检索结果-南通市图书馆

Monte Carlo study of B-cell receptor clustering mediated by antigen crosslinking and directed transport

Cellular & Molecular Immunology 2011年第3期8卷 255-264页

作者： A Srinivas Reddy Philippos K Tsourkas Subhadip Raychaudhuri Department of Biomedical Engineering University of California DavisDavisUSA Biophysics Graduate Group University of California DavisDavisUSA Graduate Group in Applied Mathematics University of California DavisDavisUSA

It is known from experiments that in the presence of soluble antigen,B-cell receptors(BCRs)assemble into microclusters and then collect into a macrocluster known as a‘cap’.However,the mechanisms of BCR cluster formation during recognition of soluble antigens remain *** previous work,we demonstrated that effective intrinsic attractions among BCRs can lead to the formation of small microclusters of BCR *** effective intrinsic attractions could be caused by multivalent antigen binding,association with lipid rafts,or other biochemical *** the present study,we have developed and studied a Monte Carlo model of BCR clustering mediated by explicit binding and crosslinking of soluble bivalent *** crosslinking is shown to microcluster BCRs in an affinity-dependent manner and also in a biologically relevant timescale;however,antigen crosslinking alone does not appear to be sufficient for the formation of a single macrocluster of receptor *** show that directed transport of BCRs is needed to drive the formation of large *** constructed a simple model of directed transport,where BCR molecules diffuse towards the largest cluster or towards a random BCR microcluster,which results in a single macrocluster of receptor *** mechanisms for both types of directed transport are compared using network-based *** also develop and use appropriate network measures to analyze the effect of BCR and antigen concentration on BCR clustering,the stability of the formed clusters over time and the size of BCR–antigen crosslinked chains.

关键词： B-cell activation B-cell receptor cell signaling Monte Carlo simulation receptor clustering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

clustering of surface NMDA receptors is mainly mediated by the C-terminus of GluN2A in cultured rat hippocampal neurons

引用

Neuroscience Bulletin 2014年第4期30卷 655-666页

作者： Ying-Gang Yan Jie Zhang Shu-Jun Xu Jian-Hong Luo Shuang Qiu Wei Wang Department of Neurobiology Key Laboratory of Medical Neurobiology of the Ministry of Health of ChinaZhejiang Province Key Laboratory of NeurobiologyZhejiang University School of Medicine

N-methyI-D-aspartate receptors （NMDARs） containing different GluN2 subunits play distinct roles in synaptic plasticity. Such differences may not only be determined by the channel properties, but also by differential surface distribution and synaptic localization. In the present study, using a Cy3-conjugated Fab fragment of the GFP antibody to label surface-located GluN2 subunits tagged with GFP at the N-terminus, we observed the membrane distribution patterns of GluN2A- or GluN2B-containing NMDARs in cultured rat hippocampal neurons. We found that surface NMDARs containing GluN2A, but not those containing GluN2B, were inclined to cluster at DIV7. Swapping the carboxyl termini of the GluN2 subunits completely reversed these distribution patterns. In addition, surface NMDARs containing GluN2A were preferentially associated with PSD-95. Taken together, the results of our study suggest that the clustering distribution of GluN2A- containing NMDARs is determined by the GluN2A C-terminus, and its interaction with PSD-95 plays an important role in this process.

关键词： NMDA receptors GluN2A GluN2B PSD-95 receptor clustering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：