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Evaluation of genetic diversity and drought tolerance among thirtythree dichondra (Dichondra repens) genotypes

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Grass Research 2022年第1期2卷 54-61页

作者： Meng Tan Yao Ling Yan Peng Zhou Li Department of Turf Science and Engineering College of Grassland Science and TechnologySichuan Agricultural UniversityChengdu 611130China

Dichondra(Dichondra repens)is an important ground cover plant and is also used as a herbal medicine in *** of this study were to evaluate phenotypic and genetic diversities among 33 genotypes by using 18 simple sequence repeat(SSR)markers and to further identify the drought tolerance of these germplasms based on five physiological *** showed that natural variations in phenotypes including plant height,leaf area,leaf thickness,and petiole length were observed among 33 genotypes under well-watered *** 18 SSR primer pairs were found to be polymorphic and significant genetic variation was found in these *** addition,there were obvious differences in leaf relative water content(RWC),electrolyte leakage(EL),chlorophyll(Chl)content,photochemical efficiency(Fv/Fm),and performance index on absorption basis(PIABS)among 33 genotypes in response to a prolonged period of drought stress(46 d).Drought tolerance of 33 genotypes was then ranked by using subordinate function value analysis(SFVA)and the most drought-tolerant or-sensitive genotypes were identified as Dr5 or Dr29,*** component analysis(PCA)further classified 33 genotypes into group I(drought-tolerant),group II(droughtsensitive),and group III(medium types).Current findings showed that 18 selected SSR primers could be potentially used to analyze genetic diversity and varietal identification in dichondra ***-tolerant wild dichondra resources provide a rich genetic base for breeding of new cultivars.

关键词： repens breeding drought

马蹄金(Dichondra repens Forst.)组织培养和快速繁殖

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分子植物育种 2020年第10期18卷 3331-3340页

作者：王猛李晓旭张筝王玲霞杨涓梁文裕宁夏大学生命科学学院银川750021

为了探究马蹄金组织培养及快繁技术,本研究以马蹄金为材料,使用75%的酒精和10%NaClO对不同外植体(胚根,下胚轴,子叶)进行消毒处理,在不同激素浓度配比的MS培养基中进行丛生芽诱导、愈伤组织诱导及分化,成功建立了马蹄金的组培快繁体系... 详细信息

为了探究马蹄金组织培养及快繁技术,本研究以马蹄金为材料,使用75%的酒精和10%NaClO对不同外植体(胚根,下胚轴,子叶)进行消毒处理,在不同激素浓度配比的MS培养基中进行丛生芽诱导、愈伤组织诱导及分化,成功建立了马蹄金的组培快繁体系。结果表明:利用马蹄金下胚轴为外植体直接诱导不定芽效果较好,其最适程序为:流水冲洗30 min+75%酒精30 s+10%NaClO 9 min,污染率为5%,成活率为75%;下胚轴不定芽诱导的最适培养基为MS+1 mg/L 6-BA+0.3 mg/L NAA,诱导率为93.55%,出芽指数为8.86;胚根愈伤组织诱导的最适培养基为MS+1 mg/L 6-BA+0.5 mg/L NAA,诱导率为100%,下胚轴愈伤组织诱导的最适培养基为MS+0.5 mg/L 6-BA+1 mg/L NAA,诱导率为95.24%,子叶愈伤组织诱导的最适培养基为MS+0.3 mg/L 6-BA+0.5 mg/L NAA,诱导率为100%;愈伤组织不定芽分化的最适培养基为1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA,芽诱导率为20%,平均芽数为12.83;组培苗诱导生根的最适培养基为不加激素的1/2MS培养基;生根苗以瓶盖全开的炼苗方式移栽到以河沙为基质的育苗盒中成活率为100%,且添加蒸馏水与自来水对植株生长并无明显影响。本研究成功建立了马蹄金组织培养与快繁体系,为马蹄金种质资源改良及优质种苗生产提供了技术参考。

关键词：马蹄金(Dichondra repens Forst.) 组织培养快繁技术诱导

Main Components and Antioxidant Activity of Callisia repens Extracts

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Asian Agricultural Research 2019年第7期11卷 76-79页

作者： Bo LENG Shaoru CAI Xiumin LI Jiafu HUANG Yu XUE Zhichao LIN Wensong CHEN Chunxian LI Yutian PAN Engineering Technological Center of Mushroom Industry Minnan Normal University Zhangzhou 363000 China School of Management Xiamen University Tan Kah Kee College Zhangzhou 363000 China

To study the main active components and antioxidant activity in vitro of extracts from Callisia repens , the contents of main active components such as total flavonoids, total anthocyanin and total sugar in the extracts were studied by spectrophotometry. The components and content of 18 kinds of metals were determined by ICP-MS mass spectrometry. Finally, the oxidative activity of the extract was evaluated by spectrophotometry. Results showed that the content of flavonoids, the total protein, the total sugar and the total anthocyanin in C. repens extract powder were 2.04%, 1.83%, 55.2% and 7.2%, respectively. Beneficial trace elements such as Ca, Mn, Mg in C. repens extracts were higher, while harmful heavy metals such as Pb, Hg, Ag, Co, Ge were very tiny or not detected at all. The IC 50 of C. repens was 0.265 mg/mL for scavenging DPPH·, and 1.16 mg/mL for scavenging ·OH free radical, the total reducing power of 1 mg extract was equivalent to that of 39 μg of Vc, and the extract showed no regular chelating power to ferrous ions. In conclusion, C. repens extracts have high content of natural active components, but extremely low content of the harmful heavy metals, and C. repens extract has good antioxidant capacity. Its antioxidant activity is realized by a variety of active factors through a synergistic mechanism. Thus, C. repens extract has great potential for developing into functional foods.

关键词： Callisia repens Antioxidant activity Total flavonoids Total anthocyanin Heavy metal

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白三叶根系分泌物对5种草坪草的生化效应

草地学报 2014年第1期22卷 158-165页

作者：余婷温艳斌孟焕文刘同金赵慧玲程智慧西北农林科技大学林学院陕西杨凌712100 西北农林科技大学园艺学院陕西杨凌712100

为探讨根系分泌物在白三叶(Trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea ... 详细信息

为探讨根系分泌物在白三叶(Trifolium repens L.)入侵禾本科草坪过程中的生化效应,采用水培法收集其根系分泌物,检测不同浓度白三叶根系分泌物对多年生黑麦草(Lolium perenne L.)、紫羊茅(FestucarubraL.)、高羊茅(Festuca arundinacea Schreb.)、草地早熟禾(Poapratensis L.)和匍匐翦股颖(Agrostis stolonifera L.)叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量以及根系活力的影响。结果表明:白三叶根系分泌物对5种草坪草上述生理生化指标均具有浓度效应。在低浓度下(0.002 g·mL^(-1))对SOD和CAT活性具有促进效应,在高浓度下(0.05 g·mL^(-1))则具有抑制效应。5种受体的POD活性和MDA含量均随白三叶根系分泌物浓度增大而升高,根系活力则随着其浓度增大而降低。因此,白三叶根系分泌物可引起受体植物对逆境的响应,5种草坪草化感效应的生化机制可能是分泌物中化感物质通过改变受体幼苗体内保护酶活性及脂膜过氧化来表现的。

关键词：白三叶根系分泌物草坪草化感作用生化指标 Trifolium repens L

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白三叶遗传转化体系的探讨

西南农业学报 2011年第3期24卷 1231-1233页

作者：张边江陈全战周泉澄华春南京晓庄学院江苏南京211171

将白三叶Trifolium repens L.无菌种子吸胀15 h后,制备子叶分别放在含不同浓度配比激素的培养基上培养,同时测定白三叶子叶对卡那霉素(Kam)、头孢霉素(Cef)和羧苄青霉素(Carb)的敏感性,探索出适合子叶生长成苗的一套比较完整的体系。结... 详细信息

将白三叶Trifolium repens L.无菌种子吸胀15 h后,制备子叶分别放在含不同浓度配比激素的培养基上培养,同时测定白三叶子叶对卡那霉素(Kam)、头孢霉素(Cef)和羧苄青霉素(Carb)的敏感性,探索出适合子叶生长成苗的一套比较完整的体系。结果表明,用于白三叶子叶遗传转化的培养基为MS+30 g/L蔗糖+8 g/L琼脂+0.1 mg/L NAA+0.5 mg/L6-BA+20 mg/L Kam+250 mg/L Carb。抗性植株最佳生根培养基为MS+15 g/L蔗糖+8 g/L琼脂+0.2 mg/L NAA。通过本实验成功确立了白三叶遗传转化体系,为下一步转基因实验奠定了基础。

关键词：白三叶(Trifolium repens L.) 子叶遗传转化

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过塘蛇药材中没食子酸含量分析

湖北农业科学 2015年第14期54卷 3508-3509,3512页

作者：李兵陈建惠卢汝梅农梅冬钟江广西中医药大学南宁530200 广西中医药大学第一附属医院南宁530012

为研究过塘蛇（Jussiaea repens L.）药材的质量控制技术,分别采用薄层色谱和液相色谱法分析了过塘蛇样品中活性成分没食子酸含量。结果表明,薄层色谱法可半定量分析其没食子酸含量,液相色谱法可定量分析没食子酸,其进样量在0.12-0.86μ... 详细信息

为研究过塘蛇（Jussiaea repens L.）药材的质量控制技术,分别采用薄层色谱和液相色谱法分析了过塘蛇样品中活性成分没食子酸含量。结果表明,薄层色谱法可半定量分析其没食子酸含量,液相色谱法可定量分析没食子酸,其进样量在0.12-0.86μg之间,与峰面积呈良好线性关系（r=0.999 9）,平均回收率为97.87%（RSD=0.70%,n=6）,10份样品中的没食子酸含量为0.48-2.21 mg/g。该方法简单可行、重现性好,可用于过塘蛇药材的质量控制。

关键词：过塘蛇(Jussiaea repens L.) 没食子酸药材质量薄层色谱液相色谱

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丛枝菌根真菌群落对白三叶草生长的影响

生态学报 2010年第6期30卷 1456-1462页

作者：王晓英王冬梅陈保冬黄益宗王幼珊北京林业大学水土保持学院水土保持与荒漠化防治教育部重点实验室北京100083 中国科学院生态环境研究中心北京100085 北京市农林科学院植物营养与资源研究所北京100089

不同施肥处理影响AMF(Arbuscular mycorrhizal fungi)群体结构,然而不同AMF群体结构对植物的生长以及养分吸收的影响尚未见报道,试验利用盆栽实验研究了7种不同来源的丛枝菌根真菌(AMF)群落对白三叶草生长和N、P、K以及微量元素Cu、Zn... 详细信息

不同施肥处理影响AMF(Arbuscular mycorrhizal fungi)群体结构,然而不同AMF群体结构对植物的生长以及养分吸收的影响尚未见报道,试验利用盆栽实验研究了7种不同来源的丛枝菌根真菌(AMF)群落对白三叶草生长和N、P、K以及微量元素Cu、Zn、Mn的吸收的影响。7种AMF群落分离自长期定位施肥试验地,分别为NPK、OM、CK、1/2OM、NP、NK和PK。每年施肥量是300kg N/hm2,135kg P2O5/hm2,300kg K2O/hm2。有机肥处理的N、P、K养分量与试验地NPK处理含量相同,原料以粉碎的麦秆为主,加上适量的大豆饼和棉仁饼,有机肥经堆制发酵后施用。试验土壤采用封丘试验地土壤,经灭菌处理。试验结果表明,接种不同AMF群落均能促进三叶草的生长,对养分吸收则表现不同。分离自CK试验地的AMF群落对三叶草侵染率显著低于其它6种AMF群落。分离自1/2OM和OM试验地的AMF群落较分离自NPK、CK、NP和NK的AMF群落显著促进了三叶草对P的吸收;各种AMF群落都促进了对N和K的吸收;分离自OM、CK、1/2OM、NP、NK试验地的降低了三叶草植株N含量;分离自NPK试验地的AMF群落提高了三叶草植物K含量;对于Cu、Zn、Mn元素的吸收,不同处理存在较大的差异。AMF群落对三叶草生长以及养分吸收贡献不同,这与不同施肥管理下不同AMF群落的优势种属的侵染率、养分转化以及菌丝发育及分布有关。

关键词：丛枝菌根真菌群落施肥白三叶草(Trifolium repens L.) 生长效应

Development, Reproduction and Body Size Variation of Aphis glycines Matsumura Fed on Different Plants

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Journal of Northeast Agricultural University(English Edition) 2019年第3期26卷 27-35页

作者： Liu Jian Wang Su-ji Bai Bing Gao Bo Fan Yan-jie College of Agriculture Northeast Agricultural University

Soybean aphid, Aphis glycines Matsumura is one of the most important pests in soybean. Life cycle of A. glycines is characterized as heteroecious and holocyclic. The primary hosts of A. glycines are Rhamnus spp. and the secondary hosts include cultivated soybean, Glycine max(L.) Merr, and wild soybean, Glycine soja Sieb.& Zucc. In this study, A. glycines were fed on Trifolium repens L. and Metaplexis japonica(Thunb.) Makino for three generations and their development, reproduction and body sizes were studied. These data were compared to the control fed on the known hosts, G. max and G. soja. These newly deposited offspring by the 3 rd generation were transferred back onto G. max and these differences in their development, reproduction and body sizes were also studied. It showed that A. glycines all could survive, develop and reproduce well, when they were fed on T. repens and M. japonica for three generations, but there were significant differences in their nymph stage, adult longevity and fecundity, life table parameters and body sizes. When these offspring were transferred back onto G. max which were newly deposited by the 3 rd generation aphids fed on T. repens and M. japonica, they could survive, develop and reproduce well, but there were significant differences in their nymph stage, adult longevity, intrinsic rate of increase and body sizes. It provided important information for studies on host adaptability of A. glycines on T. repens and M. japonica and to make clear the potential that this aphid involved into host biotypes on these plants.

关键词： Aphis glycines Matsumura Trifolium repens L. Metaplexis japonica (Thunb.) Makino

高效表达黑曲霉PhyA基因改善白三叶草对有机态磷的利用

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作物学报 2007年第2期33卷 250-255页

作者：韩胜芳谷俊涛肖凯河北农业大学农学院

利用子叶下胚轴为外植体,通过农杆菌(Agrobacterium tumefaciens)Ti质粒介导遗传转化途径,建立了高效表达黑曲霉PhyA基因的白三叶草转基因系。PCR和Southern印迹结果表明,PhyA基因已整合到转基因植株的基因组中。Northern印迹结果表明,... 详细信息

利用子叶下胚轴为外植体,通过农杆菌(Agrobacterium tumefaciens)Ti质粒介导遗传转化途径,建立了高效表达黑曲霉PhyA基因的白三叶草转基因系。PCR和Southern印迹结果表明,PhyA基因已整合到转基因植株的基因组中。Northern印迹结果表明,部分转基因系中的PhyA基因具有高表达水平。此外,PhyA基因表达的植酸酶在Patatin信号肽的引导下具有分泌至细胞间隙和根际的能力。与未转基因对照相比,在以植酸盐为唯一磷素供源的条件下,高表达PhyA的转基因系植株的磷素累积量、鲜重和干重显著增加。证明外源转化黑曲霉PhyA基因能显著增强白三叶草利用有机态磷的能力。

关键词：三叶草(Trifolium repens L.) 遗传转化 PhyA基因有机态磷利用