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Methodology of factorial design deriving guidelines for simulation of growth curve and production of sugars by Spirulina (Arthrospira) maxima

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Journal of Central South University of Technology 2001年第4期8卷 228-233页

作者： NIE Zhen yuan XIA Jin lan LEVERT J.M. Institute of Bioengineering Department of Mineral EngineeringCentral South UniversityChangsha 410083China Service de Chimie et Biochimie Appliquées FacultéPolytechnique de MonsMons 7000Belgique

It is practical, economic and sometimes essential to derive rules or conclusions by performing lesser runs of experiments. In this part, a methodology based on 2 f factorial design was brought up to derive guidelines to simulate growth curve and production of sugars by Spirulina (Arthrospira) maxima . The growth curve or accumulation process of sugars was idealized by sets of straight lines limited by phase transfers of growth or accumulation of sugars. Normal analyses of the critical values of the transfers were used to derive their linear relationships with the initial conditions of the experimental factors. These linear functions were called guidelines and were used to simulate the growth curve or accumulation of sugars. Generalization of the guideline technique was determined by the kinetic limitation of nutrient nitrogen or sulfur that was dependent upon their stoichiometric deficiency directly derived from their initial values in the medium. This method uses the initial conditions of culture and does not need measurements of concentrations of nitrate, sulfate and pigments during cultivation. It is a practical and useful alternative way to trace and predict approximately the growth curve and production of sugars by S. maxima .

关键词： factorial design guideline simulation growth production of sugar Arthrospira Spirulina maxima

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