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Heterozygosity of Knob-Associated Tandem Repeats and Knob Instability in Mitotic Chromosomes of Zea （Zea mays L. and Z. diploperennis Iltis Doebley）

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Journal of Integrative Plant Biology 2005年第11期47卷 1345-1351页

作者： Zhi-Yong XIONG Yong LIU Yong-Gang HE Yun-Chun SONG Ke-Xiu LI Guan-Yuan HE China-UK HUST-RRes （Rothamsted Reseach UK） Genetic Engineering and Genomics Joint Laboratory Huazhong University of Science and Technology （HUST） Wuhan 430074 China Key Laboratory of the Ministry of Education for Plant Developmental Biology Wuhan University Wuhan 430072 China

Knobs are blocks of heterochromatin present on chromosomes of maize （Zea mays L.） and its relatives that have effects on the frequency of genetic recombination, as well as on chromosome behavior. Knob heterozygosity and instability in six maize inbred lines and one Z. diploperennis Iltis Doebley line were investigated using the fluorescence in situ hybridization （FISH） technique with knob-associated tandem repeats （180 bp and 350 bp （TR- 1）） as probes. Signals of seven heterozygous knobs containing 180- bp repeats and of one heterozygous knob containing TR- 1 were captured in chromosomes of all materials tested according to the results of FISH, which demonstrates that the 180-bp repeat is the main contributor to knob heterozygosity compared with the TR- 1 element. In addition, one target cell with two TR- 1 signals on one homolog of chromosome 2L, which was different from the normal cells in the maize inbred line GB57, was observed, suggesting knob duplication and an instability phenomenon in the maize genome.

关键词： fluorescence in situ hybridization knob repetitive dna sequence Zea diploperennis Z. mays.

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Knob-associated tandem repeats on mitotic chromosomes in Zea diploperennis, maize and theirs hybrids

Knob-associated tandem repeats on mitotic chromosomes in Zea...

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湖北省遗传学会第七次代表大会暨学术讨论会

作者： XIONG Zhiyong~(1.2), GAO Yuan~1, HE Guanyuan~2, GU Mingguang~3, GUO Lequn~3 & SONG Yunchun~(1*) (1. Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, Wuhan 430072, China 2. China-UK HUST-RRes Crop Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China 3. Institute of Genetics, The Chinese Academy of Sciences, Beijing 1000101, China)

Knob-associated tandem repeats, 180-bp repeats and TR-1 elements, together with 45S rdna were located on mitotic chromosomes of Zea diploperennis (DP), maize inbred line F102 and theirs hybrid. In DP, knobs on chromosomes 1S, 4S, 4L and 5S are composed predominantly of the 180-bp repeats. In addition, 180-bp repeats co-exited with TR-1 elements were also detected in other seven sites on six chromosome pairs. In maize inbred line F102, 180-bp repeats were present in chromosomes 7S and one homologue of chromosomes 8L. TR-1 elements appeared on satellite of chromosome 6 and no detectable hybridization site co-located with 180-bp repeats was observed in maize F102. Polymorphism of size, number, and distribution of 180-bp repeats and TR-1 were revealed among different chromosomes and between homologous chromosomes in these two species. Using these excellent landmarks, the interspecific hybrid of maize and DP were identified. The results presented suggestthat comparative analysis of 180-bp repeats and TR-1 elements may aid in understanding the genome organization and the evolution in Zea.

关键词： Zea mays Zea diploperennis repetitive dna sequence fluorescence in situ hybridization heterochromatic knob.

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