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Wet Oxidation Pretreatment of poplar waste for Enhancing Enzymatic Hydrolysis Efficiency

Paper And Biomaterials 2017年第2期2卷 8-17页

作者： GuiGan Fang ShanShan Liu KuiZhong Shen Yan Lin Institute of Chemical Industry of Forestry Products CAFNational Engineering Lab for Biomass Chemical UtilizationKey Lab of Forest Chemical EngineeringSFAKey Lab of Biomass Energyand Material of JiangsuNanjingJiangsu Province210042China Institute of New Technology of Forestry CAFBeijing100091China Collaboration Innovation Center for High Efficient Processing and Utilization of Forestry Resources Nanjing Forestry UniversityNanjingJiangsu Province210037China Qilu University of Technology Ji'nanShandong Province250353China

In this paper, we described the optimization of the wet oxidation pretreatment conditions to enhance enzymatic hydrolysis efficiency, using poplar waste from the stock section of a paper mill as the raw material. We showed that the optimal conditions of the pretreatment for poplar waste were an initial p H value of 10, a temperature of 195℃, a holding time of 15 min, and an oxygen pressure of 1.2 MPa. In this case, the yield of the obtained solid material produced by the process was 51.7% and the reducing sugar yield was 46.8%. The solid part obtained from the pretreatment process was hydrolyzed by cellulase L-10. The optimal enzymatic conditions were a temperature of 49℃, a duration time of 56 h, an enzyme dosage of 38 FPU/g at a p H value of 4.8, and a solid-to-liquor ratio of 1∶50. The resulting cellulose conversion rate reached 96.4% in terms of the pretreated substances. In addition, a chemical composition analysis of the poplar waste and pretreated material indicated that about 92% of the hemicelluloses and 43% of the lignin in the raw material were degraded and dissolved. In addition, the crystallization decreased from 57.5% to 54.8%. An obvious fibrillation of the fiber pretreated by the wet oxidization process was observed by SEM. Moreover, high-performance liquid chromatography(HPLC) results showed a high xylose content and monosaccharide degradation products in the pretreatment solution. In conclusion, the wet oxidation pretreatment process could efficiently degrade or remove the lignin and hemicellulose, as well as reduce the crystallinity of the lignocellulosic material, which resulted in animprovement of the enzymatic ability and an increase in the cellulose conversion rate.

关键词： poplar waste wet oxidation pretreatment enzymatic hydrolysis efficiency cellulase hydrolysis

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