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The egg cell is preferentially fertilized in Arabidopsis double fertilization

Journal of Integrative Plant Biology 2022年第11期64卷 2039-2046页

作者： Ling Li Saiying Hou Wei Xiang Zihan Song Yuan Wang Li Zhang Jing Li Hongya Gu Juan Dong Thomas Dresselhaus Sheng Zhong Li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking‐Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design College of Bioscience and Resources EnvironmentBeijing University of AgricultureBeijing 102206China The National Plant Gene Research Center Beijing 100101China The Waksman Institute of Microbiology Rutgers the State University of New JerseyPiscatawayNJ"2"08854USA Cell Biology and Plant Biochemistry University of RegensburgRegensburg 93053Germany

In flowering plants(angiosperms),fertilization of the egg cell by one sperm cell produces an embryo,whereas fusion of a second sperm cell with the central cell generates the *** most angiosperms like Arabidopsis,a pollen grain contains two isomorphic sperm cells required for this double fertilization process.A long-standing unsolved question is whether the two fertilization events have any preference.A tool to address this question is the usage of the cyclin-dependent kinase a1(cdka;1)mutant pollen,which produces a single sperm-like cell(SLC).Here,we first adopt a complementation-based fluorescence-labeling method to successfully separate and collect cdka;1 mutant pollen containing a single ***-cell RNA-sequencing analysis revealed that cdka;1 SLCs show a gene expression profile highly similar to that of sperm cells and not to the generative cell,precursor of the two sperm *** assays using a limited number of cdka;1 mutant pollen revealed that in 98.2%of the ovules,single fertilization of the egg cell *** of pistils with excessive cdka;1 mutant pollen allowed the delivery of a second SLC via fertilization recovery,which fertilized the central cell,resulting in 20.7%double-fertilized *** indicates that cdka;1 SLCs are able to fertilize both the egg and the central *** together,our findings have answered a long-standing question and support that preferential fertilization of the egg cell is evident in Arabidopsis.

关键词： Arabidopsis double fertilization egg cell preferential fertilization

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Egg cell-secreted aspartic proteases ECS1/2 promote gamete attachment to prioritize the fertilization of egg cells over central cells in Arabidopsis

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Journal of Integrative Plant Biology 2022年第11期64卷 2047-2059页

作者： Jiahao Jiang Nils Stührwohldt Tianxu Liu Qingpei Huang Ling Li Li Zhang Hongya Gu Liumin Fan Sheng Zhong Andreas Schaller Li-Jia Qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China Department of Plant Physiology and Biochemistry Institute of BiologyUniversity of HohenheimStuttgart 70593Germany

Double fertilization is an innovative phenomenon in angiosperms,in which one sperm cell first fuses with the egg cell to produce the embryo,and then the other sperm fuses with the central cell to produce the ***,the molecular mechanism of the preferential fertilization of egg cells is poorly *** this study,we report that two egg cell-secreted aspartic proteases,ECS1 and ECS2,play an important role in promoting preferential fertilization of egg cells in *** show that simultaneous loss of ECS1 and ECS2 function resulted in an approximately 20%reduction in fertility,which can be complemented by the full-length ECS1/2 but not by corresponding active site mutants or by secretion-defective versions of ECS1/*** phenotypic analysis revealed that the egg cellsperm cell attachment was compromised in ecs1 ecs2 *** pollination assays with cyclin-dependent kinase a1(cdka;1)pollen showed that preferential egg cell fertilization was impaired in the ecs1 ecs2 *** together,these results demonstrate that egg cells secret two aspartic proteases,ECS1 and ECS2,to facilitate the attachment of sperm cells to egg cells so that preferential fertilization of egg cells is *** study reveals the molecular mechanism of preferential fertilization in Arabidopsis thaliana.

关键词： Arabidopsis thaliana double fertilization ECS1/2 gamete interaction preferential fertilization

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