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Antihypertensive Properties on Spontaneously Hypertensive Rats of Peptide Hydrolysates from Silkworm pupae Protein

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Food and Nutrition Sciences 2014年第13期5卷 1202-1211页

作者： Wei Wang Nan Wang Yu Zhang College of Biology and Environmental Engineering Zhejiang Shuren University Hangzhou China Institute of Quality and Standard for Agriculture Products Zhejiang Academy of Agricultural Science Hangzhou China

Peptide hydrolysates of silkworm pupae protein with molecular weight of less than 5000 Da were prepared by ultrafiltration. The extracted peptide hydrolysates of silkworm pupae protein had inhibitory action on angiotensin-I-converting enzyme activity in vitro. The hydrolysates were orally administered to spontaneously hypertensive rats (SHR) in one period and long-term (four weeks). The results showed that the systolic blood pressure (SBP) of the treatment groups decreased in a dose-related manner. After one oral administration of silkworm protein hydrolysates with doses of 60, 20 and 5 mg/kg, the SBP of SHR decreased by 21.5, 13.8, and 9.0 mmHg in 1.5 h. After four weeks of the treatment in 80 mg/kg, the SBP decreased by 25 mmHg, with the antihypertensive activity close to 4 mg/kg of captopril;the SBP of the 40 mg/kg dose group also decreased by 17.5 mmHg. The peptide hydrolysate did not affect the SBP in normal, non-hypertensive rats in one period and long-term treatments. The acute toxicity research showed that the peptide hydrolysates were safe and without side effects. This research would be helpful in exploring the silkworm protein peptides as functional components for the antihypertension treatment.

关键词： Angiotensin-I-Converting Enzyme Antihypertensive Effect Hypertension Systolic Blood Pressure Silkworm pupae Hydrolysate Spontaneously Hypertensive Rat

Developmental Threshold Temperature and Effective Accumulative Tem- perature of pupae and Eggs of Holcocerus hippophaecolus

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Forestry Studies in China 2004年第4期6卷 34-38页

作者： ZongShixiang LuoYouqing WangTao XuZhichun LuChangkuan CollegeofResourcesandEnvironment BeijingForestryUniversityBeijing100083P.R.China

In order to understand the occurrence and the developmental regularity of seabuckthorn carpenterworm (Holcocerus hippophaecolus) and predict its population density, the developmental threshold temperature (C) and effective accumulative tem- perature (K) of the carpenterworm pupae and eggs were analyzed under the conditions of constant and variable temperatures. The results show that the values of C and K of the carpenterworm pupae are (12.1 ± 0.2) °C and (295.2 ± 4.1) day-degrees at constant temperatures, and (15.5 ± 0.4) °C and (202.4 ± 13.1) day-degrees at variable temperatures. However, the values of C and K of the eggs at variable temperatures are (16.7 ± 0.8) °C and (101.5 ± 12.6) day-degrees. The differences of developmental threshold and effective accumulative temperature under the conditions of constant and variable temperatures of the carpenterworm pupae accord with the developmental regularity of most insects in nature. By comparing five different constant temperatures, the conclusion is that the optimum developmental temperature of the pupae is 21 °C when both the pupation of the mature larvae and the eclosion of the pupae are very accordant. Moreover, the percentage of eclosion is over 90%. The average developmental durations of the carpenter- worm pupae and eggs are 31 and 16 d at variable temperatures.

关键词： Holcocerus hippophaecolus pupae eggs developmental threshold temperature effective accumulative tempera- ture

INHIBITION EFFECTS OF ANTIBACTERIAL PEPTIDE D FROM THE CHINESE OAK SILKWORM(Antheraea pernyi) pupae

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Chinese Science Bulletin 1987年第9期32卷 629-632页

作者：黄自然郑庭辉梁懿彰屈贤铭 Department of Sericulture South China Agricultural University Guangzhou First Affiliated Hospital Zhongshan Medical University Guangzhou Shanghai Institute of Biochemistry Academia Sinicaome active antibacterial substances such as antibacterial peptide lysozymes and agglutinin were induced and developed from the haemolymph of the Chinese oak silkworm(Antheraea pernyi) pupae injected with Escherichia coli or treated with ultrasonic waves. The antibacterial peptide from A. pernyi was isolated purified and its amino acid sequence was also determined. The inducible antibacterial substances have marked inhibition

Some active antibacterial substances, such as antibacterial peptide lysozymes and agglutinin were induced and developed from the haemolymph of the Chinese oak silkworm(Antheraea pernyi) pupae injected with Escherichia coli or treated with ultrasonic waves. The antibacterial peptide from A. pernyi was isolated, purified, and its amino acid sequence was also determined. The inducible antibacterial substances have marked

关键词： pernyi Antheraea pupae antibacterial silkworm agglutinin marked ultrasonic purified injected

Comparison of Nucleic Acid Content among Non-diapause pupae, Diapause pupae and Eclosion-adult from Diapause pupae of Papilio memnon

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Agricultural Science & Technology 2009年第3期10卷 101-103,107页

作者：易传辉陈晓鸣史军义周成理中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室云南昆明650224 云南林业职业技术学院云南昆明650224

[Objective] The aim of this study was to provide basis for deeply understanding the diapause mechanism of Papilio memnon L. [Method] RNA and DNA content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different development stages were detected by the colorimetry. [Result] RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg, while diapause pupae was 4.326 0-5.885 3 μg/mg and eclosion-adult from diapause pupae was 20.779 3 μg/mg at initial stage. DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg, while diapause pupae was 0.452 0-0.828 3 μg/mg and eclosion-adult from diapause pupae was 1.727 0 μg/mg at initial stage. [Conclusion] The nucleic acid content and change is related to the development stage.

关键词： Papilio memnon Nucleic acid content Diapause pupae Adult

Comparison of Protein Content among Non-diapause pupae,Diapause pupae and Eclosion-adult from Diapause pupae of Papilio memnon

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Agricultural Science & Technology 2009年第2期10卷 127-129页

[ Objective] The experiment aimed to explore the diapause mechanism of Papilio memnon L. further. [ Method] The colorimetry method was used to detect protein content of non-diapause pupae, diapause pupae and eclosion-adult from diapause pupae at different developmental stages. [Result] It was showed that the protein content of non-diapause pupae was from 111.768 to 115.030 7 mg/g and the change of protein content during whole pupal stage, while diapause pupae were from 81.218 to 131.623 7 mg/g and the change of protein content was not big from last ten-day period of October to last ten-day pariod of November, however, the content increased from the last ten-day period of November. The protein content of emerging adult was 296.969 3 mg/g. [Conclusion] The change of protein content was related to Papilio memnon at different development stage.

关键词： Papilio memnon Protein content Diapause pupae Dault

Pupal emergence pattern in cactophilic Drosophila and the effect of host plants

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Insect Science 2018年第6期25卷 1108-1118页

作者： Eduardo M.Soto Julián Padrò Pablo Milla Carmona Diego T.Tuero Valeria P.Carreira Ignacio M.Soto Departamento de Ecologia Genética y Evoluciòn Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresIEGEBA-CONICETBuenos AiresArgentina

Drosophila buzzatii and *** are sibling cactophilie *** former breeds primarily on prickly pears (genus Opuntia)whereas the latter breeds on columnar cacti of the genera Cereus and Trichocereus,although with certain degree of niche *** examined the interspecific differences in diurnal temporal patterns of adult emergence from puparia and evaluated whether this behavior is affected by rearing in the different cactus hosts available in *** detected important host-dependent genetic variation for this trait differentially affecting the emergence schedule of these *** pattern of emergence time was directly correlated with developmental time and negatively correlated with adult wing size,suggesting that early emergences are at least indirectly correlated with increased *** discussed our results in terms of their putative effects on fitness and the genetic-metabolic pathways that would be presumably affected by host's nutritional-chemical differences.

关键词： alkaloids cactus circadian cycle life history traits phenotypic plasticity pupae

Effect of the Variety of Fig Tree on Some Biological Parameters of Ceratitis capitata Wied, 1824 （Diptera： Trypetidae） in Some Orchards in the Kabylie

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Journal of Life Sciences 2012年第3期6卷 312-319页

作者： Sadoudi-Ali Ahmed Djamila Nabila Rezoug Ferroudja Saiki Noreddine Soltani M. Mammeri University Tizi-Ouzou 15000 Algeria Dadji Mokhtar University Annaba 23000 Algeria

The Mediterranean fruit fly, Ceratitis capitata Wied. （1824） is one of the most important pest that can infest orchards if its spread is not controlled. Among the preventive measures recommended in the fight against this pest, we are interested in looking for varieties that are less favorable to the development of this pest among different varieties of fig trees in our region. The biological parameters of the fruit fly which were followed in this study are duration of pupation, rate of emergence, sex ratio and longevity of males and females. The results show that these parameters vary depending on the variety of fruit.

关键词： Ceratitis capitata varieties of figs pupae pupation emergence sex ratio longevity.

Effective accumulated temperature and developmental threshold temperature for Semanotus bifasciatus(Motschulsky) in Beijing

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Forest Ecosystems 2008年第2期10卷 125-129页

作者： MA Li-qin1,GAO Shi-ji2,WEN Jun-bao1,ZONG Shi-xiang1,XU Zhi-chun1 1Key Laboratory for Silviculture and Conservation of Ministry of Education,Beijing Forestry University,Beijing 100083,P.R.China 2Beijing Agricultural Vocational College,Beijing 100088,P.R.China Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University Beijing P. R. China Beijing Agricultural Vocational College Beijing P. R. China

In order to understand the occurrence and development of Semanotus bifasciatus(Motschulsky),a borer insect of Platy-cladus orientalis in parts of northern and eastern China and to improve its prediction,the developmental threshold temperature(Ct) and effective accumulated temperature(K) of *** were determined under conditions of constant and variable *** results show that under constant temperatures the value of Ct has a range of 8.90±1.77°C and the value of K lies between 95.19±13.14 degree-day for eggs;the values for larvae are Ct = 13.26±3.06°C and the number of degree-day is K = 2,885.07±187.87 degree-day;for pupae the ranges are Ct = 8.93±2.49°C and K = 131.20±25.63 *** the condition of ambient tempera-tures,the values for eggs are Ct = 17.33±1.24°C and K = 70.79±8.99 *** is suggested that *** adults over-winter in a state of dormancy,not at a *** winter may accelerate the life cycle of *** can be used to predict an early occurrence of ***.

关键词： Semanotus bifasciatus(Motschulsky) developmental threshold temperature effective accumulated temperature eggs larvae pupae

Effects of Sublethal Doses of Chlorfluazuron on Pupal-testis in the Common Cutworm, Spodoptera fitura （F,）（Lepidoptera： Noctuidae）

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Journal of Agricultural Science and Technology(A) 2011年第6期1卷 890-896页

作者： F. Perveen S. A. Mehmood S. Yasmin Department of Zoology Hazara University Garden Campus Mansehra 21300 Pakistan

The effects of sublethal doses （LD10：1.00 ng larval-1; LD30： 3.75 ng larva-1） of chlorfluazuron on number and size of developmental spermatocytes in pupal-testis during spermatogenesis of the common cutworm, Spodoptera litura （F.） are described. Chlorfluazuron was applied topically to newly-ecdysed fifth-instar larvae of S. litura under laboratory conditions. During first day of pupation, spermatids were the highest （3,430 ± 7）, however, elongated spermatocytes with mature sperm were the lowest （292 ± 16） in number in pupal-testis compared with other developing spermatocytes. During last day of pupation, elongated spermatocytes with mature sperm were the highest （3,581 ± 3）, however, spermatogonia were the lowest （86± 4） in number in pupal-testis compared with other developing spermatocytes. The number of spermatogonia, primary and secondary spermatocytes, spermatids and elongated cysts with mature sperm were increased during pupal-developmental days in the controls. However, their numbers were significantly reduced in LD10- and even more significantly reduced in LD30-treated pupal-testis compared with controls. Moreover, eupyrene sperm bundles were not present in pupal-testis. During pupal-developmental days, spermatids were the smallest （4.1 ± 0.9 dm in μm） and secondary spermatocytes were the largest （31.0 ± 1.8 dm in μm） in size compared with other developing spermatocytes. The sizes of the same developing spermatocytes were significantly reduced in LD10-treated and more significantly reduced in LD30-treated pupal-testis of S. litura compared with controls.

关键词： Chlorfluazuron developing spermatocytes pupae spermatogenesis Spodoptera litura sublethal doses.