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The Deep Thioredoxome in Chlamydomonas reinhardtii： New Insights into redox regulation

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Molecular Plant 2017年第8期10卷 1107-1125页

作者： Maria Esther Perez-Perez Adeline Mauries Alexandre Maes Nicolas J. Tourasse Marion Hamon Stephane D. Lemaire Christophe H. Marchand Institut de Biologie Physico-Chimique UMR8226 CNRS Sorbonne Universit6s UPMC Univ Paris 06 13 rue Pierre et Marie Curie 75005 Paris France Institut de Biologie Physico-Chimique Plateforme de Proteomique FRC550 CNRS 13 rue Pierre et Marie Curie 75005 Paris France Instituto de Bioquimica Vegetal y Fotosintesis Consejo Superior de Investigaciones Cientificas (CSIC)-Universidad de Sevilla Seville Spain ARNA Laboratory INSERM U1212 CNRS UMR5320 Universite Bordeaux 2 Bordeaux France

Thiol-based redox post-translational modifications have emerged as important mechanisms of signaling and regulation in all organisms, and thioredoxin plays a key role by controlling the thiol-disulfide status of target proteins. Recent redox proteomic studies revealed hundreds of proteins regulated by glutathio- nylation and nitrosylation in the unicellular green alga Chlamydomonas reinhardtii, while much less is known about the thioredoxin interactome in this organism. By combining qualitative and quantitative proteomic analyses, we have comprehensively investigated the Chlamydomonas thioredoxome and 1188 targets have been identified. They participate in a wide range of metabolic pathways and cellular pro- cesses. This study broadens not only the redox regulation to new enzymes involved in well-known thiore- doxin-regulated metabolic pathways but also sheds light on cellular processes for which data supporting redox regulation are scarce （aromatic amino acid biosynthesis, nuclear transport, etc）. Moreover, we char- acterized 1052 thioredoxin-dependent regulatory sites and showed that these data constitute a valuable resource for future functional studies in Chlamydomonas. By comparing this thioredoxome with proteomic data for glutathionylation and nitrosylation at the protein and cysteine levels, this work confirms the existence of a complex redox regulation network in Chlamydomonas and provides evidence of a tremendous selectivity of redox post-translational modifications for specific cysteine residues.

关键词： Chlamydomonas reinhardtii disulfide bond isotope-coded affinity tag redox proteomics redox regulation thioredoxin targets

Accumulation of Polyphenolic Compounds and Osmolytes under Dehydration Stress and Their Implication in redox regulation in Four Indigenous Aromatic Rice Cultivars

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Rice science 2020年第4期27卷 329-344页

作者： Nivedita DEY Soumen BHATTACHARJEE Plant Physiology and Biochemistry Research Laboratory/University Grants Commission Centre for Advanced Study Department of BotanyUniversity of BurdwanBurdwan 713104West BengalIndia

Present work was undertaken to screen some drought tolerant indigenous aromatic rice cultivars(IARCs),commonly cultivated in West Bengal,India,based on their capacity to produce osmolytes,redox-sensitive phenolic acids and flavonoids,as contrivances for redox-regulation under drought *** glycol induced post imbibitional dehydration stress mediated changes in redox regulatory properties of the germinating seeds of the four IARCs(Jamainadu,Tulaipanji,Sitabhog,Badshabhog),which were assessed in terms of changes in prooxidant accumulation(in-situ localization of reactive oxygen species(ROS)by confocal microscopy,DCFDA(2′,7′-dichlorofluorescin diacetate)oxidation,O2-and H2O2 accumulation),cumulative antioxidative defense(radical scavenging property and total thiol content),ROS scavenging phenolic acids(gallic acid,protocatechuic acid,gentisic acid,para-hydroxy benzoic acid,chlorogenic acid,caffeic acid,syringic acid,salicylic acid,sinapic acid and p-coumaric acid)and flavonoids(catechin,naringin,rutin,quercetin,kaempferol,myricetin and apigenin).The capability of germinating seeds to accumulate osmolytes(like glycinebetaine,proline,soluble carbohydrates and K+ion)and polyphenolic compounds was also correlated with their corresponding redox status and redox biomarkers(conjugated diene,hydroperoxide,thiobarbituric acid reactive substances and free carbonyl content)produced under the same *** results in general showed that accumulation of osmolytes along with the redox-sensitive phenolics and flavonoids conferred the ability to maintain the redox homeostasis under drought stress for the tolerant IARCs(Badshabhog and Tulaipanji).

关键词： aromatic rice drought polyphenolic compounds osmolytes redox regulation

redox-Dependent regulation of the Stress-Induced Zinc-Finger Protein SAP12 in Arabidopsis thaliana

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Molecular Plant 2009年第2期2卷 357-367页

作者： Elke Stroeher Xin-Jia Wang Nils Roloff Peter Klein Arne Husemann Karl-Josef Dietz Biochemistry and Physiology of Plants Bielefeld University 33501 Bielefeld Germany

The stress-associated protein SAP12 belongs to the stress-associated protein （SAP） family with 14 members in Arabidopsis thaliana. SAP12 contains two AN1 zinc fingers and was identified in diagonal 2D redox SDS-PAGE as a protein undergoing major redox-dependent conformational changes. Its transcript was strongly induced under cold and salt stress in a time-dependent manner similar to SAP10, with high levels after 6 h and decreasing levels after 24 and 48 h. The tran- script regulation resembled those of the stress marker peroxiredoxin PrxllD at 24 and 48 h. Recombinant SAP12 protein showed redox-dependent changes in quaternary structure as visualized by altered electrophoretic mobility in non-reducing SDS polyacrylamide gel electrophoresis. The oxidized oligomer was reduced by high dithiothreitol concentrations, and also by E. coli thioredoxin TrxA with low dithiothreitol （DTF） concentrations or NADPH plus NADPH-dependent thioredoxin reductase. From Western blots, the SAP12 protein amount was estimated to be in the range of 0.5 ngμg^-1 leaf protein. SAP12 protein decreased under salt and cold stress. These data suggest a redox state-linked function of SAP12 in plant cells particularly under cold and salt stress.

关键词： abiotic/environmental stress cold acclimation cell signaling gene expression Arabidopsis A20 and AN1 Zinc finger domains redox regulation stress associated protein thioredoxin.

Quantitative proteomics reveals redox-based functional regulation of photosynthesis under fluctuating light in plants

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Journal of Integrative Plant Biology 2022年第11期64卷 2168-2186页

作者： Qi Chen Yixian Xiao Yu Ming Rong Peng Jiliang Hu Hong-Bin Wang Hong-Lei Jin Institute of Medical Plant Physiology and Ecology School of Pharmaceutical SciencesGuangzhou University of Chinese MedicineGuangzhou 510006China

Photosynthesis involves a series of redox reactions and is the major source of reactive oxygen species in plant *** light(FL) levels,which occur commonly in natural environments,affect photosynthesis;however,little is known about the specific effects of FL on the redox regulation of ***,we performed global quantitative mapping of the Arabidopsis thaliana cysteine thiol redox proteome under constant light and FL *** identified8857 redox-switched thiols in 4350 proteins,and1501 proteins that are differentially modified depending on light ***,proteins related to photosynthesis,especially photosystem I(PSI),are operational thiol-switching *** of wild-type *** to FL resulted in decreased PSI abundance,stability,and ***,in response to PSI photodamage,more of the PSI assembly factor PSA3 dynamically switches to the reduced ***,the Cys199 and Cys200 sites in PSA3 are necessary for its full ***,thioredoxin m(Trx m) proteins play roles in redox switching of PSA3,and are required for PSI activity and *** study thus reveals a mechanism for redox-based regulation of PSI under FL,and provides insight into the dynamic acclimation of photosynthesis in a changing environment.

关键词： fluctuating light photosystem I photosynthesis redox regulation thioredoxin

regulation of Calvin-Benson cycle enzymes under high temperature stress

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aBIOTECH 2022年第1期3卷 65-77页

作者： Juan-Hua Chen Ming Tang Xue-Qi Jin Han Li Li-Sha Chen Qing Long Wang Ai-Zhen Sun Yin Yi Fang-Qing Guo National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences Institute of Plant Physiology and EcologyChinese Academy of Sciences300 Fenglin RoadShanghai 200032China Key Laboratory of Plant Physiology and Developmental regulation School of Life SciencesGuizhou Normal UniversityGuiyang 550001GuizhouChina Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China Guizhou Normal UniversityGuiyang 550001GuizhouChina University of Chinese Academy of Sciences Beijing 100049China

The Calvin Benson cycle(CBC)consists of three critical processes,including fixation of CO_(2) by Rubisco,reduction of 3-phosphoglycerate(3PGA)to triose phosphate(triose-P)with NADPH and ATP generated by the light reactions,and regeneration of ribulose 1,5-bisphosphate(RuBP)from *** activ-ities of photosynthesis-related proteins,mainly from the CBC,were found more significantly affected and regulated in plants challenged with high temperature stress,incuding Rubisco,Rubisco activase(RCA) and the enzymes involved in RuBP regeneration,such as sedoheptulose-1,7-bisphosphatase(SBPase).Over the past years,the regulatory mechanism of CBC,especially for redox-regulation,has attracted major interest,because balancing flux at the various enzymatic reactions and maintaining metabolite levels in a range are of critical importance for the optimal operation of CBC under high temperature stress,providing insights into the genetic manipulation of ***,we summarize recent progress regarding the identification of various layers of regulation point to the key enzymes of CBC for acclimation to environmental temperature changes along with open questions are also discussed.

关键词： Calvin-Benson cycle High temperature stress redox regulation Rubisco Rubisco activase SBPase CO_(2)fixation

Methionine oxidation and reduction of the ethylene signaling component MaEIL9 are involved in banana fruit ripening

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Journal of Integrative Plant Biology 2023年第1期65卷 150-166页

作者： Lisha Zhu Lin Chen Chaojie Wu Wei Shan Danling Cai Zengxiang Lin Wei Wei Jianye Chen Wangjin Lu Jianfei Kuang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation Ministry of EducationCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural AffairsCollege of Horticulture South China Agricultural UniversityGuangzhou 510642 China

The ethylene insensitive 3/ethylene insensitive3-like(EIN3/EIL)plays an indispensable role in fruit ***,the regulatory mechanism that links post-translational modification of EIN3/EIL to fruit ripening is largely ***,we studied the expression of 13 MaE IL genes during banana fruit ripening,among which MaE IL9 displayed higher enhancement particularly in the ripening *** with its transcript pattern,abundance of MaE IL9 protein gradually increased during the ripening process,with maximal enhancement in the *** affinity purification(DAP)-seq analysis revealed that MaE IL9 directly targets a subset of genes related to fruit ripening,such as the starch hydrolytic genes MaA MY3D and MaB *** overexpressing MaE IL9 in tomato fruit hastened fruit ripening,whereas transiently silencing this gene in banana fruit retarded the ripening process,supporting a positive role of MaEIL9 in fruit ***,oxidation of methionines(Met-129,Met-130,and Met-282)in MaEIL9 resulted in the loss of its DNA-binding capacity and transcriptional activation ***,we identified MaEIL9 as a potential substrate protein of methionine sulfoxide reductase A MaMsrA4,and oxidation of Met-129,Met-130,and Met-282in MaEIL9 could be restored by ***,our findings reveal a novel regulatory network controlling banana fruit ripening,which involves MaMsrA4-mediated redox regulation of the ethylene signaling component MaEIL9.

关键词： banana EIN3/EIL redox regulation ROS starch degradation

Proteome-wide identification of S-sulfenylated cysteines response to salt stress in Brassica napus root

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Oil Crop Science 2023年第4期8卷 243-251页

作者： Qian Qu Xiaowei Wu Qing Zhou Shaoping Lu Xuan Yao Liang Guo Liangqian Yu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan430070China Yazhouwan National Laboratory Sanya572025China

Reactive oxygen species(ROS)play a key role in a variety of biological processes,such as the perception of abiotic stress,the integration of different environmental signals,and the activation of stress response *** stress could induce an increased ROS accumulation in plants,disrupting intracellular redox homeostasis,leading to posttranslational modifications(PTMs)of specific proteins,and eventually causing adaptive changes in ***,we performed an iodoTMT-based proteomic approach to identify the sulfenylated proteins in *** root responsing to salt ***,1348 sulfenylated sites in 751 proteins were identified and these proteins were widely existed in different cell compartments and *** study revealed that proteins with changed abundance and sulfenylation level in *** root under salt stress were mainly enriched in the biological processes of ion binding,glycolysis,ATP binding,and oxidative stress *** study displays a landscape of sulfenylated proteins response to salt stress in *** root and provides some theoretical support for further understanding of the molecular mechanisms of redox regulation under salt stress in plants.

关键词： IodoTMT Brassica napus root Salt stress Sulfenylation redox regulation

The roles of microRNA in redox metabolism and exercise-mediated adaptation

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Journal of Sport and Health Science 2020年第5期9卷 405-414页

作者： Ferenc Torma Zoltan Gombos Matyas Jokai Istvan Berkes Masaki Takeda Tatsuya Mimura Zsolt Radak Ferenc Gyori Research Center of Molecular Exercise Science University of Physical EducationBudapest 1123Hungary Faculty of Health and Sports Science Doshisha UniversityKyotanabe 610-0394Japan Faculty of Sport and Health Sciences Osaka Sangyo UniversityOsaka 573-1004Japan Faculty of Sport Sciences Waseda UniversityTokorozawaSaitama 359-1192Japan Institute of Physical Education and Sport Science JGYPKUniversity of SzegedSzeged 6726Hungary

MicroRNAs(miRs)are small regulatory RNA transcripts capable of post-transcriptional silencing of mRNA messages by entering a cellular bimolecular apparatus called RNA-induced silencing *** are involved in the regulation of cellular processes producing,eliminating or repairing the damage caused by reactive oxygen species,and they are active players in redox *** mitochondrial biogenesis,function and hypertrophy of skeletal muscle are important adaptive responses to regular *** the present review,we highlight some of the redox-sensitive regulatory roles of miRs.

关键词： Adaptation Exercise MicroRNA Oxidative damage Reactive oxygen species redox regulation

Thioredoxins Play a Crucial Role in Dynamic Acclimation of Photosynthesis in Fluctuating Light

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Molecular Plant 2017年第1期10卷 168-182页

作者： Ina Thormahlen Arkadius Zupok Josephin Rescher Jochen Leger Stefan Weissenberger Julia Groysman Anne Orwat Gilles Chatel-lnnocenti Emmanuelle Issakidis-Bourguet Ute Armbruster Peter Geigenberger Department Biologie I Ludwig-Maximilians-Universitat MQnchen Grosshaderner StraSe 2-4 82152 Martinsried Germany Max-Planck-lnstitut fur Molekulare Pflanzenphysiologie Department 3 Am Muhlenberg 1 14476 Potsdam-Golm Germany Institute of Plant Sciences Paris Saclay (IPS2) UMR 9213/UMR1403 Universite Paris Sud CNRS INRA Universit~ d'Evry Universite Paris Diderot Sorbonne Paris Cite Batiment 630 91405 Orsay France

Sunlight represents the energy source for photosynthesis and plant growth. When growing in the field, plant photosynthesis has to manage strong fluctuations in light intensities. regulation based on the thio- redoxin （Trx） system is believed to ensure light-responsive control of photosynthetic reactions in the chlo- roplast. However, direct evidence for a role of this system in regulating dynamic acclimation of photosyn- thesis in fluctuating conditions is largely lacking. In this report we show that the ferredoxin-dependent Trxs ml and m2 as well as the NADPH-dependent NTRC are both indispensable for photosynthetic acclimation in fluctuating light intensities. Arabidopsis mutants with combined deficiency in Trxs ml and m2 show wild- type growth and photosynthesis under constant light condition, while photosynthetic parameters are strongly modified in rapidly alternating high and low light. Two independent trxmlm2 mutants show lower photosynthetic efficiency in high light, but surprisingly significantly higher photosynthetic efficiency in low light. Our data suggest that a main target of Trx ml and m2 is the NADP-malate dehydrogenase involved in export of excess reductive power from the chloroplast. The decreased photosynthetic efficiency in the high-light peaks may thus be explained by a reduced capacity of the trxm lm2 mutants in the rapid light acti-vation of this enzyme. In the ntrc mutant, dynamic responses of non-photochemical quenching of excita- tion energy and plastoquinone reduction state both were strongly attenuated in fluctuating light intensities, leading to a massive decrease in PSII quantum efficiency and a specific decrease in plant growth under these conditions. This is likely due to the decreased ability of the ntrc mutant to control the stromal NADP（H） redox poise. Taken together, our results indicate that NTRC is indispensable in ensuring the full range of dynamic responses of photosynthesis to optimize photosynthesis and maintain growth in fluc

关键词： arabidopsis chloroplast light signalling NTRC redox regulation thioredoxin