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RNA-binding domain of the Key Structural protein P7 for the Rice dwarf virus Particle Assembly

Acta Biochimica et Biophysica Sinica 2005年第1期37卷 55-60页

作者： Bo-Xiong ZHONG~(1,2*) Yan-Wei SHEN~1 Toshihiro OMURA~2 ~1 Department of Bioresource Science.College of Animal Science,Zhejiang University,Hangzhou 310029,China ~2National Agricultural Research Center,Tsukuba.Ibaraki 305,Japan Department of Bioresource Science College of Animal Science Zhejiang UniversityHangzhou 310029 China National Agricultural Research CenterTsukuba Ibaraki 305 Japan

The Rice dwarf virus(RDV)P7 structural protein is the key protein in the RDV *** P7 protein was digested partially or completely by Staphylococcus aureus V8 protease and/orPseudomonas fragi Asp-N *** molecular mass and the N-terminal amino acid sequence of thepolypeptide fragments of the P7 protein were determined by SDS-PAGE and the Edman degradation method,*** the polypeptides were located in the deduced amino acid sequence of the RDV P7 proteinbased on the nucleotide sequence information,with the knowledge of the specific cleavage sites of theStaphylococcus aureus V8 and Pseudomonas fragi Asp-N protease,and the two RNA-binding domains in theP7 protein were *** 1 was located in the residue 128-249 containing 122 amino acids anddomain 2 was located in the residue 325-355 containing 31 amino ***,these two domains may playan important role in the virus particle assembly by contributing to the packaging of viral dsRNAs inside *** two domains may be novel RNA-binding domains,because no amino acid sequences highlysimilar to the conservative sequences of known dsRNA-binding domains reported so *** similaritybetween the motif of domain 1 and the motif of the DNA-binding protein suggests that the DNA-bindingactivity of the RDV P7 protein may be due to this *** similarity between the motif of domain 1 andthe motif of the RNA polymerase domain suggests that the P7 protein may also play a role in RNA synthesis,besides its function in the assembly and subsequent packaging of viral dsRNA into core particles.

关键词： RDV RNA-binding protein protein functional domain virion assembly RNA polymerase

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