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The 12th Asian Pacific Confederation of Chemical Engineering Congress

＜正＞Industrial bakers’yeast strain Saccharomyces cerevisiae LH1 was selected for asymmetric re- duction of ethyl benzoylacetate to（S）-ethyl *** reductive effi- ciency and higher cofactor availability were obtaine... 详细信息

关键词： asymmetric bioreducfion Saccharomyces cerevisiae growth medium ethyl benzoylacetate proteome analysis

Phosphorus Starvation Tolerance in Rice Through Combined Physiological,Biochemical,and proteome Analyses

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Rice science 2023年第6期30卷 613-631页

作者： Prathap V Suresh KUMAR Nand Lal MEENA Chirag MAHESHWARI Monika DALAL Aruna TYAGI Division of Biochemistry Indian Council of Agricultural Research-Indian Agricultural Research InstituteNew Delhi 110012India Division of Germplasm Evaluation National Bureau of Plant Genetic ResourcesNew Delhi 110012India National Institute for Plant Biotechnology New Delhi 110012India

Phosphorus(P) deficiency limits the growth,development,and productivity of *** better understand the underlying mechanisms in P-deficiency tolerance and the role of Pup1 QTL in enhancing P use efficiency(PUE) for the development of P-efficient rice cultivars,a pair of contrasting rice genotypes(Pusa-44 and NIL-23) was applied to investigate the morpho-physio-biochemical and proteomic variation under P-starvation *** rice genotypes were grown hydroponically in a PusaRich medium with adequate P(16 mg/kg,+P) or without P(0 mg/kg,-P) for 30 d.P-starvation manifested a significant reductions in root and shoot biomass,shoot length,leaf area,total chlorophyll,and P,nitrogen and starch contents,as well as protein kinase *** stress increased root-to-shoot biomass ratio,root length,sucrose content,and acid phosphatase activity,particularly in the P-tolerant genotype(NIL-23).Comparative proteome analysis revealed several P metabolism-associated proteins(including OsCDPKs,OsMAPKs,OsCPKs,OsLecRK2,and OsSAPks) to be expressed in the shoot of NIL-23,indicating that multiple protein kinases were involved in P-starvation/deficiency ***,the up-regulated expression of OsrbcL,OsABCG32,OsSUS5,OsPoll-like B,and ClpC2 proteins in the shoot,and OsACA9,OsACA8,OsSPS2F,OsPP2C15,and OsBiP3 in the root of NIL-23,indicated their role in P-starvation stress control through the Pup1 QTL. Thus,our findings indicated that-P stress-responsive proteins,in conjunction with morpho-physio-biochemical modulations,improved PUE and made NIL-23 a P-deficiency tolerant genotype due to the introgression of the Pup1 QTL in the Pusa-44 background.

关键词： phosphorus deficiency Pup1 QTL protein kinase acid phosphatase proteome analysis rice

Comparative analyses of the venom components in the salivary gland transcriptomes and saliva proteomes of some heteropteran insects

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Insect Science 2022年第2期29卷 411-429页

作者： Kyungjae Andrew Yoon Woo-Jin Kim Seungki Lee Hee-Sun Yang Byoung-Hee Lee Si Hyeock Lee Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoulKorea EntoCode Co. SeoulKorea National Institute of Biological Resources Environmental Research ComplexIncheonKorea Department of Agricultural Biology Seoul National UniversitySeoulKorea

Salivary gland-specific transcriptomes of nine heteropteran insects with distinct feeding strategies (predaceous, hematophagous, and phytophagous) were analyzed and annotated to compare and identify the venom components as well as their expression profiles. The transcriptional abundance of venom genes was verified via quantitative real-time PCR. Hierarchical clustering of 30 representative differentially expressed venom genes from the nine heteropteran species revealed unique groups of salivary gland-specific genes depending on their feeding strategy. The commonly transcribed genes included a paralytic neurotoxin (arginine kinase), digestive enzymes (cathepsin and serine protease), an anti-inflammatory protein (cystatin), hexamerin, and an odorant binding protein. Both predaceous and hematophagous (bed bug) heteropteran species showed relatively higher transcription levels of genes encoding proteins involved in proteolysis and cytolysis, whereas phytophagous heteropterans exhibited little or no expression of these genes, but had a high expression of vitellogenin, a multifunctional allergen. Saliva proteomes from four representative species were also analyzed. All venom proteins identified via saliva proteome analysis were annotated using salivary gland transcriptome data. The proteomic expression profiles of venom proteins were in good agreement with the salivary gland-specific transcriptomic profiles. Our results indicate that profiling of the salivary gland transcriptome provides important information on the composition and evolutionary features of venoms depending on their feeding strategy.

关键词： hematophagous heteroptera phytophagous predaceous proteome analysis salivary gland-specific venom transcriptome analysis

FragAnchor: A Large-Scale Predictor of Glycosylphosphatidylinositol Anchors in Eukaryote Protein Sequences by Qualitative Scoring

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Genomics, Proteomics & Bioinformatics 2007年第2期5卷 121-130页

作者： Guylaine Poisson Cedric Chauve Xin Chen Anne Bergeron Department of Information and Computer Sciences University of Hawaii at Manoa Honolulu HI 96822 USA CGL ＆ Département d＇informatique Université du Québee à Montréal Montréal QC Canada LA CIM Université du QuEbec à MontrEal Montréal QC Canada Department of Mathematics Simon Fraser University Burnaby BC Canada.

A glycosylphosphatidylinositol （GPI） anchor is a common but complex C-terminal post-translational modification of extracellular proteins in eukaryotes. Here we investigate the problem of correctly annotating GPI-anchored proteins for the growing number of sequences in public databases. We developed a computational system, called FragAnchor, based on the tandem use of a neural network （NN） and a hidden Markov model （HMM）. Firstly, NN selects potential GPI-anchored proteins in a dataset, then HMM parses these potential GPI signals and refines the prediction by qualitative scoring. FragAnchor correctly predicted 91% of all the GPI-anchored proteins annotated in the Swiss-Prot database. In a large-scale analysis of 29 eukaryote proteomes, FragAnchor predicted that the percentage of highly probable GPI-anchored proteins is between 0.21% and 2.01%. The distinctive feature of FragAnchor, compared with other systems, is that it targets only the C-terminus of a protein, making it less sensitive to the background noise found in databases and possible incomplete protein sequences. Moreover, FragAnchor can be used to predict GPI-anchored proteins in all eukaryotes. Finally, by using qualitative scoring, the predictions combine both sensitivity and information content. The predictor is publicly available at http：//navet .***/- fraganchor/NNHMM/*** ml.

关键词： bioinformatics post-translational modification proteome analysis

S100A4 over-expression underlies lymph node metastasis and poor prognosis in colorectal cancer

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World Journal of Gastroenterology 2011年第1期17卷 69-78页

作者： Li-Yong Huang Department of Colorectal Surgery Fudan University Shanghai Cancer Center Shanghai 200032 China Department of Oncology Shanghai Medical College Fudan University Shanghai 200032 China Department of Pathology Fudan University Shanghai Cancer Center Shanghai 200032 China Department of Chemistry Fudan University Shanghai 200032 China Institute of Biomedical Sciences Fudan University Shanghai 200032 China

AIM:To develop lymph node metastasis(LNM)-associated biomarkers for colorectal cancer(CRC) using quantitative proteome ***:Differences in protein expression between primary CRC with LNM(LNM CRC) and without LNM(non-LNM CRC) were assessed using methyl esterification stable isotope labeling coupled with 2D liquid chromatography followed by tandem mass spectrometry(2D-LC-MS/MS).The relationship to clinicopathological parameters and prognosis of candidate biomarkers was examined using an independent sample ***:Forty-three proteins were found to be differentially expressed by at least 2.5-fold in two types of CRC.S100A4 was significantly upregulated in LNM CRC compared with non-LNM CRC,which was confirmed by Western blotting,immunohistochemistry and real-time quantitative polymerase chain *** immunohistochemistry on another 112 CRC cases showed that overexpression of S100A4 frequently existed in LNM CRC compared with non-LNM CRC(P < 0.001).Overexpression of S100A4 was significantly associated with LNM(P < 0.001),advanced TNM stage(P < 0.001),increased 5-year recurrence rate(P < 0.001) and decreased 5-year overall survival rate(P < 0.001).Univariate and multivariate analyses indicated that S100A4 expression was an independent prognostic factor for recurrence and survival of CRC patients(P < 0.05).CONCLUSION:S100A4 might serve as a powerful biomarker for LNM and a prognostic factor in CRC.

关键词： Colorectal cancer Lymph node metastasis Prognosis proteome analysis S100A4

Chromosome Science as the Science of the 21st Century

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Chromosome Science as the Science of the 21st Century

第一届亚洲植物染色体学术讨论会

作者： FUKUI Kiichi Department of Biotechnology Graduate School of EngineeringOsaka University Suita 565-0871 Japan

＜正＞ The direction of chromosome science in the 21st century is discussed based on the forefront data in the chromosome science already obtained. First, the higher organization of chromosomal structures becomes direct target of chromosome science. Rapid development of information technology and instrument enable to analyze all the proteins that are involved in construe-

关键词： Chromosome science proteome analysis Bioinformatics Nano- technology Visualization Genome and chromosome manipulation

苹果斑点落叶病菌与其宿主互作机制的蛋白质组学解析(英文)

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苹果斑点落叶病菌与其宿主互作机制的蛋白质组学解析(英文)

第三届全国植物蛋白质组学大会

作者：张彩霞丛佩华农业部果树种质资源利用重点开放实验室中国农业科学院果树研究所

Previous studies on the interaction between plants and pathogenic microorganisms were focused largely on some model plants and pathogens,while relatively few have been used in apple and its *** that apple alternaria blotch is the usual diseases influencing apple production,and that the previous studies were mostly around its pathogenic mechanism,further studies about the interaction mechanism between the pathogen and its host, particularly some host self-defense response mechanisms which closely related to the anti-disease properties of apple should be *** this project,the interaction system between Alternaria mali and its host will be involved to investigate the host defense mechanism induced by pathogen,which is expected to provide guidance for the future breeding study on new varieties of apple with the *** this aim,we will choose the high throughput proteomic approach,associated with the identification of mass spectrometry(MS),to investigate the interaction system between Alternaria mali and apple *** we hope to identify differentially expressed proteins related to resistance involved in the interaction system,and to aim at understanding the molecular mechanism of plant-pathogen *** project has many virtues to offer,such as providing clues to a comprehensive understanding of the molecular mechanism of apple resist Alternaria mali,accelerating the process of apple molecular breeding, and also providing some theoretical basis or technical reference for future genetic improvement study about high-quality disease-resistant apple varieties.

关键词： Alternaria mali Apple leaf Interaction mechanism proteome analysis

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应用蛋白质组学技术研究白血病细胞耐药机制

应用蛋白质组学技术研究白血病细胞耐药机制

中国有机质谱学第十三届全国学术大会

作者：仇晓燕齐静刘宁刘淑莹刘志强杨纯正中国科学院长春应用化学研究所新药研究实验室长春质谱中心中国医学科学院中国协和医科大学血液学研究所实验血液学国家重点实验室

蛋白质组学(Proteomics)是继基因组学之后兴起的前沿学科热点,是以基因组所表达的全部蛋白质,即蛋白质组(proteome)为研究对象的新的研究领域,其两大支柱是双向凝胶电泳技术和生物质谱技术。质谱技术是目前蛋白质组研究中发展最快,也最... 详细信息

蛋白质组学(Proteomics)是继基因组学之后兴起的前沿学科热点,是以基因组所表达的全部蛋白质,即蛋白质组(proteome)为研究对象的新的研究领域,其两大支柱是双向凝胶电泳技术和生物质谱技术。质谱技术是目前蛋白质组研究中发展最快,也最具活力和潜力的技术。诞生于八十年代末期基质辅助激光解吸电离质谱技术(Matrix Assisted Laser Desorption／Ionization,MALDI)使质谱技术进入了一个崭新的发展阶段,目前使用MALDI-TOF MS技术以得到酶解肽段的相对分子质量,获

关键词： Chemoresistance proteome analysis Two-dimensional electrophoresis MALDI-TOF MS