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Characterization and Molecular Mapping of a Stripe Rust resistance gene in Synthetic Wheat CI110

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Journal of Integrative Agriculture 2012年第4期11卷 521-527页

作者： REN Qiang LIU Hui-juan ZHANG Zeng-yan FENG Jing XU Shi-chang PU Zong-jun XIN Zhi-yong The National Key Facility for Crop gene Resources and genetic Improvement/Key Laboratory of Biology and genetic Improvement of Triticeae Crops Ministry of Agriculture/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China Crop Research Institute Sichun Academy of Agricultural Sciences Chengdu 610066 P.R. China

Stripe rust,caused by Puccinia striiformis ***（Pst）,is one of the most destructive diseases of wheat（Triticum aestivum L.）.To diversify stripe rust-resistant resources for wheat breeding programs,a CIMMYT synthetic wheat line CI110 was identified to be resistant to 28 isolates of Pst,including 6 Chinese prevalent races *** analysis indicated that a single dominant gene was responsible for the stripe rust resistance in CI110,temporarily designated YrC110.A molecular map,harboring YrC110 and 9 linked SSR markers,was constructed through simple sequence repeat（SSR）,and bulked segregant *** linked markers and YrC110 were assigned on the short arm of chromosome 1B using the Chinese Spring nullisomic-tetrasomic and ditelosomic *** postulation based on seedling reaction patterns to 30 Pst isolates suggested that the resistance gene YrC110 seemed different from the other known resistance genes tested,such as Yr9,Yr10,Yr15,Yr24,and Yr26/*** SSR markers Xbarc187150,Xgwm18227,Xgwm11223,and Xbarc240292 distinguished YrC110 from Yr10,Yr15,Yr24,and Yr26/YrCH42,and could be used as diagnostic ones for YrC110 in wheat resistant breeding programs against stripe rust.

关键词： synthetic wheat Puccinia striiformis f.sp.tritici resistance gene simple sequence repeat(SSR) marker gene postulation

High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1

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The Crop Journal 2023年第6期11卷 1852-1861页

作者： Jian Wang Hongyu Li Tao Shen Shikai Lyu Shams ur Rehman Hongna Li Guiping Wang Binyang Xu Qing Wang Wanyi Hu Kairong Li Shengsheng Bai Jian Ma Haitao Yu Matthew N.Rouse Shisheng Chen School of Advanced Agriculture Sciences Peking UniversityBeijing 100871China National Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural SciencesShandong Laboratory of Advanced Agricultural Sciences in WeifangWeifang 261325ShandongChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Wheat Research Institute Weifang Academy of Agricultural SciencesWeifang 261071ShandongChina US Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory and Department of Plant PathologyUniversity of MinnesotaSt.PaulMN 55108USA

Stem rust,caused by Puccinia graminis ***(Pgt),threatens global wheat *** of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the *** this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line *** seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races *** a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference *** regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate *** them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line *** markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes *** presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)*** genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.

关键词： Durum wheat Stem rust resistance gene Sr8155B1 CC-NBS-LRR

Fine Mapping of RppP25, a Southern Rust resistance gene in Maize

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Journal of Integrative Plant Biology 2013年第5期55卷 462-472页

作者： Panfeng Zhao Guobin Zhang Xiaojun Wu Na Li Dianyi Shi Dengfeng Zhang Chunfang Ji Mingliang Xu Shoucai Wang National Maize Improvement Center of China Key Laboratory of Biology and Genetic Improvement of Maize (Ministry of Agriculture) China Agricultural University National Key Facility for Crop gene Resources and genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences

Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 （as the recurrent parent）. The 1：1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat （SSR） markers and seven insertion/deletion （InDel） markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat （NBS-LRR） protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.

关键词： Fine mapping maize Puccinia polysora resistance gene southern rust.

Molecular Mapping of a Stripe Rust resistance gene YrH9020a Transferred from Psathyrostachys huashanica Keng on Wheat Chromosome 6D

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Journal of Integrative Agriculture 2014年第12期13卷 2577-2583页

作者： LIU Ze-guang YAO Wei-yuan SHEN Xue-xue CHAO Kai-xiang FAN Yu LI Min-zhou WANG Bao-tong LI Qiang JING Jin-xue State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

Stripe rust （yellow rust）, caused by Puccinia striiformis f. sp. tritici （Pst）, is one of the most devastating diseases of wheat throughout the world. H9020-1-6-8-3 is a translocation line originally developed from interspeciifc hybridization between wheat line 7182 and Psathyrostachys huashanica Keng and is resistant to most Pst races in China. To identify the resistance gene（s） in the translocation line, H9020-1-6-8-3 was crossed with susceptible cultivar Mingxian 169, and seedlings of the parents, F1, F2, F3, and BC1 generations were tested with prevalent Chinese Pst race CYR32 under controlled greenhouse conditions. The results indicated that there is a single dominant gene, temporarily designated as YrH9020a, conferring resistance to CYR32. The resistance gene was mapped by the F2 population from Mingxian 169/H9020-1-6-8-3. It was linked to six microsatellite markers, including Xbarc196, Xbarc202, Xbarc96, Xgpw4372, Xbarc21, and Xgdm141, lfanked by Xbarc96 and Xbarc202 with at 4.5 and 8.3 cM, respectively. Based on the chromosomal locations of these markers and the test of Chinese Spring （CS） nullitetrasomic and ditelosomic lines, the gene was assigned to chromosome 6D. According to the origin and the chromosomal location, YrH9020a might be a new resistance gene to stripe rust. The lfanking markers linked to YrH9020a could be useful for marker-assisted selection in breeding programs.

关键词： Puccinia striiformis f. sp. tritici Psathyrostachys huashanica Keng resistance gene genetic analysis SSR markers

Characterization and fine mapping of the rice blast resistance gene Pia

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Science China(Life Sciences) 2011年第4期54卷 372-378页

作者： ZENG XiaoShan YANG XianFeng ZHAO ZhengHong LIN Fei WANG Ling PAN QingHua Laboratory of Plant resistance and genetics College of Natural Resources and Environment Sciences South China Agricultural University Guangzhou 510642 China Hunan Agricultural University Changsha 410128 China

Blast, caused by Magnaporthe oryzae, is one of the most widespread and destructive diseases of rice. Breeding durable resistant cultivars （cvs） can be achieved by pyramiding of various resistance （R） genes. Pia, carded by cv. Aichi Asahi, was evaluated against 612 isolates of M. oryzae collected from 10 Chinese provinces. The Pia gene expresses weak resistance in all the provinces except for Jiangsu. Genomic position-ready marker-based linkage analysis was carded out in a mapping population consisting of 800 F2 plants derived from a cross of Aichi Asahi×Kasalath. The locus was defined in an interval of approximately 90 kb, flanked by markers A16 and A21. Four candidate genes （Pia-1, Pia-2, Pia-3, and Pia-4）, all having the R gene conserved structure, were predicted in the interval using the cv. Nipponbare genomic sequence. Four candidate resistance gene （CRG） markers （A17, A25, A26, and A27）, derived from the four candidates, were subjected to genotyping with the recombinants detected at the flanking markers. The first three markers completely co-segregated with the Pia locus, and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers, indicating that the fourth candidate gene, Pia-4, could be excluded. Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 （Pia-3） perfectly matched the R gene genotype of Pia, indicating that Pia-3 is the strongest candidate gene for Pia.

关键词： rice blast resistance gene Pia t'me mapping genotype

Identification of Molecular Markers Linked to the Wilt resistance gene FuJ7(t) in Flax

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Agricultural Sciences in China 2004年第7期3卷 502-508页

作者： BOTian-yue WUAi-zhong YEHua-zhi LIXiao-bing ZHULi-huang HorticulturalResearchInstitute ShanghaiAcademyofAgriculturalSciencesShanghai201106P.R.China CollegeofAgriculture SichuanAgriculturalUniversityYa＇an625014P.R.China InstituteofgeneticsandDevelopmentalBiology ChineseAcademyofSciencesBeijing100101P.R.China

A cross between wilt resistant flax variety Jinya7 and susceptible variety Jinya1 wasmade for mapping wilt resistance gene(s). The inoculation test of F1 and F2 progeny provedthat the resistance of Jinya7 to wilt is controlled by two dominant genes. With 48 EcoRⅠ/MseⅠ primer combinations, amplified fragment length polymorphisms (AFLP) analysis wasperformed on two parents and their F2 resistance and susceptibility bulks. A total ofabout 3300 distinguishable bands were amplified, of which three bands had stabledifferences. The genetic linkage analysis of the three polymorphic DNA fragments withthe resistance gene(s) was made in the F2 segregating population derived from the crossbetween Jinya7 and Jinya1. The DNA fragment AG/CAG was found closely linked to one of thewilt-resistant genes, which with a genetic distance of 5.2cm, was tentatively named FuJ7(t).The cloned fragment AG/CAG was sequenced and then converted successfully to a sequencecharacterized amplified region (SCAR) marker, which can be used more conveniently in theidentification and marker-assisted selection for the wilt resistance gene FuJ7(t) toflax wilt.

关键词： Flax Fusarium wilt resistance gene Molecular marker AFLP

Identification of Co-Segregating RAPD Marker Linked to Powdery Mildew resistance gene Pm18 in Wheat

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Agricultural Sciences in China 2004年第6期3卷 409-415页

作者： ZHANGQing-li GUFeng LITao GAOJu-rong WANGHong-gang AgronomyCollege ShandongAgriculturalUniversityTai＇an271018P.R.China SubcenterofNationalWheatImprovementCenter Tai＇an271018P.R.China

The Pm18 gene of wheat confers resistance to the powdery mildew which is one of the mostserious diseases in many regions of the world. In this study, bulked segregant analysis(BSA) was used to develop randomly amplified polymorphic DNA (RAPD) markers linked toPm18 gene. Three hundred and twenty decamer primers were screened and one of them wasidentified as RAPD marker (S411600) linked to Pm18. Using the F2 mapping population fromthe cross Pm18Chancellor, the marker S411600 was shown to co-segregate with the genePm18. This marker can be conveniently used for marker-assisted selection in wheatbreeding programs for the identification or pyramiding of Pm18 with other resistancegenes.

关键词： Wheat RAPD BSA Powdery mildew resistance gene

Virulence of Xanthomonas oryzae pv. oryzae on Rice Near-Isogenic Lines with Single resistance gene and Pyramiding Lines in China

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Agricultural Sciences in China 2004年第10期3卷 764-769页

作者： LIUHong-xia LIUFeng-quan HUBai-shi YANGWan-feng CHENZhi-yi XUZhi-gan DepartmentofPlantPathology NanjingAgriculturalUniversityNanjing210095P.R.China InstituteofPlantProtection JiangsuAcademyofAgriculturalSciencesNanjng210014P.R.China

Ninety one isolates of Xanthomonas oryzae pv. oryzae were collected from different rice- growing regions in China and determined for their virulence on 24 rice near-isogenic lines containing single resistance gene and 2-4 genes: IRBB1 (Xa1), IRBB2 (Xa2), IRBB3 (Xa3), IRBB4 (Xa4), IRBB5 (xa5), IRBB7 (Xa7), IRBB8 (xa8), IRBB10 (Xa10), IRBB11 (Xa11), IRBB13 (xa13), IRBB14 (Xa14), IRBB21 (Xa21), IR24 (Xa18), IRBB50 (Xa4 + xa5), IRBB51 (Xa4 + xa13), IRBB52 (Xa4 + Xa21), IRBB53 (xa5 + xa13), IRBB54 (xa5 + Xa21), IRBB55 (xa13 + Xa21), IRBB56 (Xa4 + xa5 + xa13), IRBB57 (Xa4 + xa5 + Xa21), IRBB58 (Xa4 + xa13 + Xa21), IRBB59 (xa5 + xa13 + Xa21) and IRBB60 (Xa4 + xa5 + xa13 + Xa21). The results showed that most isolates were less virulent on lines with more than one genes pyramided than those with single resistance gene. The isolates tested were more virulent on IR24 and IRBB10, less virulent on IRBB5, IRBB7 and IRBB21. Based on interactions between isolates and rice near-isogenic lines, 7 cultivars with single gene (IRBB5, IRBB4, IRBB3, IRBB14, IRBB2, IRBB1 and IR24) were chosen as the differentials, and the tested isolates were classified into 7 virulence groups. The reaction patterns of the 7 groups in order were: RRRRRRR, RRRRRRS, RRRRRSS, RR/SRRSSS, RRRSSSS, RRSSSSS, RSSSSSS. The virulence frequencies were 7.69, 6.59, 14.29, 12.09, 14.29, 28.57 and 16.48% respectively. The elementary system for races identification has been established in China based on the results. It will be possible to compare with races in other countries, and the results will facilitate the development of rice resistance breeding to bacterial blight in China.

关键词： Rice lines Xanthomonas oryzae pv. oryzae Virulence resistance gene

Molecular Tagging of a New resistance gene to Maize Dwarf Mosaic Virus Using Microsatellite Markers

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Acta Botanica Sinica 2002年第2期44卷 177-180页

作者：吴建宇汤继华夏宗良陈伟程河南农业大学农学院郑州450002

With joint analysis based on the parents, F 1, F 2 and backcrosses, the authors found that the resistance of the maize inbred line Huangzaosi to the maize dwarf mosaic virus strain B was conditioned by a major gene and polygene, and identified a new major gene. Bulked segregate and microsatellite analysis of a F 2 progeny from the combination of Huangzaosi×Mo17 were used to identify the resistance gene, mdm1(t), on the long arm of chromosome 6. This new resistance gene is tightly linked to and located between the microsatellite markers loci, phi077 and bnlg391. The linkage distances between phi077-mdm1(t) and mdm1(t)-bnlg391 are 4.74 centiMorgan (cM) and 6.72 cM respectively.

关键词： maize maize dwarf mosaic virus resistance gene microsatellite molecular tagging