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Distribution of Eight Blast-resistant genes in Parents of Three-line Japonica Hybrid Rice

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Plant Diseases and Pests 2018年第2期9卷 1-5,15页

作者： Chu Huangwei Cheng Can Niu Fu’an Zhou Jihua Tu Rongjian Luo Zhongyong Wang Xinqi Cao Liming Crop Breeding and Cultivation Research Institute Shanghai Academy of Agricultural Sciences

The distribution of rice blast-resistant genes Pi2, Pi5, Pi9, Pi54, Pia, Pib, Pit and Pita in 36 restoring lines and 42 sterile lines were detected using functional molecular markers. The results indicated that those eight blast-resistant genes distributed in the 78 tested lines at various frequencies. Six rice blast-resistant genes were detected in the sterile line Shen 11 A, containing the most rice blast-resistant genes among the 78 lines, whereas no rice blast-resistant gene was detected in the lines 8527 A, Xu1 A and 016 A. The genetic distance-based clustering analysis showed that those 78 lines could be divided into five groups.

关键词： Hybrid japonica rice Rice blast resistance gene

Mapping QTL for high-temperature adult-plant resistance and all-stage resistance to stripe rust in winter wheat Druchamp

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Mapping QTL for high-temperature adult-plant resistance and ...

中国植物病理学会2015年学术年会

作者： HOU Lu CHEN Xian-ming WANG Mei-nan JING Jin-xue Key Laboratory of Agricultural Integrated Pest Management Institute of Plant ProtectionQinghai Academy of Agriculture and Forestry Sciences Department of Plant Pathology Washington State University State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection Northwest A&F University

Winter wheat Druchamp has both high-temperature adult-plant(HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformis ***(Pst).The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940 *** map the quantitative trait loci(QTL) for stripe rust resistance,an F recombinant inbred line(RIL) population from cross Druchamp x MichiganAmber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions;and genotyped with simple sequence repeat(SSR) and single nucleotide polymorphism(SNP) *** interval mapping(CIM)identified eight HTAP resistance QTL and three all-stage resistance *** the eight HTAP resistance QTL,***-1BL.2(explaining 2.36%31.04%variation),***-2BL(2.81%5.65%),***-SAL(2.27%7.22%) and ***-5BL.2(2.42%5.13%) were significant in all tests;and ***-lBL 1(1.94%10.19%),***-1DS(2.04%7.24%),***-3AL(1.78%3.85%) and ***-6BL.2(1.69%3.71%) were significant in some of the *** three all-stage resistance QTL,***-5BL.1(5.47%6.04%),***(9.27%1.94%) and ***-6BL.1(13.07%0.36%),were detected based on reactions in the seedlings tested with certain Pst *** the eleven QTL detected in Druchamp,at least three(*** for race-specific all-stage resistance and ***-3AL and ***-6BL.2 for race non-specific HTAP resistance) are *** these QTL,especially those for durable HTAP resistance,and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust.

关键词： stripe rust resistance gene SNP,QTL

Development of Chinese near-isogenic lines of rice and their differentiating ability of pathogenic races of blast fun-gus

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Chinese Rice Research Newsletter 2002年第1期10卷 9-10页

作者： LINGZhongzhuan T．W．Mew 等 Inst of Crop Breeding and Cultivation CAAS Beijing 100081 China PBGN Division IRRI PO Box 933 Manila Philippines

To use common differential varieties in the world, a set of international differential varieties, including 6 indica and 2 japonica rice varieties, were set up in the middle of 1960s; in 1976, a set of differential va... 详细信息

关键词： pathogenic races resistance gene japonica rice the world

Identification of genes for Powdery Mildew resistance in Mungbean

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Journal of Life Sciences 2010年第5期4卷 25-29页

作者： Parinya Khajudpam Sopone Wongkaew Piyada Tantasawat School of Crop Production Technology Suranaree University of Technology Nakhon Ratchasima 30000 Thailand Center for Agricultural Biotechnology （AG-BIO / PERDO-CHE） Bangkok 10330 Thailand

Powdery mildew, which called Sphaerotheca phaseoli in Latin, is one of the major diseases of mungbean （Vigna radiata L.） worldwide, causing up to 50% yield losses. Most mungbean varieties grown in Thailand are susceptible to the disease, therefore, new resistant varieties are highly desirable. Three resistant mungbean lines, V4718, V4758 and V4785, were identified from the AVRDC collection. In this study, the authors compared the resistance levels among these 3 lines and tested the allelic relationship among these resistance genes. Three crosses, V4718 × V4758, V4718 × V4785 and V4758 × V4785, were made and the F1 hybrids were selfed to generate the F2 populations and crossed to a susceptible variety, CN72 to generate the F1 × S populations. In the F1 × S and F2 populations, the resistance segregated in a ratio of 3 Resistant （R）：I Susceptible （S） and 15R：IS, respectively for all three crosses. These results indicate that a single dominant gene confers resistance to powdery mildew in each resistant line and these resistance genes are non-allelic. The authors are currently transferring these resistance genes into commercial varieties to provide durable resistance to powdery mildew.

关键词： Allelism resistance gene Sphaerotheca phaseoli Vigna radiata (L.)

Study on the relationship between structural diversity and drug resistance function of macrolide resistance-related genes in Pseudomonas aeruginosa

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Study on the relationship between structural diversity and d...

2019年浙江省医学会耳鼻咽喉头颈外科学学术大会

作者： Qing Chen Liyan Ni The Second Affiliated Hospital and Yuying Children’s Hospital Wenzhou Medical University

Objective In this work,to analyze the resistance mechanism of Pseudomonas aeruginosa to macrolide *** and methods:Pooled genomic DNAs of 262 clinical *** strains were sequenced,and PCR was applied to screen for macrolide resistance gene-positive *** cloning was performed to clone the resistance genes,and minimum inhibitory concentrations(MICs) were tested by the agar dilution ***:only two macrolide resistance genes,mphE and msrE,were identified in the pooled genomic DNA of the 262 *** *** screening results demonstrated that 4 strains(1.53%,4/262) carried both msrE and mphE,and an additional 3 strains(1.15%,3/262) carried only *** assay results showed that both the cloned msrE and mphE genes(pUCP18-mphE/DH5α and pUCP18-msrE/DH5α) were functional and mediated higher resistance levels to three second-generation macrolides(MIC levels increased≥ 4-fold to roxithromycin,clarithromycin and azithromycin compared with those of the recipient,DH5α) than to two first-generation macrolides(MIC levels increased≤2-fold to erythromycin and kitasamycin compared with those of the recipient,DH5α).Both the mphE and msrE genes conferred the highest resistance to roxithromycin(MICs:1024 μg/mL),but they showed the least resistance to azithromycin(MICs:8 μg/mL and 32 μg/mL,respectively).Analysis of the polymorphism of the proteins encoded by the msrE and mphE genes revealed that among the 6 mphE and 9 msrE variants(including two mphE and two msrE sequences from this work,while the remainder were obtained from a database),the two genes are very *** genes had one to three amino acid differences compared with the reference sequences for both genes(MG585957.1 for msrE and CP029638.1 for mphE).Conclusion:It can be concluded that the known macrolide resistance genes are seldom identified in clinical *** strains isolated in Wenzhou,China,despite several mphE and msrE genes being present in some ***

关键词： Pseudomonas aeruginosa macrolide resistance gene mphE msrE

Effects of promoters, the Woodchuck hepatitis post-transcriptional regulatory element, and weakened selection markers on transgene expression in transfected Chinese hamster ovary cells

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Effects of promoters, the Woodchuck hepatitis post-transcrip...

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Tian-Yun Wang Wen Wang Si-Jia Chen Qin Li Fang Wang Li Wang Xian-Jun Yang Department of Biochemistry and Molecular Biology Xinxiang Medical University International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan Xinxiang Medical University College of Pharmacy Xinxiang Medical University

Chinese hamster ovary(CHO) cells are the host of choice for the production of recombinant proteins in mammalian expression ***,we investigated the effects of promoters,the Woodchuck hepatitis posttranscriptional regulatory element(WPRE),and selection markers on transgene expression in CHO *** cytomegalovirus major(CMV) and Simian virus 40(SV40) promoters,were combined with the G418 weakened resistance gene and WPRE cisacting element to generate the expression *** results showed that the G418 weakened resistance gene increased transgene expression driven by the CMV and SV40 ***,WPRE combined with the G418 resistance gene obviously upregulated gene expression in CHO ***,WPRE showed no enhancing effects when the G418 weakened resistance gene was used,suggesting that the effects of WPRE were selection marker *** addition,WPRE combined with the G418 resistance gene showed higher translational efficiency and transient transgene expression compared with the control ***,analysis of cetuximab expression also showed that the CMV promoter combined with WPRE expression produced higher protein levels than the CMV promoter *** conclusion,the CMV promoter combined with the WPRE had positive effects on the regulation of CHO expression and could be used for recombinant protein production.

关键词： Chinese hamster ovary WPRE Promoter Weakened resistance gene Transgene

Virulence comparison of Magnaporthe oryzae isolates between USA and Jilin of China and utilizing strategies of resistance to blast genes

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Virulence comparison of Magnaporthe oryzae isolates between ...

中国植物病理学会2015年学术年会

作者： WANG Ji-chun JIA Yu-lin LU Bao-hui WU Xian REN Jin-ping Institute of Plant Protection Jilin Academy of Agricultural Sciences U.S.Department of Agriculture-Agricultural Research Service Dale Bumpers National Rice Research Center

Rice blast is one of the most devastating crop diseases *** and planting blast-resistance cultivars are economic,effective and environment-friendly method to control rice *** master die isolates virulence and identify broad spectrum resistance gene types based on rice cultivars pathotypes interacted with representative strains are very important pathways for directing *** this report,44 isolates from Jilin Province and 14 race from USA of *** were inoculated onto 24 international rice monogenic lines（MLs） carrying a major blast resistance gene-Pia,Pib,Pii,Pik,Pik-h,Pik-m,Pik-p,Pik-s,Pish,Pit,Pita,Pita-2,Piz,Piz-t,Pil,Piz-5,Pi3,Pi5（t）,Pi7（t）,Pi9,Pi11（t）,Pi12（t）,PH9 and Pi20- and the susceptible reeurrent parent,Lijiangxintuanheigu（LTH）,under greenhouse *** percentage of pathotypes of the 44 isolates was found ranging from 8.3% to 79.2%,the frequency of resistant reactions of the MLs carrying Pi9,Pi19,Piz and Piz-5 were 94.2%,84.1%,81.8% and 81.8% in JiLin Province,*** 14 Magnaporthe oryzae isolates of USA were pathogenic to eight or more MLs,and the frequency of pathogenicity was ranged from 33.3%（race IG1） to 87.5%（race IE1）.The ML carrying Pi9 and Pita-2 demonstrated broad spectrum resistance to 14 representative USA races or isolates,and their resistance frequency was 92.9% and 78.6%,*** result provided import information for introducing and exchanging germplasms from ***,the pyramiding strategies with blast R genes for improving blast resistance in breeding lines were in progress and will be presented.

关键词： Magnaporthe oryzae rice blast monogenic line resistance gene breeding

Variable AvrXa23-like TALEs enable Xanthomonas oryzae pv. oryzae to overcome Xa23 resistance in rice

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Variable AvrXa23-like TALEs enable Xanthomonas oryzae pv. or...

中国植物病理学会2021年学术年会

作者： XU Zhengyin YANG Yangyang LIU Linlin WANG Yijie XU Xiameng ZOU Lifang CHEN Gongyou School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong University

The dominant resistance gene,Xa23,mediates broad-spectrum resistance in rice to bacterial leaf blight(BLB) caused by Xanthomonas oryzae ***(Xoo) strains containing an avirulence gene avrXa23 which encodes a transcription activator-like effector(TALE) protein that binds to the EBE(effector binding element) of Xa23 *** is unclear whether the considerable pressure of Xa23 leads to the emerging of Xoo isolates that overcome Xa23 resistance in ***,we found that a virulent isolate,AH28 from Anhui province,is compatible with the Xa23-containing rice CBB23,of185 Xoo isolates collected from rice-growing areas in *** the genome,assembling the TALE repertoire,and isolating tal genes of AH28 revealed an ortholog of avrXa23,tal7 b of ***4 RVDs(repeat-variable diresidues) in Tal7 b are missed and the 5 and 8 RVDs changed from NG and NS to NS and S,which made the Tal7 b protein unable to induce the expression of Xa23 in *** ectopic expression of AH28 tal7 b in a tal-free mutant pH of PX099 and avrXa23 in AH28 made the pH strain still be compatible with and the AH28 strain incompatible with Xa23 rice,*** the similar RVDs of AvrXa23 from the available genome sequences,we found there are eight variable AvrXa23-like TALEs in Xoo,suggesting that the RVD mutation in a TALE protein may be a common strategy for the pathogen evolution to avoid being "trapped" by an executor R *** to our knowledge,this is the first insight of a naturally-emerging Xoo isolate that overcomes the broadspectrum resistance of Xa23 by the variable AvrXa23-like TALEs.

关键词： resistance gene Xa23 Xanthomonas oryzae

Transcription profile analysis of interaction mechanism between sugarcane and Sporisorium scitamineum using microarray

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Transcription profile analysis of interaction mechanism betw...

2019年中国作物学会学术年会

作者：黄宁 Hui Ling Weihua Su Yachun Su Youxiong Que Key Laboratory of Sugarcane Biology and genetic Breeding (Fujian) Ministry of Agriculture Fujian Agriculture and Forestry University Key Laboratory of Crop genetics and Breeding and Comprehensive Utilization Ministry of Education Fujian Agriculture and Forestry University

【Research background】 Sugarcane production is seriously affected by Sporisorium scitamineum infection in the world. Although some excellent resistant breeding materials have been created, the utilization of sugarcane germplasm and the breeding of resistant varieties are still greatly restricted for the complex genetic background of sugarcane and the differentiation of smut race. To solve this problem, much effort should be devoted to underlying the mechanism of sugarcane smut resistance and S. scitamineum virulence. 【Materials and methods】 According to our previous RNA-seq and smut pathogen genome data, the gene expression chip was customized. The transcription profile of gene set, which referred to 2,0392 from sugarcane and the whole CDS sequence(6,621) from S. scitamineum genome, was identified on smut spore injected samples. These samples were from sugarcane smut-susceptible genotype YC71-374 and smut-resistant genotype NCo376. 【Result and analysis】 During the interaction between sugarcane and smut fungus, 6,842 sugarcane genes and 3,569 S. scitamineum genes were differentially expressed. According to the GO and KEGG enrichment analysis, the differential expressed sugarcane genes involved in primary metabolism pathway, MAPK pathway, plant hormone signal transduction pathway and ROS response, the differential expressed S. scitamineum genes mostly located in vesicles, and have activities like transportation, signal transduction and redox. After that, the differential expressed genes from sugarcane and S. scitamineum were selected and used to construct the weighted gene co-expression(WGCNA) network of sugarcane-S. scitamineum interaction. Finally, two sugarcane smut-resistance related genes(vacuolar-sorting receptor, ScVSR;and leucine-rich repeat receptor-like protein kinase, Sc LRR-RLK) and four putative S. scitamineum effector genes, including protein essential during penetration 1(SsPep1), hypothetical cysteine-protease inhibitor(SsE4), conserved hypothetical pro

关键词： sugarcane Sporisorium scitamineum microarray WGCNA resistance gene effector protein