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Efficiency of Oryza punctata extract on glucose regulation: Inhibition of α-amylase and α-glucosidase activities

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Grain & Oil Science and Technology 2019年第2期2卷 44-48页

作者： Balasubramaniam Jaya Prasad Pazhaniyandi Subramania Sharavanan Rengaraj Sivaraj Department of Botany Annamalai UniversityAnnamalai Nagar 608002India Department of Botany Govt Arts CollegeMannargudi 614001India Department of Pharmacology Aarupadai Veedu Medical College&HospitalPuducherry 607403India

Red rice(Oryza punctata) is a type of unpolished rice which has higher nutritional value compared to white rice or even polished rice. Owing to higher nutritive content and metabolites, dieticians strongly advise red rice for peoples with metabolic disorders including diabetics. However, the mechanism of action and contents of secondary metabolites in Indian red rice variety not reported scientifically. Therefore, the present study aimed to evaluate its mechanism of action through inhibitory effect of α-amylase and α-glucosidase. Initially, the whole grain of red rice was macerated with methanol at room temperature for 2 weeks. Then, the dried and powdered, samples at different concentrationfi(2.5, 10, 40, and 80 μg/m L) were employed to nd out in vitro inhibitory effects on α-amylase and α-glucosidase. In addition, an enzyme kinetics of effective extract was calculated by Line-weaver Burk(LWB) plot analysis. Moreover,the valuable metabolites in the efficient methanolic extract were quantified using high performance liquid chromatography(HPLC). The results demonstrated that red rice methanolic extract(RRMEt) possess strong inhibitory activity onα-amylase and α-glucosidase compared with acarbose(P < 0.01). The IC50 values of RRMEt was found to be 29.7 ±7.43 μg/m L for α-amylase and 20.4 ± 0.25 μg/m L for α-glucosidase. LWB indicated that RRMEt is an uncompetitive inhibitor. Further, HPLC analysis revealed protocatechuic acid, catechin, and chlorogenic acids were more abundant in RRMEt among the fourteen metabolites. We conclude, the efficiency of enzyme inhibition through the influence of phenolic compounds in RRMEt.

关键词： Oryza punctata α-Amylase α-Glucosidase High performance liquid chromatography (HPLC) Pigmented rice Catechin

Donors for Resistance to Brown Planthopper Nilaparvata lugens(St?l) from Wild Rice Species

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Rice science 2016年第4期23卷 219-224页

作者： Preetinder S. SARAO Gurpreet K. SAHI Kumari NEELAM Gurjit S. MANGAT Bhaskar C. PATRA Kuldeep SINGH Department of Plant Breeding and Genetics Punjab Agricultural University Ludhiana Punjab 141004 India School of Agricultural Biotechnology Punjab Agricultural University Ludhiana Punjab 141004 India Crop Improvement Division ICARNational Rice Research Institute Cuttack 753006 India

Out of 1 989 wild accessions sown in seed boxes for screening, only 1 003 wild accessions with good germination were screened against brown planthopper(BPH), Nilaparvata lugens(St?l) under greenhouse conditions. The collection comprised of accessions from 11 wild species and African cultivated rice. The germplasm was screened for BPH following standard seed box screening technique in the greenhouse. As many as 159 accessions were identified as resistant during the year 2012 based on one year screening. A selected set of BPH resistant accessions were screened again during 2013. Based on the two years screening, seven accessions of O. nivara(AA), one accession of O. officinalis(CC), seven accessions of O. australiensis(EE), five accessions of O. punctata(BB and BBCC) and nine accessions of O. latifolia(CCDD) were confirmed to be resistant to BPH. So far no BPH resistance genes have been identified and designated from O. nivara and O. punctata, hence these may act as new sources of resistance.

关键词： brown planthopper O. nivara O. officinalis O. australiensis O. punctata resistance rice

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金鱼致病菌的分离鉴定与药敏特性

淡水渔业 2010年第6期40卷 46-49,55页

作者：曹海鹏刘丽玲李怡杨先乐上海海洋大学国家水生动物病原库上海201306

采用常规方法对患病金鱼(Carassius auratus)内脏组织(肝脏、肾脏、性腺)中致病菌进行了分离鉴定与药敏特性研究。结果显示:实验共分离了8株优势菌,经人工回归感染实验证实其中3株细菌(菌株LK-T、PL-R和PK-T)具有致病性。ATB细菌鉴定仪... 详细信息

采用常规方法对患病金鱼(Carassius auratus)内脏组织(肝脏、肾脏、性腺)中致病菌进行了分离鉴定与药敏特性研究。结果显示:实验共分离了8株优势菌,经人工回归感染实验证实其中3株细菌(菌株LK-T、PL-R和PK-T)具有致病性。ATB细菌鉴定仪鉴定结果显示,菌株LK-T和菌株PL-R为温和气单胞菌(Aeromonas so-bria),菌株PK-T为豚鼠气单胞菌(Aeromonas punctata ***)。这3株致病菌的主要特征为:革兰氏染色阴性,杆状,对奈替米星、头孢呋肟、头孢拉啶、庆大霉素、环丙沙星、新霉素、先锋必等药物均高度敏感。其中庆大霉素、环丙沙星、新霉素等常规渔药可作为防治用药的依据。

关键词：金鱼(Carassius auratus) 温和气单胞菌(Aeromonas sobria) 豚鼠气单胞菌(Aeromonas punctata subsp.caviae) 药敏特性

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鲟细菌性败血综合征致病菌的初步研究

淡水渔业 2007年第2期37卷 53-56页

作者：曹海鹏杨先乐高鹏李怡张书俊邓璐农业部渔业动植物病原库上海水产大学上海200090

从患细菌性败血综合征的杂交鲟(Huso huso♀×Acipenser ruthenus ♂)和达氏鳇(Huso dauricus)的腹水及肾脏中共分离了5株优势菌,经人工回归感染实验证实其中2株细菌XL2-T和HK3-R具有致病性。经ATB细菌鉴定仪鉴定,结果显示:XL2-T为豚鼠... 详细信息

从患细菌性败血综合征的杂交鲟(Huso huso♀×Acipenser ruthenus ♂)和达氏鳇(Huso dauricus)的腹水及肾脏中共分离了5株优势菌,经人工回归感染实验证实其中2株细菌XL2-T和HK3-R具有致病性。经ATB细菌鉴定仪鉴定,结果显示:XL2-T为豚鼠气单胞菌(Aeromonas punctata ***),HK3-R为类志贺邻单胞菌(Plesiomonas shigelloides)。这2株致病菌的主要特征为:革兰氏染色阴性,杆状,氧化酶阳性,对先锋必、妥布霉素、头孢曲松、头孢噻肟、奈替米星、新霉素等6种药物均高度敏感。

关键词：杂交鲟(Huso huso ♀×Acipenser ruthenus♂) 达氏鳇(Huso dauricus) 细菌性败血综合征豚鼠气单胞菌(Aeromonas punctata subsp.caviae) 类志贺邻单胞菌(Plesiomonas shigelloides)