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Studies on the physiological function of spermine in the process of progesterone induced toad oocyte maturation

Cell Research 1992年第2期2卷 103-117页

作者： JlAKE TSO Shanghai Institute of Cell Biology Academia Sinica

Spermidine or spermine but not putrescine inhibitedprogesterone induced Bufo bufo gargarizans oocyte *** IDfor spermine inhibition via intra-oocyte mi-croinjection on maturation.induced by progesterone was 6.8mM(100 nl).Spermine could inhibit MPF induced toadoocyte maturation.with a much higher ID. A 55 kD protein was dephosphorylated during the pro-cess of progesterone induced oocyte *** promoted the level of phosphorylation of thisprotein in both progesterone-stimulated and hormone-un-treated *** extent of its dephosphorylation wasfairly correlated with the percentage of GVBD in the hor-mone stimulated oocytes. The level of endogenous spermine was reduced by 28%between the period of 0.40 GVBDand 0.60 GVBD,atwhich 55 kD protein was dephosphorylated. Spermine inhibited progesterone-stimulated proteinsynthesis in almost the same dose dependent manner as itsinhibitory effect on the hormone-induced *** spermine regulated 55 kD protein dephosphory-lation which may trigger the increase of protein synthesisand in turn promote the activation of *** is possiblethat 55 kD protein may be one of the components of messen-ger ribonucleoprotein(mRNP)particles.

关键词： spermine toad oocyte progesterone induced maturation.

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Casein kinase G may be the target of spermine during progesterone-induced oocyte maturation

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Cell Research 1993年第1期3卷 61-74页

作者： LI RUNSHENG AND JIAKE TSOShanghai Institute of Cell Biology, Academia Sinica Shanghai Institute of Cell Biology Academia Sinica

Casein kinase G (CKG) with more than 2500-fold enrichment was purified from Bufo bufo gargarizans *** catalytic activity of the enzyme was found to be associated with its 42 kD subunit, and its 26 kD subunit was found to be the major target for the enzyme auto phosphorylation. Each full-grown oocyte contained 1.9 units of CKG corresponding to an intracellular concentration of 93 nM. After injecting an amount of 0.38 units of the enzyme into the oocyte, approximately 50％ of the progesterone-induced maturation.was inhibited. The inhibitory effect was enhanced in oocytes pretreated with spermine, which was consistent with the results that the enzyme was activated in vitro in the presence of spermine. The MPF-induced oocyte maturation.was delayed and even prohibited in the kinase-microinjected oocytes.A 55 kD oocyte protein was identified as an substrate of CKG both in vivo and in vitro, and the enhancement of the 55 kD protein phosphorylation was associated with kinase inhibition on maturation.and on protein synthesis in kinase-microinjected oocytes. As the endogenous spermine level decreased in the course of progesterone induced oocyte maturation. 55 kD protein was dephospho rylated. Heparin, a specific inhibitor of CKG, potentiated the progesterone-induced oocyte maturation. Altogether the experimental results indicated strongly that CKG may be the physiological target of spermine.

关键词： casein kinase G spermine toad oocyte progesterone induced maturation.

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