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PRGL:A cell wall proline-rich protein containning GASA domain in Gerbera hybrida

Science China(Life Sciences) 2008年第6期51卷 520-525页

作者： PENG JianZong, LAI LiuJing & WANG XiaoJing College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China College of Life Sciences South China Normal University Guangdong Provincial Key Lab of Biotechnology for Plant Development Guangzhou China

PRPs (proline-rich proteins) are a group of cell wall proteins characterized by their proline and hy- droproline-rich repetitive peptides. The expression of PRPs in plants is stimulated by wounding and environmental stress. GASA (gibberellic acid stimulated in Arabidopsis) proteins are small peptides sharing a 60 amino acid conserved C-terminal domain containing twelve invariant cysteine residues. Most of GASAs reported are localized to apoplasm or cell wall and their expression was regulated by gibberellins (GAs). It has been reported that, in French bean, these two proteins encoding by two distinct genes formed a two-component chitin-receptor involved in plant-pathogen interactions when plant was infected. We cloned a full-length cDNA of PRGL (proline-rich GASA-like) gene which encodes a protein containing both PRP and GASA-like domains. It is demonstrated that PRGL is a new protein with characteristics of PRP and GASA by analyzing its protein structure and gene expression.

关键词： cell wall protein proline-rich proteins (PRPs) gibberellic acid stimulated in Arabidopsis (GASA) proline-rich GASA-like (PRGL) Gerbera hybrida

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On the Distribution of Acidic proline-rich Proteins Phenotypes in Chinese Han Population in Liaoning Area

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中国医科大学学报 1991年第S2期20卷 33-36页

作者：庞灏贾静涛胡忠国 Department of Forensic Serology Faculty of Forensic Medicine China Medical University Shenyang China

The distribution of salivary acidic proline-rich proteins pheno-types was investigated by using the ultra-thin PAGIEF technique in 258 ChineseHan populations in Liaoning area. The gene frequencies were as follows: Pr^10.8101, Pr^20.1899; Db^+0.0416, Db^-0.9584; Pa^+0.1717, Pa^-0.8283; PIF^+0.6647, PIF^-0.3353. The observed numbers of the phenotypes are in good a-greement with the expected numbers under the Hardy-Weinberg *** gene frequencies among the Chinese and other populations are compared.

关键词： acidic proline-rich proteins polyacrylamide gel isoelectric focusing gene frequency

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Increased Electrophoretic Mobility of Long-Type GATA-6 Transcription Factor upon Substitution of Its PEST Sequence

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Advances in Bioscience and Biotechnology 2014年第13期5卷 1032-1042页

作者： Kanako Obayashi Kayoko Takada Kazuaki Ohashi Ayako Ohashi-Kobayashi Mayumi Nakanishi-Matsui Makoto Araki Masatomo Maeda Laboratory of Biochemistry and Molecular Biology Graduate School of Pharmaceutical Sciences Osaka University Suita Japan Department of Medical Biochemistry School of Pharmacy Iwate Medical University Shiwa-Gun Japan Department of Immunobiology School of Pharmacy Iwate Medical University Shiwa-Gun Japan Department of Biochemistry School of Pharmacy Iwate Medical University Shiwa-Gun Japan Department of Molecular Biology School of Pharmacy Iwate Medical University Shiwa-Gun Japan

The transcriptional factor GATA-6 gene produces two translational isoforms from a single mRNA through ribosomal leaky scanning. L-type GATA-6 has an extension of 146 amino acid residues at its amino terminus. In the extension, there is a unique PEST sequence (Glu31-Cys46), which is composed of an amino terminal Pro-rich segment and a carboxyl terminal Ser-cluster. Substitution of either half of the PEST sequence with Ala residues by cassette mutagenesis reduced the apparent molecular size of L-type GATA-6 on SDS-polyacrylamide gel-electrophoresis. However, the effect of substitution of the Pro-rich segment was much more significant;the mobility increase of the Pro-rich segment on the gel was 13% while that of the Ser-cluster was 8%. Substitution of each amino acid residue demonstrated that the effect of Pro substitution is greater than that of the Ser and Thr residues. Such increased mobility of L-type GATA-6 in the presence of a detergent may apparently correlate with the decrease in transcription activity in vivo as determined by means of luciferase reporter gene assay. The activity of ΔAla (with Ala residues instead of the PEST sequence) was reduced to one fifth of that of ΔA (with the PEST sequence). These results suggest that the PEST sequence of L-type GATA-6 does not function as a constitutive protein degradation signal, but rather plays structural and functional roles in the activation of gene expression on the GATA responsive promoter.

关键词： Cassette Mutagenesis Long-Type GATA-6 Mobility on Gel-Electrophoresis PEST Sequence proline-rich Segment Transcription Factor

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Viral proteins and Src family kinases: Mechanisms of pathogenicity from a “liaison dangereuse”

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World Journal of Virology 2013年第2期2卷 71-78页

作者： Mario Angelo Pagano Elena Tibaldi Giorgio Palù Anna Maria Brunati Department of Molecular Medicine University of Padua

To complete their life cycle and spread, viruses interfere with and gain control of diverse cellular processes, this most often occurring through interaction between viral proteins(VPs) and resident protein partners. Among the latter, Src family kinases(SFKs), a class of non-receptor tyrosine kinases that contributes to the conversion of extracellular signals into intracellular signaling cascades and is involved in virtually all cellular processes, have recently emerged as critical mediators between the cell's infrastructure and the viral demands. In this scenario, structural or ex novo synthesized VPs are able to bind to the different domains of these enzymes through specific short linear motifs present along their sequences. proline-rich motifs displaying the conserved minimal consensus PxxP and recognizing the SFK Src homology(SH)3 domain constitute a cardinal signature for the formation of multiprotein complexes and this interaction may promote phosphorylation of VPs by SFKs, thus creating phosphotyrosine motifs that become a docking site for the SH2 domains of SFKs or other SH2 domain-bearing signaling molecules. Importantly, the formation of these assemblies also results in a change in the activity and/or location of SFKs, and these events are critical in perturbing key signalingpathways so that viruses can utilize the cell's machinery to their own benefit. In the light of these observations, although VPs as such, especially those with enzyme activity, are still regarded as valuable targets for therapeutic strategies, multiprotein complexes composed of viral and host cell proteins are increasingly becoming objects of investigation with a view to deeply characterize the structural aspects that favor their formation and to develop new compounds able to contrast viral diseases in an alternative manner.

关键词： Interaction Phosphotyrosine proline-rich motif Src homology 2 domain Src homology 3 domain

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