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Sequence analysis for the complete proviral genome of reticuloendotheliosis virus Chinese strain HA9901

Science China(Life Sciences) 2006年第2期49卷 149-157页

作者： WANG Yu CUI Zhizhong JIANG Shijin College of Animal Science and Veterinary Medicine Shandong Agricultural University Taian 271018 China College of Animal Science and Veterinary Medicine Shandong Agricultural University Taian 271018 China College of Animal Science and Veterinary Medicine Shandong Agricultural University Taian 271018 China

The genomic DNA extracted from chicken embryo fibroblast (CEF) infected with a Chi-nese field isolate HA9901 of reticuloendotheliosis virus (REV) was used as the template to amplify the REV proviral genomic cDNA by PCR with 6 pairs of primers according to published sequences. Six overlapping fragments were amplified, cloned into the TA vector and sequenced, including a fragment which was amplified from the circular proviral cDNA and covering both 5′- and 3′-ends. The complete sequence of the whole genome was established and analyzed with a DNAstar software. Comparisons of the sequence with two other strains demonstrated that the genomes of REV were relatively con-servative, the homogenecity for all genes or LTR fragments of the 3 strains was over 92%, no matter whether they were isolated from different species and regions in different years. But, the homology of Chinese strain HA9901 to a fowl pox virus-associated strain from Chickens was higher than that to strain SNV isolated from ducks.

关键词： reticuloendotheliosis virus, the genomic sequence, homology comparison.

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Isolation of recombinant field strains of Marek's disease virus integrated with reticuloendotheliosis virus genome fragments

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Science China(Life Sciences) 2005年第1期48卷 81-88页

作者： ZHANG Zhi CUI Zhizhong Department of Preventive Veterinary Medicine College of Animal Science and Veterinary Medicine Shandong Agriculture UniversityTaian271018 China Department of Preventive Veterinary Medicine College of Animal Science and Veterinary Medicine Shandong Agriculture UniversityTaian271018 China

Two Marek's disease virus (MDV) field strains were isolated from chickens with tumors independently from Guangdong and Guangxi provinces, and it was confirmed that there were no co-infections with reticuloendotheliosis viruses (REV) in chicken embryo fibroblast cells (CEF) in indirect fluorescence antibody test (IFA) with REV-specific monoclonal antibodies. By dot blot hybridization and PCR of genomic DNA of MDV-infected CEF, it was indicated that LTR fragments of REV genome were integrated into genome of these two MDV field strains. To amplify and clone the integrated REV LTR with MDV sequence at the junction, 4 primers from REV LTR and 7 primers from MDV genome fragment with REV LTR insertion hot points were synthesized and 28 (4x7) pairs of primers (one from REV and another from MDV for each pair) were used in PCR while using the genomic DNA of both strains as the templates. The sequence data demonstrated that both recombinant field strains contained the same REV LTR inserted into MDV at the identical sites in US fragment of the genomes. From the above, it was speculated that both recombinant field MDVs were originated from a same recombinant virus and spread among chicken flocks in two provinces.

关键词： reticuloendotheliosis virus, Marek's disease viruses, recombinant field strain.

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REACTIVE RETICULOSIS

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中华医学杂志(英文版) 1964年第11期83卷 763-771页

作者：黄克维刘海川

*** cases of reactive reticu-losis were *** importantclinical manifestations were irregularfever,enlargement of the liver andspleen,and leukopenia,accompanied byfrequent skin rash and intestinal ulcera-tions,and the main pathologic findingsconsisted of marked hyperplasia of thereticuloendothelial cells in the liver,spleen,lymph nodes and bone marrowwith preservation of the normal archi-tecture of the spleen and lymph *** pathogenesis of reactivereticulosis was discussed,and allergy todrugs was suggested.

关键词： BONE MARROW CLASSIFICATION DIAGNOSIS, DIFFERENTIAL DRUG HYPERSENSITIVITY LIVER LYMPH NODES PATHOLOGY reticuloendotheliosis SPLEEN

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