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The Effect of Curcumin on Proliferation and Apoptosis in LNCaP Prostate Cancer Cells

Chinese Journal of Clinical Oncology 2006年第1期3卷 55-60页

作者： Lei Yang Lijun Chen Bin Meng Jiangrui Suo Hongmin Wang Hong Xie Qiuyue Jin Li Yao Ruimin Wang Lianying Zhang Department of Biochemistry Medical College of the Chinese Armed Police Forces Tianjin 300162 China Deparment of Hygiene Medical College of the Chinese Armed Police Forces Tianjin 300162 China Institute of Biochemistry Shandong University Shandong 250100 China

OBJECTIVE To observe the effect of curcumin on proliferation and apop-tosis in the prostate cancer LNCaP cell line. METHODS The AXSYMTM system luciferase method was used to examine the effect of various concentratious of curcumin on the content of prostate specific antigen (PSA) in prostate cancer LNCaP cells. A pGL3-PSA luciferase expression vector, containing 640 bp DNA of the PSA gene 5' -promoter region was constructed and transfected into the LNCaP cells with lipofectin. By measuring luciferase activity, the effect of 10 μmol/L, 20 μmol/L, 30 and 40 μmol/L curcumin on the promoter was studied. Effects on cell growth and apoptosis were analyzed by microscopy, the MTT colorimetric assay and flow cytometry. Western-blotting was used to measure expression of the androgen receptor (AR) in the LNCaP cells treated with different concentrations of curcumin. RESULTS The results showed that the expression of PSA was inhibited as curcumin reduced the activity of luciferase. Curcumin also caused a sigificant concentration-dependent decrease in AR expession measured by Western -blotting. Cell growth was inhibited and apoptosis was induced. CONCLUSION By inhibiting AR expression, curcumin reduced the function of the PSA promoter and inhibited PSA protein expression. Curcumin decreased the cellular proliferation and induced apoptosis in LNCaP cells in a concention-dependent manner.

关键词： curcumin androgen receptor prostrate specific antigen apoptosis LNCaP cells.

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