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Fine Structure of regenerated Silk Fibroin Solids

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Journal of Donghua University(English Edition) 2002年第3期19卷 1-6页

作者：李明忠吴徵宇箕浦宪彦严灏景 Coll. of Mat. Eng. Suzhou Univ. Suzhou 215021 China Nanoarchitectonics Res. Cent. Natl. Inst. of Adv. Indust. Sci. Ibaraki 305-8565 Japan Coll. of Textiles Dong Hua Univ. Shanghai 200051 China

The fibroin solids (membrane and gel) were prepared from regenerated silk fibroin solution in different ways. The structure of the fibroin solids and its change during storage were studied. The results indicated that the structure of fibroin membrane air-dried at 30℃ or freeze dried at a freezing temperature of -20-4℃ was the coexistence of amorphous and silkⅠstructure. The amorphous region could partly be transformed into silkⅠstructure under room temperature and humidity. The structure of porous silk fibroin membrane was the coexistence of amorphous and small part of silkⅡ, if the aqueous fibroin was freeze dried within the temperature range of -80℃ to -20℃, and the amorphous partly transformed into silkⅠ during storage. The structure of fibroin gel naturally forming when kept at room temperature was coexistent of amorphous and silkⅡ. The content of Gly and Ala was high in the surface membrane of aqueous fibroin and its structure was mostly silkⅠ and silkⅡwith high crystallinity.

关键词： silk fibroin, regenerated fibroin, membrane, fine structure.

Conformational Transitions and Self-assembly of regenerated Spider Silk Protein in Water

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Journal of Donghua University(English Edition) 2014年第5期31卷 668-674页

作者： ZHANG Ye-mei WANG Zhe PAN Zhi-juan College of Textile and Clothing Engineering Soochow University Suzhou 21502i Ctffna" National Engineering Laboratory for Modern Silk Soochow University Suzhou 215123 China

Spider silk, relying on its exceptional mechanical properties, has attracted extensive attention throughout the world. The structure of a material can influence its mechanical properties. Investigation of the structure of spider silk includes amino acid composition, molecular structure, self-assembly, and crystallization, among other characteristics. Herein, the effects of concentration, time, alkali metal ions （Na^＋and K ^＋） and pH on the conformational transition and self-assembly of regenerated Ornithoctonus huwena spider dragline silk protein （spidroin） in water were investigated using circular dichroism （CD） and atomic force microScopy （AFM）. Spidroin concentration, time and Na ＋ ions slightly influenced the conformational transition of spidroin molecules. However, K ＋ ions and pH induced the formation of a β- sheet structure. Increasing spidroin concentration or time increased the aggregation of spidroin and enhanced the formation of nanoffiaments. K ~ ions enhanced the serf-assembly of spidroin into nanofilaments. The self-assembled nanofilaments appeared at a pH of approximately 6. 11. Both lower and higher pH induced aggregation. At a lower pH, the aggregation was composed of nanopartides, whereas higher pH induced the aggregation of nanoffiaments, likely from the synergistic effect of Na ^＋ ions and pH.

关键词： regenerated spider silk conformational transition self-assembly

SOYBEAN LEAF CULTURE in vitro AND INDUCTION OF regenerated PLANTS

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Chinese Science Bulletin 1985年第4期30卷 527-533页

作者：杨振棠陈泽光刘志东张增明 Jilin Institute of Agricultural Science Jilin Institute of Agricultural Sciencehe techniques used in the tissue culture of somatic plant cells have great significance for the acceleration of breeding of choice varieties conservation of the good resources of germplasm selection of useful mutants and overcoming some seldom resolvable difficulties in conventional breeding as well as the genetic operations of soybeans.

The techniques used in the tissue culture of somatic plant cells have great significance for the acceleration of breeding of choice varieties, conservation of the good resources of germplasm, selection of useful mutants, and overcoming some seldom resolvable difficulties in conventional breeding as well as the genetic operations of soybeans.

关键词： breeding germplasm soybean seldom somatic shoot acceleration anther operations regenerated

DIVISION OF regenerated CELLS FROM MESOPHYLL PROTOPLAST OF BARLEY

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Chinese Science Bulletin 1980年第7期25卷 609-611页

作者：颜秋生李向辉 Institute of Ceneties Academia Sinica

The progress of plant protoplast culture and somatic hybridization technique has demonstrated a significant potential in exploring new approaches to plant improvement as well as in genetic engineering studies. Many researchers concentrated their effort on the culture of important crops, especially cereals. Protoplast cultures of cereals have been reported[1-6], yet no plant regeneration has ever been obtained, and up to date no regularly sustained division ever detected in cultured protoplasts from cereal mesophylls. In this paper we report the results on the division of regenerated cell from mesophyll protoplasts of barley.

关键词： sustained somatic regularly exploring cultured regeneration crops regenerated cereal concentrated

Unexpected Postoperative Paraplegia after Thoracotomy in Lung Cancer: Incidental Migration of Oxidized regenerated Cellulose Used for Hemostasis of Intercostal Space Bleeding

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Surgical Science 2017年第8期8卷 365-374页

作者： Takanori Ayabe Masaki Tomita Shigeko Shimizu Etsuko Yokoyama Manabu Okumura Koichiro Itai Kunihide Nakamura Division of Thoracic and Breast Surgery Department of Surgery Faculty of Medicine University of Miyazaki Miyazaki Japan Division of Patient Safety Management University of Miyazaki Hospital Miyazaki Japan Division of Clinical Ethics University of Miyazaki Hospital Miyazaki Japan Division of Cardiovascular Surgery Department of Surgery Faculty of Medicine University of Miyazaki Miyazaki Japan

Background: We experienced a very rare complication, that is, an unexpected postoperative paraplegia due to the incidental migration of oxidized regenerated cellulose used for hemostasis of intercostal space bleeding. Patients and Methods: The objective is to analyze the cause and to take measures against the very rare complication from an empirical analysis and the literature. For a 78-year-old male with suspected lung cancer in the right upper lobe (S1), a thoracotomy was performed. For hemostasis of the bleeding from the 5th intercostal thoracotomy space, we used and placed oxidized regenerated cellulose at the continuous oozing bleeding sites. On the 3rd postoperative day, paralysis beneath thoracic vertebrae level 6 was observed. Immediate computed-tomographic (CT) scanning and magnetic resonance imaging (MRI) displayed a 17 × 9 × 14 mm epidural hematoma in the spinal canal at level 5 of the thoracic vertebrae. An emergent laminectomy for the thoracic vertebra was performed to remove the oxidative cellulose and haematoma, and the compression was released. The paraplegia gradually began to recover and maintain a standing position. After 1 year from the event, the patient can walk by himself with a crutch. Results: The causes were that the oxidative cellulose materials were used for the intercostal bleeding at the open thoracotomy. The migration of the oxidative cellulose materials into the epidural space and into thoracic spinal canal through the intervertebral foramen, or gradual penetration of the oxidative cellulose materials into the spinal canal due to respiratory costal movement. As a measurement of prevention, the hemostat materials should be completely removed after finishing of the hemostasis. In the case of a difficult hemostasis, consultation of an orthopedist or neurosurgeon to perform the appropriate hemostasis in good cooperation is required. Conclusion: If postoperative paraplegia is suspected, immediate CT scanning and/or MRI examination would be

关键词： Lung Cancer Incident Oxidized regenerated Cellulose Migration Thoracotomy Intercostal Space Bleeding Paraplegia

The National Development and Reform Commission issues program for regenerated energies in the 11th five-year period

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中国汽车（英文版） 2008年第4期 3-4页

By 2010, the amount of regenerated energies being used will be equal to 300 million tons of coal, or nearly double the amount in 2005, according to the program issued on March 18, 2008.

关键词： regenerated issued county demonstration biomass pr

FROM EMG CONTROLLING TO"regenerated FINGER" CONTROLLING ARTIFICIAL HAND-THE RECENT ACHIEVEMENT IN REHABILITATION ENGINEERING

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Chinese Journal of Biomedical Engineering(English Edition) 1997年第3期6卷 151-151页

作者： Hu Tianpei Xu Zhengsong Chee Ben Gao Zhonghua Wu Tongshen(Institute of Rehabilitation Engineering, Shanghai Jiao Tong University, CHINA)

According to the sampling statistics there are over 7 millions limb disabled persons in China from several times of those in the world. For the benefit to the amputees, thousands varies kinds of commencial Products of artificial hands, by scientific promotion have ben developed in the recent half century. Among those products the EMG controlling artificial upper limb brings the hope to the amputees. The later are now used widely form the earlest one in *** 1978, Shanghai Jiao Tong University began to study and develop the EMG controlling artificial band mounted to amputes over thousand disabled during a couple of recent *** risidual muscles of an amputee are the signal source of the artificial hand controlled by EMG. The evoked EMG which can transmit the brain moving information is one of the bioelectricities from human body. The EMG signal, accumulated at skin surface with surface electrodes, passing through filtering and amplifying circuits controls the movement-this is the main principle of EMG controlling artificial ***, owing to the weakness of EMG (μv) and a group of muscles information plus the strong turbulence of electric field (v), the integrated EMG can’t completely reflect the brain moving act and will influence the accuracy of EMG-hand, especially those of the multi-multi-degree of *** 1978, only 57% of the controlling aaccuracy of artificial hand with 3-degree of freedom could be reached by the Herbert’s research. In 80’s, the controlling accuracy raised up to 72% by Denning’s new method. Up to now, the accuracy is still not ideal, eventhrogh the Hi-tech of using pattern-recognitionand artincial neuro-net work. The electronic artificial hand will be considered successful and practical only with the moving accuracy more than 95%.Some research by using implant electrode for detecting the neuro-information or EEG controllingmethod met also dimculties for raising the accuracy of artificial *** breaking througll the threshold of

关键词： Toe to stump regenerated finger Electronic artificial hand EMG controlling artifical hand

Adsorption of Methylene Blue from Aqueous Solution onto Hydrochloric Acid-modified Rectorite

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Journal of Wuhan University of Technology(Materials Science) 2011年第5期26卷 817-822页

作者：张高科 School of Resources and Environmental Engineering Wuhan University of Technology

H＋-rectorite clay,which was prepared by modifying the raw rectorite with 10% hydrochloric acid at 60 °C for 24 h,was used as an absorbent for removal of methyl blue （MB） from aqueous *** morphology and the structure and crystallinity of the pristine rectorite and the H＋-rectorite were characterized by scanning electron microscopy （SEM） technique and X-ray diffraction （XRD） technique,*** results showed that the H＋-rectorite exhibited high adsorption ability than the raw rectorite,and it was found that the removal percentage of MB increased with increasing in adsorbents dose,whereas the adsorption amount qe （mg/g） *** equilibrium was attained within 30 min in adsorption process,and the maximum adsorption capacity of H＋-rectorite for methylene blue reached as high as 37 mg/***,the effect of temperature on the adsorption of MB with H＋-rectorite was investigated and the equilibrium data were well fitted to Freundlich *** H＋-rectorite absorbent saturated with MB can be regenerated by calcinating at 400 °C for 2 h and the regenerated absorbent still showed higher percentage removal of MB.

关键词： rectorite modified MB adsorption kinetics regenerated

Fabrication of Superhydrophobic Surfaces of Cellulose Sheets by Creating Biomimic Structures

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Chemical Research in Chinese Universities 2014年第6期30卷 1071-1076页

作者： ZHAO Wei WANG Xiaolin LIMing XI Wang LIU Mingrui Sinopec Fushun Research Institute of Petroleum and Petrochemicals Fushun 113001 P R. China

The biodegradable superhydrophobic cellulose sheets were fabricated by simple dissolution, controllable crystallization, coagulation, and Teflon dip-coat. The surface morphology of the superhydrophobic regenerated cel- lulose sheets is similar to that of the natural lotus leaves consisting of hierarchical micro/nano structures. The pre- pared cellulose sheets exhibit a remarkable suoerhvdroohobicity and satisfactory long-term chemical stabilitv.

关键词： Superhydrophobic cellulose sheet Hierarchical structure regenerated Biodegradable Chemical stability