检索结果-南通市图书馆

Molting process revealed by the detailed expression profiles of RxR1/RxR2 and mining the associated genes in a spider mite, Panonychus citri

引用

Insect Science 2022年第2期29卷 430-442页

作者： Gang Li xun-Yan Liu Guy Smagghe Jin-Zhi Niu Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionInternational Joint Laboratory of China-Belgium on Sustainable Crop Pest ControlAcademy of Agricultural SciencesSouthwest UniversityChongqingChina Provincial Key Laboratory for Agricultural Pest Management of Mountainous Regions Institute of EntomologyGuizhou UniversityGuiyangChina Department of Plants and Crops Faculty of Bioscience EngineeringGhent UniversityGhentBelgium

Spider mites have one ecdysone receptor(EcR)and multiple retinoid X receptors(RxRs).However,the function of these RxRs in spider mite development is unknown.Here,we screened the expression dynamics of two PcRxR isoforms at 4 h intervals in the deutonymphal stage of Panonychus citri.The results showed that PcEcR had an expression pattern similar to that of PcRxR2.For PcRxR1,its expression remained at a certain high level,when there was a decrease of both PcEcR and PcRxR2.In situ hybridization showed that PcRxR2 was detected in the central nervous mass,while the ecdysteroid biosynthesis gene PcSpo was mainly expressed at the edge of the central nervous mass.RNAi-based silencing of PcRxR1 or PcRxR2 showed the same phenotype as in mites with that of silencing PcEcR.Furthermore,RNA-seq was used to mine the genes associated with the expression dynamics of PcRxR1 or PcRxR2,which revealed that the heterodimer of EcR-RxR2 in spider mites might be linked with the cell autophagy and tissue remodeling during apolysis,and RxR1 might be linked with new epicuticle and exocuticle secretion during ecdysis.Taken together,these results increase our understanding of the regulation mechanism of ecdysteroid signal pathway in spider mite development.

关键词： ecdysone receptor(EcR) in situ hybridization retinoid X receptors RNA interference RNA-seq

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：