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PRELIMINARY STUDY OF EXTRACTABLE protein binding USING MALEIC ANHYDRIDE COPOLYMER

Chinese Journal of Polymer Science 2012年第2期30卷 181-189页

作者： Thirawan Nipithakul Ladawan Watthanachote Nanticha Kalapat College of Interdisciplinary Studies Thammasat University(Lampang)Lampang 52190Thailand Polymer Science and Technology Programme Faculty of ScienceMahidol UniversityBangkok 10400Thailand

A preliminary study of using maleic anhydride copolymer for protein binding has been carried out. The polymeric films were prepared by compression of the purified resin and annealing the film to induce efficient back formation of the anhydride groups. The properties of the film surface were analyzed by attenuated total reflection Fourier transforms infrared spectroscopy and water contact angle measurements. The protein content was determined by Bradford assay. To obtain optimum conditions, immersion time for protein binding was examined. Results revealed that proteins can be successfully immobilized onto the film surface via covalent linkage. The efficiency of the covalent binding of the extractable protein to maleic anhydride-polyethylene film was estimated at 69.87 ug/cm2, although the film had low anhydride content （3%） on the surface.

关键词： Maleic anhydride copolymer Extractable protein protein-binding Skim rubber.

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Organic coating of 1-2-nm-size silicon nanoparticles： Effect on particle properties

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Nano Research 2015年第6期8卷 2047-2062页

作者： Cristian R. Lillo Juan J. Romero Manuel Llansola Portoles Reinaldo Pis Diez Paula Caregnato Monica C. Gonzalez Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas (INIFTA) CCT-La Plata-CONICET Facultad de Ciencias Universidad National de La Plata cc 16 Suc. 4 1900 La Plata Argentina CEQUINOR CCT-La Plata-CONICET Facultad de Ciencias Exactas Universidad Nacional de La Plata cc 9621900 La Plata Argentina Department of Chemistry and Biochemistry Center for Bioenergy and Photosynthesis Arizona State University Tempe Arizona USA

Photoluminescent silicon nanoparticles 1-2 nm in size were synthesized by a wet chemical procedure and derivatized with propylamine （NH2SiNP）. Surface NH2 groups were used as linkers for additional poly（ethylene glycol）（PEG） and folic acid （Fo） attachment （PEG-NHSiNP and Fo-NHSiNP, respectively） to enable efficient targeting of the particles to tumors and inflammatory sites. The particles were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, ζ potential, dynamic light scattering, and time-resolved anisotropy. The photophysical properties and photosensitizing capacity of the particles and their interaction with proteins was dependent on the nature of the attached molecules. While PEG attachment did not alter the photophysical behavior of NH2SiNP, the attachment of Fo diminished particle photoluminescence. Particles retained the capacity for 1O2 generation; however, efficient 1O2 quenching by the attached surface groups may be a drawback when using these particles as 1O2photosensitizers. In addition, Fo attachment provided particles with the capacity to generate the superoxide anion radical （O2-）. The particles were able to bind tryptophan residues of bovine serum albumin （BSA） within quenching distances. NH2SiNP and PEG-NHSLNP ground state complexes with BSA showed binding constants of （3.1 ± 0.3） × 10^4 and （1.3 ±0.4） × 10^3 M-1, respectively. The lower value observed for PEG-NHSiNP complexes indicates that surface PEGylation leads to a reduction in protein adsorption, which is required to prevent opsonization. An increase in particle luminescence upon BSA binding was attributed to the hydrophobic environment generated by the protein. NH2SiNP-BSA complexes were also capable of resonance energy transfer.

关键词： propylamine folic acid poly(ethylene glycol) photoluminescence protein-binding singlet oxygen

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