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Relationship with spatial memory in diabetic rats and protein kinase cγ,caveolin-1 in the hippocampus and neuroprotective effect of catalpol

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chinese Medical Journal 2014年第5期127卷 916-923页

作者： Zhou Haicheng Liu Jing Ren Liyuan Liu Wei Xing Qian Men Lili Song Guirong Du Jianling Department of Endocrinology First Affiliated Hospital of Dalian Medical University Dalian Liaoning 116011 China

Background The mechanisms underlying diabetic encephalopathy are largely unknown,and no effective treatments are *** has received much attention due to its numerous biological effects,especially in neuroprotective *** aim of this study was to investigate the effects of catalpol on cognitive functions in diabetic rats and the underlying *** A rat model of diabetes was established by streptozotocin injection,followed by intraperitoneal infusion of catalpol after 10 *** weeks later,the Morris water maze was used to test the spatial learning *** staining was performed to evaluate the morphological changes in the *** of protein kinase cy （PKcy） and caveolin-1 （cav-1） in the hippocampus were assessed by reverse transcription PcR and Western *** of anti-oxidative enzymes such as glutathione （GSH）,superoxide dismutase （SOD） and catalase （cAT） and levels of malonaldehyde （MDA） were measured using commercial *** Significant hippocampal neuronal injury was observed in rats with streptozotocin-induced ***,cognitive dysfunction was associated with markedly increased oxidative stress in the *** treatment significantly attenuated cognitive deficits,neuronal damage,and oxidative stress in the brain of diabetic *** analyses showed that catalpol reversed the down-regulation of PKcγ and cav-1 expression in the diabetic *** Spatial memory in diabetic rats is associated with the expression of PKcy and *** treatment markedly attenuated oxidative stress,reversed the alteration of PKcy,cav-1 and spatial memory deficits.

关键词： catalpol diabetic encephalopathy protein kinase cγ caveolin-1

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Essential role of protein kinase c in morphine-induced rewarding memory

Essential role of protein kinase C in morphine-induced rewar...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Xing-Jie PING~#,Yao-Ying MA~#,Yi-Jing LI,chong QI,Xiao-Wei SUN,Xiu-Fang LV,cai-Lian cUI~* Neuroscience Research Institute,Department of Neurobiology,School of Basic Medical Sciences,Key Laboratory of Neuroscience,The Ministry of Education and the Ministry of Public Health,Peking University,Beijing 100191,china

＜正＞Objective protein kinase c（PKc） is involved in intra-cellular signal transduction in various physiological and pathological processes including substance *** the present study,the role of PKc in morphine-induced rewarding memory was investigated using the conditioned place preference（cPP） *** cPP was induced by different doses of morphine（0-10 mg/kg,i.p.）.To observe the effect of PKc on the development of cPP （induced by 3 mg/kg morphine,i.p.）,microinjection cannulas were bilaterally implanted in NAc shell and PKc translocation inhibitor yV5-3 was infused before the conditioning of morphine-induced ***（1） We found a significant translocation of PKcs from cytosol to membrane component in nucleus accumbens（NAc） of morphine-conditioned rats in a dose-dependent *** translocation was reduced gradually with the maintenance of morphine-induced ***,the protein level of PKcy in membrane of the NAc was increased in morphine cPP rats,and decreased during the attenuation of morphine-induced cPP,while the protein level of PKcy in cytosol of the NAc showed an opposite change.（2） The PKc translocation inhibitor yV5-3 impaired the morphine-induced cPP when microinjected into the *** These findings indicated that PKc,especially the y isoform,is essential for the acquisition and maintenance of morphine associated reward memory.

关键词： nucleus accumbens morphine conditioned place preference protein kinase c protein kinase cγ γV5-3

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大鼠皮质脊髓束的应用解剖学研究

大鼠皮质脊髓束的应用解剖学研究

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作者：刘苏南通大学

学位级别：硕士

第一部分皮质脊髓束在大鼠脊髓内的精确定位目的：确定大鼠皮质脊髓束在延髓和脊髓白质内的走行和精确定位。方法：选用成年SD大鼠，采用Luxol fast blue(LFB、)染色法和protein kinase cγ(PKcγ)特异性抗体免疫组织化学染色法在冠状... 详细信息

第一部分皮质脊髓束在大鼠脊髓内的精确定位目的：确定大鼠皮质脊髓束在延髓和脊髓白质内的走行和精确定位。方法：选用成年SD大鼠，采用Luxol fast blue(LFB、)染色法和protein kinase cγ(PKcγ)特异性抗体免疫组织化学染色法在冠状面、矢状面、横断面三种不同切面上对正常大鼠皮质脊髓束进行解剖定位。结果：在延髓锥体中，LFB染色标记的深蓝色的有髓纤维经锥体交叉至脊髓颈、胸、腰、骶段后索腹侧，与周围淡染的纤维易于区别;PKcγ阳性反应产物呈棕色，分布于延髓腹侧面的锥体中，在延髓锥体下部绝大部分交叉到背侧面，在脊髓白质内沿后索腹侧下行，至脊髓的骶尾段。同时延髓和脊髓背角神经元中也有PKcγ免疫阳性物质存在，在脊髓前索和外侧索未见有PKcγ阳性反应产物。LFB阳性纤维和PKcγ免疫阳性反应产物在延髓锥体和脊髓后索内的定位分布与大鼠皮质脊髓束在延髓和脊髓中的位置和走行相一致。结论：运用LFB染色和PKcγ特异性抗体免疫组织化学染色可以清晰对正常大鼠皮质脊髓束进行精确解剖定位，为进一步研究皮质脊髓束的损伤和功能重建提供形态学基础。第二部分大鼠皮质脊髓束半横段损伤模型的建立目的：成功制作大鼠皮质脊髓束半横断损伤模型。方法：选择性切割大鼠延髓左侧锥体，建立大鼠皮质脊髓束半横断损伤模型，采用斜板实验进行行为学检测，运用LFB染色法、生物素化葡聚糖胺(BDA)神经示踪法和PKcγ免疫组织化学染色法对模型进行形态学评判。结果：皮质脊髓束损伤组大鼠术后右侧前后肢瘫痪，斜板实验显示运动功能受限;LFB染色可以看出损伤部位左侧皮质脊髓束无Luxol Fast Blue浓染神经纤维束;BDA神经示踪法结果显示在锥体交叉平面，仅见一束BDA阳性反应产物经右侧交叉至左侧，在脊髓后索的左侧半下行至骶尾段，而在损伤平面以下，未见有BDA阳性反应产物经左侧锥体交叉至右侧，在整个脊髓后索的右侧半未见有BDA阳性反应产物。PKcγ免疫组织化学染色结果发现延髓锥体右侧半有一束PKcγ阳性反应产物经右侧交叉至左侧，脊髓后索腹侧部左侧半有PKcγ阳性反应产物，而脊髓后索腹侧部右侧半未见有PKcγ阳性反应产物。结论：选择在延髓切断一侧锥体制作皮质脊髓束半横断损伤模型，定位准确，重复性好，结果可靠，是研究皮质脊髓束的可塑性和神经轴突再生的理想动物模型。BDA神经束路示踪法和PKcγ免疫组织化学染色法可作为皮质脊髓束半横断损伤模型的形态学指标。第三部分大鼠皮质脊髓束半横断损伤后皮质脊髓束超微结构的变化目的：观察皮质脊髓束半横断损伤后皮质脊髓束超微结构的变化。方法：选择性切割大鼠延髓左侧锥体，建立皮质脊髓束半横断损伤模型，模型制作后4d、14d、28d，采用透射电镜观察受损皮质脊髓束超微结构的变化。结果：皮质脊髓束半横断损伤后，受损皮质脊髓束髓鞘和轴索发生肿胀，形态不规则。随着时间的延长，溃变进行性加重，受损皮质脊髓束主要表现为髓鞘破坏、溶解及轴索脱髓鞘病变、胞浆浓缩、细胞器增多及空泡样变性。脊髓颈、胸、腰段受损皮质脊髓束的溃变比损伤部位严重。结论：皮质脊髓束半横断损伤后，受损皮质脊髓束中的髓鞘和轴索发生进行性溃变。

关键词：皮质脊髓束 protein kinase cγ 免疫组织化学 Luxol fast blue 皮质脊髓束半横断损伤生物素化葡聚糖胺 PKcγ 皮质脊髓束超微结构半横断损伤溃变髓鞘

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蛋白激酶c在吗啡诱导奖赏记忆中的关键作用(英文)

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中国药物依赖性杂志 2010年第5期19卷 424-425页

作者： PING Xingjie,MA Yaoying,LI Yijing,QI chong,cUI cailian(Neuroscience Research Institute,Peking University Department of Neurobiology,School of Basic Medical Sciences,Peking University Key Laboratory of Neuroscience,The Ministry of Education and Ministry of Public Health,38 Xueyuan Road,Beijing,100191,PR china)

protein kinase c (PKc) is involved in intra-cellular signal transduction in various physiological and pathological processes including substance abuse. In the present study,the role of PKc in morphine-induced rewarding memory was investigated using the conditioned place preference (cPP) model. We found a significant translocation of PKcs from cytosol to membrane component in nucleus accumbens (NAc) of morphine-conditioned rats in a dose-dependent manner. The translocation was reduced gradually with the spontaneous extinction of morphine-induced cPP. Specifically,the protein level of PKcγ in membrane of the NAc was increased in morphine cPP rats,and decreased during the attenuation of morphine-induced cPP,while the protein level of PKcγ in cytosol of the NAc showed an opposite change. Furthermore,the PKc translocation inhibitor γV5-3 impaired the morphine induced cPP when microinjected into the NAc. These findings indicated that PKc,especially the γ isoform,is essential for the acquisition and maintenance of morphine associated reward memory.

关键词： nucleus accumbens morphine conditioned place preference protein kinase c protein kinase cγ γV5-3

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