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第十三届全国量子化学会议

作者： Xiaoyong Zhang Lung Wa Chung Department of Chemistry South University of Science and Technology of China

Recently, a lactate racemase was discovered as a new Ni-dependent enzyme with a unique tethered NAD-like cofactor. We report the computational study aimed at decipher ing the previously unclear role of the Ni-tethered cofactor in reactions of lactate racemase. Our results suggest that the formation of the metastable and reactive NADH-like pyruvate intermediate should avoid a thermodynamic sink by forming a stable pyruvate product and instead promoting its further transformation to produce stable lactate isomers. This result provides new insight into the design of a reactive metal-tethered NADH-like complex for synthetic hydrogenation.

关键词： Density functional calculations Enz yme catal ysis Metalloenz ymes Nickel racemase

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Activity-dependent sulfhydration of serine racemase controls synaptic plasticity in the hippocampus via increasing D-serine availability

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中国药理学通报 2015年第B11期31卷 51-51页

作者： LIYuan-long WuPeng-fei LONGLi-hong HUZhuang-li WANGFang CHENJian-guo DeptofPharmacology TongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhanHubei430030

Aims More and more advances show that hydrogen sulfide （H2S） sulfhydrates functional proteins and regulates their actions. However, much less is known about the endogenous sulfhydration of key proteins under physiological conditions. Methods Biotin-switch assay, electrophysiological recording, RNA interference, full ESI-MS scan et al. Results Herein, we found that the protein sulfhydration was dynamically regulated by neuro- nal activity. H2S sulfhydrated and activated serine racemase （SR） in the hippocampus, resuhantly increased D- serine level. Notably, sulfhydration of SR was markedly promoted by neuronal stimulation and underlay activity-de- pendent changes of D-serine availability. Both genetic knockdown and pharmacological inhibition of cystathionine β-synthase （CBS）, the key H2S-producing enzyme in brain, attenuated hippoeampal long-term potentiation （LTP） , which can be reversed by exogenous supplement of H2S or D-serine. We also observed that the increase in LTP induced by H2S is dependent on D-serine and polysulfides. In aged rats, sulfhydration of SR was significantly decreased. Furthermore, exogenous supplement of H2S restored the level of sulfhydration and reversed the age-re- lated deficits in hippocampal LTP. Conclusion Our data provide direct evidence for the biological significance of endogenous sulfhydration in physiological conditions and demonstrate a pivotal role of activity-dependent sulfhydra- tion in synaptic plasticity.

关键词： hydrogen sulfide (H2S) polysulfides sulfhydration D-serine serine racemase (SR) synapticplasticity

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A variety of markers combined use of clinical significance for diagnosis of prostate cancer

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Journal of Hainan Medical University 2018年第20期24卷 69-71页

作者： Hua Xin Li Wang Shu-Ping Zhang Wen-Lu Zhang Hong-Xiang Mu Qing-Lin Jiang The First Affiliated Hospital of Jiamusi University Jiamusi154003HeilongjiangChina

Objective:To explore the combined application of multiple markers for diagnosis of prostate ***:To choose the hospital in September, 2015 to September 2012, initially determined both by serum PSA test for prostate cancer patients 117 cases, for patients with confirmed will be divided into three groups (39 cases further examination, compared with the pathological diagnosis of three groups of patients with prostate cancer diagnosis accuracy. Results: The detection rate in group A patients compared with the pathological diagnosis coincidence rate was 77.2%, B group was 90.3%, and 98.2% of group C, A, B two groups were lower in group C, group A and group B was higher than that that P504s inspection diagnosis rate is higher than about, CK34 beta E12 at 11:45, joint detection and diagnosis rate is highest. Three groups of patients were compared with difference had statistical significance . A group of 13 cases of benign specimen, 10 cases were negative, false positive in 3, coincidence rate was 76.9%, B group of 12 cases of benign specimens, the negative in 11 cases, false positives in 1 case, the coincidence rate is 91.7%, group C of 11 cases of benign specimens, negative in 11 cases, false positive 0 cases, coincidence rate was 100%. Combined detection of false positive diagnosis rate was lower than that in group A, B two patients, three groups of patients were compared with difference had statistical significance. Group A in 26 cases of prostate cancer, positive 22 cases, 4 cases were missed diagnosis coincidence rate was 84.6%. Group B in 27 cases of prostate cancer, positive 25 cases, 2 cases of misdiagnosis, the coincidence rate is 92.6%, group C in 27 cases of prostate cancer, 26 patients, positive, 1 case of misdiagnosis, the coincidence rate was 96.3%. Three groups of patients were compared with difference had statistical ***: Combination of P504s (AMACR), basal cell specific markers (about and CK34 beta E12 at 11:45) detection diag

关键词： Prostate cancer (PCa) A formyl Kiev enzyme a racemase (AMACR) P63 CK34βE12

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