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基于叶绿体基因rbcl和trnL-F序列的河北省野生苔草属亲缘关系分析

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草地学报 2024年第09期 2728-2736页

作者：周欣莹董彦娜邱玉鹏孙浩男刘亚伟李艳刘冬云河北农业大学园林与旅游学院石家庄信息工程职业学院

为研究河北省野生苔草属(Carex)植物的亲缘关系，本研究以43种河北省野生苔草及国外引种的27份苔草种质为试验材料，克隆了叶绿体基因rbcl和trnL-F序列，进行遗传多样性及亲缘关系分析，并构建了系统发育树。结果表明，从70份苔草属植... 详细信息

为研究河北省野生苔草属(Carex)植物的亲缘关系，本研究以43种河北省野生苔草及国外引种的27份苔草种质为试验材料，克隆了叶绿体基因rbcl和trnL-F序列，进行遗传多样性及亲缘关系分析，并构建了系统发育树。结果表明，从70份苔草属植物中成功克隆到的rbcl和trnL-F序列数量分别为67条、60条，测序成功率分别为95.7%、85.7%,经正反序列拼接对齐获得序列长度分别为716 bp、976 bp,并上传至NCBI获得登录号。二者合并得到59条序列，长度为1692 bp。rbcl,trnL-F以及合并序列分别定义了43,56,58个单倍型。基于合并序列的59份种质间遗传距离为0～0.0421,平均遗传距离为0.0142,整体划分为3大类，与形态学分类基本相符。trnL-F序列有望开发为DNA条形码，以期为苔草属的分类鉴定、系统发育等研究提供依据。

关键词：苔草属 rbcl trnL-F 亲缘关系遗传多样性

濒危植物邢氏水蕨和海南岛水蕨叶绿体序列rbcl比较及隐种分析

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生态环境学报 2024年第1期33卷 28-34页

作者：董元火蔡方陶白雪依宋呈钰宁婷婷熊飞曾长立江汉大学生命科学学院/湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心武汉湖北430056 湖北省荆楚药材研究院武汉湖北430223

2021年,水蕨属(Ceratopteris)所有植物包括邢氏水蕨(***)、水蕨(***)和粗梗水蕨(***)被列为国家二级重点保护野生植物。水蕨属植物是研究植物性别决定、配子体形态建成以及遗传学、细胞生物学和生物化学等学科的模式植物之一,也是中国... 详细信息

2021年,水蕨属(Ceratopteris)所有植物包括邢氏水蕨(***)、水蕨(***)和粗梗水蕨(***)被列为国家二级重点保护野生植物。水蕨属植物是研究植物性别决定、配子体形态建成以及遗传学、细胞生物学和生物化学等学科的模式植物之一,也是中国重要的野生水生植物种质资源。隐种(Cryptic species)问题已经成为系统分类研究、物种形成机制、生物进化和基于保护目的的种群遗传研究及保护计划共同关注的重要问题。基于叶绿体rbcl序列评价邢氏水蕨和海南岛陵水黎族自治县白水岭(BSL)、陵水黎族自治县木号镇(BJC)、海口城西镇(SPSK)等3个新发现的水蕨种群叶绿体序列rbcl的差异及系统发育关系,分析新发现尚未被评价的3个水蕨种群的隐种类型,为水蕨属不同物种和隐种的保护及利用提供科学依据。结果表明,邢氏水蕨和3个种群水蕨的rbcl序列长度为1227 bp,邢氏水蕨有2个变异位点,分别在783 bp处和1164 bp处。采用邻近法(NJ)构建了水蕨属叶绿体rbcl系统发育树。系统发育树显示BSL、BJC、SPSK等3个种群的水蕨与南方型水蕨隐种聚为同一支,表明这3个地方的水蕨隐种为南方型;而邢氏水蕨单独成一支。遗传距离分析显示BSL、BJC、SPSK种间遗传距离为0,与南方型水蕨之间遗传距离也为0;邢氏水蕨与BSL、BJC、SPSK遗传距离为0.002。结果进一步表明,海南岛的BSL、BJC、SPSK等3个种群水蕨为南方型水蕨,同时为邢氏水蕨的分类提供了佐证。不同的水蕨及隐种应该采取不同的保护策略,且邢氏水蕨应得到优先保护。保护邢氏水蕨和3个南方型水蕨种群的原生境是最有效的保护策略。

关键词：水蕨邢氏水蕨隐种 rbcl 海南岛保护策略

Ulva and Enteromorpha(Ulvaceae,Chlorophyta) from two sides of the Yellow Sea:analysis of nuclear rDNA ITS and plastid rbcl sequence data

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 762-768页

作者：王金锋李楠姜鹏 BOO Sung Min LEE Wook Jae 崔玉琳林瀚智赵瑾刘正一秦松 Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Graduate University of Chinese Academy of Sciences Yantai Institute of Coastal Zone Research for Sustainable Development Chinese Academy of Sciences Department of Biology Chungnam National University Jeju Biodiversity Research Institute College of Resources and Environmental Sciences Nanjing Agricultural University

Ulvacean green seaweeds are common worldwide;they formed massive green tides in the Yellow Sea in recent years,which caused marine ecological problems as well as a social *** investigated two major genera of the Ulvaceae,Ulva and Enteromorpha,and collected the plastid rbcl and nuclear ITS sequences of specimens of the genera in two sides of the Yellow Sea and analyzed *** trees of rbcl data show the occurrence of five species of Enteromorpha(***,***,***,*** and ***) and three species of Ulva(***,*** and ***).However,we found ***,which is known as a subtropical to tropical species,at two sites on Jeju Island,*** ribotypes in partial sequences of 5.8S rDNA and ITS2 from *** were also *** network analysis revealed that the common ribotype,occurring in China,Korea and Europe,is connected with ribotypes from Europe and China/*** samples of the same species were collected from both sides of the Yellow Sea,intraspecific genetic polymorphism of each species was low among samples collected worldwide.

关键词： ITS phylogeography rbcl Ulvaceae warm current Yellow Sea

Phylogenetic analyses of four species of Ulva and Monostroma grevillei using ITS, rbcl and 18S rDNA sequence data

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Chinese Journal of Oceanology and Limnology 2013年第1期31卷 97-105页

作者：林中姮沈颂东陈伟洲李慧慧 Department of Cell Biology School of Biology and Basic Medical SciencesSoochow University Institute of Marine Biology Shantou University

Chlorophyta species are common in the southern and northern coastal areas of China. In recent years, frequent green tide incidents in Chinese coastal waters have raised concerns and attracted the attention of scientists. In this paper, we sequenced the 18S rDNA genes, the internal transcribed spacer （ITS） regions and the rbcl genes in seven organisms and obtained 536-566 bp long ITS sequences, 1 377-I 407 bp long rbcl sequences and 1 718-1 761 bp long partial 18S rDNA sequences. The GC base pair content was highest in the ITS regions and lowest in the rbcl genes. The sequencing results showed that the three Ulvaprolifera （or U. pertusa） gene sequences from Qingdao and Nan＇ao Island were identical. The ITS, 18S rDNA and rbcl genes in U. prolifera and U. pertusa from different sea areas in China were unchanged by geographic distance. *** had the least evolutionary distance from U. californica in both the ITS regions （0.009） and the 18S rDNA （0.002）. These data verified that Ulva and Enteromorpha are not separate genera.

关键词： Ulva ITS region rbcl 18S rDNA phylogeny sequences analysis

Re-identifying Grateloupia yangjiangensis(Rhodophyta, Halymeniaceae) based on morphological observations, life history and rbcl sequence analyses

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Acta Oceanologica Sinica 2014年第4期33卷 77-84页

作者： WANG Hongwei GUO Shaoru ZHANG Xiaoming ZHAO Dan ZHANG Wen LUAN Rixiao College of Life Sciences Liaoning Normal University Dalian Natural History Museum

On the basis of morphological observations, life history and molecular phylogeny, Grateloupia yangjiangensis, which is similar to G. filicina, G. orientalis, G. catenata, and G. ramosissima in appearance, was re-examined. The results are as follows：（1） the auxiliary-cell ampullae of G. yangjiangensis were of Grateloupia type, thalli was fleshy and gelatinous in texture, and the erect axes were compressed; the cortex was 0.25–0.30 mm thick, consisting of five to seven outer layers, and there were five inner layers of triangular or stellate cells;（2） there was no filamentous stage in the development of the carpospores;（3） the ribulose-1,5-bisphosphate carboxylase/oxygenase gene（rbcl） sequence of four G. yangjiangensis examined showed that there was no intergeneric divergence among them, and for the phylogenetic tree, four sequences of G. yangjiangensis formed a single monophyletic subclade within the large Grateloupia clade of Halymeniaceae. In conclusion, G. yangjiangensis was a single species within the genus Grateloupia. This research provided criterion for identification and cultivation of G. yangjiangensis.

关键词： Grateloupia yangjiangensis Halymeniaceae Rhodophyta morphological observations life history rbcl

Cloning and sequence analysis of the partial sequence of the rbcl from Bryopsis hypnoides

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Acta Oceanologica Sinica 2005年第5期24卷 150-161页

作者： TIAN Chao WANG Guangce YE Naihao ZHANG Baoyu FAN Xiaolei ZENG Chengkui Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China

The partial sequence of the rbcl from Bryopsis hypnoides, including the sequences of the upstream, extron and partial intron, was amplified by PCR and their sequences were determined. With Spinacia oleracea as the outgroup, neighbor-joining method and maximum parsimony method were used respectively to build phylogenetic trees according to the rbcl exon sequence among 13 species that were the typical species of six phyla. Two kinds of trees showed clearly that there were two groups among those species, the green lineage and the non-green lineage. And the relationships of algae in the green lineage were similar in the two trees but those in the non-green lineage were not consistent. Analysis of codon preference indicated that the codon preference of the rbcl exon of Bryopsis hypnoides distinctly differed from that of the relevant sequence of photosynthetic bacteria.

关键词： rbcl Bryopsis hypnoides sequence analysis phylogenetic analysis codon preference

Phylogeny and divergence of Chinese Angiopteridaceae based on chloroplast DNA sequence data (rbcl and trnL-F)

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Chinese Science Bulletin 2007年第1期52卷 91-97页

作者： LI ChunXiang LU ShuGang State Key Laboratory of Palaeobiology and Stratigraphy of Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China Insttute of Ecology and Geobotany Yunnan University Kunming 650091 China

Marattioid ferns are an ancient lineage of primitive vascular plants that first appeared in the middle Carboniferous. Extant members are almost exclusively restricted to tropical regions, and the spe- cies-rich family Angiopteridaceae are limited in their distribution to the eastern hemisphere; relation- ships within the group are currently vague. Here the phylogenetic relationship between Angiopteris Hoffm. and Archangiopteris Christ et Gies. was evaluated based on the sequence analysis of chloro- plast rbcl gene and trnL-F intergenic spacer with MEGA2 and MrBayes v3.0b4. On the basis of the phy- logenetic pattern and fossil record, we further estimated the divergence time for the two genera. The phy- logenetic trees revealed that all species of Angiopteris and Archangiopteris in this study formed a monophyletic group with strong statistical support, but the relationship between the two genera re- mained unresolved based on individual sequence analysis. On the other hand, the sequence analyses of combined data set revealed that Archangiopteris species diverged first, indicating that Archangiop- teris may not be a direct derivative as traditionally assumed. The clade of Angiopteris and Archan- giopteris appears to have diversified in the late Oligocene (≈26 Ma) based on the molecular estimate. Thus, the evolutionary history of extant Angiopteris and Archangiopteris has been characterized by ancient origin and recent diversification, and these groups are not relic and endangered lineages as traditionally considered.

关键词： rbcl DNA 中国遗传学

Aian origin for Polystichum (Dryopteridaceae) based on rbcl sequences

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Chinese Science Bulletin 2004年第11期49卷 1146-1150页

作者： LIChunxiang LUShugang YANGQun StateKeyLaboratoryofPalaeobiologyandStratigraphyofNanjingInstituteofGeologyandPalaeontology ChineseAcademyofSciencesNanjing210008China InstituteofEcologyandGeobotany YunnanUniversityKunming650091.China

Chloroplast rbcl sequences of 60 species of Polystichum sensu lato (S.l.), including 23 new sequences from southwest China, were used to assess the phylogenetic relatipmships within the genus. On the basis of estimated evolution rate of rbcl gene and the genetic distance data that passed relative-rate tests, we further estimated the diver- gence times betwcen some clades of the genus. The phyloge- netic relationships were inferred using the neighbor-joining and maximim-parsimony methods, both methods producing trees with completely congruent topology. These trees reveal that all species of Polystichum s.I. in this study (including Cyrtomium and Cyrtomidictyum) form a monophyletic group. The basal split in Polystichum s.I. separates a clade with all Asian members from a elade containing other species from all over the world. The phylogenetic and divergence time estimation results lead us to suggest that Polystichum S.I. originated in Asia in the late Late Cretacous (≈76 Ma) and migrated into other places in the world in early Eocene (≈ 46 Ma).

关键词：亚洲起源系统发育分歧时间 rbcl 基因序列耳蕨

Sequences and Phylogeny Analysis of rbcl Gene in Marine Chlorophyta

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Journal of Ocean University of China 2010年第2期9卷 145-150页

作者： SHEN Songdong LI Yanyan WU Xunjian DING Lanping College of Life Sciences Soochow University Suzhou 215123 P. R. China Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 P. R. China

The rbcl gene of Ulva pertusa,Enteromorpha prolifera and Monostroma grevillei was amplified,sequenced and *** comparing the rbcl sequences with seven other Ulvales species retrieved from GenBank,the sequence divergences and thephyletic evolution were analyzed and the phylogenetic tree was *** the phylogenetic tree,it can be found that ***-tusa,*** and *** group in one branch,while ***,*** and *** cluster in another ***,unlike the Enteomorpha species,the Ulva species do not gather in one *** Ulva and Enteomorpha might beaffiliates of one *** and *** gathered together,which coincided with the morphological ***,the thallus of *** is thick and with many holes,which is different from *** in *** cluster togetherin the phylogenetic tree with a genetic distance of *** results indicate that Ulva and Enteromorpha are not distinguishedstrictly.

关键词： Ulva Enteromorpha Monostroma rbcl phylogeny