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Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus

Journal of Oceanology and Limnology 2018年第6期36卷 2368-2378页

作者： DUAN Hu JIN Songjun LI Fuhua ZHANG Xiaojun XIANG Jianhai Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, diff erentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds:(1) surface glia;(2) cortex glia; and(3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, Ⅱ and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7–9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.

关键词： Cherax quadricarinatus brain’s structure brain cells primary culture

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Treatments for Temnocephalids Ectosymbiont Craspedella sp. on Cherax quadricarinatus and Cherax albertisii “Papua Freshwater Lobster”

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World Journal of Engineering and Technology 2015年第4期3卷 48-54页

作者： Desy Sugiani Angela Mariana Lusiastuti Taukhid Uni Purwaningsih Research Institute for Freshwater Aquaculture Ministry of Marine Affairs and Fisheries Bogor Indonesia

There were two species of crayfish “red-claw” (Cherax quadricarinatus) and “blue-huna” (Cherax albertisii) for their aquaculture potential. Crayfish were susceptible to fungal (crawfish plague), parasitic (protozoa and nematodes), and bacterial pathogen. A number of ectosymbiont Craspedella sp. have been observed on red-claw and blue-huna. The flatworms were commonly found almost in the whole body, on the upper exoskeleton behind the head, in the gill cavity and on the claws and underside of crayfish. Although their number sometimes was very high, they didn’t cause any problems especially for the new molting crayfish. Micro organisms living on the crayfish surface body and worms didn’t cause any pathological changes. Adults Craspedella sp. can be eliminated by a short bath in salt water or formaldehyde 37% solutions for several hours. This treatment didn’t kill worm eggs, so it needs to be repeated every one week. Moreover, hyposalinity or OST (Osmotic Shock Therapy) is one of the most effective therapies for ectoparasites on Craspedella sp. with dose of bath treatment 15 grams per litre of salt (15 ppt) for more than 3 hours, and dipped in salt water at 30 ppt (or 3.0 ppm, seawater salinity) for 15 - 20 minutes.

关键词： Craspedella sp. Cherax quadricarinatus Cherax albertisii Treatment

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循环水育苗系统各水处理单元水质变化比较

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湖北农业科学 2018年第2期57卷 95-97,105页

作者：李倩周志明王雨辰郭建林林锋胡廷尖浙江省淡水水产研究所/农业部淡水渔业健康养殖重点实验室浙江湖州313001

2015年3-4月,结合红螯螯虾(Cherax quadricarinatus Von Martens)育苗周期,对温室育苗系统水处理单元的水质指标实施周期监测。结果表明,水源水质的总氮(TN)、总磷(TP)、氨氮(NH3-N)、亚硝酸盐(NO2-N)、硝酸盐(NO3-N)和高锰酸盐指数(COD... 详细信息

2015年3-4月,结合红螯螯虾(Cherax quadricarinatus Von Martens)育苗周期,对温室育苗系统水处理单元的水质指标实施周期监测。结果表明,水源水质的总氮(TN)、总磷(TP)、氨氮(NH3-N)、亚硝酸盐(NO2-N)、硝酸盐(NO3-N)和高锰酸盐指数(CODMn)均较低,符合养殖标准。在育苗期间,育苗池进水口的TN、TP、NO2-N和NO3-N的平均值最高,分别为6.2、0.76、0.035、4.2 mg/L;袋式过滤器的NH3-N浓度最高,平均为0.23 mg/L,各处理单元的CODMn值差异不大。随着育苗时间的延长,育苗水体各指标均有不同程度的升高。从处理单元分析,经处理后,水质指标明显改善,保证了育苗过程的顺利进行。

关键词：循环水系统红螯螯虾(Cherax quadricarinatus Von Martens) 育苗水处理

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