检索结果-南通市图书馆

Different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第2期15卷 315-323页

作者： Shuai Wei Xue-Zhen Liang Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang The First Affiliated Hospital of Medical College Shihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina Institute of Orthopedics Chinese PLA General HospitalBeijingChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina The First Clinical Medical School Shandong University of Traditional Chinese MedicineJinanShandong ProvinceChina Kunming Medical University KunmingYunnan ProvinceChina Shanxi Medical University TaiyuanShanxi ProvinceChina

Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between s

关键词： gene ontology Kyoto Encyclopedia of Genes and Genomes isobaric tags for relative and absolute quantitation motor nerve proteomics sensory nerve spinal nerve Wallerian degeneration

Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2020年第2期15卷 302-310页

作者： Xian-Zhe Dong Dong-Xiao Wang Tian-Yi Zhang Xu Liu Ping Liu Yuan Hu Department of Clinical Pharmacology and Pharmacy Center of PharmacyChinese PLA General HospitalBeijingChina Department of Pharmacy Xuanwu Hospital of Capital Medical UniversityBeijingChina

Kai-Xin-San consists of Ginseng Radix, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria at a ratio of 3:3:2:2. Kai-Xin-San has been widely used for the treatment of emotional disorders in China. However, no studies have identified the key proteins implicated in response to Kai-Xin-San treatment. In this study, rat models of chronic mild stress were established using different stress methods over 28 days. After 14 days of stress stimulation, rats received daily intragastric administrations of 600 mg/kg Kai-Xin-San. The sucrose preference test was used to determine depression-like behavior in rats, while isobaric tags were used for relative and absolute quantitation-based proteomics to identify altered proteins following Kai-Xin-San treatment. Kai-Xin-San treatment for 2 weeks noticeably improved depression-like behaviors in rats with chronic mild stress. We identified 33 differentially expressed proteins: 7 were upregulated and 26 were downregulated. Functional analysis showed that these differentially expressed proteins participate in synaptic plasticity, neurodevelopment, and neurogenesis. Our results indicate that Kai-Xin-San has an important role in regulating the key node proteins in the synaptic signaling network, and are helpful to better understand the mechanism of the antidepressive effects of Kai-Xin-San and to provide objective theoretical support for its clinical application. The study was approved by the Ethics Committee for Animal Research from the Chinese PLA General Hospital(approval No. X5-2016-07) on March 5, 2016.

关键词： brain-derived neurotrophic factor signal pathway depression isobaric tags for relative and absolute quantitation Kai-Xin-San neurogenesis protein network proteomics analysis synaptic plasticity traditional Chinese medicine

Ultrasensitive quantitation of MicroRNAs via magnetic beads-based chemiluminesent assay

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2016年第8期59卷 1051-1058页

作者： Bingcong Zhou Haowen Yang Yan Deng Ming Liu Bin Liu Nongyue He Zhiyang Li State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Department of Biomedical Engineering School of Basic Medical Sciences Nanjing Medical University Department of Laboratory Medicine Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University

MicroRNAs(miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level, and their aberrant expression occurs during the development of malignant diseases. Recently, miRNAs have been proposed as potential prognostic and predictive biomarkers for early diagnosis. However, a major obstacle in rapid miRNA analysis from real samples is the lack of ultrasensitive and quantitative techniques. In this regard, the use of chemiluminescence(CL) system offers a highly sensitive strategy for detecting miRNAs. In this article, an ultrasensitive approach has been established for the quantification of miRNAs, using magnetic beads(MBs) and alkaline phosphatase(AP)-based CL system. This technique depends on sandwich hybridization among MBs-labeled capture probes, target miRNAs and biotin-labeled reporter probes, conjugation of streptavidin-alkaline phosphatase(SA-AP) to biotin-labeled reporter probes, and CL detection of AP-linked targets. Detection of miR-21 with this technique demonstrated a high selectivity and an ultralow limit of detection(LOD) of 60 fM with an extraordinarily wide range of six orders of magnitudes. The quantitation could be achieved by direct detecting target miRNA in serum samples within a total time of 1.5 h and did not require reverse transcription and polymerase chain reaction(PCR)amplification. Therefore, this developed method shows great potential for early cancer diagnosis based on miRNAs as biomarkers.

关键词： MicroRNA quantitation magnetic bead chemiluminescence liver cancer

quantitation of nucleoprotein complexes by UV absorbance and Bradford assay

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2021年第6期7卷 429-436页

作者： Jiang Chen Hao Luo Mei Tao Zhongchuan Liu Ganggang Wang Key Laboratory of Environmental and Applied Microbiology Chengdu Institute of Biology Chinese Academy of SciencesKey Laboratory of Environmental Microbiology of Sichuan Province College of Life Sciences Sichuan University University of Chinese Academy of Sciences

Despite the importance of studying nucleoprotein complexes, no appropriate method for quantifying them is available. Here, a UV absorbance method using the formula “C= 1.55 A– 0.76 A” were applied to quantify nucleoprotein complexes. After modification using two paired A260 and A280 values,the UV-derived formula-based method could accurately quantify proteins in nucleoprotein complexes.Otherwise, by taking the target protein as a standard, the Bradford assay can accurately quantify proteins in nucleoprotein complexes without interference by nucleic acids. The above methods were successfully applied to measure the concentration of Mtu P49-CTG complexes of Mycobacterium tuberculosis. In conclusion, both the Bradford assay and the UV-derived formula-based method were appropriate for quantifying proteins in nucleoprotein complexes, which may make contributions to explore the interactions between proteins and nucleic acids at the molecular level.

关键词： Nucleoprotein complex quantitation Bradford assay UV-derived formula-based method

Preparation and Application of Silica-based Perfusion Packing of Size Exclusion Chromatography for quantitation of Polyacrylamide in Enhanced Oil Recovery Systems

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Petroleum Science 2007年第2期4卷 82-90页

作者： Sui Xihua Zha Qingfang Wu Mingbo Guo Yansheng State Key Laboratory of Heavy Oil Processing China University of Petroleum （East China） Dongying Shandong 257061 China

The synthesis and characterization of a novel macroporous silica derived size exclusion chromatography （SEC） packing for quantitative analysis of high molecular weight （MW） polyacrylamide （PAM） are presented. Using this packing, a fast, sensitive and reproducible approach for quantitation of super high-MW PAM in demanding enhanced oil recovery （EOR） waters was developed and the effect of synthesis parameters on the properties of resultant materials was investigated. These parameters include salt addition, reaction temperature and duration, activation condition of functional groups on the silica surface, as well as the reaction cycles required for optimal silica modification. Moreover, SEC analysis conditions, such as mobile phase composition, flow rate, detection and sample preparation, were also explored and an optimal analysis protocol was developed. Under this optimized SEC analysis conditions, the synthesized macroporous materials proved satisfactory for quantification of PAM with average MW up to 22 million Daltons. An SEC analysis required less than few minutes with a detection limit of 1 ng, a linear response range of 0.1 to 75 mg/L with squared R value of 0.99 and reproducibility better than 9.2% RSD （relative standard deviation）. The analysis of PAM in highly saline oilfield production water containing interfering high MW polymeric surfactants indicated the recovery ranges from 92.5% to 110.1% for 1.0 mg/L PAM and 94.2% to 103.8% for 50 mg/L PAM solution. This study presented for the ftrst time that the reliable quantization of high MW PAM in highly demanding EOR waters can be achieved by SEC.

关键词： Macroporous silica size exclusion chromatography quantitation polyacrylamide

Ultra-High-Performance Liquid Chromatograph with Triple-Quadrupole Mass Spectrometer quantitation of Twelve Phenolic Components in Different Parts of Sarcandra glabra

在线全文

学校读者我要写书评

暂无评论

World Journal of Traditional Chinese Medicine 2021年第1期7卷 86-96页

作者： Jing-Guang Lu Cai-Yun Wang Da-Xin Chen Jing-Rong Wang Kai-Si Che Ming Zhong Wei Zhang Zhi-Hong Jiang State Key Laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacao Fujian Key Laboratory of Integrative Medicine on Geriatric Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Guangxi Institute of Chinese Medicine and Pharmaceutical ScienceNanningPeople’s Republic of China

Objective:The study objective was to determine phenolic components for the quality control(QC)of cultivated Sarcandra glabra(Thunb.)Makino(S.glabra).Materials and Methods:A sensitive,ultra-high-performance liquid chromatography-tandem mass spectrometric method for the simultaneous determination of 12 phenolic components has been developed.Six caffeoylquinic acids,two caffeoylshikimic acids,and four flavanonol glucosides were selected for the comprehensive analysis of distribution in different parts(root,stem,and leaf).Results:Twelve phenolic components were linear in the concentration range of 0.005–5.0μg/mL(R^(2)>0.995).The relative standard deviation of intra-day and inter-day precision across three validation runs over the entire concentration range was<5%.The recovery determined was within 5%in terms of relative error.Our results showed that the 12 phenolic compounds were mainly distributed in leaves and stems far more than those in roots.Conclusions:This study provided an ultra-high-performance liquid chromatograph with triple-quadrupole mass spectrometer quantitative method of 12 phenolic components for the QC of cultivated S.glabra.It was found that the phenolic components were significantly accumulated in the aerial parts(stems and leaves)of cultivated S.glabra.

关键词： Cultivation quantitation Sarcandra glabra ultra-high-performance liquid chromatograph with triple-quadrupole mass spectrometer

Non-target Screening and quantitation of Organic Chlorides in Oilfield Chemicals with Comprehensive Two Dimensional Gas Chromatography Coupled with Time of Flight Mass Spectrometry

在线全文

学校读者我要写书评

暂无评论

China Petroleum Processing & Petrochemical Technology 2017年第4期19卷 65-71页

作者： Zheng Kewen Guo Rui Ren Guofa Xia Lingyan Xiong Wei Zhang Jin Chu Xiaodong Yu Zhiqiang Institute of Environmental Pollution and Health School of Environmental and Chemical Engineering Shanghai University Shanghai 200072 SINOPEC Shengli Oilfield Branch Dongying 257000

Advanced analytical methodology based on comprehensive two-dimensional gas chromatography-time of flight mass spectrometry(GC×GC-TOFMS) is proposed for investigation of organic chlorides in different oilfield chemicals.The target screening was initially carried out on 8 suspected organic chlorides by evaluating the capability of the enhanced separation and reliable identification at a trace concentration. GC×GC-TOFMS allowed for the fast and automated analysis of organic chlorides at a level of 200 μg/L. This method was subsequently applied for non-target screening of organic chlorides in different oilfield chemicals at various locations across China. 22 organic chlorides were identified and verified by comparison with pure standards in the mixed sample. Finally, this method was used to determine the content of the organic chlorides in individual samples. The result showed that the organic chloride levels in 19 of the 39 tested oilfield chemicals were above the threshold limit of 1.0 mg/L.

关键词： GC×GC-TOFMS non-target screening oilfield chemicals organic chlorides quantitation

How to use open-pFind in deep proteomics data analysis?—A protocol for rigorous identification and quantitation of peptides and proteins from mass spectrometry data

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2021年第3期7卷 207-226页

作者： Guangcan Shao Yong Cao Zhenlin Chen Chao Liu Shangtong Li Hao Chi Meng-Qiu Dong School of Life Sciences Peking University National Institute of Biological Sciences Beijing Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua University Key Lab of Intelligent Information Processing of Chinese Academy of Sciences (CAS) University of CASInstitute of Computing TechnologyCAS University of Chinese Academy of Sciences Beijing Advanced Innovation Center for Big Data-Based Precision Medicine School of Medicine and EngineeringBeihang University

High-throughput proteomics based on mass spectrometry(MS) analysis has permeated biomedical science and propelled numerous research projects. p Find 3 is a database search engine for high-speed and in-depth proteomics data analysis. p Find 3 features a swift open search workflow that is adept at uncovering less obvious information such as unexpected modifications or mutations that would have gone unnoticed using a conventional data analysis pipeline. In this protocol, we provide step-by-step instructions to help users mastering various types of data analysis using p Find 3 in conjunction with p Parse for data pre-processing and if needed, p Quant for quantitation. This streamlined p Parse-p Findp Quant workflow offers exceptional sensitivity, precision, and speed. It can be easily implemented in any laboratory in need of identifying peptides, proteins, or post-translational modifications, or of quantitation based on15N-labeling, SILAC-labeling, or TMT/i TRAQ labeling.

关键词： Protein identification Mass spectrometry Search engine Open-pFind quantitation

Formation of a ZnO nanorods-patterned coating with strong bactericidal capability and quantitative evaluation of the contribution of nanorods-derived puncture and ROS-derived killing

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第5期7卷 181-191页

作者： Jing Ye Bo Li Mei Li Yufeng Zheng Shuilin Wu Yong Han State Key Laboratory for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an710049China Department of Orthopedics Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou510080China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing100871China School of Materials Science&Engineering The Key Laboratory of Advanced Ceramics and Machining Technology By the Ministry of Education of ChinaTianjin UniversityTianjin300072China

To endow Ti-based orthopedic implants with strong bactericidal activity,a ZnO nanorods-patterned coating(namely ZNR)was fabricated on Ti utilizing a catalyst-and template-free method of micro-arc oxidation(MAO)and hydrothermal treatment(HT).The coating comprises an outer layer of ZnO nanorods and a partially crystallized inner layer with nanocrystalline TiO_(2) and Zn_(2)TiO_(4) embedded amorphous matrix containing Ti,O and Zn.During HT,Zn^(2+)ions contained in amorphous matrix of the as-MAOed layer migrate to surface and react with OH􀀀in hydrothermal solution to form ZnO nuclei growing in length at expense of the migrated Zn^(2+).ZNR exhibits intense bactericidal activity against the adhered and planktonic S.aureus in vitro and in vivo.The crucial contributors to kill the adhered bacteria are ZnO nanorods derived mechano-penetration and released reactive oxygen species(ROS).Within 30 min of S.aureus incubation,ROS is the predominant bactericidal contributor with quantitative contribution value of ~20%,which transforms into mechano-penetration with prolonging time to reach quantitative contribution value of ~96% at 24 h.In addition,the bactericidal contributor against the planktonic bacteria of ZNR is relied on the released Zn^(2+).This work discloses an in-depth bactericidal mechanism of ZnO nanorods.

关键词： Anti-bacteria ZnO nanorods Hydrothermal treatment Formation mechanism Bactericidal contributors quantitation