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Identification of random amplified polymorphic dna Markers Linked to Sex Determination in Calamus simplicifolius C. F. Wci

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Journal of Integrative Plant Biology 2005年第10期47卷 1249-1253页

作者： Hua YANG Si-Ming GAN Guang-Tian YIN Huang-Can XU Research Institute of Tropical Forestry Chinese Academy of Forestry Longdong Guangzhou 510520 China Zhejiang Academy of Forestry Hangzhou 310023 China

The random amplified polymorphic dna （RAPD） molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, dna samples were extracted individually from 10 male and 10 female plants. After a total of 1 040 decamer primers had been tested, an approximate 500-bp male-specific dna fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.

关键词： Calamus simplicifolius random amplified polymorphic dna rattan sex determination.

Use of random amplified polymorphic dna （RAPD） analysis to detect jujube witches＇ broom phytoplasmas

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Journal of Agricultural Science and Technology 2009年第4期3卷 1-4,16页

作者： FAN Xin-ping TIAN Jian-bao Bertaccini Assunta Pomology Institute Shanxi Academy of Agricultural Science Taigu 030815 China DiSTA Patologia Vegetale Alma Mater Studiorum Universitgt di Bologna Bologna 40127 Italy

Jujube witches＇ broom is a devastating disease of Ziziphusjujube that occurs in various jujube regions of China. Nucleic acid extracted from midribs of samples collected from three jujube varieties （＂Suanzao＂, ＂Lajiaozao＂ and ＂Langzao＂） from symptomatic and asymptomatic shoots were tested by random amplified polymorphic dna analyses. Using 13 different 10 and 11-bp random primers the amplification of jujube dna was achieved from all the samples; AMI4 primer provided amplification of specific dna fragments of about 400 bp, only from samples collected from symptomatic plants. No genetic variations in these varieties were identified using the other 11 arbitrary primers; only with primer AL07 it was possible to differentiate ＂Langzao＂ from the other two varieties tested. All the experiments were repeated 2 times and the results were consistent. Compared with PCR analyses with phytoplasma-specific primers, RAPD techniques resulted to be an alternative rapid and sensitive method for detecting jujube phytoplasmas presence in different jujube varieties.

关键词： jujube witches' broom phytoplasmas random amplified polymorphic dna detection

Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions

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Journal of Forestry Research 2011年第1期22卷 123-126页

作者： LIU Zong-yue NING Fang-yong YANG Hong-yan ~ WEI Lai BAI Xiu-juan Animal science and technology Institute of Northeast Agriculture University Harbin China.

Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis （BSA） in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into sequence-characterized amplified regions （SCAR） marker for rapid detection of this disease. A single RAPD marker A8 amplified a specific band of 263bp in self-biting minks, which was designated as SRA8-250, and non-specific band of 315bp in both self-biting and healthy minks. The sequences of the bands exhibited 75% and 88% similarity to Canis familiarizes major histocompatibility complex （MHC） class II region and Macaca mulatta MHC class I region, respectively. A SCAR marker SCAR-A8 was designed for the specific fragment SRA8-250 and validated in 30 self-biting minks and 30 healthy minks. Positive amplification of SCAR-A8 was detected in 24 self-biting minks and 12 healthy minks. χ2 test showed significant difference （p〈0.01） in the detection rate between the two groups. This indicated that SRA8-250 can be used as a positive marker to detect self-biting disease in minks. Furthermore, the finding that self-biting disease links with MHC genes has significant implications for the mechanism of the disease.

关键词： mink random amplified polymorphic dna self-biting sequence characterized amplified region

Study on the Mediterranean coastal seaweed Ulva linza exposed to natural and stressed environmental conditions

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Journal of Coastal Life Medicine 2015年第10期 766-775页

作者： Ayman Abd Elmoneim El-Gamal Eman Mohamed Fakhry Dahlia Mohamed El-Maghraby Department of Marine Geology Coastal ResearchInstituteNational Water Research Center15El-Pharanna StreetEl-Shallalat21514 AlexandriaEgypt Department of Botany and Microbiology Faculty of ScienceAlexandria University21511 AlexandriaEgypt

Objective:To study Ulva linza(***)as macro-alga exposed to different levels of ionizing radiation from the natural background of ***:Samples of *** were collected from two different habitats at the two terminals of the rocky shore of Abu Qir Bay,Alexandria,*** western terminal at Abu Qir represented an area of normal background radiation while the eastern terminal at Rosetta represented an area of relatively high background *** between the algae grown in areas of relatively high and normal background radiation was investigated by using different ***,the ambient water quality was measured and the concentrations of natural radionuclides(^(238)U,^(232)Th and^(40)K)bio-accumulated in the tested macro-alga were ***:The algae naturally exposed to radionuclides(^(238)U series,^(232)Th series and^(40)K)were *** dose rates in *** inhabiting in Rosetta and Abu Qir were calculated as 70.12 and 42.67 nGy/h,*** analysis of algal samples demonstrated that total pigment contents were 2.21 and 2.19 mg/g on a fresh weight basis for *** inhabiting in Rosetta and Abu Qir,*** acid compositions showed comparable profiles for both algal samples with saturated fatty acids as major *** results of protein profiles confirmed slight differential expression in protein ***-related randomly amplified polymorphic dna provided evidence that both samples were strongly *** using transmission electron microscopy,no obvious ultra structural changes in the examined cells were ***:These experimental results demonstrate that radiation doses are not high enough to cause damage or manifest any significant variation in Ulva tissues.

关键词： Ulva linza Radionuclides Protein profile random amplified polymorphic dna Electron microscopy

RAPD Markers and Genetic Information Entropy in Environmental Monitoring: A Case Study with Wild Mushrooms

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Journal of Geoscience and Environment Protection 2023年第9期11卷 28-39页

作者： Charoula Psallida Dimitrios Argyropoulos Genetic Identification Laboratory Institute of Technology of Agricultural Products Hellenic Agricultural Organization-DIMITRA Athens Greece

Mushrooms have a remarkable scientific value due to their nutritional, medicinal properties and industrial applications in enzyme production, so that effort in the maintenance of native wild mushroom varieties is increasing. The present study focuses on the use of random amplified polymorphic dna (RAPD) markers for biodiversity measure of wild mushroom species of the Northwest mountainous region of Greece. Data mining of similarity matrices from RAPD analysis was used to extract measurable entropy parameters for mushroom biodiversity monitoring based on Shannon’s information entropy. Shannon information index provides an easy assessment of the entropy of the genetic information of the germplasm per mushroom species while the total equitability index (EH) = 0.871 offers an overall estimation of the genetic variation evenness of all species in the population of the studied mushrooms. Application of RAPDs with parallel entropy analysis is an easily applicable and low-cost valuable technology in environmental monitoring, using genetic information of wild mushroom species as an indicator that can lead to future actions in biodiversity maintenance and germplasm protection. The provided methodology can serve as a pilot procedure enriched with other environmental factors to monitor and protect wild mushroom communities native to the Greek countryside or in any part of the world and provide comparable results about biodiversity from different regions using common entropy indices.

关键词： random amplified polymorphic dna Shannon’s Index Entropy Biodiversity Dendrogram