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Repair of Sheep Metatarsus Defects by Using Tissue-engineering Technique

Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第1期25卷 62-67页

作者：李章华杨翼王常勇夏仁云张玉富赵强廖文王永红卢建熙 Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Tissue Engineering Research Center of Academy of Military Medical Sciences Beijing 100085 China Wuhan Institute of Physical Education Wuhan 430079 China Tissue Engineering Research Center of Academy of Military Medical Sciences Beijing 100085 China Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Tissue-engineering bone with porous β-tricalcium phosphate (β-TCP) ceramic and autologous bone marrow mesenchymal stem cells (MSC) was constructed and the effect of this composite on healing of segmental bone defects was investigated. 10-15 ml bone marrow aspirates were harvested from the iliac crest of sheep, and enriched for MSC by density gradient centrifugation over a Percoll cushion (1.073 g/ml). After cultured and proliferated, tissue-engineering bones were constructed with these cells seeded onto porous β-TCP, and then the constructs were implanted in 8 sheep left metatarsus defect (25 mm in length) as experimental group. Porous β-TCP only were implanted to bridge same size and position defects in 8 sheep as control group, and 25 mm segmental bone defects of left metatarsus were left empty in 4 sheep as blank group. Sheep were sacrificed on the 6th, 12th, and 24th week postoperatively and the implants samples were examined by radiograph, histology, and biomechanical test. The 4 sheep in blank group were sacrificed on the 24th week postoperatively. The results showed that new bone tissues were observed either radiographic or histologically at the defects of experimental group as early as 6th week postoperatively, but not in control group, and osteoid tissue, woven bone and lamellar bone occurred earlier than in control group in which the bone defects were repaired in “creep substitution” way, because of the new bone formed in direct manner without progression through a cartilaginous intermediate. At the 24th week, radiographs and biomechanical test revealed an almost complete repair of the defect of experimental group, only partly in control group. The bone defects in blank group were non-healing at the 24th week. It was concluded that engineering bones constructed with porous β-TCP and autologous MSC were capable of repairing segmental bone defects in sheep metatarsus beyond “creep substitution” way and making it healed earlier. Porous β-TCP being constituted

关键词： tissue-engineering bone bone defect regenerated new bone

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