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Land recultivation for refuse dumps of Haizhou Opencast

中国有色金属学会会刊：英文版 2005年第S1期15卷 32-34页

作者： WU Wen-bo~1, WU Qin~2, MA Zhen-li~1, YANG Gui-jun~1 (1. Liaoning Technical University, Fuxin 123000, China 2. Haizhou Open-pit, Fuxin 123001, China)

According to the degree of land cultivation based on the service life of dump, the soil quality and the natural circumstance, the land recultivation region may be diuided into three areas of high-technical agriculture, forestry and livestock farming. After land rebuilding, the vegetation will achieve the standard of farming and forestry to form an integrated harness area of agriculture, forestry and livestock farming. The purposes are to provide a magnitude of employment and enhance the land utilizing capability, as well as to generalize in other minefields in which the social benefit will be significant. Moreover, the recultivation will bring back a green urban to Fuxin City and eliminate a serious source of pollution, therefore the live quality of citizen will be improved.

关键词： land recultivation dump land utilization

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KuiqpickTM:a low cost instrument for tissue microdissection and live single cell collection

KuiqpickTM:a low cost instrument for tissue microdissection ...

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中国神经科学学会第十届全国学术会议

作者： Zhong-Cai Ma Lili C.Kudo Anatol Bragin Stanislav Karsten Neurology David Geffen School of Medicine at UCLA

Objective The current work demonstrates the efficiency of Kuiqpick for brain tissue microdissection with follow-up RNA and protein isolation,dissection of hve brains for neural progenitor cultures,and collection of individual cells from various adherent cell *** Various subanatomical areas of hve,freshly frozen or sucrose-treated mouse brain were dissected,DNA,RNA and proteins were extracted and *** zones were isolated from fresh hve rat brain tissues,and used for neural progenitor cultures(NPC).Individual cells were collected from NPC,SH-SY5Y,CHO and MDA-MB-435 cultures based on morphology or fluorescent *** cells were tested for *** Kuiqpick efficiently collects specific areas of the brain at the cellular *** instrument works in a wide range of parameters and dissects brain sections of various thicknesses ranging from 10 to 500 μ***,fresh frozen and sucrose treated tissues can be used for the dissection of subanatomical regions or even individual *** quality RNA and proteins can be isolated from the collected samples for genomics and proteomics *** employed principle of a gentle vacuum suction has minimal impact on cellular viabihty;therefore,cells collected from cultures or native brain tissues may be used for downstream *** of cells can be performed based on their morphology or fluorescent *** We demonstrated several applications of Kuiqpick in neuroscience research,including(1) brain subanatomical region specific collection for-omics research;(2) collection of brain neurogenic areas for neural progenitor cultures and(3)collection of individual/single cells from in vitro cultures based on their morphology and fluorescent *** low cost of Kuiqpick,its accuracy,ease of use,compatibility with fluorescence,versatility,and compatibility with hve cells and tissues,make it a cell and tissue acquisition system of choice for high qualit

关键词： KuiqpickTM brain microdissection single cell analysis recultivation

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