low temperatures and WRKY42 deregulation impairs cold-induced RH expansion.Collectively,our results indicate that a novel ribonucleoprotein complex with APOLO and WRKY42 forms a regulatory hub to activate RHD6 by shaping its epigenetic environment and integrate signals governing RH growth and development.

关键词： root hairs long noncoding RNAs APOLO RHD6 WRKY42 cold temperature

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Complex Regulation of Prolyl-4-Hydroxylases Impacts root Hair Expansion

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Molecular Plant 2015年第5期8卷 734-746页

作者： Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen Petersen Norberto D. lusem Jose M. Estevez Instituto de Fisiologfa Biologia Molecular y Neurociencias (IFIBYNE-CONICET) Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires C1428EGA Argentina VKR Research Centre Department of Plant and Environmental Sciences University of Copenhagen DK-1871 Frededksberg C Denmark Joint BioEnergy Institute Feedstocks Division Lawrence Berkeley National Laboratory 5885 Hollis Street Emeryville CA 94608 USA Copenhagen Center for Glycomios Department of Cellular and Molecular Faculty of Health and Medical Sciences University of CopenhagenDK-2200 Copenhagen N Denmark Department of Botany University of Wisconsin Madison WI 53706 USA RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan Laboratory of Plant Nutrition Faculty of Agriculture Kyushu University Hakozaki 6-10-1 Higashi-ku Fukuoka 812-8581 Japan Departamento de Fisiologia Biologia Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires C1428EGAArgentina These authors contributed equally to the article. Present address: Fungal Physiology CBS-KNAW Fungal Biodiversity Center Uppsalalaan 8 Utrecht 3584 CT The Netherlands Present address: The Danish Stem Cell Center The Panum Institute Blegdamsvej 3B DK-2200 Copenhagen Denmark

root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae. However, structural plant cell walls impose constraints to accomplish tip growth. In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins （HRGPs）, which include several groups of O-glycoproteins, including extensins （EXTs）. Proline hydroxylation, an early post-translational modification （PTM） of HRGPs catalyzed by prolyl 4-hydroxylases （P4Hs）, defines their subsequent O-glycosylation sites. In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth. Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs. Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5. These three P4Hs are shown to be targeted to the secretory pathway, where P4H5 forms dimers with P4H2 and P4H13. Finally, we explore the impact of deficient proline hydroxylation on the cell wall architec- ture. Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.

关键词： enzymology cell expansion cell walls protein targeting proline hydroxylation root hairs

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Ubiquitin-Specific Protease 14 （UBP14） Is Involved in root Responses to Phosphate Deficiency in Arabidopsis

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Molecular Plant 2010年第1期3卷 212-223页

作者： Wen-Feng Li Paula J. Perry Nulu N. Prafulla Wolfgang Schmidt Institute of Plant and Microbial Biology Academia Sinica 115 Taipei

A mutant isolated from a screen of EMS-mutagenized Arabidopsis lines, per1, showed normal root hair development under control conditions but displayed an inhibited root hair elongation phenotype upon Pi deficiency. Additionally, the per1 mutant exhibited a pleiotropic phenotype under control conditions, resembling Pi-deficient plants in several aspects. Inhibition of root hair elongation upon growth on low Pi media was reverted by treatment with the Pi analog phosphite, suggesting that the mutant phenotype is not caused by a lack of Pi. Reciprocal grafting experiments revealed that the mutant rootstock is sufficient to cause the phenotype. Complementation analyses showed that the PER1 gene encodes an ubiquitin-specific protease, UBP14. The mutation caused a synonymous substitution in the 12th exon of this gene, resulting in a lower abundance of the UBP14 protein, probably as a consequence of reduced translation efficiency. Transcriptional profiling of per1 and wild-type plants subjected to short-term Pi starvation revealed genes that may be important for the signaling of Pi deficiency. We conclude that UBP14 function is crucial for adapting root development to the prevailing local availability of phosphate.

关键词： Phosphate deficiency root hairs ubiquitin-specific protease root development.

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Induction of root Hair Growth in a Phosphorus-Buffered Culture Solution

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Agricultural Sciences in China 2006年第5期5卷 370-376页

作者： LIU Guo-dong James Dunlop Thai Phung Institute of Natural Resources and Regional Planning Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China AgResearch Grasslands Research Center Private Bag 11008 Palmerston North New Zealand

A system to control the release of phosphate in water was successfully established, based on solubility product of [Ca^2＋] and [PO4^3-] using tricalcium phosphate as P source in the hydroponic solution, and adding CaCl2 for supplementing extra Ca^2＋. The system, similar to soil solutions, was a P nutrient buffer solution with very low bioavailable P. The buffer solution induced the roots of both monocotyledon and dicotyledon species to grow abundant root hairs, 3 mm in maximum length. The monocotyledons were corn （Zea mays L.）（var. Yellow Rose）, wheat （Triticum aestivum L.）（var. Yanzhong 144）, Triticale secale L. （vat. Jingsong 5）, and ryegrass （Lolium rigidum L.）（var. Ruanni）, and the dicotyledons were Arabidopsis thaliana L. （var. Columbia）, white clover （Trifolium repens）（var. Kopu）, Lotus （Lotus peduncucatus Cav. Luliginosus Schkuhr）（var. Grasslands Maku）. For these species we proved that the root environment controls the induction of root hair formation. However, the hydroponic buffer solution failed to induce root hairs on the roots of onion （Allium cepa L.）. Other investigators have concluded that corn does not form root hairs in hydroponics, but abundant long root hairs on corn were induced by this buffer system. The roots with abundant long root hairs are called ＂hedgehog roots＂ because they have hairs everywhere just like a hedgehog.

关键词： low-P stress root hairs Arabidopsis thaliana Zea mays Triticum aestivum

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