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A simple and precise method(Y2H-in-frame-seq) improves yeast two-hybrid screening with cDNA libraries

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Journal of Genetics and Genomics 2022年第6期49卷 595-598页

作者： Yinghui Gu Guannan Li Ping Wang Yan Guo Jingrui Li State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

As the most powerful and widely used binary interaction assay,the yeast two-hybrid(Y2H) system has been developed for more than 30 years to improve the efficiency and quality of proteinprotein interaction(PPI) explora... 详细信息

关键词： precise interaction improve

Highly sensitive H_(2)O_(2)-scavenging nano-bionic system for precise treatment of atherosclerosis

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Acta Pharmaceutica Sinica B 2023年第1期13卷 372-389页

作者： Xiaoyu Liang Huiyang Li Xuanling Li Xinxin Tian Aiai Zhang Qingzhi Luo Jianwei Duan Youlu Chen Liyun Pang Chen Li Xing-Jie Liang Yong Zeng Jing Yang Tianjin Key Laboratory of Biomaterial Research Institute of Biomedical EngineeringChinese Academy of Medical Science and Peking Union Medical CollegeTianjin 300192China Beijing Anzhen Hospital of Capital Medical University Beijing 100029China Medical College of Qinghai University Xining 810016China CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology of ChinaBejing 100190China The First Affiliated Hospital of Hebei North University Zhangjiakou 075061China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300381China

In atherosclerosis,chronic inflammatory processes in local diseased areas may lead to the accumulation of reactive oxygen species(ROS).In this study,we devised a highly sensitive H_(2)O_(2)-scavenging nanobionic system loaded with probucol(RPP-PU),to treat atherosclerosis more effectively.The RPP material had high sensitivity to H_(2)O_(2),and the response sensitivity could be reduced from 40 to 10μmol/L which was close to the lowest concentration of H_(2)O_(2)levels of the pathological environment.RPP-PU delayed the release and prolonged the duration of PU in vivo.In Apolipoprotein E deficient(ApoE-/-)mice,RPP-PU effectively eliminated pathological ROS,reduced the level of lipids and related metabolic enzymes,and significantly decreased the area of vascular plaques and fibers.Our study demonstrated that the H_(2)O_(2)-scavenging nanobionic system could scavenge the abundant ROS in the atherosclerosis lesion,thereby reducing the oxidative stress for treating atherosclerosis and thus achieve the therapeutic goals with atherosclerosis more desirably.

关键词： Highly sensitive H_(2)O_(2)-scavenging Nano-bionic system Atherosclerosis Oxidative stress precise Target Pathological environment

Phase transferring atomically precise gold nanoclusters to aqueous solution via single stranded DNA

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Science China Chemistry 2022年第6期65卷 1003-1004页

作者： Qiangbin Wang CAS Key Laboratory of Nano-Bio Interface Division of Nanobiomedicine and i-LabSuzhou Institute of Nano-Tech and Nano-BionicsSuzhou215123China

Atomically precise gold nanoclusters (Au NCs) are an emerging class of quantum-sized nanomaterials. The intrinsic discrete electronic energy levels endowed them with fascinating electronic and optical properties [1]. However,the prepared Au NCs are often protected with hydrophobic ligands and are not dispersible in aqueous solutions, which restricts their biological applications. To overcome this limitation, some phase transfer agents have been devoted to transferring hydrophobic Au NCs into the aqueous solution[2]. However, the resulting Au NCs are generally heterogeneous and aggregated, which makes their subsequent biofunctionalization difficult to control.

关键词： materials precise clusters

Surface ligand engineering on the optical properties of atomically precise AuAg nanoclusters

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Chinese Journal of Structural Chemistry 2023年第9期42卷 1-5页

作者： Xiang Liu Yifei Zhang Zhiwen Li Gao Li Farid Taherkhani Hunan Drug Inspection Center Hunan Institute for Drug ControlChangsha410013China State Key Laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Institute of Catalysis for Energy and Environment College of Chemistry and Chemical EngineeringShenyang Normal UniversityShenyang110034China University of Chinese Academy of Sciences Beijing100049China Departments of Production Engineering Universität Bremen Bibliothekstraße 128359Germany

1.Introduction In recent decade,atomically precise metal nanoclusters,comprised of metal kernel and surface protecting ligands,have appeared as a new and promising nanomaterial for abundant applications,such as nanocatalysis,sensor,photoluminescence,a few to name,which is attributed to their electron energy quantization arising from quantum size effect[1].And the very recent investigations showed that the configuration of metal kernel and the electronic properties of metal nanoclusters with the same metal core are largely affected by the surface protecting ligands[[2],[3]].For example,the surface ligands can significantly alter the catalytic performance of metal nanocluster catalysts(e.***.activity and selectivity)in a positive way.We have found that the Au_(25)(SR)_(18)nanocluster with the aromatic ligands can give higher conversions and a markedly increasing selectivity toward the desired product[4].

关键词： precise clusters selectivity

HIGH-VOLTAGE VANGUARD One company's trailblazing path in China's electric vehicle evolution

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Beijing Review 2024年第3期67卷 16-17页

作者： Zhao Wei 不详

Towering robots with arms extending over 3 meters perform a precise,choreographed dance within the confines of the SERES smart factory.Amid a cascade of welding sparks,they effortlessly manipulate heavy metal plates,making building fullsize vehicles look like assembling model cars.This futuristic spectacle marks a drastic shift from traditional automobile manufacturing,where human labor plays a major role.

关键词： trail precise evolution

Efficient Improvement of Nutritional Content in Rice Grains by precise Base Editing of OsROS1

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Rice science 2023年第6期30卷 499-503页

作者： XU Yang WANG Fangquan LI Wenqi WANG Jun TAO Yajun FAN Fangjun CHEN Zhihui JIANG Yanjie ZHU Jianping LI Xia ZHU Qianhao YANG Jie Key Laboratory of Germplasm Innovation in Downstream of Huaihe River Ministry of Agriculture and Rural Affairs/Institute of Food CropsJiangsu Academy of Agricultural SciencesNanjing 210014China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009China Institute of Life Science Jiangsu UniversityZhenjiang 212013China Commonwealth Scientific and Industrial Research Organisation Agriculture and Food GPO Box 1700CanberraACT 2601Australia

Aleurone forms the outermost layer of the rice endosperm and plays a critical role in apoplastic nutrient uptake during endosperm development.Thickening the aleurone layer has been proposed to significantly increase the nutrient content of rice grains.In this study,we used a CRISPR/Cas9-mediated precise base editing method to target OsROS1,a gene associated with aleurone thickness,in the background of the japonica glutinous rice cultivar Zhennuo 19。

关键词： CRISPR/Cas9 cultivar precise

Gene-like precise Construction of Functional DNA Materials

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Accounts of Materials Research 2022年第1期3卷 42-53页

作者： Chi Yao Yuwei Xu Pin Hu Junhan Ou Dayong Yang Frontiers Science Center for Synthetic Biology Key Laboratory of Systems Bioengineering(MOE)Institute of Biomolecular and Biomedical EngineeringSchool of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350P.R.China

CONSPECTUS:Developing a new system of material chemistry is an important molecular foundation for the design and preparation of functional materials,which resembles the preparation of raw materials such as bricks for constructing a building that determines the quality of the building.However,precisely controlling the synthesis processes and structures of functional materials always remains a grand challenge.It is expected to propose a paradigm of“gene-like”precise construction to realize the rational synthesis of functional materials,i.e.,a“structure-function-application”principle for the precise construction.As the core genetic material of life,the DNA molecule is a bioactive macromolecule that can accurately encode genetic information and also can act as the generic molecular building block for the precise construction of functional materials.In this Account,we describe our work on the design and construction of functional DNA materials,to illustrate the principle of“gene-like”construction of functional DNA materials.The DNA molecule shows the unique advantages in the“gene-like”construction of functional materials,mainly including the precise arrangement of bases(monomers),the controllable design and assembly of structure,the precise transmission of sequence information,and customization of function.First,the number and sequence of four deoxynucleotide monomers that constitute the DNA strand can be rationally designed and accurately synthesized.Second,the sequence information on DNA endows the precise and efficient assembly of DNA molecules and ensures the precise regulation on the specific biological functions of functional DNA materials.Third,the functions of DNA materials can couple with the biological environments,so as to achieve the predetermined applications.Based on the scale of the constructed DNA materials,we categorize DNA materials into three classes:molecular scale,nanoscale,and macroscale.At the molecular scale,the representative material is branched DNA-based materials;the construction strategies

关键词： materials. precise synthesis

Construction of molecular nanotubes with precise length,diameter and chirality

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中国化学快报:英文版 2023年第9期34卷 3-4页

作者： Qiaona Zhang Hongwei Qian Tangxin Xiao Robert B.P.Elmes Leyong Wang School of Petrochemical Engineering Changzhou UniversityChangzhou 213164China Department of Chemistry Maynooth UniversityNational University of IrelandMaynoothCo.KildareIreland State Key Laboratory of Analytical Chemistry for Life Science Jiangsu Key Laboratory of Advanced Organic MaterialsSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Molecular nanotubes are nanoscale organic materials with tubular architecture,that show potential applications in molecular recognition/separation,cross-membrane transportation,catalysis in confined spaces,and nanoelectronics[1].However,the precise construction of molecular nanotubes with well-defined cavity sizes and shapes is non-trivial.

关键词： precise sizes molecular

Combining pMINFLUX,graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy

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Light(Science & Applications) 2023年第4期12卷 585-592页

作者： Jonas Zähringer Fiona Cole Johann Bohlen Florian Steiner Izabela Kamińska Philip Tinnefeld Department of Chemistry and Center for NanoScience Ludwig-Maximilians-Universität MünchenButenandtstr.5-13 Haus E81377 MünchenGermany Institute of Physical Chemistry Polish Academy of Sciences Kasprzaka 44/5201-224WarsawPoland Department of Physics Ludwig-Maximilians-Universität MünchenSchellingstra■e 480799 MünchenGermany

3D super-resolution microscopy with nanometric resolution is a key to fully complement ultrastructural techniques with fluorescence imaging.Here,we achieve 3D super-resolution by combining the 2D localization of pMINFLUX with the axial information of graphene energy transfer(GET)and the single-molecule switching by DNA-PAINT.We demonstrate≤2 nm localization precision in all 3 dimension with axial precision reaching below 0.3 nm.In 3D DNA-PAINT measurements,structural features,i.e.,individual docking strands at distances of 3 nm,are directly resolved on DNA origami structures.pMINFLUX and GET represent a particular synergetic combination for super-resolution imaging near the surface such as for cell adhesion and membrane complexes as the information of each photon is used for both 2D and axial localization information.Furthermore,we introduce local PAINT(L-PAINT),in which DNA-PAINT imager strands are equipped with an additional binding sequence for local upconcentration improving signal-to-background ratio and imaging speed of local clusters.L-PAINT is demonstrated by imaging a triangular structure with 6 nm side lengths within seconds.

关键词： resolution transfer precise