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Transplantation of bone marrow stromal cells overexpressing humanvascular endothelial growth factor 165 enhances tissue repair in a ratmodel of radiation-induced injury

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Chinese Medical Journal 2014年第6期127卷 1093-1099页

作者： Wang Tao Liao Tian'an Wang Hong Deng Wei Yu Dahai Department of Oral and Maxillofacial Surgery People's Hospital of Hainan Province Haikou Hainan 570311 China Department of Oral Maxillofacial Surgery Stomatological HospitalGuangxi Medical University Nanning Guangxi 530021 China

Background The multilineage differentiation potential ability of bone marrow stromal cells(BMSCs) showed great potential in tissue engineering, while vascular endothelial growth factor 165(VEGF165) promotes vasculogenesis and further promotes tissue regeneration. This study aimed to assess the ability of rat BMSCs expressing human VEGF A165(hVEGF165) to promote tissue repair in rat model of radiation-induced injury.Methods Rat BMSCs were isolated from the tibia. Plasmid DNA expressing hVEGF165 was stably transfected into BMSCs using liposomes. The right hindlimb muscle of 40 rats was irradiated using a 60 Co γ source(total dose 30 Gy). The animals were divided into four groups(n=10): not injected with BMSCs(control; group 1) or intramuscularly injected two times(once in 2 weeks) with pcDNATM3.1-transfected BMSCs(group 2), untransfected BMSCs(group 3), or hVEGF165-transfected BMSCs(group 4). Angiography was performed 1 week after the last injection of BMSCs; samples of the hindlimb muscle were subjected to transmission electron microscopy, ultrastructural analysis, reverse transcription-PCR(RT-PCR), Western blotting, and immunohistochemistry.Results Rat BMSCs with multipotent differentiation capacity were isolated. hVEGF165-transfected BMSCs overexpressed hVEGF165 mRNA and protein. Injection of BMSCs(groups 2–4) increased the average vessel number, density, diameter, and cross-sectional area; mRNA expression of the myogenic markers including myoblast determination protein, myogenin, and α-smooth muscle actin; and CD31 protein expression; and promoted the repair of blood vessels and myofibers after radiation-induced injury compared to group 1; each of these parameters and hVEGF165 mRNA or protein expression were markedly improved in rats injected with hVEGF165-transfected BMSCs compared to groups 2 and 3.Conclusions BMSCs expressing hVEGF165 enhanced the repair of radiation-induced tissue injury by promoting vasculogenesis and muscle fiber regeneration. BMSCs expressing hVEGF165 may have potential clinical applicat

关键词： bone marrow stromal cell vascular endothelial growth factor radiation-induced tissue injury

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Rabbit model of radiation-induced lung injury

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Asian Pacific Journal of Tropical Medicine 2013年第3期6卷 237-241页

作者： Zhen-Zong Du Hua Ren Jian-Fei Song Li-Fei Zhang Feng Lin Hai-Yong Wang Cardio-Thoracic Surgery Affiliated Hospital of Guilin Medical University Cardio-Thoracic Surgery Peking Union Medical College Hospital

Objective:To explore the feasibility of establishing an animal model of chronic radiationinduced lung injury.Methods:Twenty-eight New Zealand white rabbits were randomly divided into 3 groups(the right lung irradiation group,the whole lung irradiation group and the control group).Animal model of radiation-induced lung injury was established b) highdoes radiotherapy in the irradiation groups,then all rabbits underwent CT and pathological examinations at 1.***.4.8.12.16 weeks,respectively after radiation.Results:Within 4 weeks of irradiation,some rabbits in the right lung irradiation group and whole lung irradiation group died. CT and pathological examinations all showed acute radiation pneumonitis.At 8-12 weeks after irradiation,CT scanning showed ground glass samples signs,patchy shadows and fibrotic stripes. Pathological examination showed the fibrosis pulmonary alveolar wall thickened obviously. Conclusions:The clinical animal model of chronic radiation-induced lung injury which corresponds to practical conditions in clinic can be successfully established.

关键词： New Zealand rabbit Chronic radiation-induced injury Model

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Correlation between miR-564, TGF-β1, and radiation-induced lung injury

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Oncology and Translational Medicine 2019年第5期5卷 204-210页

作者： Yunzhang Ge Tao Xie Bin Yang Qianxia Li Qingrong Ren Xiaoyi Zhou Desheng Hu Zhongshu Tu Hubei Province Tongcheng County Hospital of Traditional Chinese Medicine Xianning 437400China Hubei Cancer Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyHuBei Key Laboratory of Medical Information Analysis and Tumor Diagnosis&TreatmentWuhan 430079China Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective Our study aimed to analyze the expression of miR-564 and TGF-β1 in cancer tissues and the serum of patients with radiation-induced lung injury,and to investigate the relationship between them and radiation-induced lung injury.Methods In situ hybridization and real-time fluorescence quantitative method were used to detect the expression of miR-564.Additionally,immunohistochemistry and enzyme-linked immunosorbent assay(ELISA)were performed to detect the expression of TGF-β1.Results The overall incidence of acute radiation pneumonia was 55.9%(100/179).The incidence of≥grade 2 radioactive pneumonia was 24.0%(43/179)and that of grade 1 was 31.8%(57/179).The expression of miR-564 in grade≥2 was slightly higher than that in patients without or with grade 1,but there was no statistical difference(P=0.86).The serum level and ratio of miR-564 in patients with grade≥2 were significantly higher than those without or with grade 1(P=0.005,P=0.025,respectively).The expression of TGF-β1 in grade≥2 was significantly higher than that of patients without or with grade 1(P=0.017).The serum levels of TGF-β1 in grade≥2 were significantly higher than those in patients without or with grade 1(P=0.038).Although the ratio of TGF-β1 in radiation pneumonia of grade≥2 was significantly higher than that of without or with grade 1,there was no significant difference(P=0.24).Moreover,patients with higher expression of miR-564 and lower expression of TGF-β1 had better prognosis.Conclusion MiR-564 and TGF-β1 are predictors of radiation-induced lung injury.Monitoring its changing trend can improve the accuracy of predicting radiation-induced lung injury.The levels and ratio of serum miR-564 and TGF-β1 in patients with radiation-induced lung injury are related to the severity of radiationinduced lung injury.

关键词： radiation-induced lung injury miR-564 TGF-β1

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Adsorbent for Arsenic(V) Removal Synthesized by radiation-induced Graft Polymerization onto Nonwoven Cotton Fabric

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International Journal of Organic Chemistry 2012年第3期2卷 173-177页

作者： Hiroyuki Hoshina Makikatsu Takahashi Noboru Kasai Noriaki Seko Environment and Industrial Materials Research Division Quantum Beam Science Directorate Environment and Industrial Materials Research Division Quantum Beam Science Directorate Japan Atomic Energy Agency Takasaki Japan ERH Techno Research Co. Ltd. Takasaki Japan

A fibrous adsorbent for arsenic (As) removal was synthesized with nonwoven cotton fabric as a trunk polymer. 2-hydroxyethyl methacrylate phosphoric acid monomer which composed of phosphoric acid mono (50%) and di (50%) ethyl methacrylate ester was introduced with radiation-induced graft polymerization onto nonwoven cotton fabric. The degree of grafting of 130% was obtained at irradiation dose of 20 kGy with 5% of monomer solution for 2 hours reaction time at 40?C reaction temperature. After the grafted material was contacted with 10 mmol/L of zirconium (Zr) solution at pH 1, 0.38 mmol/g of Zr was loaded on phosphoric units as a functional group for As(V) removal. The resulting adsorbent was evaluated by column mode adsorption with 1 mg/L of As(V) solution at various pH with space velocity 200 h–1. The maximum capacity of As(V) adsorption was 0.1 mmol/g at pH 2.

关键词： Arsenic Removal Cotton radiation-induced Graft Polymerization Phosphoric Acid Zirconium

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Rapid Biodiesel Fuel Production Using Novel Fibrous Catalyst Synthesized by radiation-induced Graft Polymerization

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International Journal of Organic Chemistry 2011年第2期1卷 20-25页

作者： Yuji Ueki Nor Hasimah Mohamed Noriaki Seko Masao Tamada 不详

An efficient fibrous catalyst for the biodiesel fuel production has been synthesized by radiation-induced graft polymerization of 4-chloromethylstyrene onto a nonwoven polyethylene (NWPE) fabric followed by amination with trimethylamine (TMA) and further treatment with NaOH. The degree of grafting of NWPE fabric and TMA group density of fibrous catalyst could easily and reproducibly be controlled within a range of up to 340% and 3.6 mmol-TMA/g-catalyst, respectively. In the transesterification of triglycerides and ethanol using the synthesized fibrous catalyst, the conversion ratio of triglycerides reached 95% after 4 h reaction at 50°C.

关键词： Biomass Biodiesel Fuel Triglycerides Heterogeneous Catalysis radiation-induced Graft Polymerization Renewable Resources

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Factors Predicting the Relapse of radiation-induced Organizing Pneumonia after Breast-Conserving Therapy

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Open Journal of Radiology 2015年第3期5卷 159-169页

作者： Yukinori Okada Shingo Sakamoto Tatsuyuki Abe Mio Shinozaki Hiromichi Gomi Yoshihide Kanemaki Shin Matsuoka Yasuo Nakajima Department of Radiology St. Marianna University School of Medicine Kawasaki Japan Department of Radiology Breast and Imaging Center St. Marianna University School of Medicine Kawasaki Japan

We investigated the factors predicting radiation-induced organizing pneumonia (RIOP) relapse after tangential breast irradiation. The participants included 23 patients diagnosed with RIOP at the St. Marianna University School of Medicine Hospital between January 2008 and March 2015. Relapse was defined as the appearance of new lesions on diagnostic images during follow-up or after commencing treatment. The relapse-free survival rate and the following 9 parameters were compared between patients with and without RIOP relapse: 1) age (less than vs. equal to or more than the median);2) white blood cell count (less than vs. equal to or more than the median);3) C-reactive protein (CRP) level at the time of RIOP diagnosis (less than normal, more than normal/ less than borderline, and more than borderline);4) boost irradiation (yes vs. no);5) maximum lung depth on linacgraphy (less than vs. equal to or more than the median);6) hormone therapy (yes vs. no);7) chemotherapy (yes vs. no);8) RIOP ratio in the whole lung (less than vs. equal to or more than the median) at the time of RIOP diagnosis;and 9) use of corticosteroids (yes vs. no). The Kaplan-Meier method was used for statistical analysis, with relapse as the cutoff. The follow-up period spanned the date of RIOP onset to May 30, 2015. The level of significance for 2-sided tests was p radiation-induced organizing pneumonia (RIOP) relapse after tangential breast irradiation. The participants included 23 patients diagnosed with RIOP at the St. Marianna University School of Medicine Hospital betwee

关键词： radiation-induced Organizing Pneumonia Breast-Conserving Therapy Tangential Irradiation C-Reactive Protein

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Metastatic peritoneal sarcomatosis from radiation-induced osteosarcoma in a patient with previously treated pelvic Ewing’s sarcoma: Report of a case

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Open Journal of Clinical Diagnostics 2013年第2期3卷 52-56页

作者： Rajendra Kumar Romil Y. Patel John E. Madewell Tariq J. Al-Zaid Jeanne M. Meis Department of Diagnostic Imaging The University of Texas MD Anderson Cancer Center Houston USA Department of Pathology The University of Texas MD Anderson Cancer Center Houston USA

Metastatic peritoneal sarcomatosis most commonly occurs from primary soft tissue sarcomas arising either within the abdomen or extremities. Metastatic peritoneal sarcomatosis from an osteosarcoma is extremely rare;only six cases have previously been reported. We report the first case of metastatic peritoneal sarcomatosis originating from radiation-induced osteoblastic osteosarcoma in a 22-year-old woman who had previously been treated for pelvic Ewing’s sarcoma. Abdominal computed tomography, bone scintigraphy and FDG PET/CT demonstrated extensive finely nodular disseminated peritoneal lesions. Histopathologic examination of these peritoneal lesions revealed osteosarcomatosis. In summary, we describe an unusual case of metastatic peritoneal sar comatosis from secondary osteosarcoma arising in a previously irradiated pelvicEwing’s sarcoma.

关键词： Peritoneal Sarcomatosis Peritoneal Osteosarcomatosis radiation-induced Osteosarcoma radiation-induced Bone Sarcoma

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Prevention and treatment of radiation-induced lung injury by hydroxypiperquin phosphate: a clinical and experimental study

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Journal of Medical Colleges of PLA(China) 2000年第1期15卷 76-78页

作者：闵碧荷王梅肖作平孟沛霖蓝莉

Objective: To evaluate the hydroxypiperquin phosphate (HPQP) as a modifier of radiation-induced injury in human and rat lungs. Methods: Sixty-five patients with lung cancer treated with conventional radiotherapy were divided into 2 groups randomly: Thirty cases were treated with HPQP and the others were in a control group. The changes of X - ray manifestation before, after and during taking drug were compared. An animal model of radiation-induced fibrosis of lungs was also established. Hydroxyproling (HP) content in lung tissue and the pathological changes in rat lungs were checked with microscope and electron microscope after 4 months and 6 months respectively. Results: The changes of lung X-ray manifestation in treatment group were much lighter than that in control group. The HP content and the change of pathology in the lungs of those rats with HPQP treatment were obviously less than that in control group. Conclusion: HPQP plays an important role in prevention and treatment of radiation-induced injury in lungs.

关键词： hydroxypiperquin phosphate radiation-induced lung injury

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radiation-induced brain injury after a conventional dose of intensity-modulated radiotherapy for nasopharyngeal carcinoma:a case report and literature review

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Oncology and Translational Medicine 2020年第1期6卷 30-35页

作者： Qian Zhang Jie Tang Jiayu Du Xiaojie Ma Department of Oncology affiliated Hospital of North Sichuan Medical CollegeNanchong 637000China Medical Imaging College North Sichuan Medical CollegeNanchong 637000China Department of Clinical Medicine North Sichuan Medical CollegeNanchong 637000China

A 61-year-old female nasopharyngeal carcinoma patient was admitted to the hospital with sudden cognitive dysfunction one month after Volumetric Intensity Modulated Arc Therapy(VMAT)conventional dose radiotherapy,and the initial diagnosis was radiation-induced brain injury(RBI).After comprehensive treatment with steroid hormones,the patient’s condition rapidly improved.Typically,in nasopharyngeal carcinoma patients treated with VMAT,the incidence of RBI is extremely low when the temporal lobe dose is less than 65 Gy or 1%of the volume is less than 65 Gy.When this limit is exceeded,RBI may occur in varying degrees.However,in this case,even though the temporal lobe dose was under the prescribed limit,the patient still experienced RBI.The rare observations in this case can be used as a reference,and clinicians should seriously consider the possibility of RBI in similar cases.

关键词： radiation-induced brain injury nasopharyngeal carcinoma VMAT

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Effect of Tirapazamine and Mild Temperature Hyperthermia on the Recovery from radiation-induced Damage in Pimonidazole-Unlabeled Quiescent Tumor Cell Population

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Journal of Cancer Therapy 2013年第2期4卷 521-528页

作者： Shin-Ichiro Masunaga Yoshinori Sakurai Hiroki Tanaka Minoru Suzuki Natsuko Kondo Masaru Narabayashi Keizo Tano Akira Maruhashi Koji Ono radiation Life and Medical Science Research Division Research Reactor Institute Kyoto University Kyoto Japan radiation Life and Medical Science Research Division Research Reactor Institute Kyoto University Kyoto Japan.

The aim in this study is to examine the effect of tirapazamine (TPZ) and mild temperature hyperthermia (MTH) on the repair of radiation-induced damage in pimonidazole-unlabeled quiescent (Q) tumor cells. Labeling of proliferating (P) cells in C57BL/6J mice bearing EL4 tumors was achieved by continuous administration of 5-bromo-2-deoxyuridine (BrdU). Tumors were irradiated with γ-rays at 1 h after the administration of pimonidazole followed by TPZ treatment or MTH. Twenty-four hours later, assessment of the responses of Q and total (= P + Q) cells were based on the frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of the pimonidazole-unlabeled tumor cell fractions was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. With γ-rays only, the pimonidazole-unlabeled cell fraction showed significantly enhanced radio-sensitivity compared with the whole cell fraction more remarkably in Q cells than total cells. However, a significantly greater decrease in radio-sensitivity in the pimonidazole-unlabeled than the whole cell fraction, evaluated using a delayed assay, was more clearly observed in Q cells than total cells. Post-irradiation MTH more remarkably repressed the decrease in radio-sensitivity in the Q cell than the total cells. Post-irradiation TPZ administration produced a large radio-sensitizing effect on both total and Q cells, especially on Q cells. On the other hand, in pimonidazole-unlabeled cell fractions in both total and Q cells, TPZ suppressed the reduction in sensitivity due to delayed assay much more efficiently than MTH, whereas no radio-sensitizing effect was produced. Not only through suppressing the recovery from radiation-induced damage but also through radio-sensitizing effect, post-irradiation TPZ administration is very useful for repressing the increase in the difference in radio-sensitivity due to the delayed assay not only between total and Q tumor cells but also between the pimonidazole-unlabeled and

关键词： Quiescent Cell Recovery from radiation-induced Damage Tirapazamine Pimonidazole Mild Temperature Hyperthermia

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