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Nano Research 2021年第11期14卷 3840-3847页

作者： Miaomiao Tang Lu Zhang Bin Song Xiaoyuan Ji Chenyu Wang Houyu Wang Hua Xu Yuanyuan Su Yao He Laboratory of Nanoscale Biochemical Analysis Institute of Functional Nano and Soft Materials(FUNSOM)and Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow UniversitySuzhou215123China Department of Ophthalmology Children’s Hospital of Soochow UniversitySoochow UniversitySuzhou215123China Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Medical Research CenterSun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou510120China

Fluorescein angiography(FA)is a standard imaging modality for evaluating vascular abnormalities in retina-related diseases,which is recognized as the major cause of vision ***-term and real-time fundus angiography is of great importance in preclinical research,nevertheless remaining big challenges up to *** this study,we demonstrate that long-term fluorescence imaging of retinal vessels is enabled through a kind of fluorescent nanoagents,which is made of small-sized(hydrodynamic diameter:∼3 nm)silicon nanoparticles(SiNPs)featuring strong fluorescence,robust photostability,lengthened blood residency and negligible *** particular,the presented SiNPs-based nanoagents are capable of imaging retinal capillaries in∼10 min,which is around 10-fold longer than that(∼1 min)of fluorescein sodium(FS,known as the most widely used contrast agents for FA in clinic).Taking cynomolgus macaques as non-human primate-animal model,we further demonstrate the feasibility of real-time diagnosis of retinal diseases(e.g.,age-related macular degeneration(AMD))through dynamic monitoring of vascular dysfunction.

关键词： fluorescent silicon nanoparticles long-term real-time bioimaging retinal diseases

Convolutional Neural Network-Based Classificationof Multiple retinal diseases Using Fundus Images

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Intelligent Automation & Soft Computing 2023年第6期36卷 2607-2622页

作者： Aqsa Aslam Saima Farhan Momina Abdul Khaliq Fatima Anjum Ayesha Afzaal Faria Kanwal Lahore College for Women University Lahore54000Pakistan

Use of deep learning algorithms for the investigation and analysis of medical images has emerged as a powerful *** increase in retinal dis-eases is alarming as it may lead to permanent blindness if left ***-tion of the diagnosis process of retinal diseases not only assists ophthalmologists in correct decision-making but saves time *** researchers have worked on automated retinal disease classification but restricted either to hand-crafted fea-ture selection or binary *** paper presents a deep learning-based approach for the automated classification of multiple retinal diseases using fundus *** this research,the data has been collected and combined from three distinct *** images are preprocessed for enhancing the *** layers of the convolutional neural network(CNN)are used for the automated feature extraction and classification of 20 retinal *** is observed that the results are reliant on the number of *** binary classification(healthy ***),up to 100%accuracy has been *** 16 classes are used(treating stages of a disease as a single class),93.3%accuracy,92%sensitivity and 93%specificity have been obtained *** 20 classes(treating stages of the disease as separate classes),the accuracy,sensitivity and specificity have dropped to 92.4%,92%and 92%respectively.

关键词： Classification convolutional neural network fundus images medical image diagnosis retinal diseases

Intravitreal stem cell paracrine properties as a potential neuroprotective therapy for retinal photoreceptor neurodegenerative diseases

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Neural Regeneration Research 2020年第9期15卷 1631-1638页

作者： Kevin Puertas-Neyra Ricardo Usategui-Martín Rosa MCoco Ivan Fernandez-Bueno Instituto Universitario de Oftalmobiologia Aplicada Universidad de ValladolidValladolidSpain Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leon ValladolidSpain Red Tematica de Investigacion Cooperativa en Salud OftaredInstituto de Salud CarlosⅢValladolidSpain

retinal degenerations are the leading causes of irreversible visual loss worldwide. Many pathologies included under this umbrella involve progressive degeneration and ultimate loss of the photoreceptor cells, with age-related macular degeneration and inherited and ischemic retinal diseases the most relevant. These diseases greatly impact patients' daily lives, with accompanying marked social and economic consequences. However, the currently available treatments only delay the onset or slow progression of visual impairment, and there are no cures for these photoreceptor diseases. Therefore, new therapeutic strategies are being investigated, such as gene therapy, optogenetics, cell replacement, or cell-based neuroprotection. Specifically, stem cells can secrete neurotrophic, immunomodulatory, and anti-angiogenic factors that potentially protect and preserve retinal cells from neurodegeneration. Further, neuroprotection can be used in different types of retinal degenerative diseases and at different disease stages, unlike other potential therapies. This review summarizes stem cell-based paracrine neuroprotective strategies for photoreceptor degeneration, which are under study in clinical trials, and the latest preclinical studies. Effective retinal neuroprotection could be the next frontier in photoreceptor diseases, and the development of novel neuroprotective strategies will address the unmet therapeutic needs.

关键词： clinical trials growth factors intraocular injection intravitreal injection neuroprotection paracrine properties photoreceptors preclinical models retinal diseases stem cells

Pyrroloquinoline quinone:a potential neuroprotective compound for neurodegenerative diseases targeting metabolism

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Neural Regeneration Research 2025年第1期20卷 41-53页

作者： Alessio Canovai Pete A.Williams Department of Biology University of PisaPisaItaly Department of Clinical Neuroscience Division of Eye and VisionSt.Erik Eye HospitalKarolinska InstitutetStockholmSweden

Pyrroloquinoline quinone is a quinone described as a cofactor for many bacterial dehydrogenases and is reported to exert an effect on metabolism in mammalian cells/*** quinone is present in the diet being available in foodstuffs,conferring the potential of this compound to be supplemented by dietary *** quinone’s nutritional role in mammalian health is supported by the extensive deficits in reproduction,growth,and immunity resulting from the dietary absence of pyrroloquinoline quinone,and as such,pyrroloquinoline quinone has been considered as a“new vitamin.”Although the classification of pyrroloquinoline quinone as a vitamin needs to be properly established,the wide range of benefits for health provided has been reported in many *** this respect,pyrroloquinoline quinone seems to be particularly involved in regulating cell signaling pathways that promote metabolic and mitochondrial processes in many experimental contexts,thus dictating the rationale to consider pyrroloquinoline quinone as a vital compound for mammalian *** the regulation of different metabolic mechanisms,pyrroloquinoline quinone may improve clinical deficits where dysfunctional metabolism and mitochondrial activity contribute to induce cell damage and *** quinone has been demonstrated to have neuroprotective properties in different experimental models of neurodegeneration,although the link between pyrroloquinoline quinone-promoted metabolism and improved neuronal viability in some of such contexts is still to be fully ***,we review the general properties of pyrroloquinoline quinone and its capacity to modulate metabolic and mitochondrial mechanisms in physiological *** addition,we analyze the neuroprotective properties of pyrroloquinoline quinone in different neurodegenerative conditions and consider future perspectives for pyrroloquinoline quinone’s potential in health and disease.

关键词： metabolism mitochondria neurodegenerative disease neuroprotection pyrroloquinoline quinone retinal diseases

Human retinal secretome:A cross-link between mesenchymal and retinal cells

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World Journal of Stem Cells 2023年第7期15卷 665-686页

作者： Luigi Donato Concetta Scimone Simona Alibrandi Sergio Zaccaria Scalinci Domenico Mordà Carmela Rinaldi Rosalia D'Angelo Antonina Sidoti Department of Biomedical and Dental Sciences and Morphofunctional Imaging University of MessinaMessina 98125Italy Department of Biomolecular Strategies Genetics and Cutting-Edge TherapiesEuro-Mediterranean Institute of Science and TechnologyPalermo 90139Italy Department of Chemical BiologicalPharmaceutical and Environmental SciencesUniversity of MessinaMessina 98125Italy Department of Medical and Surgical Sciences University of BolognaBologna 40121Italy

In recent years,mesenchymal stem cells(MSC)have been considered the most effective source for regenerative medicine,especially due to released soluble paracrine bioactive components and extracellular *** factors,collectively called the secretome,play crucial roles in immunomodulation and in improving survival and regeneration capabilities of injured ***,there has been a growing interest in the secretome released by retinal cytotypes,especially retinal pigment epithelium and Müller glia *** latter trophic factors represent the key to preserving morphofunctional integrity of the retina,regulating biological pathways involved in survival,function and responding to ***,these factors can play a pivotal role in onset and progression of retinal diseases after damage of cell secretory *** this review,we delineated the importance of cross-talk between MSCs and retinal cells,focusing on common/induced secreted factors,during experimental therapy for retinal *** cross-link between the MSC and retinal cell secretomes suggests that the MSC secretome can modulate the retinal cell secretome and vice *** example,the MSC secretome can protect retinal cells from degeneration by reducing oxidative stress,autophagy and programmed cell ***,the retinal cell secretome can influence the MSC secretome by inducing changes in MSC gene expression and phenotype.

关键词： Secretome Mesenchymal stem cells retinal cells Extracellular vesicles retinal diseases

Recent Developments in the Treatment of Wet Age-related Macular Degeneration

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Current Medical Science 2020年第5期40卷 851-857页

作者： Zois Papadopoulos Department of Ophthalmology Massachusetts Eye and Ear InfirmaryHarvard Medical SchoolBostonMassachusettsUSA

Age-related macular degeneration(AMD)is the most common cause of irreversible blindness and visual impairment in individuals over the age of 50 years in western *** than 25 million people currently suffer from this illness in the world,with an additional 500000 every year,*** is a multifactorial ocular disease that affects the maculae due to a late-onset progressive neurodegeneration and dysfunction of photoreceptors and retinal pigment epithelium(RPE).There are many subtypes of AMD but basically two broad forms:the nonneovascular(dry,nonexudative)and neovascular(wet,exudative).Exudative AMD is the less common form(about 15%)but tends to progress more *** the moment,wet AMD is treated primarily on the basis of anti-vascular endothelial growth factor(VEGF)agents,which have led to massive improvement in the prognosis of the disease since they were first *** article focuses on the latest treatment approaches to neovascular *** extensive literature review was performed in order to illustrate the effectiveness of current and future anti-VEGF agents as well as the landmark clinical studies that have been carried out to establish these drugs as a gold standard in the therapy of wet AMD.

关键词： neovascular age-related macular degeneration exudative AMD medical retina retinal diseases neovascularization angiogenesis vascular endothelial growth factor

N-acetylserotonin alleviates retinal ischemia-reperfusion injury via HMGB1/RAGE/NF-κB pathway in rats

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 228-238页

作者： Yu-Ze Zhao Xue-Ning Zhang Yi Yin Pei-Lun Xiao Meng Gao Lu-Ming Zhang Shuan-Hu Zhou Shu-Na Yu Xiao-Li Wang Yan-Song Zhao Department of Ophthalmology Affiliated Hospital of Weifang Medical UniversityWeifang 261031Shandong ProvinceChina School of Medical Imaging Weifang Medical UniversityWeifang 261053Shandong ProvinceChina Department of Anatomy Weifang Medical UniversityWeifang 261053Shandong ProvinceChina Departments of Orthopedic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA

AIM:To observe the effects of N-acetylserotonin(NAS)administration on retinal ischemia-reperfusion(RIR)injury in rats and explore the underlying mechanisms involving the high mobility group box 1(HMGB1)/receptor for advanced glycation end-products(RAGE)/nuclear factor-kappa B(NF-κB)signaling ***:A rat model of RIR was developed by increasing the pressure of the anterior chamber of the *** male Sprague Dawley were randomly divided into five groups:sham group(n=8),RIR group(n=28),RIR+NAS group(n=28),RIR+FPS-ZM1 group(n=8)and RIR+NAS+FPS-ZM1 group(n=8).The therapeutic effects of NAS were examined by hematoxylin-eosin(H&E)staining,and retinal ganglion cells(RGCs)*** expression of interleukin 1 beta(IL-1β),HMGB1,RAGE,and nod-like receptor 3(NLRP3)proteins and the phosphorylation of nuclear factorkappa B(p-NF-κB)were analyzed by immunohistochemistry staining and Western blot *** expression of HMGB1 protein was also detected by enzyme-linked immunosorbent assay(ELISA).RESULTS:H&E staining results showed that NAS significantly reduced retinal edema and increased the number of RGCs in RIR *** NAS therapy,the HMGB1 and RAGE expression decreased significantly,and the activation of the NF-κB/NLRP3 pathway was antagonized along with the inhibition of p-NF-κB and NLRP3 protein ***,NAS exhibited an anti-inflammatory effect by reducing IL-1β*** inhibitory of RAGE binding to HMGB1 by RAGE inhibitor FPS-ZM1 led to a significant decrease of p-NF-κB and NLRP3 expression,so as to the IL-1βexpression and retinal edema,accompanied by an increase of RGCs in RIR ***:NAS may exhibit a neuroprotective effect against RIR via the HMGB1/RAGE/NF-κB signaling pathway,which may be a useful therapeutic target for retinal disease.

关键词： retinal diseases retinal ischemia—reperfusion injury N-acetylserotonin high mobility group box 1 receptor for advanced glycation end-products nuclear factor-κB rats

Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen

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International Journal of Ophthalmology(English edition) 2022年第6期15卷 868-875页

作者： Jia Luo Pei-Quan Zhao Hao-Jie Chen Miao-Miao Liu Jia-Qi He Ping Fei Department of Ophthalmology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Urology Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China department of General Surgery Huadong HospitalFudan UniversityShanghai 200040China

AIM:To investigate the role of procollagen C-proteinase enhancer 1(PCPE1)in retinal angiogenesis and relevant ***:The Pcolce1-knockout(KO)mice were used to explore the effect of PCPE1 on retinal angiogenesis in ***1 si RNA were designed,cell count kit 8(CCK8)assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells(h RMECs)in *** embryo fibroblasts(MEF)cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen ***:In vivo studies showed that the retinal vascular density of Pcolce1-/-mice was significantly lower than that of the control ***,silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of h RMECs in ***,much more procollagen was found in Pcolce1-/-MEF cells,compared to wild type MEF ***:PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen,which may provide a potential therapeutic target of retinal vascular disease.

关键词： retinal diseases angiogenesis procollagen C-proteinase enhancer 1 collagen

Association between central serous chorioretinopathy and Helicobacter pylori infection: a systematic review and Meta-analysis

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1120-1127页

作者： Da-Wen Wu Fei-Peng Jiang Ge Ge Mei-Xia Zhang Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Research Laboratory of Macular Disease West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina

AIM:To investigate the association between central serous chorioretinopathy(CSC)and Helicobacter pylori(Hp)by summarizing all available ***:The Scopus,Embase,EBSCO,PubMed,Web of Science,and Cochrane Library databases for all relevant studies published from inception to October 2022 were searched,and manually searched for relevant reference lists as a *** investigating the association between CSC and Hp infection were ***,8 case-control studies were included in the Meta-analysis after study ***:The results showed no significant correlation between Hp infection and CSC[odds ratio(OR)1.89,95%confidential interval(CI)0.58–6.15,I2=96%,P=0.29].After subgroup analysis based on the degree of development of the study(developing/developed countries),it was found that the results of the two subgroups were the same as the whole,and no significant difference between the two subgroups ***-regression showed that the effect of sample size on heterogeneity among studies was more prominent(P<0.01,adjusted R^(2)=89.72%),which can explain 89.72%of the sources of ***:This Meta-analysis reveals no significant correlation between Hp infection and CSC,which still warrants further well-designed extensive sample studies to reach a more reliable conclusion and promote a better understanding of the treatment of CSC.

关键词： central serous chorioretinopathy Helicobacter pylori infection retinal diseases Meta-analysis

retinal layer segmentation using gradient feature calculation in OCT

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Journal of Innovative Optical Health Sciences 2024年第6期17卷 137-147页

作者： Lei Liu Yeman Liu Xiaoteng Yan Haiyi Bian Hang Xu Chunzhong Li Hongnan Duan Faculty of Electronic Information Engineering Huaiyin Institute of Technology Huai'an Jiangsu 223003P.R.China Department of Ophthalmology Affiliated Huai'an Hospital of Xuzhou Medical University Huai'an Jiangsu 223003P.R.China

retinal diseases pose significant challenges to global healthcare systems,necessitating accurate and efficient diagnostic *** Coherence Tomography(OCT)has emerged as a valuable tool for diagnosing and monitoring retinal conditions due to its noncontact and noninvasive *** paper presents a novel retinal layering method based on OCT images,aimed at enhancing the accuracy of retinal lesion *** method utilizes gradient analysis to effectively identify and segment retinal *** selecting a column of pixels as a segmentation line and utilizing gradient information from adjacent pixels,the method initiates and proceeds with the layering *** approach addresses potential issues arising from partial layer overlapping,minimizing deviations in layer *** results demonstrate the efficacy of the proposed method in accurately segmenting eight retinal boundaries,with an average absolute position deviation of 1.75 *** providing accurate segmentation of retinal layers,this approach contributes to the early detection and management of ocular conditions,ultimately improving patient outcomes and reducing the global burden of vision-related ailments.

关键词： retinal diseases optical coherence tomography retinal layer segmentation.