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Polydopamine-assisted functionalization of heparin and vancomycin onto microarc-oxidized 3D printed porous ti6al4v for improved hemocompatibility,osteogenic and anti-infection potencies

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Science China Materials 2018年第4期61卷 579-592页

作者： Teng Zhang Wenhao Zhou Zhaojun Jia Qingguang Wei Daoyang Fan Jianglong Yan Chuan Yin Yan Cheng Hong Cai Xiaoguang Liu Hua Zhou Xiaojie Yang Yufeng Zheng Zhongjun Liu Department of Orthopedics Peking University Third HospitalBeijing 100191China Beijing Key Laboratory of Spinal Diseases Beijing 100191China Center for Biomedical Materials and tissue Engineering Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijing 100871China Beijing AKEC Medical Company Ltd. Beijing 102200China

Enhanced antiinfection activities, improved hemocompatibility and osteo-compatibility, and reinforced osseointegration are among the most important considerations in designing multifunctional orthopedic ***, anti-infective and osteogenic multifunctional 3 D printed porous ti6 al4 v implant with excellent hemocompatibility was successfully designed and fabricated. In brief, osteogenic micro-arc oxidation（MAO） coatings with micro/nanoscale porous topography were generated in situ on3 D printed ti6 al4 v scaffolds, on which heparin and vancomycin were easily immobilized. The surface microstructure,morphology, and chemical compositions were characterized employing scanning electron microscopy（SEM）, X-ray photoelectron spectroscopy（XPS） and Fourier transform infrared spectroscopy（FtiR）. High loading capacity and sustained vancomycin release profiles were revealed using high performance liquid chromatography（HPLC）. Favorable antibacterial and antibiofilm performances against pathogenic Staphylococcus aureus（S. aureus） were validated in vitro through microbial viability assays, Live/Dead bacterial staining, and crystal violet staining. Human mesenchymal stem cells（h MSCs） were seeded on the scaffolds and their proliferation and viability were assessed using Cell Counting Kit and Live/Dead cell viability kit. Further, osteoblastic differentiation abilities were evaluated using alkaline phosphatase（alP） activity as a hall marker. Additionally, the improved hemocompatibility of the heparinized scaffolds was confirmed by activated partial thromboplastin time（APTT）, prothrombin time（PT） and thrombin time（TT）. Overall, our results show that the surface-modified 3 D printed porous ti6 al4 v possesses balanced antibacterial and osteogenic functions while exhibiting extra anticlotting effects, boding well for future application in customized functional reconstruction of intricate bone defects.

关键词： 3D printing porous ti6al4v anti-infection microarc oxidation osseointegration vancomycin

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维普期刊数据库

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Hierarchical Micropore/Nanorod Apatite Hybrids In-Situ Grown from 3-D Printed Macroporous ti6al4v Implants with Improved Bioactivity and Osseointegration

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Journal of Materials Science & Technology 2017年第2期33卷 179-186页

作者： Peng Xiu Zhaojun Jia Jia Lv Chuan Yin Hong Cai Chunli Song Huijie Leng Yufeng Zheng Zhongjun Liu Yan Cheng Department of Orthopedics Peking University Third HospitalBelting 100191China Beijing Key Laboratory of Spinal Diseases Beijing 100191China Center for Biomedical Materials and tissue Engineering Academy for Advanced Interdisciplinary Studies.Peking UniversityBeijing 100871China Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijing 100871China

The advent of three-dimensional （3-D） printed technique provides great possibility in the fabrication of customized porous titanium （ti） implant. However, the bioinert property of the printed ti poses an out- standing problem. Hybrid micro-arc oxidation and hydrothermal （MAO-HT） treatment on porous metals is able to produce multi-scaled hierarchical orthopedic implant, showing great potential for surface mod- ification of 3-D printed implant. In this study, cylindrical porous ti6al4v （ti64） scaffolds with pore size of 640 lure, porosity of ？3% were 3-D printed by electron beam melting process, and their surfaces were left untreated or treated by a combined MAO-HT procedure. In vitro bioactivity was tested by immer- sion in simulated body fluid for different time points. Then, 12 scaffolds in each group were implanted into the femoral condyles of New Zealand rabbit for 8 weeks. Osseointegration was evaluated by qual- itative and quantitative histological analysis, and the bone ingrowth features were probed by sequential fluorescent labeling at 3 and 6 weeks post-surgery, Following the MAO-HT treatment, the porous ti64 scaffold was endowed with multi-scaled micro/nano-topographies and high amounts of CaP on its surface. The treated scaffold exhibited drastically enhanced apatite forming ability compared with the un- treated one. In vivo test revealed significantly that a higher amount of bone ingrowth and bone implant contact at the treated scaffold. The 2 types of scaffolds had different patterns of bone ingrowth; the treated scaffold exhibited a pattern of contact osteogenesis, by which bone formed directly on the treated implant surface, whereas bone formed distal to the implant surface of the untreated scaffold. MAO-HT treat- ment can significantly enhance the in vitro apatite-inducing ability and in vivo osseointegration capacity of 3-D porous ti64 scaffold and may provide as a viable approach for the fabrication of bioactive 3-D printed porous implant for orthop

关键词： 3-D printing porous ti6al4v Micro-arc oxidation Hydrothermal treatment Osseointegration

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