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Electrochemical aptasensor for the detection of vascular endothelial growth factor(VEGF) based on DNA-templated Ag/Pt bimetallic nanoclusters

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Chinese Chemical Letters 2016年第6期27卷 920-926页

作者： Xian-Ming Fu Zhi-Jing Liu Shu-Xian Cai Yan-Ping Zhao Dong-Zhi Wu Chun-Yan Li Jing-Hua Chen College of Pharmacy and Medical Technology Putian University Department of Pharmaceutical Analysis the Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian ProvinceThe School of Pharmacy Fujian Medical University Department of Basic Chemistry The School of Pharmacy Fujian Medical University

In this paper, the DNA-templated Ag/Pt bimetallic nanoclusters were successfully synthesized using an optimized synthetic scheme. The obtained DNA-Ag/Pt NCs have an ultrasmall particle size and excellent distribution. The DNA-Ag/Pt NCs show intrinsic peroxidase-mimicking activity and can effectively catalyze the H2O2-mediated oxidation of a substrate, 3,30,5,50-tetramethylbenzidine（TMB）, to produce a blue colored product. Based on this specific property, we employed the aptamer of VEGF to design a label-free electrochemical biosensor for VEGF detection. Under the optimized experimental conditions, a linear range from 6.0 pmol/L to 20 pmol/L was obtained with a detection limit of 4.6 pmol/L. The proposed biosensor demonstrated its high specificity for VEGF and could directly detect the VEGF concentration in human serum samples of breast cancer patients with satisfactory results. This novel electrochemical aptasensor was simple and convenient to use and was cost-effective and label-free in design, and would hold potential applications in medical diagnosis and treatment.

关键词： Vascular endothelial growth factor(VEGF) DNA-templated Ag/Pt bimetallic nanoclusters Enzyme mimics Aptamer Electrochemical aptasensor protein detection

Enzyme-catalyzed deposition of polydopamine for amplifying the signal inhibition to a novel Prussian blue-nanocomposite and ultrasensitive electrochemical immunosensing

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Journal of Materials Science & Technology 2022年第7期102卷 166-173页

作者： Wan Huang Peng Guo Bo Li Li Fu Cheng-Te Lin Aimin Yu Guosong Lai Hubei Key Laboratory of Pollutant Analysis&Reuse Technology College of Chemistry and Chemical EngineeringHubei Normal UniversityHuangshi 435002China College of Materials and Environmental Engineering Hangzhou Dianzi UniversityHangzhou 310018China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Department of Chemistry and Biotechnology Faculty of ScienceEngineering and TechnologySwinburne University of TechnologyHawthornVIC 3122Australia

The uncontrollable synthesis of Prussian blue (PB) and its weak stability toward OH -are great challenges affecting its electrochemical biosensing application. Herein we utilize the unique properties of chitosan (CS) to realize the facile and controllable synthesis of a CS-PB nanocomposite and combine it with the urease-catalyzed deposition of polydopamine (PDA) for amplifying the electrochemical signal inhibition of PB to develop a novel immunosensing method for protein detection. The immunosensor was constructed on a CS-PB modified electrode, and a urease-functionalized silica nanoprobe was prepared for tracing its sandwich immunoassay toward the model analyte of carcinoembryonic antigen. Besides the electrochem- ical impedance effect of the quantitatively captured nanoprobes, their enzymatic reaction can release numerous OH -to destroy the PB crystals and also induce the PDA deposition onto the immunosensor. These caused drastic electrochemical signal inhibition to PB. Based on the above multi-signal amplification mechanism, the method exhibits a very low detection limit of 0.042 pg mL^(-1) along with a very wide linear range of six-order of magnitude. In addition, the CS-PB based immunosensor has excellent specificity, repeatability, stability and reliability. Thus this PB nanocomposite and the proposed electrochemical immunosensing method reveal a promising potential for future applications.

关键词： Prussian blue Polydopamine Biosensor Electroanalytical chemistry protein detection

Crosstalk-free colloidosomes for high throughput single-molecule protein analysis

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Science China Chemistry 2020年第10期63卷 1507-1514页

作者： Kun Yin Xi Zeng Xuan Liang Haopai Wei Huimin Zeng Wei Qi Weidong Ruan Yanling Song Chaoyong Yang Zhi Zhu The Key Laboratory of Chemical Biology of Fujian Province the MOE Key L aboratory of Spectrochemical Analysis&InstrumentationState Key Laboratory of Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Chemical EngineeringDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China

Droplet microfluidics is a powerful platform for high-throughput single-molecule protein ***,the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide *** address these limitations,a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein *** the self-assembly of easily synthesized colloidal surfactant F-SiO2 NPs at the water/oil interface,the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent *** crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis,including heterogenous dynamic studies and digital *** a robust and accurate method,colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications,such as directed enzyme evolution,digital enzyme-linked immunosorbent assay(ELISA),and screening of antibiotics.

关键词： colloidosomes droplet microfluidics crosstalk-free single-molecule analysis protein detection

Highly Sensitive protein Sensor Based on Thermally-Reduced Graphene Oxide Field-Effect Transistor

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Nano Research 2011年第10期4卷 921-930页

作者： Shun Mao Kehan Yu Ganhua Lu Junhong Chen Department of Mechanical Engineering University of Wisconsin-Milwaukee 3200 N. Craraer St. Milwaukee WI 53211 USA

We report the fabrication of a highly sensitive field-effect transistor （FET） biosensor using thermally-reduced graphene oxide （TRGO） sheets functionalized with gold nanoparticle （NP）-antibody conjugates. Probe antibody was labeled on the surface of TRGO sheets through Au NPs and electrical detection of protein binding （Immunoglobulin G/IgG and anti-lmmunoglobulin G/anti-lgG） was accomplished by FET and direct current （dc） measurements. The protein binding events induced significant changes in the resistance of the TRGO sheet, which is referred to as the sensor response. The dependence of the sensor response on the TRGO base resistance in the sensor and the antibody areal density on the TRGO sheet was systematically studied, from which a correlation of the sensor response with sensor parameters was found： the sensor response was more significant with larger TRGO base resistance and higher antibody areal density. The detection limit of the novel biosensor was around the 0.2 ng/rnL level, which is among the best of,＇eported carbon nanomaterial-based protein sensors and can be further optimized by tuning the sensor structure.

关键词： Biosensor thermally-reduced graphene oxide field-effect transistor protein detection

Comparison between Two Methods for Detecting Gene Flow and Analysis on the Influencing Factors

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Agricultural Science & Technology 2011年第6期12卷 840-841,861页

作者：贺娟朱家林刘小侠张青文中国农业大学农学与生物技术学院北京100193

[Objective] The aim was to test the consistency between two commonly used methods PCR detection and protein detection for detecting gene flow,and investigate the factors influencing the consistency.[Method] 1 769 samples of three varieties under three treatments（wind,bee and control）were detected with both methods.[Result] There was phenomenon in field that Bt gene was transferred into F1 generations but couldn＇t express,that meant,the result of PCR detection was not consistent with that of protein *** comparing environmental factors,it was proved that wind and bee treatments didn＇t significantly affect the expression of gene ***,the Bt gene expression rate in bee treatment was higher than that in wind treatment.[Conclusion] The paper will provide reference for accurate detection of gene flow.

关键词： Gene flow PCR protein detection