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An Integration Strategy to Develop Dual-State Luminophores with Tunable Spectra,Large Stokes Shift,and Activatable Fluorescence for High-Contrast Imaging

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CCS Chemistry 2022年第6期4卷 2153-2164页

作者： Yongchao Liu Lili Teng Chengyan Xu Tian-Bing Ren Shuai Xu Xiaofeng Lou Lin Yuan Xiao-Bing Zhang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringCollaborative Innovation Center for Chemistry and Molecular MedicineHunan UniversityChangsha 410082

Developing dual-state luminophores(DSLs)with strong fluorescence in both the monomer and aggregate states is urgently needed but remains a huge challenge because most current luminophores are either aggregation-induced emission or aggregation-caused quenching ***,limited by the structural conservation of the few existing DSLs,there are not enough response sites that can be used to customize various activatable fluorescent probes for specific molecular ***,we engineered a general integration strategy for the fabrication of such DSLs with excellent photophysical *** DSLs,with their tunable spectra,a large Stokes shift(>170 nm),and achievable near-infrared(NIR)emission,show great potential for high-contrast ***,DSLs can be used as a universal platform for probe customization due to their activatable fluorescence through protection-deprotection of the phenolic hydroxyl *** on this,an NIR fluorescent probe DSL-Gal was developed for sensing of β-galactosidase in solutions,senescent cells,and liver metastases with high contrast,further confirming the superiority and universal feasibility of DSLs in probe *** integration strategy may provide a novel approach for the generation of other DSLs and have great potential applications in bioimaging.

关键词： dual-state luminophores probe customization probe platform enzyme activity high-contrast imaging

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