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From pro-prostate specific antigen, [-2]pro-prostate specific antigen to Beckman Coulter phi： the evolution of new biomarkers for early detection of prostatic carcinoma

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Chinese Medical Journal 2012年第9期125卷 1643-1649页

作者： ZHANG Ming-zhi LU Yi-ping Department of Urology West China HospitalSichuan UniversityChengduSichuan 610041China

prostate specific antigen （PSA） has a wide clinical use for the early detection of prostatic carcinoma （PCa）; however, it has never been a perfect marker due to its low specificity and low positive predictive value which ranges between 4 ng/ml and 10 ng/ml. The discovery of different PSA molecular forms in serum in the early 1990s brought insight into searching for more specific markers. Since then free PSA （fPSA） has been used routinely to increase the specificity for PCa and to reduce unnecessary biopsies. More recently, promising data is emerging regarding one proenzyme molecular form of free PSA, proPSA, and a few truncated proPSA isoforms. The purpose of this article is to review the recent studies on clinical utility of proPSA, especially [-2]pPSA, an isoform of proPSA, and parameters involving [-2]pPSA as well as other PSA derivatives in early detection of PCa.

关键词： prostate cancer prostate specific antigen enzyme precursors tumor markers

Contemporary outcomes in the detection of prostate cancer using transrectal ultrasound-guided 12-core biopsy in Singaporean men with elevated prostate specific antigen and/or abnormal digital rectal examination

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Asian Journal of Urology 2015年第4期2卷 187-193页

作者： Alvin Lee Sing Joo Chia Yong Loo Lin School of Medicine National University of SingaporeSingapore Department of Urology Tan Tock Seng HospitalSingapore

Objective:Despite being the third commonest cancer in Singaporean men,there is a dearth of basic data on the detection rate of prostate cancer and post-procedure complication rates locally using systematic 12-core biopsy.Our objective is to evaluate prostate cancer detection rates using 12-core prostate biopsy based on serum prostate specific antigen(PSA)levels and digital rectal examination(DRE)findings in Singaporean men presenting to a single tertiary centre.The secondary objective is to evaluate the complication rates of transrectal prostate biopsies.Methods:We retrospectively examined 804 men who underwent first transrectal-ultrasound(TRUS)guided 12-core prostate biopsies from January 2012 to April 2014.prostate biopsies were performed on men presenting to a tertiary institution when their PSA levels were4.0 ng/mL and/or when they had suspicious DRE findings.Results:Overall prostate cancer detection rate was 35.1%.Regardless of DRE findings,patients were divided into four subgroups based on their serum PSA levels:0e3.99 ng/mL,4.00 e9.99 ng/mL,10.00e19.99 ng/mL and20.00 ng/mL and their detection rates were 9.5%,20.9%,38.4% and 72.3%,respectively.The detection rate of cancer based on suspicious DRE findings alone was 59.2% compared to 36.5% based on serum PSA cut-off of 4.0 ng/mL alone.The post-biopsy admission rate for sepsis was 1.5%.Conclusion:In conclusion,using contemporary 12-core biopsy methods,the local prostate cancer detection rate based on serum PSA and DRE findings has increased over the past decade presumably due to multiple genetic and environmental factors.Post-biopsy sepsis remains an important complication worldwide.

关键词： Digital rectal examination Oncology prostate cancer prostate specific antigen Transrectalultrasound Biopsy

Mass screening of prostate cancer in a Chinese population:the relationship between pathological features of prostate cancer and serum prostate specific antigen

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Asian Journal of Andrology 2005年第2期7卷 159-163页

作者： Hong-WenGao Yu-LinLi ShanWu Yi-ShuWang Hai-FengZhang Yu-ZhuoPan LingZhang HirooTateno IkuroSato MasaakiKuwahara Xue-JianZhao DepartmentofPathology JilinUniversitySecondHospitalChangchun130041China／／ProstateDiseasesPreventionandTreatmentResearchCenterJilinUniversityChangchun130021China DepartmentofPathology BasicMedicalCollegeJilinUniversityChangchun130021China prostateDiseasesPreventionandTreatmentResearchCenter JilinUniversityChangchun130021China DepartmentofPathology MiyagiCancerCenterMiyagiPrefectureJapan DepartmentofUrology MiyagiCancerCenterMiyagiPrefectureJapan

Aim:To investigate the pathological features of the prostate biopsy through mass screening for prostate cancer in a Chinese cohort and their association with serum prostate specific antigen (PSA).Methods:A total of 12 027 Chinese men in Changchun were screened for prostate cancer by means of the serum total prostate specific antigen (tPSA) test (by Elisa assay).Transrectal ultrasound-guided systematic six-sextant biopsies were performed on those whose serum tPSA value was >4.0 ng/mL and those who had obstructive symptoms (despite their tPSA value) and were subject to subse- quent pathological analysis with the aid of the statistic software SPSS 10.0 (SPSS.Inc.,Chicago.USA).Results:Of the 12 027 cases,158 (including 137 patients whose serum tPSA values were >4.0 ng/mL and 21 patients [serum tPSA prostate biopsy.Of the 158 biopsies,41 cases of prostatic carci- noma were found (25.9 %,41/158).The moderately differentiated carcinoma and poorly differentiated carcinoma ac- counted for 61% and 34 %,respectively.A significant linear positive correlation between the serum tPSA and the Gleason scores in the 41 cases of prostatic carcinoma (r=0.312,Pprostate cancer by testing for serum tPSA values and the first to investigate the pathological features of prostate cancer in a cohort of Chinese men.Our results reveal that the moderately differentiated carcinoma is the most common type of prostate cancer.This study also has shown that the serum tPSA value in prostate cancer is associated with the Gleason score and the size of tumor.

关键词： prostate cancer mass screening prostate specific antigen pathomorphology

Ultrasensitive electrochemical sensor for prostate specific antigen detection with a phosphorene platform and magnetic covalent organic framework signal amplifier

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Ultrasensitive electrochemical sensor for prostate specific ...

Construction of Smac gene-containing and human prostate specific antigen promoter-regulated vector and its expression

第十一届全国化学生物学学术会议

作者： Huan Liang Hanbin Xu Yuting Zhao Jing Zheng Hui Zhao Guoliang Li Can-Peng Li School of Chemical Science and Technology·College of Pharmacy Yunnan University

Magnetic covalent organic frameworks(COFs) are useful mesoporous materials for the enrichment and separation of analytes,and are utilized.Herein,a novel electrochemical assay for the determination of prostate specific antigen(PSA) was developed using black phosphorene(BPene) as a platform and magnetic COFs for signal amplification.BPene was prepared via water-phase exfoliation.BPene nanocomposite(Au@BPene) was prepared by depositing Au nanoparticles(Au NPs) onto BPene.This nanocomposite was utilized as an in the pretreatment of samplesimmunsensing platform to bind primary antibodies and improve electron transfer.Subsequently,an Au NP-loaded magnetic COF was used to immobilize the secondary antibodies and abundant electronic signals of methylene blue(MB).The effective signal amplification of the proposed sensor is attributed to the good electron transfer of Au@BPene,excellent enrichment capacity of signal molecules(MB) of the COF,and efficient catalytic activity of FeO.This work not only provides an effective electrochemical assay to detect PSA in real sample,but also broadens the utilization scope of magnetic COFs in immunosensing.

关键词： Electrochemical immunosensor magnetic covalent organic framework phosphorene prostate specific antigen

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Journal of Nanjing Medical University 2007年第3期21卷 134-138页

作者： Yu Wu Fuqing Zeng Liang Wang Yanbo Wang Guiyi Liao Department of Urology Union Hospital Tongji Medical College Huazhong University of Science and Technotogy Wuhan 430022 China

To construct an eukaryotic expression vector containing Smac gene and study the expression efficiency and specificity of prostate specific antigen（PSA） enhancer/promoter in a possible targeted gene therapy scheme for prostate cancer. Methods： PSA enhancer （PSAE） and promoter （PSAP） sequences were amplified using PCR method. CMV and T7 promoters were deleted from pcDNA3.1-Smac and replaced by the two specific fragments to generate pPSAE-PSAP-Smac. After transfection into different cell lines, the status of cells was observed. And then, we determined the relative concentration of Smac mRNA in RT-PCR. Results： The recombinant plasmid of pPSAE-PSAP-Smac was successfully constructed. And only the prostate cancer cell line PC-3 was suppressed after transfection with pPSAE-PSAP-Smac. However, other nonprostate lines were not. Moreover, the concentration of Smac mRNA regulated by PSA promoter and enhancer was higher in comparison to the CMV promoter-driven control vectors. Conclusion： An expression vector containing the Smac gene （based on elements of the PSA gene regulatory sequences） has been developed and shown to function in prostate cancer cell lines which provides a solid platform for launching clinical studies.

关键词： prostate specific antigen enhancer promoter Smac gene therapy

A retrospective study:Correlation of histological inflammation in biopsy specimens of men underwent surgery for benign prostatic hyperplasia with serum prostate specific antigen

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A retrospective study:Correlation of histological inflammati...

Combined Detection of Serum Heat Shock Protein-90<i>α</i>and prostate specific antigen for prostate Cancer Diagnosis

第十五届全国泌尿外科学术会议

作者：魏强宋灵敏四川大学华西医院泌尿外科

Purpose: We attempted to find out the correlation between BPH histological inflammation and serum PSA concentrations, and to reveal the possible mechanism. Patients and methods: Patients underwent surgery at the Urology Department of West China Hospital of Sichuan University from January 2005 to July 2007 were retrospectively studied.

关键词： prostate benign prostatic hyperplasia prostate specific antigen histological inflammation theory of leakage

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Yangtze Medicine 2020年第4期4卷 284-292页

作者： Shichang Zhang Jiang Zheng Yangtze University Jingzhou China Jingzhou First People’s Hospital Jingzhou China

Objective: To explore the relationship between heat shock protein-90α (HSP-90α) and occurrence of prostate cancer, and clinical value of combined detection of serum HSP-90α and prostate specific antigen (PSA) in the diagnosis of prostate cancer. Method: A total of 30 patients with prostate cancer, 30 patients with benign prostatic hyperplasia (BPH) and 30 healthy men (control group) were selected from September 2018 to September 2019, then to detect levels of serum HSP-90α, total PSA and free PSA (FPSA) by ELISA, serum testosterone level by radioimmunoassay, prostate cancer tissue was removed by operation, and relative expression of tissue HSP-90α protein by Western blot. Results: The levels of serum HSP-90α and total PSA in prostate cancer group were significantly higher than other two groups, and testosterone level was lower than other two groups (P 0.05). It was found by Pearson test that serum HSP-90α was positively correlated with total PSA level (r = 0.659, P = 0.005), while negatively correlated with testosterone level (r = -0.549, P = 0.006). According to TNM stage of prostate cancer, there were 17 cases of stage I - II, 13 cases of stage III - IV, 6 cases of Gleason score 1 - 4, 13 cases of 5 - 7, 11 cases of 8 - 10, tumor diameter range from 0.8 to 6.2 cm, with average of (3.9 ± 1.5) cm. The relative expression of HSP-90α protein in tumor tissue was closely related to TNM stage, Gleason score and tumor diameter (P α and PSA levels for prostate cancer diagnosis was 0.896, and that of single PSA detection was 0.852. Conclusion: Higher expressions of HSP-90α in prostate cancer tissue and serum may be closely related to occurrence and development of prostate cancer, and combined detections of serum HSP-

关键词： Heat Shock Protein-90α prostate specific antigen prostate Cancer

EVALUATION OF FREE-TO-TOTAL prostate specific antigen RATIO IN THE DIAGNOSIS OF prostate CANCER

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Chinese Journal of Cancer Research 2000年第2期12卷 114-114页

作者：邵远张祖豹 DepartmentofUrology RuijinHospitalShanghaiSecondMedicalUniversity

It''s reported that free to total prostate specific antigen ration (f/tPSA) can provide more benefit than the single use of prostate specific antigen (PSA) in the diagnosis of prostate cancer (PCa). We measured serum PSA and fPSA levels in 62 cases of benign prostatic hyperplasia (BPH) and 40 cases of PCa using radioimmunoassay, with patients'' age range 59y~ 89y.

关键词： prostate Cancer prostate specific antigen Benign Prostatic Hyperplasia Serum prostate specific antigen Total prostate specific antigen

Early diagnosis of prostate cancer using free/total prostate specific antigen ratio: a population based screening data

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Asian Journal of Andrology 2004年第4期6卷 342-342页

作者： LingZhang Guo-YIJi Xiao-MengLi Wei-HuaWang Hong-WenGao Yu-ZhuoPan Hong-JunWang KuwaharaMasaaki Xue-JianZhao ResearchCenterforprostateDiseasePreventionandTreatment JilinUniversityChangchun130021China ResearchInstituteofMilitaryMedicine ShenyangCommandShenyang110000China DivisionofUrology MiyagiCancerCenterNatori981Japan

Aim: To evaluate the use of free/total prostate specific antigen ratio (fPSA/tPSA ratio) in improving the early diagnosis of prostate cancer. Methods: The fPSA/tPSA ratio in the serum was analyzed in 187 men with tPSA ranging between 4.0 and 20.0 μg/L. All of them underwent ultrasound guided sextant prostatic biopsy. The results were calculated by SPSS 10.0 software. Results: (1) When the tPSA was within the ranges of 4.0 - 10.0 and 10.0 -20.0 μg/L, the prostate cancer detection rate was 18.1 % and 22.5 %, respectively; (2) The area under the curve (AUC) was bigger in fPSA/tPSA than in tPSA (Pprostate cancer detection rate and reduce unnecessary biopsies when tPSA is within the range of 4.0 - 20.0 μg/L.

关键词： prostate cancer prostate specific antigen free/total PSA ratio