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Perineuronal nets increase inhibitory GABAergic currents during the critical period in rats

International Journal of Ophthalmology(English edition) 2013年第2期6卷 120-125页

作者： Hui Liu Peng-Fen Gao Hai-Wei Xu Ming-Ming Liu Tao Yu Jun-Ping Yao Zheng-Qin Yin Southwest Eye Hospital Southwest Hospital Third Military Medical University Key Lab of Visual Damage and Regeneration & Restoration of Chongqing

AIM: To investigate inhibitory γ-aminobutyric acid (GABA) ergic postsynaptic currents (IPSCs) and postsynaptic currents (PSCs) in layer IV of the rat visual cortex during the critical period and when plasticity was extended through dissolution of the perineuronal nets (PNNs). METHODS: We employed 24 normal Long-Evans rats to study GABA A-PSC characteristics of neurons within layer IV of the visual cortex during development. The animals were divided into six groups of four rats according to ages at recording: PW3 (P21 -23d), PW4 (P28 -30d), PW5 (P35-37d), PW6 (P42-44d), PW7 (P49-51d), and PW8 (56-58d). An additional 24 chondroitin sulfate proteoglycan (CSPG) degradation rats (also Long-Evans) were generated by making a pattern of injections of chondroitinase ABC (chABC) into the visual cortex 1 week prior to recording at PW3, PW4, PW5, PW6, PW7, and PW8. Immunohistochemistry was used to identify the effect of chABC injection on CSPGs. PSCs were detected with whole-cell patch recordings, and GABA A receptor-mediated IPSCs were pharmacologically isolated. RESULTS: IPSC peak current showed a strong rise in the age-matched control group, peaked at PW5 and were maintained at a roughly constant value thereafter. Although there was a small increase in peak current for the chABC group with age, the peak currents continued to decrease with the delayed highest value at PW6, resulting in significantly different week-by-week com-parison with normal development. IPSC decay time continued to increase until PW7 in the control group, while those in the chABC group were maintained at astable level after an initial increase at PW4. Compared with normal rats, the decay times recorded in the chABC rats were always shorter, which differed significantly at each age. We did not observe any differences in IPSC properties between the age-matched control and penicillinase (P-ase) group. However, the change in IPSCs after chABC treatment was not reflected in the total PSCs or in basic membrane

关键词： gamma-aminobutyric acid receptor plasticity visual cortex development postsynaptic currents chondroitinase ABC chondroitin sulfate proteoglycans whole-cell patch recording

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Properties of synaptic responses evoked by tactile stimulus in Purkinje cells in mice cerebellar cortex crus Ⅱ in vivo

Properties of synaptic responses evoked by tactile stimulus ...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Chun-Ping CHU~(1,3),Yan-Hua BING~(1,2),Quan-Ri LIU~(1,2),De-Lai QIU~(1,2,*) 1 Cellular Function Research Center,Yanbian University 2 Department of Physiology and Pathophysiology 3 Function Experiment Center,College of Basic Medicine,Yanbian University,977,GongYuan Road,Yanji 133002,China.

Sensory stimuli evoke responses in cerebellar Purkinje cells(PCs) via mossy fiber-granule cell pathway. However,the properties and characterization of synaptic responses evoked by the tactile stimulus in cerebella PCs are *** the present study,we investigated the synaptic responses of cerebellar PCs in response to the airpuff stimulus on the ipsilateral whisker pad in urethane-anesthetized mice.A total of 33 PCs from 35 adult(6-8 weeks old) ICR mice were studied by somatic or dendritic patch-clamp recording with biolytic staining and pharmacological *** current-clamp conditions(I = 0),air-puff stimulus evoked strong inhibitory postsynaptic potentials (IPSPs) in PCs *** of SR95531,a specific GABA receptor antagonist,blocked IPSPs,and revealed the stimulus-evoked simple spike *** voltage-clamp conditions,tactile stimulus evoked a sequence of transient inward currents followed by strong inhibitory postsynaptic currents(IPSCs) in both PCs stomata and *** of GABA receptor antagonist,SR95531 blocked IPSCs,revealed the excitatory postsynaptic currents(EPSCs) in somata and a temporal summation of parallel-fiber EPSCs in dendrites of *** also demonstrated that PCs respond to both onset and offset of air-puff *** findings indicated that the tactile stimulus could not evoke the strong EPSCs and fire PCs,but induced the rapid activation of strong GABA rereptormediated IPSCs in both stomata and dendrites of *** propose that the inhibition play a crucial role in controlling the output of sensory information in PCs in cerebellar cortex CrusⅡin mice.

关键词： cerebellar cortex in vivo patch-clamp Purkinje cell tactile stimulation postsynaptic currents GABAA receptor

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