检索结果-南通市图书馆

Expression Profiling of Cassava Storage Roots Reveals an Active Process of Glycolysis/Gluconeogenesis

Journal of Integrative Plant Biology 2011年第3期53卷 193-211页

作者： Jun Yang Dong An Peng Zhang Shanghai Center for Cassava Biotechnology National Laboratory of Plant Molecular Genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Key Laboratory of Synthetic Biology Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Shanghai Chenshan Plant Science Research Center the Chinese Academy of Sciences Chenshan Botanical Garden Shanghai 201602 China

Mechanisms related to the development of cassava storage roots and starch accumulation remain largely unknown. To evaluate genome-wide expression patterns during tuberization, a 60 mer oligonucleotide microarray representing 20 840 cassawl genes was designed to identify differentially expressed transcripts in fibrous roots, developing storage roots and mature storage roots. Using a random variance model and the traditional twofold change method for statistical analysis, 912 and 3 386 upregulated and downregulated genes related to the three developmental phases were identified. Among 25 significantly changed pathways identified, glycolysis/gluconeogenesis was the most evident one. Rate-limiting enzymes were identified from each individual pathway, for example, enolase, L-lactate dehydrogenase and aldehyde dehydrogenase for glycolysis/gluconeogenesis, and ADP-glucose pyrophosphorylase, starch branching enzyme and glucan phosphorylase for sucrose and starch metabolism. This study revealed that dynamic changes in at least 16% of the total transcripts, including transcription fac- tors, oxidoreductases/transferases/hydrolases, hormone-related genes, and effectors of homeostasis. The reliability of these differentially expressed genes was verified by quantitative real-time reverse transcription-polymerase chain reaction. Tlhese studies should facilitate our understanding of the storage root formation and cassava improvement.

关键词： CASSAVA ROOTS (Botany) -- Formation GLYCOLYSIS GLUCONEOGENESIS LACTATE dehydrogenase reverse transcriptase polymerase chain reaction PLANT gene expression OLIGONUCLEOTIDE arrays

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a real-time PCR method for the detection of bacterial colonization in rat models of severe acute pancreatitis

引用

Chinese Medical Journal 2010年第3期123卷 326-331页

作者： PENG Jun-sheng LIU Zhong-hui LI Chu-jun WU Xiao-bin DIAO De-chang DU Yan-ping CHEN Jun-rong LI Yun WANG Hua-she Department of Gastrointestinal Surgery Department of Gastroenterology

Background Techniques for the fast and accurate detection of bacterial infection are critical for early diagnosis, prevention and treatment of bacterial translocation in clinical severe acute pancreatitis （SAP）. In this study, the availability of a real-time PCR method in detection of bacterial colonization in SAP rat models was investigated. Methods Samples of blood, mesenteric lymph nodes （MLN）, pancreas and liver from 24 specific pathogen-free rats （8 in a control group, 16 in a SAP group） were detected for bacterial infection rates both by agar plate culture and a real-time PCR method, and the results were made contrast. Results Bacterial infection rates of the blood, MLN, pancreas and liver in the SAP group and the control group by the two different methods were almost the same, which were 5/16, 12/16, 15/16, 12/16 in the SAP group compared with 0/8, 1/8, 0/8, 0/8 in the control group by agar plate culture, while 5/16, 10/16, 13/16, 12/16 and 0/8, 1/8, 0/8, 0/8 respectively by a real-time PCR method. Bacterial number was estimated by real-time PCR, which showed that in the same mass of tissues, the pancreas contained more bacteria than the other three kinds of organs in SAP rats （P 〈0.01）, that may be due to the edema, necrosis and hemorrhage existing in the pancreas, making it easier for bacteria to invade and breed. Conclusion Fast and accurate detection of bacterial translocation in SAP rat models could be carried out by a real-time PCR procedure.

关键词： enumeration infection pancreatitis bacillus reverse transcriptase polymerase chain reaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two novel mutations of the LDL receptor gene associated with familial hypercholesterolemia in a Chinese family

引用

Chinese Medical Journal 2007年第19期120卷 1694-1699页

作者： XIE Li GONG Qi-hua XIE Zhi-guo LIANG Zong-min HU Zheng-mao XIA Kun XIA Jia-hui YANG Yi-feng Department of Cardiothoracic Surgery Second Xiangya Hospital of Central South University Changsha 410011 China National Laboratory of Medical Genetics of China Changsha 410011 China

Background Familial hypercholesterolemia （FH） is a type of dominant autosomal disease that causes high levels of plasma low-density lipoprotein cholesterol （LDL-C）. In the past years, molecular data related to FH were limited in China. Now, to gain more information about FH, we analyzed one proband with a severe FH phenotype as well as his relatives. Methods After the entire coding sequence and the intron-exon junctions of the low-density lipoprotein receptor （LDLR） gene were amplified using PCR, we sequenced the LDLR gene of a Chinese FH family. RT-PCR was used to detect changes in the mRNA. Results Two novel mutations were identified in the LDLR gene of this family. One, W165X, was a G〉A substitution at the third nucleotide of codon 165. The other, IVS5-1G〉A, was also a G〉A substitution at the acceptor splice site of intron 5. The most striking discovery is that the proband was heterozygous for W165X but homozygous for IVS5-1G〉A. The cDNA sequencing showed that the IVS5-1G〉A mutation caused the insertion of 10 nucleotides, namely GCTCTCACAA, between exon 5 and exon 6. Conclusions The two nucleotide variations are thought to be the FH-causing mutations because the co-segregation of the mutant allele with the phenotype of FH has been shown in this Chinese family. These data show an increase in the mutational spectrum of FH in China and verify a scarce mutational form in the LDLR gene.

关键词： familial hypercholesterolemia low-density lipoprotein reverse transcriptase polymerase chain reaction mutation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression of nicotinic acetylcholine receptor subunit α9 in type Ⅱ vesti-bular hair cells of rats

引用

Acta Pharmacologica Sinica 2006年第11期27卷 1509-1514页

作者： Wei-jia KONG~(2,4,5)Hua-mao CHENG~(2,4)Paul van CAUWENBERGE~3 2 Department of Otorhinolaryngology,Union Hospital of Tongji Medical College,Huazhong Science and Technology University,Wuhan 430030,China 3 Department of Otorhinolaryngology,Ghent University Hospital,De Pintelaan 185,9000 Gent,Belgium DepartmentofOtorhinolaryngology UnionHospitalofTongjiMedicalCollegeHuazhongScienceandTechnologyUniversityWuhan430030China DepartmentofOtorhinolaryngology GhentUniversityHospitalDePintelaan1859000GentBelgium

Aim:To explore the cell specific existence of α9 AChR in the vestibular type Ⅱ haircells(VHC Ⅱ)of rats.Methods:To detect the expression of α9 AChR messengerRNA(mRNA)in the vestibular endorgans and single VHC Ⅱ of rats by using thereverse transcription polymerase chain reaction(RT-PCR)technique and the singlecell RT-PCR technique,respectively.Results:It was shown that α9 AChR mRNAwas detected in the vestibular endorgans.By using single-cell RT-PCR,mRNAencoding α9 AChR was also detected in the VHC Ⅱ of the rats.Sequence analysisof the PCR products confirmed identity to corresponding cDNA sequence in thepredicted region.Conclusion:We established a method which could effectivelydetect the cell specific expression of mRNA in an individual VHC.Present dataconfirm that α9 AChR mRNA is expressed in the VHC Ⅱ of rats and indicates thatα9 AChR may function as a mediator of efferent cholinergic signaling in mamma-lian VHC.

关键词： hair cells vestibule rats nicotinic acetylcholine receptor single cell reverse transcriptase polymerase chain reaction

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effects of simvastatin on cardiac performance and expression of sarcoplasmic reticular calcium regulatory proteins in rat heart

引用

Acta Pharmacologica Sinica 2005年第6期26卷 696-704页

作者： Xia ZHENG, Shen-jiang HU~2 The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China TheFirstAffiliatedHospital CollegeofMedicineZhejiangUniversityHangzhou310003China

Aim: To investigate the effect of simvastatin on the cardiac contractile function and the alteration of gene and protein expression of the sarcoplasmic calcium regulatory proteins, including sarcoplasmic reticulum Ca2+-ATPase (SERCA), phospholamban (PLB), and ryanodine receptor 2 (RyR2) in rat hearts. Methods: Langendorff-perfused rat hearts were subjected to 60-min perfusion with different concentrations of simvastatin (1,3,10, 30, or 100 μmol/L), and the parameters of cardiac function such as left ventricular developed pressure (LVDP), +dP/dtmax, and -dp/dtmaxwere determined. The cultured neonatal rat ventricular cardiomyocytes were incubated with simvastatin (1,3,10,30, and 100 μmol/L) for 1 h or 24 h. The levels of SERCA, PLB, and RyR2 expression were measured by reverse tran-scription-polymerase chain reaction and Western blot. Cytotoxic effect of simvastatin on ventricular cardiomyocytes was assessed by the MTT colorimetric assay. Results: LVDP, +dp/dtmaxand -dp/dtmaxof hearts were increased significantly after treatment with simvastatin 3, 10, and 30 μmol/L. In simvastatin-treated isolated hearts, the levels of mRNA expression of SERCA and RyR2 were elevated compared with the control (P<0.05), while the mRNA expression of PLB did not change. After the cultured neonatal rat ventricular cardiomyocytes were incubated with 3,10,30, and 100 μmol/L simvastatin for 1 h, SERCA and RyR2 mRNA expressions of cardiomyocytes rose, but there was no alteration in protein expressions. However, with the elongation of simvastatin treatment to 24 h, the protein expression of SERCA and RyR2 were also elevated. Additionally, simvastatin (1-30 μmol/L) had no influence on cell viability of cultured cardiac myocytes, but simvastatin 100 μmol/L inhibited the cell viability. Conclusion: Simvastatin improved cardiac performance accompanied by the elevation of SERCA and RyR2 gene and protein expression.

关键词： simvastatin Ca2+-transporting ATPase endoplasmic reticulum calcium regulatory protein myocardial contraction ryanodine receptor calcium release channel reverse transcriptase polymerase chain reaction

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Effect of intestinal ischemia/reperfusion injury on leptin and orexin-A levels

引用

Frontiers of Medicine 2007年第1期1卷 87-92页

作者： LIN Ji YAN Guangtao GAO Xiaoning LIAO Jie HAO Xiuhua ZHANG Kai Research Laboratory of Biochemistry General Hospital of PLABeijing 100853China Department of Pediatrics General Hospital of PLABeijing 100853China Medical and Experimental Analysis Center General Hospital of PLABeijing 100853China

The aim of this paper is to explore the effect of intestinal ischemia/reperfusion(I/R)injury on leptin and orexin-A levels in peripheral blood and central secretory tissues,and to examine the roles of leptin and orexin-A in acute inflammatory responses.An intestinal I/R injury model of rats was made;the rats were grouped according to the time of after 60 min ischemia.Radioimmunoassay was employed to detect the levels of leptin in serum and adipose tissue and orexin-A levels in plasma and hypothalamus.reverse transcriptase-polymerase chain reaction was used to detect mRNA expressions of adipose leptin and hypothalamus orexin-A.Compared with the levels before the injury,serum leptin in 60 min ischemia/30 min reperfusion(I60pR30p)group decreased and that of I60pR360p group increased.Compared with sham-operation group(sham group)after injury,serum leptin level of I60pR360p group increased,adipose leptin levels of I60pR30p and I60pR90p decreased,and adipose leptin in I60pR360p group increased.After the injury,adipose leptin mRNA expressions of I60pR30p,I60pR240p and I60pR360p increased,whereas that of I60pR150p group decreased as compared with the sham group.There was no significant difference in the protein levels of orexin-A,either between plasma and hypothalamus or between pre-and post-I/R injury.Compared with sham group,hypothalamus orexin-A mRNA expressions of I60pR30p and I60pR90p decreased gradually after the injury,with that of I60pR150p group reaching the lowest,and those of I60pR240p and I60pR360p recovering gradually,although they were still significantly lower than that of sham group.Leptin and orexin-A respond to intestinal I/R injury in a time-dependent manner,with leptin responding more quickly than orexin-A does,and both of them may contribute to the metabolic disorders in acute inflammation.

关键词： reperfusion injury intestine leptin orexin-A radioimmunoassay reverse transcriptase polymerase chain reaction adipose tissue hypothalamus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Increase of β1-adrenergic receptor gene expression induced by nicotine in hippocampal slice of rat

引用

Acta Pharmacologica Sinica 2003年第12期24卷 107-112页

作者： WANG Yong, ZHU Xiao-Nan, YAN Jie YU Jian-Ping, HUANG Xiao-Hui, CHEN Ru-Zhu Department of Pharmacology, Sun Yat-sen University of Medical Science, Guangzhou 510089, China Department of Pharmacology Sun Yat-sen University of Medical Science Guangzhou 510089

AIM: To investigate the effect of nicotine on β1-adrenergic receptor (β1-AR) in the hippocampal slice of rat. METHODS: Hippocampal slices (400 μm thick) were incubated in artificial cerebrospinal fluid (ACSF) previously saturated with 95％ O2and 5％ CO2at 28℃ for 120 min, and then incubated with nicotine 10 μmol/L for 30, 60, 90, and 120 min. mRNA of the β1-adrenergic receptor was examined with semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), and the protein level was measured by Western blot and RIA. RESULTS: The mRNA gene expression and the protein level of β1-adrenergic receptor in hippocampal slices were increased after nicotine treatment. The peak of protein occurred later but higher than that of mRNA level. CONCLUSION: Both expression of β1-adrenergic receptor gene transcription and post-transcriptional protein level in rat hippocampus were altered by nicotine.

关键词： nicotine β-adrenergic receptors hippocampus reverse transcriptase polymerase chain reaction Western blotting radioligand assay

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Significance of peritoneal lavage cytology based on genetic signatures in gastric cancer

引用

Journal of Cancer Metastasis and Treatment 2018年第1期4卷 75-87页

作者： Tasuku Matsuoka Masakazu Yashiro Department of Surgical Oncology Osaka City University Graduate School of Medic ineOsaka 545-8585Japan Molecular Oncology and Therapeutics Osaka City University Graduate School of MedicineOsaka 545-8585Japan

Perit on eal metastasis is the most comm on patter n of recurre nee and the most freque nt cause of death after surgery in patie nts with gastric can cer.Perit on eal free can cer cells dissem in ated from the primary lesi on site have bee n con sidered the main cause of perit on eal metastasis.Perit on eal lavage cytological exam in ati on(PLC)has bee n show n to be an in depe ndent predictor of gastric cancer relapse after curative resection and poor overall survival.However,the conventional cytological examinations have high rates of false-positive and false-negative findings.To improve the sensitivity,molecular-based methods using reverse transcriptase polymerase chain reaction have been developed for detecting cancer cells in peritoneal wash fluids of patients with gastric cancer.We performed a PubMed search for articles describing PLC in gastric can cer.Releva nt articles were reviewed and data on available outcomes elaborated.The cli nical roles and attributes of PLC in gastric can cer were reviewed,and its future applicati on to this disease is discussed.

关键词： Gastric cancer peritoneal lavage cytology genetic detection reverse transcriptase polymerase chain reaction carcinoembryonic antigen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current status quo on COVID-19 including chest imaging

引用

World Journal of Radiology 2020年第12期12卷 272-288页

作者： Rishi Philip Mathew Merin Jose Vinayak Jayaram Paul Joy Danny George Maria Joseph TeenaSleeba Ajith Toms Department of Radiology Rajagiri HospitalKochi 683112KeralaIndia

With each day the number coronavirus disease 2019(COVID-19)cases continue to rise rapidly and our imaging knowledge of this disease is expeditiously evolving.The role of chest computed tomography(CT)in the screening or diagnosis of COVID-19 remains the subject of much debate.Despite several months having passed since identifying the disease,and numerous studies related to it,controversy and concern still exists regarding the widespread use of chest CT in the evaluation and management of COVID-19 suspect patients.Several institutes and organizations around the world have released guidelines,recommendations and statements against the use of CT for diagnosing or screening COVID-19 infection and advocating its use only for those cases with a strong clinical suspicion of complication or an alternate diagnosis.However,these guidelines and recommendations are in disagreement with majority of the widely available literature,which strongly favour CT as a pivotal tool in the early diagnosis,management and even follow-up of COVID-19 infection.This article besides comprehensively reviewing the current status quo on COVID-19 disease in general,also writes upon the current consensus statements/recommendations on the use of diagnostic imaging in COVID-19 as well as highlighting the precautions and various disinfection procedures being employed world-wide at the workplace to prevent the spread of infection.

关键词： COVID-19 SARS-CoV-2 Coronavirus disease reverse transcriptase polymerase chain reaction Viral pneumonia Computed tomography

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gene-CYP11B2 expression in rat liver in hepatic fibrogenesis induced by CCl_4

引用

Chinese Medical Journal 2001年第1期114卷 64-68页

作者：李旭杨希山吴平生孟莹李淑梅赖文岩 PLA Institute for Digestive Diseases Nanfang Hospital First Medical University of PLA Guangzhou 510515 China

identify aldosterone synthase gene CYP11B2 mRNA expression in normal and fibrotic liver in rats and evaluate the curative effect of antisterone Methods 160 Wistar rats weighing about 250?g were divided into 4 groups In the model group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week In the antisterone group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Antisterone equivalent to 20?mg·kg 1 ·d 1 was given intragastrically (ig) In the malotilate group (n=40), the rats were injected with 40% CCl 4 (0 25?ml/100?g) subcutaneously three times a week Malotilate equivalent to 50?mg·kg 1 ·d 1 was given ig In the control group (n=40), the rats were injected with olive oil only After 2,4,6,8 and 10 weeks, animals were sacrificed, and morphological examination was carried out The area of collagen was examined with an Image Analyse System Expression of the aldosterone synthase gene, CYP11B2 mRNA, in fibrotic and normal liver was detected by means of reverse transcriptase polymerase chain reaction (RT PCR) and in situ hybridization Results In situ hybridization and RT PCR showed that the expression of CYP11B2 mRNA, which localized in the endoplasm of hepatic stellate cells (HSCs), was up regulated when fibrogenesis occurred Histological observation indicated that the grade of fibrosis and the area of collagen in the antisterone group were less than those in model group before 6 weeks ( P 0 05) After that, however, the grade of fibrosis and the area of collagen in the antisterone group were higher than those in the malotilate group ( P 0 05) Conclusions The expression of CYP11B2 mRNA is up regulated in fibrotic liver Antisterone can have a partial fibrogenesis inh

关键词： CYP11B2 gene hepatic fibrogenesis hepatic stellate cells antisterone in situ hybridization reverse transcriptase polymerase chain reaction

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：