检索结果-南通市图书馆

pseudomonas Cyclic Lipopeptide Medpeptin:Biosynthesis and Modulation of Plant Immunity

Engineering 2023年第9期28卷 153-165页

作者： Yi-Lin Gu Jun-Zhou Li Yan Li Shen Cong Jing Wang Yi-Nan Ma Hai-Lei Wei State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Key Laboratory of Microbial Resources Collection and PreservationMinistry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China

The multifunctional secondary metabolites known as cyclic lipopeptides(CLPs),which are produced by a large variety of bacteria,have become a key category of plant immunity elicitors.pseudomonas-CLPs(PsCLPs)are extremely diverse in structure and biological activity.However,an understanding of CLP-plant structure–function interactions currently remains elusive.Here,we identify medpeptin,a novel CLP from pseudomonas mediterranea that consists of 22 amino acids.Medpeptin is synthesized by a non-ribosomal peptide synthase(NRPS)gene cluster and regulated by a quorum-sensing system.Further research indicates that medpeptin does not exhibit antimicrobial activity;instead,it induces plant cell death immunity and confers resistance to bacterial infection.Comparative transcriptome analysis and virus-induced gene silencing(VIGS)reveal a set of immune signaling candidates involved in medpeptin perception.Silencing of a cell-wall leucine-rich repeat extensin protein(NbLRX3)or a receptor-like protein kinase(NbRLK25)—but not BAK1 or SGT1—compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana benthamiana.Our findings point to a noncanonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance.

关键词： pseudomonas Cyclic lipopeptide Cell death Leucine-rich repeat extension(LRX) Medpeptin Receptor-like kinase(RLK)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microbial production of cis,cis-muconic acid from aromatic compounds in engineered pseudomonas

引用

Synthetic and Systems Biotechnology 2023年第3期8卷 536-545页

作者： Siyang He Weiwei Wang Weidong Wang Haiyang Hu Ping Xu Hongzhi Tang State Key Laboratory of Microbial Metabolism and School of Life Sciences&BiotechnologyShanghai Jiao Tong UniversityShanghai200240People’s Republic of China College of Life Science Northeast Forestry UniversityHarbin150040China

Industrial expansion has led to environmental pollution by xenobiotic compounds like polycyclic aromatic hydrocarbons and monoaromatic hydrocarbons.pseudomonas spp.have broad metabolic potential for degrading aromatic compounds.The objective of this study was to develop a“biological funneling”strategy based on genetic modification to convert complex aromatic compounds into cis,cis-muconate(ccMA)using pseudomonas putida B6-2 and P.brassicacearum MPDS as biocatalysts.The engineered strains B6-2(B6-2ΔcatBΔsalC)and MPDS(MPDSΔsalC(pUCP18k-catA))thrived with biphenyl or naphthalene as the sole carbon source and produced ccMA,attaining molar conversions of 95.3%(ccMA/biphenyl)and 100%(ccMA/naphthalene).Under mixed substrates,B6-2ΔcatBΔsalC grew on biphenyl as a carbon source and transformed ccMA from non-growth substrates benzoate or salicylate to obtain higher product concentration.Inserting exogenous clusters like bedDC1C2AB and xylCMAB allowed B6-2 recombinant strains to convert benzene and toluene to ccMA.In mixed substrates,constructed consortia of engineered strains B6-2 and MPDS specialized in catabolism of biphenyl and naphthalene;the highest molar conversion rate of ccMA from mixed substrates was 85.2%when B6-2ΔcatBΔsalC was added after 24 h of MPDSΔsalC(pUCP18k-catA)incubation with biphenyl and naphthalene.This study provides worthwhile insights into efficient production of ccMA from aromatic hydrocarbons by reusing complex pollutants.

关键词： Biodegradation Polycyclic aromatic hydrocarbons Biological funneling cis,cis-muconic acid pseudomonas

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strain pseudomonas PA1201

引用

Synthetic and Systems Biotechnology 2023年第4期8卷 618-628页

作者： Ying Cui Kai Song Zi-Jing Jin Learn-Han Lee Chitti Thawai Ya-Wen He State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai200240China Novel Bacteria and Drug Discovery Research Group(NBDD) Microbiome and Bioresource Research Strength(MBRS)Jeffrey Cheah School of Medicine and Health SciencesMonash University MalaysiaBandar SunwaySelangor Darul Ehsan47500Malaysia Department of Biology Faculty of ScienceKing Mongkut’s Institute of Technology LadkrabangBangkok10520Thailand

Biocontrol strain pseudomonas PA1201 produces pyoluteorin(Plt),which is an antimicrobial secondary metabolite.Plt represents a promising candidate pesticide due to its broad-spectrum antifungal and antibacterial activity.Although PA1201 contains a complete genetic cluster for Plt biosynthesis,it fails to produce detectable level of Plt when grown in media typically used for pseudomonas strains.In this study,minimum medium(MM)was found to favor Plt biosynthesis.Using the medium M,which contains all the salts of MM medium except for mannitol,as a basal medium,we compared 10 carbon sources for their ability to promote Plt biosynthesis.Fructose,mannitol,and glycerol promoted Plt biosynthesis,with fructose being the most effective carbon source.Glucose or succinic acid had no significant effect on Plt biosynthesis,but effectively antagonized fructose-dependent synthesis of Plt.Promoter-lacZ fusion reporter strains demonstrated that fructose acted through activation of the pltLABCDEFG(pltL)operon but had no effect on other genes of plt gene cluster;glucose or succinic acid antagonized fructose-dependent pltL induction.Mechanistically,fructose-mediated Plt synthesis involved carbon catabolism repression.The two-component system CbrA/CbrB and small RNA catabolite repression control Z(crcZ)were essential for fructose-induced Plt synthesis.The small RNA binding protein Hfq and Crc negatively regulated fructose-induced Plt.Taken together,this study provides a new model of fructose-dependent Plt production in PA1201 that can help improve Plt yield by biosynthetic approaches.

关键词： pseudomonas Biocontrol Pyoluteorin Fructose Carbon catabolism repression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

pseudomonas aeruginosa:Infections and novel approaches to treatment“Knowing the enemy”the threat of pseudomonas aeruginosa and exploring novel approaches to treatment

Infectious Medicine

引用

Infectious Medicine 2023年第3期2卷 178-194页

作者： Nikhil Sathe Peter Beech Larry Croft Cenk Suphioglu Arnab Kapat Eugene Athan Reliance Life Sciences Pvt.Ltd. Dhirubhai Ambani Life Sciences CentreThane Belapur RoadRabaleNavi Mumbai 400701India School of Life and Environmental Sciences Deakin UniversityMelbourne Burwood Campus221 Burwood HighwayBurwood Victoria 3125Australia NeuroAllergy Research Laboratory School of Life and Environmental SciencesDeakin UniversityGeelong Campus at Waurn Ponds75 Pigdons RoadWaurn Ponds Victoria 3216Australia School of Medicine Deakin UniversityPO Box 281 Geelong 3220Australia

pseudomonas aeruginosa is an aerobic Gram-negative rod-shaped bacterium with a comparatively large genome and an impressive genetic capability allowing it to grow in a variety of environments and tolerate a wide range of physical conditions.This biological flexibility enables the P.aeruginosa to cause a broad range of infections in patients with serious underlying medical conditions,and to be a principal cause of health care associated infection worldwide.The clinical manifestations of P.aeruginosa include mostly health care associated infections and community-acquired infections.P.aeruginosa possesses an array of virulence factors that counteract host defence mechanisms.It can directly damage host tissue while utilizing high levels of intrinsic and acquired antimicrobial resistance mechanisms to counter most classes of antibiotics.P.aeruginosa co-regulates multiple resistance mechanisms by perpetually moving targets poses a significant therapeutic challenge.Thus,there is an urgent need for novel approaches in the development of anti-pseudomonas agents.Here we review the principal infections caused by P.aeruginosa and we discuss novel therapeutic options to tackle antibiotic resistance and treatment of P.aeruginosa infections that may be further developed for clinical practice.

关键词： pseudomonas Infections Antibiotics Resistance Novel Approaches Bacteriophages Immunotherapy Vaccines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enhanced fat degradation following the addition of a pseudomonas species to a bioaugmentation product used in grease traps

引用

Journal of Environmental Sciences 2019年第3期31卷 174-188页

作者： Markella Tzirita Seraphim Papanikolaou Bríd Quilty School of Biotechnology Dublin City University (DCU) Department of Food Science and Human Nutrition Agricultural University of Athens National Institute for Cellular Biotechnology (NICB) DCU

The presence of fats, oils and greases(FOGs) in wastewater can lead to many problems including blockages. Investigation of a bioaugmentation product, consisting of Bacillus spp., to degrade butter(1%, W/V) and olive oil(1%, V/V) was performed in aerobic batch cultures for 13-day incubation. Gravimetric analysis of the remaining substrates showed slowly degradation of the oil after a 2-day lag, but no degradation of the butter. Addition of a pseudomonas putida strain CP1 to the Bacillus spp. population promoted rapidly degradation of both fats after7 days of incubation. High fat accumulation revealed the potential use of the new bacterial mixture for production of added-value compounds. Lipase production only by the Bacillus spp.along with the analysis of the remaining lipids with thin layer chromatography and gas chromatography, suggested that the Bacillus spp. mainly only hydrolyzed the fat. The breakdown products were metabolized by the pseudomonas putida CP1 performing preferential utilization of unsaturated fatty acids. Investigation of population dynamics using selective plating and a labeled pseudomonas putida CP1::Tn7-gfp showed domination of the latter. The new mixture performed a successful cooperation with good potential for FOG treatment and an aggregative response desirable to fat degradation in grease traps.

关键词： Bacillus Bioaugmentation Biodegradation FOG Lipase pseudomonas

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Selection of phages and conditions for the safe phage therapy against pseudomonas aeruginosa infections

引用

Virologica Sinica 2015年第1期30卷 33-44页

作者： Victor Krylov Olga Shaburova Elena Pleteneva Sergey Krylov Alla Kaplan Maria Burkaltseva Olga Polygach Elena Chesnokova I.I. Mechnikov Research Institute for Vaccines & Sera RAMS Moscow 105064 Russian

The emergence of multidrug-resistant bacterial pathogens forced us to consider the phage therapy as one of the possible alternative approaches to treatment. The purpose of this paper is to consider the conditions for the safe, long-term use of phage therapy against various infections caused by pseudomonas aeruginosa. We describe the selection of the most suitable phages, their most effective combinations and some approaches for the rapid recognition of phages unsuitable for use in therapy. The benefi ts and disadvantages of the various different approaches to the preparation of phage mixtures are considered, together with the specifi c conditions that are required for the safe application of phage therapy in general hospitals and the possibilities for the development of personalized phage therapy.

关键词： pseudomonas aeruginosa bacteriophages phage therapy pseudolysogeny phage-bacteria interactions phage-phage interactions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phosphorylation of the pseudomonas Effector AvrPtoB by Arabidopsis SnRK2.8 Is Required for Bacterial Virulence

引用

Molecular Plant 2020年第10期13卷 1513-1522页

作者： Lei Lei Danielle M.Stevens Gitta Coaker Department of Plant Pathology University of CaliforniaDavisDavisCAUSA

A critical component controlling bacterial virulence is the delivery of pathogen effectors into plant cells during infection.Effectors alter host metabolism and immunity for the benefit of pathogens.Multiple effectors are phosphorylated by host kinases,and this posttranslational modification is important for their activity.We sought to identify host kinases involved in effector phosphorylation.Multiple phosphorylated effector residues matched the proposed consensus motif for the plant calcium-dependent protein kinase(CDPK)and Snf1-related kinase(SnRK)superfamily.The conserved pseudomonas effector AvrPtoB acts as an E3 ubiquitin ligase and promotes bacterial virulence.In this study,we identified a member of the Arabidopsis SnRK family,SnRK2.8,which interacts with AvrPtoB in yeast and in planta.We showed that SnRK2.8 was required for AvrPtoB virulence functions,including facilitating bacterial colonization,suppression of callose deposition,and targeting the plant defense regulator NPR1 and analyses receptor FLS2.Mass spectrometry analysis revealed that AvrPtoB phosphorylation occurs at multiple serine residues in planta,with S258 phosphorylation significantly reduced in the snrk2.8 knockout.AvrPtoB phospho-'null'mutants exhibited compromised virulence functions and were unable to suppress NPR1 accumulation,FLS2 accumulation,or inhibit FLS2-BAK1 complex formation upon flagellin perception.Taken together,these data identify a conserved plant kinase utilized by a pathogen effector to promote disease.

关键词： Arabidopsis pseudomonas syringae effector AvrPtoB kinase SnRK2.8

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phospholipase C from pseudomonas aeruginosa and Bacillus cereus:characterization of catalytic activity

引用

Asian Pacific Journal of Tropical Medicine 2014年第11期7卷 860-866页

作者： Nooran Sherif Elleboudy Mohammad Mabrouk Aboulwafa Nadia Abdel-Haleem Hassouna Department of Microbiology and Immunology Faculty of PharmacyAin Shams University

Objective:To study characteristics of phospholipases C(PLCs),their importance for producing microorganisms us well us the potential of their use for industrial purposes.Methods:PLC from Bacillus cereus(B.cereus) D101 was selected as an example of Gram-positive PLCs and PLC from Pseudomanas aeruginosa(P.aeruginosa) D183 of Gram-negative ones.Enzymes were partially purified by ammonium sulfate precipitation followed by membrane dialysis.Partially purified preparations were used to study effect of different factors on activities as well as in substrate specificity tests which were conducted using a turbidimetric assay method.Results:Maximum activity was at pH 7 and 8 and 40 ℃ for P.aeruginosa PLC,and pH 8-10 and 37 ℃ for B.cereus PLC.Both PLCs were inhibited by Pi at 5 mM or higher,whereas,PLC from B.cereus only was inhibited by EDTA.Activity of P.aeruginosa PLC was not affected by removing Zn^(2+) ions from reaction mixture or their replacement with Ca^(2+),Ba^(2+),Mg^(2+) or Mn^(2+)ions.Vis-a-vis,activity of B.cereus PLC was found to be metal ion dependent PLCs from both isolates were relatively thermostable and showed maximum affinity toward phosphatidylcholine.Sphingomyelin and phosphatidylethanolamine were not good substrates and phosphatidylinositol,phosphatidylserine,phosphatidylglycerol and cardiolipin could be considered nonsubstrates.Conclusions:Human body physiological conditions could favor activity of P.aeruginosa and B.cereus PLCs.These enzymes may participate in phosphate scavenging and virulence of producing isolates but not in autolysis.PLCs from both isolates are potential candidates for industrial use.

关键词： Phospholipase C pseudomonas aeruginosa Bacillus cereus Catalytic activity Substrate specificity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Las-like quorum-sensing system negatively regulates both pyoluteorin and phenazine-1-carboxylic acid production in pseudomonas sp. M18

引用

Science China(Life Sciences) 2008年第2期51卷 174-181页

作者： CHEN Yun, WANG XiaoLei, HUANG XianQing, ZHANG XueHong & XU YuQuan Key Laboratory of Microbial Metabolism of Ministry of Education, College of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China Key Laboratory of Microbial Metabolism of Ministry of Education College of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai China

A las-like quorum-sensing system in pseudomonas sp. M18 was identified, which consisted of lasI and lasR genes encoding LuxI-LuxR type regulator. Several functions of the las system from strain M18 were investigated in this study. The chromosomal inactivation of either lasI or lasR by recombination increased the production of both pyoluteorin (Plt) and phenazine-1-carboxylic acid (PCA) by 4-5 fold and 2-3 fold over that of the wild type strain of M18, respectively. Production of both antibiotics was restored to wild-type levels after in trans complementation with the wild-type lasI or lasR gene. Expression of the translational fusions pltA'-'lacZ and phzA'-'lacZ further confirmed the negative effect of lasI or lasR on both biosynthetic operons, and it was also demonstrated that the las system was related to the ability of swarming motility and the inhibition of cell growth.

关键词： las system phenazine-1-carboxylic acid pyoluteorin swarming pseudomonas sp. M18

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Production of rhamnolipids-producing enzymes of pseudomonas in E. coli and structural characterization

引用

Frontiers of Chemical Science and Engineering 2017年第1期11卷 133-138页

作者： Kata Kiss Wei Ting Ng Qingxin Li Institute of Chemical & Engineering Sciences Agency for Science Technology and Research Jurong Island 627833 Singapore

Rhamnolipids are a class ofbiosurfactants that have a great potential to be used in industries. Five proteins/enzymes, namely RhlA, RhlB, RhlC, RhlG and RhlI, are critical for the production of rhamnolipids in pseudomonas aeruginosa. Four of the 5 proteins except RhlC were successfully over-expressed in E. coli and three of them （RhlA, RhlB and RhlI） were purified and obtained in milligram quantities. The purified proteins were shown to be folded in solution. Homology models were built for RhlA, RhlB and RhlI. These results lay a basis for further structural and functional characterization of these proteins in vitro to favor the construction of super strains for rhamnolipids production.

关键词： rhamnolipids pseudomonas RhlA RhlB,RhlI protein folding

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：