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A Bifunctional-Blocker-Aided Hybridization Chain Reaction Lighting-Up Self-calibrating Nanocluster fluorescence for Reliable Nucleic Acid Detection

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Journal of Analysis and Testing 2024年第2期8卷 160-169页

作者： Dan Zhang Guobao Zhou Hongyan Yang Yi Wang Lijun Shen Yuxuan Qiu Lei Li Longhua Guo College of Chemistry and Materials Science Zhejiang Normal UniversityJinhua321004China Jiaxing Key Laboratory of Molecular Recognition and Sensing College of BiologicalChemical Sciences and EngineeringJiaxing UniversityJiaxing314001China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou314000China

In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5′-CAC CGC T-3′and 5′-ATT TGC CTT TTG GGG ACG GATA-3′)at two terminals creates a red-emitting AgNC nucleation sequence(rNS,5′-CAC CGC TAT TTG CCT TTT GGG GAC GGATA-3′).We found that the presence of a toehold fragment(5′-TGCCC-3′)in HP1 could silence the rNS.Upon the addition of a target nucleic acid,HCR of HP1 and hairpin probe 2(HP2)could be initiated,resulting in the formation of long chain of DNA duplexes with multibranched rNS.As the toehold fragment in HP1participated in generating duplexes,a strong emission of rNS-templated AgNCs was observed at 670 nm.More significantly,a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation sequence.On the one hand,the blocker could increase the signal-to-noise-ratio of the constructed biosensor,and on the other hand,the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its reproducibility.Compared with the traditional HCR-AgNCs sensors,the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability,which is important for the fabrication of biosensors in various fields for practical biosensing applications.

关键词： ratiometric fluorescence Hybridization chain reaction Silver nanocluster Biosensor Bifunctional blocker

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Enhanced oxidase-like activity of g-C_(3)N_(4) nanosheets supported Pd nanosheets for ratiometric fluorescence detection of acetylcholinesterase activity and its inhibitor

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Chinese Chemical Letters 2022年第2期33卷 757-761页

作者： Chenghui Zhang Pengjuan Ni Bo Wang Wendong Liu Yuanyuan Jiang Chuanxia Chen Jian Sun Yizhong Lu School of Materials Science and Engineering University of JinanJi’nan 250022China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

The undesirable enzymatic activity of nanozymes under near neutral p H condition and the traditional single signal output always restrict the analytical application of nanozyme-based biosensors.Herein,graphitic carbon nitride nanosheets supported palladium nanosheets composite (Pd/g-C_(3)N_(4)) with both oxidase-like activity and fluorescent property is synthesized.Notably,Pd/g-C_(3)N_(4)exhibits enhanced oxidase-like activity compared to Pd NSs under p H 7.4.By combining Pd/g-C_(3)N_(4)with o-phenylenediamine(OPD),a ratiometric fluorescence assay for acetylcholinesterase (ACh E) activity detection is developed.Pd/g-C_(3)N_(4)can catalyze oxidation of nonfluorescent OPD to fluorescent oxidized OPD (ox OPD,Em=565nm),which can quench fluorescence of g-C_(3)N_(4)supporter (Em=441 nm) through fluorescence resonance energy transfer (FRET).However,in presence of ACh E,acetylthiocholine can be hydrolyzed into thiocholine,which will block the oxidase-like activity of Pd/g-C_(3)N_(4)and then hamper the FRET process.This ratiometric fluorescence assay is also viable to screen ACh E inhibitor.This work will guide design of ratiometric fluorescence assay based on nanozymes with improved enzymatic activity.

关键词： Oxidase-like activity ratiometric fluorescence Acetylcholinesterase g-C_(3)N_(4)nanosheets Pd nanosheets

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Field-portable ratiometric fluorescence imaging of dual-color label-free carbon dots for uranyl ions detection with cellphone-based optical platform

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Chinese Chemical Letters 2020年第11期31卷 2925-2928页

作者： Jingjing Qian Nana Cao Jian Zhang Jinjin Hou Qian Chen Cheng Zhang Yudie Sun Shengjun Liu Lifang He Kui Zhang Haibo Zhou Department of Applied Chemistry Anhui Agricultural of UniversityHefei 230036China School of Chemistry and Chemical Engineering Anhui University of TechnologyMa'anshan 243032China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou 510632China Second Clinical Medical College(Shenzhen People's Hospital) Jinan UniversityShenzhen 518020China

Under the public spotlight,uranyl(UO22+)ions has attracted considerable attention for the extreme radioactive and chemical toxicity to ourselves and our environment.Herein,we present a simple and effective ratiometric fluorescence imaging method for the visualizing and quantitative detection UO22+ions by cellphone-based optical platform.The sensing solution was prepared by mixing label-free red carbon dots(r-CDs)and blue carbon dots(b-CDs)together with a fixed photoluminescence intensity ratio of 4:1.When UO22+ions were added,the fluorescence of r-CDs can be selectively quenched,while the fluorescence of b-CDs remains stable without spectral cha nges.With the gradually increase the amounts of UO22+ions,the different response of dual-color CDs resulted in a signification color evolution from deep red to dark purple under the ultraviolet(UV)light illumination.Then,a cellphone-based optical platform was constructed for directly imaging the color change of the samples,and the built-in Colorpicker APP quickly output the red,green and blue(RGB)channel values of these images within one second.Interesting,there was a linear relationship between the ratio of red and blue(R/B)channel values and UO22+ions concentration from 0μmol/L to 30.0μmol/L(R^2=0.92804)with the detection limit of^8.15μmol/L(signal-to-noise ratio of 3).In addition,the optical platform has also been applied to the quantification of UO22+ions in tap water and river water sample.With the advantage of low-cost,portable,easy to operation,we anticipate that this method would greatly improve the accessibility of UO22+ions detection even in resource-limited areas.

关键词： Uranyl ions Carbon dots ratiometric fluorescence Field-portable Cellphone

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Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy

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Nano Research 2022年第7期15卷 6515-6521页

作者： Xin Zhang Juan Chen Jiwen Hu Anna du Rietz Xiongyu Wu Ruilong Zhang Zhongping Zhang Kajsa Uvdal Zhangjun Hu Department of Physics Chemistryand Biology(IFM)Linköping UniversityLinköping SE 58183Sweden School of Chemistry and Chemical Engineering Anhui UniversityHefei 230601China

Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria,thereby pH fluctuation is one of the most significant indicators for tracking mitophagy.Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features,including mitochondrial-specificity,excellent biocompatibility,wide pH-sensitive range of 8.0–4.0,and especially quantitative ability.However,available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule.To fully address this issue,we herein integrate two fluorogenic pH sensitive units,a mitochondria-specific block,cellpenetrating facilitator,and biocompatible segments into an elegant silica nano scaffold,which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy.Most significantly,at a single wavelength excitation,the integrated pHsensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH,thus enabling a ratiometric analysis of pH variations during the whole mitophagy.This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.

关键词： mitochondrial pH mitophagy ratiometric fluorescence nanoprobe cell imaging

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Fluorescent MOF-based nanozymes for discrimination of phenylenediamine isomers and ratiometric sensing of o-phenylenediamine

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Chinese Chemical Letters 2022年第4期33卷 2081-2085页

作者： Yinghui Xia Kunming Sun Ya-Nan Zuo Shuyun Zhu Xian-En Zhao School of Chemistry and Chemical Engineering Qufu Normal UniversityQufu 273165China

Although peroxidase-like nanozymes have made great progress in bioanalysis,few current nanozymebased biosensors are constructed for discriminating isomers of organic compounds.Herein,fluorescent metal-organic framework(MOF)-based nanozyme is utilized for phenylenediamine isomers discrimination and detection.NH_(2)-MIL-101(Fe),as a member of Fe-based MOFs,functions as not only fluorescent indicator but also peroxidase mimics.In the presence of H_(2)O_(2),NH2-MIL-101(Fe) can catalyze the oxidation of o-phenylenediamine(OPD) and p-phenylenediamine(PPD) into their corresponding oxidation products(OPDox and PPDox),which in turn quench its intrinsic fluorescence at 445 nm via inner filter effect(IFE).Differently,a new fluorescence peak at 574 nm is observed for OPDox.Thus,a ratiometric fluorescence method for the detection of OPD can be designed with the fluorescence intensity ratio F_(574)/F_(445)as readout.This proposed strategy displays excellent discrimination ability for three phenylenediamines and may open new applications of MOFs in environmental science.

关键词： Bifunctional MOF Nanozyme Photoluminescence Aromatic diamine isomers ratiometric fluorescence

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Eu^(3+)-doped Bovine Serum Albumin-derived Carbon Dots for ratiometric Fluorescent Detection of Tetracycline

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Journal of Analysis and Testing 2022年第4期6卷 365-373页

作者： Tian-Tian Zhang Zi-Han Chen Guo-Yue Shi Min Zhang School of Chemistry and Molecular Engineering Shanghai Key Lab for Urban Ecological Processes and Eco-RestorationEast China Normal UniversityShanghai200241China

The abuse of antibiotics has become a global concern,thus it is significant to develop simple antibiotic assays.Herein,a novel type of lanthanide-doped bovine serum albumin(BSA)derived carbon dots(BCDs)dubbed Eu-BCDs has been unveiled for ratiometric fluorescent detection of tetracycline(TC),which was formed from the one-step hydrothermal reaction of Eu^(3+)and BSA.Upon challenge with TC,the blue fluorescence of Eu-BCDs at 448 nm was gradually quenched due to the internal filtration effect,while the characteristic red fluorescence of Eu^(3+)at 618 nm was significantly enhanced owning to the antenna effect,leading to the ratiometric fluorescent detection of TC with fast response(2 min),a wide linear dynamic range(0-80μmol/L),and high selectivity and sensitivity(limit of detection(LOD)of 3 nmol/L).Moreover,Eu-BCDs can be successfully applied for the ratiometric fluorescent detection of TC in environmental and biological samples,such as river water,milk,honey and serum.The Eu-BCDs sensing platform would have great application potential in view of its simple preparation,rapid response,high sensitivity and good selectivity.

关键词： carbon dots Europium ion ratiometric fluorescence Tetracycline

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A Novel ratiometric Fluorescent Probe for Cyanide Anion with High Selectivity and Its Application in Cell Imaging

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Chemical Research in Chinese Universities 2017年第4期33卷 534-539页

作者： FENG Yusha BAI Lifei WANG Shaodan KONG Xianyi CONG Lele ZHAO Qing YANG Qingbiao LIYaoxian Department of Neurology China-Japan Union Hospital of Jilin University Changehun 130031 P. R. ChinaI College of Chemistry Jilin University Changchun 130021 China Jiangsu Key Laboratory of Biofunction Molecule College of Life Science and Cheimistry & Chemical Engineering Jiangsu Second Normal University Nanjing 210013 P. R. China

A novel hybrid acenaphthene-hemicyanine dye（Acc） was designed and synthesized as a colorimetric and ratiometric fluorescent sensor for cyanide detection. The nucleophilic addition of cyanide anion resulted in the blocking of the intramolecular charge transfer（ICT）. Thus, the sensor exhibited high sensitivity and selectivity to- wards cyanide anion among many anions in dimethylsulphoxide（DMSO）-water media, with the emission wavelength displaying a very large shift（186 nm）. Moreover, the color changes of the solution could be observed by the naked eyes and it had a short response time. Under optimized conditions, the detection limit for cyanide was calculated to be 1.3μmol/L（R2=0.9958）. In addition, the probe had excellent biocompatibility and could be utilized to monitor cyanide in living cells.

关键词： ratiometric fluorescence Cyanide Nucleophilic addition Bioimaging

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A Novel ratiometric Fluorescent Probe with Selective Recognition for Sulfite in Aqueous Solution

A Novel Ratiometric Fluorescent Probe with Selective Recogni...

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全国第十四届大环化学暨第六届超分子化学学术讨论会

作者： SUN Yi-min ZHONG Cheng FU En-qin~* Department of Chemistry,Wuhan University,Wuhan(430072)P.R.China

A new fluorescence resonance energy transfer(FRET)based ratiometric fluorescent chemosensor for sulfite has been synthesized.Receptor 1 exhibits a unique selectivity for SOover other anions(F,Cl,Br,I,AcO,SO,HCO,HPO,HP... 详细信息

关键词： sulfite anion recognition ratiometric fluorescence

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A highly selective colorimetric and turn-on fluorescent probe for cyanide anion

A highly selective colorimetric and turn-on fluorescent prob...

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全国第十六届大环化学暨第八届超分子化学学术讨论会

作者： Hongda Li Bingzhu Yin Key Laboratory of Natural Resources of Changbai Mountain ＆ Functional Molecules（Yanbian University）Ministry of Education

＜正＞Cyanide is extremely toxic to mammals with even a small amount of this species,leading to vomiting,loss of consciousness,and eventually to death.Therefore,its concentration in drinking water is limited to less than 2 uM according to the World Health Organization.So we synthesized a simple yet effective probe 1 and 2 for cyanide ion in the aqueous phase.

关键词： coumarin cyanide recognition ratiometric fluorescence

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