检索结果-南通市图书馆

Effects of polydimethylsiloxane membrane surface treatments on human uterine smooth muscle cell strain response

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2024年第2期32卷 415-426页

作者： Elizabeth E.Marr Brett C.Isenberg Joyce Y.Wong Boston University Division of Materials Science and EngineeringUnited States Charles Stark Draper laboratory Bioengineering DivisionUnited States Boston University Department of Biomedical EngineeringUnited States

In the United States,1 in 10 infants are born *** majority of neonatal deaths and nearly a third of infant deaths are linked to preterm *** birth is initiated when the quiescent state of the uterus ends prematurely,leading to contractions and parturition beginning as early as 32 weeks,though the origins are not well *** enable research and discovery of therapeutics with potential to better address preterm birth,the capability to study isolated cell processes of pregnant uterine tissue in vitro is *** development of an in vitro model of the myometrium utilizing human uterine smooth muscle cells(uSMCs)responsible for contractions provides a methodology to examine cellular mechanisms of late-stage pregnancy potentially involved in preterm *** discuss culture of uSMCs on a flexible polydimethylsiloxane(PDMS)substrate functionalized with cationic poly-L-lysine(PLL),followed by extracellular matrix(ECM)protein *** work exploring uSMC behavior on PDMS substrates have utilized collagen-I coatings,however,we demonstrated the first exploration of human uSMC response to strain on fibronectin-coated flexible membranes,importantly reflecting the significant increase of fibronectin content found in the myometrial ECM during late-stage *** the model we developed,we conducted proof-of-concept studies to investigate the impact of substrate strain on uSMC cell morphology and gene *** was found that PLL and varied ECM protein coatings(collagen I,collagen III,and fibronectin)altered cell nuclei morphology and density on PDMS ***,varied strain rates applied to uSMC substrates significantly impacted uSMC gene expression of IL-6,a cytokine associated with instances of preterm *** results suggest that both surface and mechanical properties of in vitro systems impact primary human uSMC phenotype and offer uSMC culture methodologies that can be utilized to further the understanding of cellu

关键词： Uterine Myometrium Bioengineered in vitro models Strain Pregnancy Nuclei Interleukin 6 preterm labor

An imbalance between innate and adaptive immune cells at the maternal-fetal interface occurs prior to endotoxin-induced preterm birth

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2016年第4期13卷 462-473页

作者： Marcia Arenas-Hernandez Roberto Romero Derek St Louis Sonia S Hassan Emily B Kaye Nardhy Gomez-Lopez Department of Obstetrics & Gynecology Wayne State University School of Medicine Detroit Michigan USA perinatology Research Branch Program for Perinatal Research and Obstetrics Division of Intramural Research Eunice Kennedy ShriverNational Institute of Child l~ealth and Human DevelopmentNIC.HD/NIHDHHS Bethesda Maryland and Detroit Michigan USA Departme.nt of Obstetrics and Gynecology University of M chigan Ann ArborMichigan USA Department of Epidemiology and Biostatistics Michigan State University East Lansing Michigan USA Department of Molecular Obstetrics and Genetics Wayne State University Detroit Michigan USA Department of ImmunoLogy & MicrobioLogy Wayne State University School of Medicine Detroit Michigan USA

preterm birth （PTB） is the leading cause of neonatal morbidity and mortality worldwide. A transition from an anti-inflammatory state to a pro-inflammatory state in the mother and at the maternal-fetal interface has been implicated in the pathophysiology of microbial-induced preterm labor. However, it is unclear which immune cells mediate this transition. We hypothesized that an imbalance between innate and adaptive immune cells at the maternal-fetal interface will occur prior to microbial-induced preterm labor. Using an established murine model of endotoxin-induced PTB, our results demonstrate that prior to delivery there is a reduction of CD4 ＋ regulatory T cells （Tregs） in the uterine tissues. This reduction is neither linked to a diminished number of Tregs in the spleen, nor to an impaired production of ILIO, CCL17, or CCL22 by the uterine tissues. Endotoxin administration to pregnant mice does not alter effector CD4＋ T cells at the maternal-fetal interface. However, it causes an imbalance between Tregs （CD4＋ and CD8＋）, effector CD8＋ T cells, and Th17 cells in the spleen. In addition, endotoxin administration to pregnant mice leads to an excessive production of CCL2, CCL3, CCL17, and CCL22 by the uterine tissues as well as abundant neutrophils. This imbalance in the uterine microenvironment is accompanied by scarce APC-like cells such as macrophages and MHC II ＋ neutrophils. Collectively, these results demonstrate that endotoxin administration to pregnant mice causes an imbalance between innate and adaptive immune cells at the maternal-fetal interface.

关键词： antigen-presenting cells effector T cells inflammation labor lipopolysaccharide macrophages microbial product neutrophils pregnancy preterm labor regulatory T cells

Heterotopic pregnancy after assisted reproductive techniques with favorable outcome of the intrauterine pregnancy:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第3期11卷 669-676页

作者： Ya-Nan Wang Lian-Wen Zheng Lu-Lu Fu Ying Xu Xue-Ying Zhang Department of Obstetrics and Gynecology Reproductive Medical CenterThe Second Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND Heterotopic pregnancy(HP)is a rare condition in which both ectopic and intrauterine pregnancies *** is uncommon after natural conception but has recently received more attention due to the widespread use of assisted reproductive techniques(ART)such as ovulation promotion *** SUMMARY Here,we describe a case of HP that occurred after ART with concurrent tubal and intrauterine singleton *** was treated successfully with surgery to preserve the intrauterine pregnancy,resulting in the birth of a low-weight premature *** case report aims to increase awareness of the possibility of HP during routine first-trimester ultrasound examinations,especially in pregnancies resulting from ART and even if multiple intrauterine pregnancies are *** This case alerts us to the importance of comprehensive data collection during regular *** is important for us to remind ourselves of the possibility of HP in all patients presenting after ART,especially in women with an established and stable intrauterine pregnancy that complain of constant abdominal discomfort and also in women with an unusually raised human chorionic gonadotropin level compared with simplex intrauterine *** will allow symptomatic and timeous treatment of patients with better results.

关键词： Heterotopic pregnancy Assisted reproductive techniques preterm labor Premature rupture of membranes Case report

Mental health impact on Black,Asian and Minority Ethnic populations with preterm birth:A systematic review and meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2022年第9期12卷 1233-1254页

作者： Gayathri Delanerolle Yutian Zeng Peter Phiri Thuan Phan Nicola Tempest Paula Busuulwa Ashish Shetty Vanessa Raymont Shanaya Rathod Jian-Qing Shi Dharani K Hapangama Nuffield Department of Primary Care Health Science University of OxfordOxford OX37JXUnited Kingdom Research and Innovation Southern Health NHS Foundation TrustSouthampton SO303JBUnited Kingdom Southern University of Science and Technology Shenzhen 518055Guangdong ProvinceChina Psychology Department Faculty of Environmental and Life SciencesUniversity of SouthamptonSouthampton SO171BJUnited Kingdom Department of Women's and Children's Health University of LiverpoolLiverpool L78TXUnited Kingdom Gynaecology Directorate and Hewitt Centre for Reproductive Medicine Liverpool Women's NHS FoundationLiverpool L87SSUnited Kingdom National Hospital for Neurology and Neurosurgery University College London Hospitals NHS Foundation TrustLondon W1T 4AJUnited Kingdom Department of Psychiatry University of OxfordOxford OX37JXUnited Kingdom National Centre for Applied Mathematics Shenzhen Shenzhen 518055Guangdong ProvinceChina Department of Statistics Southern University of Science and TechnologyShenzhen 518055Guangdong ProvinceChina

BACKGROUND preterm birth(PTB)is one of the main causes of neonatal deaths globally,with approximately 15million infants are born *** from the Black,Asian,and Minority Ethnic(BAME)populations maybe at higher risk of PTB,therefore,the mental health impact on mothers experiencing a PTB is particularly important,within the BAME *** To determine the prevalence of mental health conditions among BAME women with PTB as well as the methods of mental health assessments used to characterise the mental health *** A systematic methodology was developed and published as a protocol in PROSPERO(CRD420-20210863).Multiple databases were used to extract relevant data.I2 and Egger's tests were used to detect the heterogeneity and publication bias.A trim and fill method was used to demonstrate the influence of publication bias and the credibility of *** Thirty-nine studies met the eligibility criteria from a possible *** prevalence rates of depression among PTB-BAME mothers were significantly higher than full-term mothers with a standardized mean difference of 1.5 and a 95%confidence interval(CI)29%-74%.The subgroup analysis indicated depressive symptoms to be time *** within the very PTB category demonstrated a significantly higher prevalence of depression than those categorised as non-very *** prevalence rates of anxiety and stress among PTB-BAME mothers were significantly higher than in full-term mothers(odds ratio of 88%and 60%with a CI of 42%-149%and 24%-106%,respectively).CONCLUSION BAME women with PTB suffer with mental health *** studies did not report on specific mental health outcomes for BAME ***,the impact of PTB is not accurately represented in this population,and thus could negatively influence the quality of maternity services they receive.

关键词： preterm labor preterm birth Black Asian and Minority Ethnic Mental health Women's health Wellbeing

Future directions of clinical laboratory evaluation ol pregnancy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2014年第6期11卷 582-588页

作者： Kenneth D Beaman Mukesh K Jaiswal Svetlana Dambaeva Alice Gilman-Sachs Department of Microbiology and Immunology Rosalind Franklin University of Medicine and Science North Chicago IL USA

In recent years, our understanding of how the immune system interacts with the developing fetus and placenta has greatly expanded. There are many laboratories that provide tests for diagnosis of pregnancy outcome in women who have recurrent pregnancy loss （RPL） or pre-eclampsia. These tests are based on the premise that immune response to the fetus is equivalent to the adaptive immune response to a transplant. New understanding leads to the concept that the activated innate response is vital for pregnancy and this can result in more effective testing and treatment to prevent an abnormal pregnancy in the future. We describe here only three such areas for future testing： one area involves sperm and semen and factors necessary for successful fertilization; another area would determine conditions for production of growth factors necessary for implantation in the uterus; finally, the last area would be to determine conditions necessary for the vascularization of the placenta and growing fetus by activated natural killer （NK） cells （combinations of killer cell immunoglobulin-like receptor （KIR） family genes with HLA-C haplotypes） that lead to capability of secreting angiogenic growth factors. These areas are novel but understanding their role in pregnancy can lead to insight into how to maintain and treat pregnancies with complicating factors.

关键词： male factor preterm labor recurrent pregnancy loss seminal plasma sperm

Expression of Toll-Like Receptor-2 and -4 in Amniochorion Membranes of preterm Delivery in the Presence of Histologic Chorioamnionitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Obstetrics and Gynecology 2014年第14期4卷 857-863页

作者： Natália Prearo Moco Laura Fernandes Martin Jossimara Polettini Ana Carolina Pereira José Carlos Peracoli Márcia Guimaraes da Silva Department of Pathology Botucatu Medical School (FMB) Sao Paulo State University (UNESP) Botucatu Brazil Department of Gynecology and Obstetrics Botucatu Medical School (FMB) Sao Paulo State University (UNESP) Botucatu Brazil

Expression of Toll-like receptors at the maternal-fetal interface during normal and complicated pregnancies has aroused interest in the last few years. However, despite the importance of TLR-2 and TLR-4, which recognizes most microorganisms presenting in the amniotic cavity infections associated with prematurity, comparison of the expressions of these receptors is rare in the literature. Thus, the purpose of this study was to compare the gene expression between TLR-2 and TLR-4 in amniochorion membranes of pregnant women with preterm delivery in the presence of histologic chorioamnionitis (HCA). Amniochorion membranes were collected from 40 pregnant women with preterm delivery;20 presented HCA and 20 did not. Fragments of the membranes were submitted to total RNA extraction, followed by cDNA production by reverse transcription. Real time quantitative PCR was used to quantify the gene expression of the TLRs. mRNA concentrations between TLR-2 and TLR-4 were compared using the nonparametric Mann-Whitney test. TLR-2 expression was higher than TLR-4 expression in the presence of HCA. No difference was observed between TLR-2 and TLR-4 expression in membranes in the absence of inflammatory infiltrate. In conclusion, amniochorion membranes express TLR-2 and TLR-4 and higher TLR-2 expression in the presence of histologic chorioamnionitis suggests that microorganisms recognizable by TLR-2 play an important role in the physiopathology of preterm labor.

关键词： preterm labor Pregnancy Complications Innate Immunology TLR-2 TLR-4

Pregnancy Outcomes in Pregnant Patients with COVID-19: A Multicenter Study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Obstetrics and Gynecology 2023年第1期13卷 13-25页

作者： Mufareh Asiri Salem Al Suwaidan Afaf Altweijry Abdulsamed Almdefa Saad Alonze Theba Saud Maternity Hospital King Saud Medical City Riyadh KSA Research Center King Saud Medical City Riyadh KSA

Introduction: Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was declared a pandemic by the World Health Organization (WHO) on March 11, 2020. The presence of COVID-19 in a pregnant patient can raise concerns, as other types of coronaviruses were associated with many adverse outcomes. This study aims to study the effect of COVID-19 on pregnancy outcomes. Methods: A prospective cross-sectional cohort study within Central First Health Care Cluster (multicentric), Riyadh, included all pregnant women with a singleton pregnancy diagnosed as COVID-19-positive. The primary outcome is the severity of COVID during pregnancy in terms of ICU admission and mortality. The participants were divided into three groups (preterm less than 37 weeks, the term from 37 - 40 weeks, and late-term after 40 weeks. In addition, parameters included: Gestational age at diagnosis, symptoms at presentation (cough, fever), presence of congenital anomalies, IUFD, mode of delivery, presence of PPH, newborn Apgar score, cord PH, need for NICU admission, and the newborn becoming infected with COVID-19 were also measured as secondary outcomes. Results: One hundred pregnant, COVID-19-positive women met the inclusion criteria;the average age of participants was 31.2 years (SD ± 6.4). Asymptomatic patients represented 54% of participants. Most of the deliveries occurred at 36 weeks or less as preterm delivery. Cesarean sections represented 55% of our population. Four-term mothers (12.5%), more than 37 weeks, need ICU admission compared to 13 (25%) preterm cases diagnosed with preeclampsia. No maternal death. Conclusion: COVID-19 during pregnancy can increase ICU admission. A high rate of preterm labor, miscarriage, cesarean section, and newborn testing positive for COVID-19 were observed among our population. No congenital anomalies related to COVID-19 were observed.

关键词： Pregnancy COVID-19 preterm labor Miscarriage Saudi Arabia

Trichomoniasis in Pregnant Sudanese Pregnant Women at Tertiary Obstetric Facility, Khartoum, Sudan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Obstetrics and Gynecology 2016年第9期6卷 539-543页

作者： Hanan A. O. Abd Allha A. Fazari Khalid Y. M. Ahmed Reproductive and Child Health Research Unit University of Medical Sciences & Technology Khartoum Sudan Omdurman Maternity Hospital Ministry of Health Khartoum Sudan Latifa Hospital Dubai Health Authority Dubai UAE Faculty of Medicine Alnileen University Khartoum Sudan

Background: Trichomoniasis is a common Sexually Transmitted Disease (STD) and the most common curable one. Methodology: This was cross sectional, prospective, total coverage hospital based study. It aimed to determine the incidence, risk factors of the Trichomoniasis among the laboring Sudanese women at Omdurman New Hospital Khartoum-Sudan, from September 2012 to April 2013. Data were obtained, entered and analyzed using soft word package program for social science (SPSS) version 17. Results: The prevalence of Trichomoniasis is 6.8%. It was found that the awareness about the infection is very low only 35%. Diabetes and history of sexual transmitted infections are the risks seen among the study cases. preterm labor was seen significantly. Conclusion: Syndromic management of all women with abnormal vaginal discharge is important since it causes reproductive morbidity.

关键词： Trichomoniasis Low Birth Weight preterm labor