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Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

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Journal of Animal Science and Biotechnology 2020年第1期11卷 44-57页

作者： MariaPortia B.Nagata Junki Egashira Naoto Katafuchi Kenji Endo Kazuko Ogata Kenichi Yamanaka Tadayuki Yamanouchi Hideo Matsuda Yutaka Hashiyada Kenichi Yamashita Advanced Manufacturing Research Institute National Institute of Advanced Industrial Science and Technology(AIST)807-1 Shuku-machiTosuSaga 841-0052Japan Saga Prefectural Livestock Experiment Station 23242-2 Yamauchi-machiMiyanoTakeoSaga 849-2305Japan Morinaga Dairy Service Co.Ltd. 1-159 ToyoharaotsuNasu-gun Nasu-machiTochigi 329-3224Japan National Livestock Breeding Center(NLBC) 1 OdakuraharaOdakuraNishigo-muraNishishirakawa-gunFukushima 961-8511Japan Institute of Livestock and Grassland Science National Agriculture and Food Research Organization(NARO)Ikenodai 2TsukubaIbaraki 305-0901Japan Faculty of Agriculture Saga University1 Honjo-machiSaga 840-8502Japan.7Ishikawa Prefectural University1-308 SuematsuNonoichi-shiIshikawa 921-8836Japan.

Background:The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal ***,a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation,rendering the frozen-thawed semen impractical for routine *** is known to reduce sperm lifespan and *** in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination(AI)*** goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation ***:Our strategy of sperm selection based on rheotaxis and thermotaxis(SSRT)on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity,viability and biological function related to fertilization *** strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat *** technique protected further sublethal damage to bovine spermatozoa(higher%cryosurvival than the control)and resulted in the improvement of DNA *** selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo ***:These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival,longevity of post-thawed sperm,and optimization of in vivo fertilization,embryo development and calving as supported by the favorable results of field fertility study.

关键词： Cryopreservation Fertility Livestock rheotaxis Spermatozoa Thermotaxis

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Behavioral methods for the functional assessment of hair cells in zebrafish

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Frontiers of Medicine 2017年第2期11卷 178-190页

作者： Qin Yang Peng sun Shi Chen Hongzhe Li Fangyi Chen Department of Biology South University of Science and Technology of China Shenzhen 518000 China Department of Basic Medicine Wuhan University Wuhan 430000 China State Key Laboratory of Analog and Mixed-Signal VLSI University of Macao Macao China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education School of Pharmaceutical Sciences and Medical Research Institute Wuhan University Wuhan 430072 China Research Service VA Loma Linda Healthcare System Loma Linda CA 92357 USA Department of Otolaryngology - Head & Neck Surgery Loma Linda University School of Medicine Loma Linda CA 92350 USA

Zebrafish an emerging animal model for studies comparability with humans, good accessibility to the hearing utilize this animal model, methodologies need to be used to on auditory system. This model presents high organ, and high throughput capacity. To better quantify the hearing function of the zebrafish. Zebrafish displays a series of innate and robust behavior related to its auditory function. Here, we reviewed the advantage of using zebrafish in auditory research and then introduced three behavioral tests, as follows： the startle response, the vestibular-ocular reflex, and rheotaxis. These tests are discussed in terms of their physiological characteristics, up-to-date technical development, and apparatus description. Test limitation and areas to improve are also introduced. Finally, we revealed the feasibility of these applications in zebrafish behavioral assessment and their potential in the high-throughput screening on hearing-related genes and drugs.

关键词： zebrafish (Danio rerio) behavior auditory startle response vestibular-ocular reflex rheotaxis

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Behavioral mechanisms of mammalian sperm guidance

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Asian Journal of Andrology 2015年第4期17卷 628-632页

作者： Serafin Perez-Cerezales Sergii Boryshpolets Michael Eisenbach Department of Biological Chemistry The Weizmann Institute of Science 7610001 Rehovot Israel.

In mammals, sperm guidance in the oviduct appears essential for successful sperm arrival at the oocyte. Hitherto, three different potential sperm guidance mechanisms have been recognized： thermotaxis, rheotaxis, and chemotaxis, each of them using specific stimuli - a temperature gradient, fluid flow, and a chemoattractant gradient, respectively. Here, we review sperm behavioral in these mechanisms and indicate commonalities and differences between them.

关键词： chemotaxis rheotaxis sperm behavior sperm motility thermotaxis

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