检索结果-南通市图书馆

Osmopriming-Regulated Changes of Plasma Membrane Composition and Function were Inhibited by Phenylarsine Oxide in Soybean Seeds

引用

Journal of Integrative Plant Biology 2009年第9期51卷 858-867页

作者： Jiajin Zhuo Weixiang Wang Yun Lu Wu Sen Xiaofeng Wang Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 China School of Bioengineering Xihua University Chengdu 610039 China

The aim of the present work was to investigate the effects of osmoconditioning on chilling injury in chilling-sensitive soybean （Glycine max （L.） Merr. Zhonghuang No. 22） seeds during imbibition. Low temperatures reduced the germination rate and no seed germinated at 1 ℃. Osmoconditioning of seeds at 20℃ with a polyethylene glycol-8000 （PEG8000） solution at 1.5 MPa for 72 h followed by drying back to their initial moisture content （MC） reduced their chilling sensitivity. The phenylarsine oxide （PAO）, an inhibitor of protein tyrosinephosphatases, was used to investigate the possible involvement of phosphorylation-dephosphorylation of tyr residues in the plasma membrane composition and function when seeds were osmoconditioned. The results showed the germination of osmoconditioned seeds decreased significantly when PAO was added in PEG solution after chilling treatment. PAO inhibited changes in composition of plasma membrane phospholipids and fatty acid induced by osmocondition, indicated that tyrosine protein phosphorylation is involved in the regulatory mechanisms of osmocondition-responsive chilling in soybean seeds. Western blot result further indicated that osmocondition treatment improved the activity of plasma membrane H^＋-ATPase after chilling treatment, but this effect was abolished by PAO. The possible regulation mechanism by tyr protein phosphorylation is discussed.

关键词： fatty acid membrane lipid osmoconditioning phenylarsine oxide protein-tyr phosphatases soybean seeds.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：