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Revealing the Structure-Property Difference of Natural Rubber Prepared by Different Methods: protein and Gel Content are Key Factors

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Chinese Journal of Polymer Science 2024年第4期42卷 457-467页

作者： Shao-Qi Huang Jun-Qi Zhang Yong Zhu Ling-Min Kong Lu-Sheng Liao Fu-Quan Zhang Zheng-Tian Xie Jin-Rong Wu State Key Laboratory of Polymer Materials Engineering College of Polymer Science and EngineeringSichuan UniversityChengdu610065China Hainan Provincial Key Laboratory of Natural Rubber Processing Agricultural Products Processing Research Institute of Chinese Academy of Tropical Agricultural SciencesZhanjiang524001China

Natural rubber (NR) is widely used in various fields including aerospace, military industry and transportation due to its superior elasticity and comprehensive mechanical properties. Nonetheless, the commercial NR prepared by different methods usually exhibits different mechanical properties, primarily due to variations in processing conditions during the conversion from latex to bulk rubber material. Consequently, this poses challenges in scientific research and industrial production of NR. In order to assess the properties of various commercially available NR and identify key structural and compositional components, this study systematically compares and analyzes four representative NR raw materials: air dried sheet (ADS), ribbed smoked sheets (RSS), constant viscidity NR (CV), and whole field latex rubber (WF). The investigation focuses on evaluating their static mechanical behavior, SIC behavior, wear resistance, and fatigue resistance. The findings indicate that protein and gel content exhibit a crucial influence on the NR properties. These constituents contribute to the formation of a high-crosslinking density region, generating a heterogeneous network structure within the rubber. This structure amplifies strains during deformation, leading to earlier and stronger strain-induced crystallization (SIC). Among the four commercial NR brands, RSS demonstrates superior overall mechanical and dynamic properties owing to its high protein and gel content. This study serves as a valuable reference for comprehending the differences in properties among various commercial NR, thereby offering guidance for the actual processing and selection of NR.

关键词： Natural rubber Processingmethod protein Gel content Property

Barley protein LFBEP-C1 from Lactiplantibacillus plantarum dy-1 Fermented Barley Extracts by Inhibiting Lipid Accumulation in a Caenorhabditis elegans Model

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Biomedical and Environmental Sciences 2024年第4期37卷 377-386页

作者： ZHANG Jia Yan LIU Meng Ting LIU Yu Hao DENG Huan BAI Juan XIE Jian Hua XIAO Xiang School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina State Key Laboratory of Food Science and Technology Nanchang UniversityNanchang 330047JiangxiChina

Objective This study aimed to investigate the lipid-lowering activity of LFBEP-C1 in high glucose-fed Caenorhabditis elegans(***).Methods In this study,the fermented barley protein LFBEP-C1 was prepared and tested for its potential anti-obesity effects on *** worms were fed Escherichia coli OP50(*** OP50),glucose,and different concentrations of *** size,lifespan,movement,triglyceride content,and gene expression were *** results were analyzed using ANOVA and Tukey's multiple comparison *** Compared with the model group,the head-swing frequency of *** in the group of LFBEP-C1 at 20μg/mL increased by 33.88%,and the body-bending frequency increased by 27.09%.This indicated that LFBEP-C1 improved the locomotive ability of *** average lifespan of *** reached 13.55 days,and the body length and width of the *** decreased after LFBEP-C1 ***,LFBEP-C1 reduced the content of lipid accumulation and triglyceride *** expression levels of sbp-1,daf-2,and mdt-15 significantly decreased,while those of daf-16,tph-1,mod-1,and ser-4 significantly increased after LFBEP-C1 *** in these genes explain the signaling pathways that regulate lipid *** LFBEP-C1 significantly reduced lipid deposition in *** fed a high-glucose diet and alleviated the adverse effects of a high-glucose diet on the development,lifespan,and exercise behavior of *** addition,LFBEP-C1 regulated lipid metabolism mainly by mediating the expression of genes in the sterol regulatory element-binding protein,insulin,and 5-hydroxytryptamine signaling pathways.

关键词： LFBEP-C1 Fermentation protein Caenorhabditis elegans Lipid accumulation Signaling pathway

Effect of operational parameters on the performance of an anaerobic sequencing batch reactor(AnSBR)treating protein-rich wastewater

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Environmental Science and Ecotechnology 2024年第1期17卷 89-99页

作者： Zhe Deng Julian Muñoz Sierra Ana Lucia Morgado Ferreira Daniel Cerqueda-Garcia Henri Spanjers Jules B.van Lier Delft University of Technology Faculty of Civil Engineering and GeosciencesStevinweg 12628 CNDelftthe Netherlands Veolia Water Technologies Techno Center Netherlands B.V.-Biothane Tanthofdreef 212623 EWDelftthe Netherlands KWR Water Research Institute Groningenhaven 7P.O.Box 10723430 BBNieuwegeinthe Netherlands Institute of Ecology.A.C Cluster Cientifico y Tecnologico BioMimic®Carretera Antigua a Coatepec 351El Haya91073XalapaVeracruzMexico

Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several *** this study,we applied an anaerobic sequencing batch reactor(AnSBR)to treat protein-rich wastewater from a *** focused on identifying the key factors influencing the removal of chemical oxygen demand(COD)and the settling performance of the *** AnSBR achieved a maximum total COD removal of 90%,a protein degradation efficiency exceeding 80%,and a COD to methane conversion efficiency of over 70%at organic loading rates of up to 6.2 g COD L^(-1)d^(-1).We found that the variations in both the organic loading rate within the reactor and the hydraulic retention time in the buffer tank had a significant effect on COD *** hydraulic retention time in the buffer tank and the reactor,which determined the ammonification efficiencies and the residual carbohydrate concentrations in the reactor liquid,affected the sludge ***,the genus Clostridium sensu stricto 1,known as protein-and lipids-degraders,was predominant in the *** analysis showed a significant correlation between the core microbiome and ammonification efficiency,highlighting the importance of protein degradation as the governing process in the *** results will provide valuable insights to optimise the design and operation of AnSBR for efficient treatment of protein-rich wastewater.

关键词： Anaerobic digestion Anaerobic sequencing batch reactor (AnSBR) protein Microbial community Slaughterhouse wastewater

Molecular pathology and protein markers for pancreatic cancer:relevance in staging,in adjuvant therapy,in determination of minimal residual disease,and follow-up

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Hepatobiliary Surgery and Nutrition 2024年第1期13卷 56-70页

作者： Caitlin P.Sok Karunesh Polireddy David A.Kooby Department of Surgery Emory University School of MedicineAtlantaGAUSA

The diagnosis and monitoring of disease through the detection of circulating protein biomarkers is a growing field in the practice of *** search for more effective protein biomarkers to aid in the diagnosis and treatment of patients with pancreatic ductal adenocarcinoma(PDAC)remains a valuable area of study,given the aggressive and often occult nature of this *** biopsies are attractive,as they offer a minimally invasive and cost-effective approach when compared to traditional biopsy methods and imaging modalities used for diagnosis and *** antigen(CA)19-9 is currently the most commonly used serum protein biomarker for the diagnosis and monitoring of patients with PDAC,but due to its sensitivity and specificity,its utility remains *** this review,we examine how circulating protein biomarkers are used in the diagnosis,prognostication,and surveillance of *** also highlight protein biomarkers that are currently under investigation that have the potential to enhance our ability to detect early-stage malignancies,predict response to therapy,and monitor for recurrence,but these markers require larger prospective validation studies before they can be widely *** efforts to identify and validate novel biomarkers will be crucial for improving the management and outcomes of patients with this challenging disease.

关键词： Biomarkers pancreatic adenocarcinoma protein

A Review on Characteristics, Extraction Methods and Applications of Renewable Insect protein

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Journal of Renewable Materials 2024年第5期12卷 923-950页

作者： Adelya Khayrova Sergey Lopatin Valery Varlamov Research Centre of Biotechnology of the Russian Academy of Sciences Moscow119071Russia

Due to the expected rise in the world population,an increase in the requirements for quality and safety of food and feed is expected,which leads to the growing demand for new sources of sustainable and renewable *** protein is gaining importance as a renewable material for several reasons,reﬂecting its potential contribu-tions to sustainability,resource efﬁciency,and environmental *** insect species are known to be able to efﬁciently convert organic waste into high-value products such as protein,requiring less land and water compared to traditional *** addition,insect farming produces fewer greenhouse gas emissions,contri-buting to mitigating climate *** are considered as a major potential alternative to animal or plant protein due to their many nutritional beneﬁts,including high protein,mineral,and vitamin *** average,the protein content of insects ranges between 35%and 60%dry weight,which exceeds plant protein sources,such as cereal,soybeans,and *** the acceptance of insect protein grows and technologies advance,the food and feed industries continue to explore and expand their applications,offering consumers diverse and sustainable pro-tein *** this review,we discuss the recentﬁndings relating to insect protein focusing on its characteristics,extraction methods,applications,and opportunities along with some trade-offs and uncertainties.

关键词： Circular economy waste utilisation protein insect Hermetia illucens

Near Infrared Spectroscopy (NIRS) Model-Based Prediction for protein Content in Cowpea

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American Journal of Plant Sciences 2024年第3期15卷 145-160页

作者： Kavera Biradar Waltram Ravelombola Aurora Manley Caroline Ruhl Texas A&M AgriLife Research Vernon TX USA University of Agricultural Sciences Dharwad India Department of Soil and Crop Sciences Texas A&M University College Station TX USA

Cowpea (Vigna unguiculata L. Walp) is a multi-purpose legume with high quality protein for human consumption and livestock. The objective of this work was to develop near-infrared spectroscopy (NIRS) prediction models to estimate protein content in cowpea. A total of 116 cowpea breeding lines with a wide range of protein contents (19.28 % to 32.04%) were selected to build the model using whole seed and ground seed samples. Partial least-squares discriminant analysis (PLS-DA) regression technique with different pre-treatments (derivatives, standard normal variate, and multiplicative scatter correction) were carried out to develop the protein prediction model. Results showed: 1) spectral plots of both the whole seed and ground seed showed higher spectral scatter at higher wavelengths (>1450 nm), 2) data pre-processing affects prediction accuracy for bot whole seed and ground seed samples, 3) prediction using ground seed samples (0.64 R2 0.85) is better than the whole seed (0.33 R2 0.78), and 4) the data pre-processing second derivative with standard normal variate has the best prediction (R2_whole seed = 0.78, R2_ground seed = 0.85). The results will be of interest in cowpea breeding programs aimed at improving total seed protein content.

关键词： Cowpea Germplasm protein Near-Infrared Spectroscopy (NIRS) Partial Least Squares (PLS)

protein-derived nitrogen and sulfur co-doped carbon for efficient adsorptive removal of heavy metals

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Chinese Journal of Chemical Engineering 2019年第10期27卷 2581-2586页

作者： Yawei Shi Wei Zheng Hao Liu Liang Wang Hongwei Zhang State Key Laboratory of Separation Membranes and Membrane Processes Tianjin Polytechnic UniversityTianjin 300387China School of Environmental and Chemical Engineering Tianjin Polytechnic UniversityTianjin 300387China

A nitrogen and sulfur co-doped carbon has been synthesized employing egg white as a sustainable protein-rich *** to CHNS elemental analysis,N,S and O heteroatoms accounted for mass fractions of 3.66%,2.28%and 19.29%respectively,and the types of surface functionalities were further characterized by FT-IR and XPS *** the carbon possessed a smaller surface area(815 m2·g-1)compared to a commercial activated carbon(1100 m2·g-1),its adsorption capacity towards Co2+reached 320.3 mg·g-1,which was over 8 times higher compared to the limited 34.0 mg·g-1 over the activate ***,the carbon was found to be an efficient adsorbent towards a series of metal ions including VO2+,Cr3+,Ni2+,Cu2+and Cd2+.Combined with its environmental merits,the protein derived carbon may be a promising candidate for heavy metal pollution control.

关键词： protein Porous carbon Heteroatoms Adsorption Heavy metal Waste water

protein conformation and electric attraction adsorption mechanisms on anodized magnesium alloy by molecular dynamics simulations

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Journal of Magnesium and Alloys 2022年第11期10卷 3143-3155页

作者： Zhao-Qi Zhang Hong-Yan Wang Li Wang Xiao-Bo Chen Shao-Kang Guan Cun-Guo Lin Rong-Chang Zeng Corrosion Laboratory for Light Metals College of Material Science and EngineeringShandong University of Science and TechnologyQingdao 266590China School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450002China State Key Laboratory for Marine Corrosion and Protection Luoyang Ship Material Research InstituteQingdao 266101China School of Engineering RMIT UniversityCarlton VIC 3053Australia

protein adsorption preferentially occurs and significantly affects the physicochemical reactions once the biodegradable magnesium alloys as bone replacements have been implanted. To date, interactions mechanisms between Mg implants and proteins remain unclear at a molecular level. Thereby, a combination of molecular dynamic(MD) simulations and experimental exploration is used to investigate the adsorption behavior and conformational change of bovine serum albumin(BSA), a representative protein of blood plasma, upon the surface of microarc oxidation(MAO) coated Mg alloy AZ31. The influences of absorbed proteins on the cytocompatibility of MAO coating are evaluated by virtue of cytotoxicity assay. Results indicate that the negatively charged O atoms(BSA) exhibit strong interaction with Mg^(2+) ions of Mg(OH)_(2), revealing that BSA molecules are ionically adsorbed on the AZ31 surface. Interestingly, MD simulation reveals that MAO coating demonstrates superior ability to capture BSA molecules during the process of adsorption owing to strong electric attraction between the negatively charged O atoms in BSA molecules with Mg atoms of MgO in MAO coating. Moreover, the α-helix part of absorbed BSA molecules on AZ31 substrate and MAO coating markedly decreases with an increase in β-sheet, β-turn and unordered contents, which is attributed to the reduction in the number of hydrogen bonds in BSA molecules. Furthermore, the adsorbed BSA molecules improve the cytocompatibility of MAO coating since the positively charged-NH_(3)^(+) group and β-sheet content of absorbed BSA molecules mediate the cell adhesion by interacting with the negatively charged cell membrane.

关键词： Magnesium alloy Molecular dynamics simulations protein Conformation Biomaterial

protein-sheathed SWNT as a versatile scaffold for nanoparticle assembly and superstructured nanowires

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Science China Chemistry 2018年第9期61卷 1128-1133页

作者： Jinjing Wu Yang Yang Zhaoxiang Deng Department of Chemistry University of Science and Technology of China

One dimensional(1 D) nanostructures have many possible applications in electronic, optical, and sensing devices associated with their nanosized lateral dimensions. In this regard, a general and bottom-up strategy to synthesize 1 D nanoparticle arrays and conductive nanowires with a facile structural/compositional control is highly desired. We herein report a protein-sheathed single walled carbon nanotube(SWNT) that satisfies the criteria for an ideal template to assemble micron-long gold nanoparticle(Au NP) linear arrays with high structural rigidity. The resulting Au NP array has minimized inter-particle gaps, which is especially useful to template the overgrowth of Ag, Pd, and Pd/Ag metals into continuous nanowires(Au@M, M=Ag, Pd, Ag/Pd). Our method successfully overcomes the incompatibility between carbon and metal materials, and the resulting superstructured metal nanowires have a tunable diameter below 100 nm and a shape closely replicating a SWNT. The Ag nanowires are composed of coalesced Au@Ag coreshell nanoparticles, while the Pd and Pd/Ag nanowires are made of very fine Pd nanocrystallites around the Au NP cores. These unique structural features are pivotal to various applications including surface enhanced Raman scattering(SERS), electrocatalysis, and gas sensors.

关键词： SWNT protein nanoparticle self-assembly nanowire