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Comparative Analysis of Cry1Ac Toxin Oligomerization and pore formation Between Bt-Susceptible and Bt-Resistant Helicoverpa armigera Larvae

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Journal of Integrative Agriculture 2012年第11期11卷 1845-1851页

作者： LI Yi-ping WU Jun-xiang LIU Chen-xi YUAN Xiang-qun WU Kong-ming LIANG Ge-mei GUO Yu-yuan Key Laboratory of Applied Entomology " Key Laboratory of Plant Protection Resources and Pest Management Ministry of Education~ College of Plant Protection Northwest Agriculture and Forestry University Yangling 712100 P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R.China

With the long-term use of Bacillus thuringiensis （Bt） insecticide and expansion of CrylA-expressing transgenic plants, some insect pests have developed resistance to Bt in open fields, greenhouses, and in the laboratory. Bt resistance is complex and there appear to be different ways for resistance development. Understanding the Bt resistance mechanisms is critical to prolong its usefulness. In this article, Bt receptors, the cadherin and aminopeptidase N （APN）, in brush border membrane vesicles （/3BMV） of Helicoverpa armigera were examined in both CrylAc-susceptible （96S） and CrylAc- resistant （LF120） strains, to compare CrylAc toxin oligomerization and pore formation in these two strains. CrylAc toxin oligomerization and pore formation in these two strains were compared. Results showed that cadherin and aminopeptidase N proteins could express normally in both susceptible and resistant 11. armigera strains. The ability to form CrylAc oligomers and ion channels on BBMVs was also not significantly different between these two strains.

关键词： Helicoverpa armigera Bt resistance oligomerization pore formation

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Preparation and properties of SiC honeycomb ceramics by pressureless sintering technology

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Journal of Advanced Ceramics 2014年第1期3卷 83-88页

作者： Xingzhong GUO Xiaobo CAI Lin ZHU Lingjie ZHANG Hui YANG Department of Materials Science and Engineering Zhejiang UniversityHangzhou 310027China

SiC honeycomb ceramics with parallel channels and macroporous walls were prepared by combining extrusion molding with pressureless sintering technology in the presence of *** extrusion molding constructed honeycomb structure with parallel channels,while the starch formed spherical macropores on the channel *** density and bending strength of SiC honeycomb ceramics decreased with the increase of starch content,while the phase compositions did not vary with the starch *** control in starch addition could adjust the pore structures on the channel walls of SiC honeycomb ceramics.

关键词： honeycomb ceramic porous ceramic silicon carbide(SiC) pore formation

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Applications of atomic force microscopy in immunology

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Frontiers of Medicine 2021年第1期15卷 43-52页

作者： Jiping Li Yuying Liu Yidong Yuan Bo Huang Beijing Smartchip Microelectronics Technology Company Limited Beijing 100192China Department of Immunology&National Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China Clinical Immunology Center Chinese Academy of Medical SciencesBeijing 100005China School of Microelectronics Tianjin UniversityTianjin 300072China Department of Biochemistry&Molecular Biology Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030China

Cellular mechanics,a major regulating factor of cellular architecture and biological functions,responds to intrinsic stresses and extrinsic forces exerted by other cells and the extracellular matrix in the *** mechanics also acts as a fundamental mediator in complicated immune responses,such as cell migration,immune cell activation,and pathogen *** principle of atomic force microscopy(AFM)and its three running modes are introduced for the mechanical characterization of living *** peak force tapping mode provides the most delicate and desirable virtues to collect high-resolution images of morphology and force *** a concrete description of AFM capabilities,three AFM applications are *** applications include the dynamic progress of a neutrophil-extracellular-trap release by neutrophils,the immunological functions of macrophages,and the membrane pore formation mediated by perforin,streptolysin O,gasdermin D,or membrane attack complex.

关键词： cellular mechanics atomic force microscopy neutrophil extracellular trap macrophage phagocytosis pore formation

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Effect of Sr content on porosity formation in directionally solidified Al-12.3wt.%Si alloy

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China Foundry 2014年第5期11卷 435-439页

作者： Liao Hengcheng Wu Yuna Fan Ran Wang Qigui Jiangsu Key Lab. for Advanced Metallic Materials School of Materials Science and Engineering Southeast University Materials Technology GM Global Powertrain EngineeringPontiac 48340-2920 USA

The influence of Sr addition on pore formation in directionally solidified Al-12.3wt.% alloy was investigated using X-ray detection, optical microscope, and SEM-EDX. Results indicate that addition of Sr significantly increases the number density and volume fraction of porosity. The considerable rise in volume fraction of porosity is attributed to the remarkable increase in the numbers of pores formed. It is found that Sr solute in liquid Al-Si al oy can diffuse into the oxide inclusions to form loose oxide aggregations which have more activity as the nucleation sites for porosity. Adding more Sr considerably increases the numbers of active nucleation sites. There is an obvious fluctuation of pore number density during steady state solidification, which is believed to be related to a fluctuation of local hydrogen supersaturation induced by the competition of pore nucleation and growth for hydrogen solute supplement.

关键词： Al-Si alloy porosity oxides pore formation strontium directional solidification

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