检索结果-南通市图书馆

DISSOCIATION AND RECONSTITUTION OF IMMOBILIZED ribulose-1, 5-BISPHOS PHATE CARBOXYLASE//OXYGENASE FROM TOBACCO

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1988年第10期31卷 1204-1212页

作者：李立人李粹芳许仁邦 Shanghai Institute of Plant Physiology Academia Sinica

ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco covalently coupled to CNBr-activated Sepharose 4B was treated with urea. Analysis by electrophoresis showed that the small subunit was dissociated at 2—2.5 mol/L urea, while the large subunit was still bound to matrix. The large subunit core, L, was further dissociated into monomer at 3 mol/L urea. It is suggested that RuBPCase is coupled to Sepharose by virtue of ε-NHon a large subunit. The activity of the immobilized enzyme was inversely proportional to the amount of small subunit dissociated by urea. The dissociated small subunits were almost completely bound back to the S-depleted immobilized RuBPCase, if the urea concentration was diluted to 0.5 mol/L. The enzyme activity could be recovered nearly to 100％. The activity of the S-depleted enzyme was linearly correlated on the concentration of small subunits in solution. These results indicate that the small subunit plays an important role in the maintenance of RuBPCase activity.

关键词： tobacco ribulose-1 5-bisphosphate carboxylase/oxygenase immobilization dissoeiation reconstitution.

IMMOBILIZATION AND SUBUNIT RECONSTITUTION OF ribulose-1, 5-BISPHOSPHATE CARBOXYLASE/OXYGENASE FROM RICE

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1991年第14期36卷 1212-1216页

作者：李立人许仁邦缪有刚 Shanghai Institute of Plant Physiology Academia Sinica Shanghai 200032 PRC

ribulose-1, 5-bisphosphate carboxylase/oxygenase ( EC 4.***.1.39 ) ( RuBP carboxylase ) is a bifunctional enzyme which catalyzes both carboxylation and oxygenation of ribulose-1, 5-bisphosphate (RuBP). These reactions are the first step in photosynthetic carbon fixation and photorespiration respectively. Because of its key role in the formation of plant yield, many studies on the structure of active-site and catalytic mechanism of this enzyme have

关键词： rice ribulose-1 5-bisphosphate carboxylase/oxygenase reconstitution.

Distribution characteristics and diversities of cbb and coxL genes in paddy soil profiles from southern China

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2021年第6期31卷 954-963页

作者： Wentao PENG Yan WANG Xiuxiu ZHU Liufeng XU Juan ZHAO Zhongli CUI Hui CAO College of Life Sciences/Key Laboratory of Agricultural Environmental Microbiology Ministry of Agriculture and Rural AffairsNanjing Agricultural UniversityNanjing 210095(China) Rubber Research Institute Chinese Academy of Tropical Agricultural SciencesHaikou 571101(China)

The Calvin-Benson-Bassham cycle and Wood-Ljungdahl pathway are two well-known ones in the six carbon sequestration pathways,but the current knowledge of their occurrence in different layers of agricultural soil profiles is *** this study,the diversities of three genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubis CO),i.e.,genes encoding the green-like(cbbLG)and red-like(cbbLR)forms of Rubis COⅠ and encoding Rubis COⅡ(cbbM),and the gene encoding carbon monoxide dehydrogenase large subunit(coxL)from five paddy soils in southern China were investigated by real-time quantitative polymerase chain reaction,restriction fragment length polymorphism(RFLP)analysis,and clone *** abundances of the four genes ranged from 10^(7) to 10^(9) copies g^(-1) soil,and the cbbLR gene outnumbered the other three genes in all soil samples,suggesting important roles they play in carbon dioxide(CO_(2))*** addition,it was found that the copy numbers of cbbLR and cbbLG decreased with increasing soil depth,while the copy numbers of cbbM and coxL decreased in the shallow depths but increased with increasing soil *** results of RFLP showed a larger Shannon index(H)in the deeper soil layers among the four gene clone libraries,indicating that the community diversity in these soil layers was *** cbbLG gene had relatively low diversity(at genus level),and most of the sequences were classified as Sideroxydans and *** contrast,the highly diverse groups were found in the other three gene clone libraries(cbbLR,cbbM,and coxL),most of which were distantly related to known sequences,even forming separate *** summary,this study provides a new insight into CO_(2) fixers along agricultural soil profiles by comparing four bacterial genes.

关键词： Calvin-Benson-Bassham cycle carbon dioxide fixation carbon monoxide dehydrogenase ribulose-1 5-bisphosphate carboxylase/oxygenase

Cloning and Sequence Analysis of rbcS Gene of Wild Barley (Hordeum brevisubulatum) under Salt Stress

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2010年第8期11卷 42-44页

作者：岳海燕尹剑锐闫守庆冯宇隆张莲姬郭建强李怀亮丁雪梅沈景林吉林大学畜牧兽医学院吉林长春130062

[Objective] The aim was to study the cloning and sequence analysis of rbcS gene of wild barley under salt stress. [Method] The tender leaf blade of wild barley under salt stress was taken as the experimental material. The primers were designed according to the homology of rbcS gene sequences of wheat and barely in Genbank; then PCR amplification,recovery,ligation,transformation and sequencing of rbcS gene were carried out. [Result] Two rbcS genes including rbcS1 and rbcS2 with the length of 1 252 and 908 bp respectively were cloned from the barely genome. rbcS1 and rbcS2 were both composed by two exons and one intron. The exons length of the two genes was the same of 525 bp,encoding 174 amino acids,and the homology between them was 96%; however,the intron length of rbcS1 and rbcS2 was 448 and 107 bp respectively.

关键词： Wild barley Salt stress ribulose-1 5-Bisphosphate Carboxylase/Oxygenase Small Subunit Sequence analysis